Information report for OsWRKY62
Gene Details
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Functional Descriptions
- The expression analyses of the group IIa WRKY transcription factors in rice revealed that OsWRKY28, together with OsWRKY71, exhibit an early-induced expression prior to the late-induced expressions of OsWRKY62 and OsWRKY76.
- Here, we report that XA21 binds to a WRKY transcription factor, called OsWRKY62.
- These results imply that OsWRKY62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses.
- OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice.
- Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes.
- Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice.
- Salicylic acid (SA) and jasmonic acid (JA)/JA-Ile contents were increased in dsOW62/76 and knockout lines of individual OsWRKY62 and OsWRKY76 genes.
- These results indicate that OsWRKY62 and OsWRKY76 function as negative regulators of biosynthetic defense-related metabolites and provide evidence for an important role of phenylpropanoid pathway in SA production in rice.
- Background OsWRKY62 and OsWRKY76, two close members of WRKY transcription factors, function together as transcriptional repressors.
- OsWRKY62 is predominantly localized in the cytosol.
- However, we also observed that OsWRKY62 plays negative roles in defense against a compatible M.
- Accordingly, knocking out of OsWRKY62 in a non-Pi9 background decreased immunity against M.
- Pi9 binds to ANIP1 and OsWRKY62 to form a complex, which may help to keep Pi9 in an inactive state and weaken聽rice immunity.
Functional Keywords
Literature and News
- OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus . DOI: 10.1007/s11103-013-0032-5 ; PMID: 23462973
- The WRKY Gene Family in Rice (Oryza sativa) . DOI: 10.1111/j.1744-7909.2007.00504.x
- OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice . DOI: 10.1093/mp/ssn024 ; PMID: 19825552
- Cleavage and nuclear localization of the rice XA21 immune receptor . DOI: 10.1038/ncomms1932 ; PMID: 22735448
- Alternative Splicing of Rice WRKY62 and WRKY76 Transcription Factor Genes in Pathogen Defense . DOI: 10.1104/pp.15.01921 ; PMID: 27208272
- Transcription Factor WRKY62 Plays a Role in Pathogen Defense and Hypoxia-Responsive Gene Expression in Rice . DOI: 10.1093/pcp/pcw185 ; PMID: 27837098
- Metabolic and transcriptional alternations for defense by interfering OsWRKY62 and OsWRKY76 transcriptions in rice . DOI: 10.1038/s41598-017-02643-x ; PMID: 28559550
- Identification of a small set of genes commonly regulated in rice roots in response to beneficial rhizobacteria . DOI: 10.1007/s12298-020-00911-1 ; PMID: 33424163
- The CC-NB-LRR OsRLR1 mediates rice disease resistance through interaction with OsWRKY19 . DOI: 10.1111/pbi.13530 ; PMID: 33368943
- A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1 . DOI: 10.3389/fpls.2021.802758 ; PMID: 34956298
- OsWRKY62 and OsWRKY76 Interact with Importin α1s for Negative Regulation of Defensive Responses in Rice Nucleus . DOI: 10.1186/s12284-022-00558-4 ; PMID: 35184252
- The ANIP1-OsWRKY62 module regulates both basal defense and Pi9-mediated immunity against Magnaporthe oryzae in rice . DOI: 10.1016/j.molp.2023.03.001 ; PMID: 36872602
Gene Resources
- UniProt: Q6EPZ0
- EMBL: AK067834, AP005429, AP005784
- AlphaFoldDB: Q6EPZ0
- EnsemblPlants: Os09t0417800-01
- Gramene: Os09t0417800-01
- KEGG: dosa:Os09g0417800
- Orthologous matrix: MWITIIL
- InterPro: IPR003657, IPR036576, IPR044810
- PANTHER: PTHR31429, PTHR31429:SF84
- SUPFAM: SSF118290
- PROSITE: PS50811
- Gene3D: 2.20.25.80
- OrthoDB: Q6EPZ0
- SWISS-MODEL: Q6EPZ0
- eggNOG: ENOG502QR7M
Homologs
- Panicum virgatum: Pavir.2NG334200.1.p, Pavir.2KG335400.1.p
- Setaria italica: Seita.2G206100.1.p
- Setaria viridis: Sevir.2G214600.1.p
Sequences
cDNA Sequence
- >LOC_Os09g25070.1
AAAGTTCACGTTTTGGTCCCCCTCCTGTTCGGCGACGGGCGACGTACGGCGCCACTATAAGAAACGTGCCCTATTGCTGTCACGCCGTACTTCCTCCCGCCCACCGGGCAGCTAGCTTAGCTGCCGCCATGGACGACGACGGCGACGGCTCATCGTCACCGACCGACGACAGCGCCGCCGCCGGGCTTTTGCCGCTCTTCAGCCGATCGCCGGCCGAGGATCTTGAGGAGAAGCTGAGGCGGGCGATGGAGGAGAACGCGCGGCTGACGCGAGCGCTCGACGCCATTCTGGCCGGTCACCACGCCCACCAGCGCGCGCTCTTGGCGCCGTCGCTGTCGCCGCCGCCGCCTTCCGCCACAGCAAGGGCGCCGAGCGTGTCCACCAGCTGCGCGGCGAGGGAGGACGCCGCACCGGCGGTCGCCGCCGCCGCCGCGTCGACGGCATGTCCGTCGAGACAGCAGCCGCCGACCGCGGAGCCACGGCCCAAGGTCAGGACGGTCCGCGTGCGCGCCGACGCCGCCGACGCCACCGACGCCAACAGCATGGCGGAGACCGTGAAGGATGGGTACCAATGGAGGAAGTACGGGCAGAAGGTGACGCGTGACAACCCCTACCCTAGAGCTTACTTCCGCTGCGCATTCGCGCCGTCTTGCCCCGTCAAGAAGAAGCTCCAAAGATGTGCGGAGGATAGGTCAATGCTAGTGGCGACCTACGAGGGAGAACACAACCATGCCCTGTCCACGCAGACAACCGAATTCGTCGCCAGCGGCTGCACTACGAGCCAGCACGCCGGGGGGTCGTCGTCGTCGCCGCTGCCGTGCTCCATCTCCATCAACTCGTCGGGCCGCACGATCACACTCGACCTGACGAACCAAGCAGGGTCCGGATCGATCGCGAGCTGCGGCGTCGAGGCGGCCGCCGTCTCCGGCGAGCTCGTCACGGTGCTCTCGCCGGAGTTGAGGAGGCACCTCGTGGAGGAGGTGGTGCAAGTGCTCAAGAACGACGCCGAGTTCGTGGAGGCCGTGACCAACGCCGTGGCCGCTAGAGTTGTGGATCAAATCCCACACATTCCTGTTCATTTGTAGTTGTTTTTTTTTTCTCTTTGAGAACGTTGATTTGTAGTTTGTAGGCAGTATATATATCCCAAAATGCTTGCAAGTATATGTACACATGTATTCGCAAATGTGAGTATATACCTATGCCGCTTTTGCTATACGTCAGTTGCCTAATAGTTTTATTGCTTTTCTATTTAGCGATCCCCGAACACACTTTTTATTTGTTAGCGAGCTAAACTTATATAATGTATAAATTATTAAATATTGCAATACGT - >LOC_Os09g25070.2
AAAGTTCACGTTTTGGTCCCCCTCCTGTTCGGCGACGGGCGACGTACGGCGCCACTATAAGAAACGTGCCCTATTGCTGTCACGCCGTACTTCCTCCCGCCCACCGGGCAGCTAGCTTAGCTGCCGCCATGGACGACGACGGCGACGGCTCATCGTCACCGACCGACGACAGCGCCGCCGCCGGGCTTTTGCCGCTCTTCAGCCGATCGCCGGCCGAGGATCTTGAGGAGAAGCTGAGGCGGGCGATGGAGGAGAACGCGCGGCTGACGCGAGCGCTCGACGCCATTCTGGCCGGTCACCACGCCCACCAGCGCGCGCTCTTGGCGCCGTCGCTGTCGCCGCCGCCGCCTTCCGCCACAGCAAGGGCGCCGAGCGTGTCCACCAGCTGCGCGGCGAGGGAGGACGCCGCACCGGCGGTCGCCGCCGCCGCCGCGTCGACGGCATGTCCGTCGAGACAGCAGCCGCCGACCGCGGAGCCACGGCCCAAGGTCAGGACGGTCCGCGTGCGCGCCGACGCCGCCGACGCCACCGACGCCAACAGCATGGCGGAGACCGTGAAGGATGGGTACCAATGGAGGAAGTACGGGCAGAAGGTGACGCGTGACAACCCCTACCCTAGAGCTTACTTCCGCTGCGCATTCGCGCCGTCTTGCCCCGTCAAGAAGAAGCTCCAAAGATGTGCGGAGGATAGGTCAATGCTAGTGGCGACCTACGAGGGAGAACACAACCATGCCCTGTCCACGCAGACAACCGAATTCGTCGCCAGCGGCTGCACTACGAGCCAGCACGCCGGGGGGTCGTCGTCGTCGCCGCTGCCGTGCTCCATCTCCATCAACTCGTCGGGCCGCACGATCACACTCGACCTGACGAACCAAGCAGGGTCCGGATCGATCGCGAGCTGCGGCGTCGAGGCGGCCGCCGTCTCCGGCGAGCTCGTCACGGTGCTCTCGCCGGAGTTGAGGAGGCACCTCGTGGAGGAGGTGGTGCAAGTGCTCAAGAACGACGCCGAGTTCGTGGAGGCCGTGACCAACGCCGTGGCCGCTAGAGTTGTGGATCAAATCCCACACATTCCTGTTCATTTGTAGTTGTTTTTTTTTTCTCTTTGAGAACGTTGATTTGTAGTTTGTAGGCAGTATATATATCCCAAAATGCTTGCAAGTATATGTACACATGTATTCGCAAATGTGAGTATATACCTATGCCGCTTTTGCTATACGTCAGTTGCCTAATAGTTTTATTGCTTTTCTATTTAGCGATCCCCGAACACACTTTTTATTTGTTAGCGAGCTAAACTTATATAATGTATAAATTATTAAATATTGCAATACGT
CDS Sequence
- >LOC_Os09g25070.1
ATGGACGACGACGGCGACGGCTCATCGTCACCGACCGACGACAGCGCCGCCGCCGGGCTTTTGCCGCTCTTCAGCCGATCGCCGGCCGAGGATCTTGAGGAGAAGCTGAGGCGGGCGATGGAGGAGAACGCGCGGCTGACGCGAGCGCTCGACGCCATTCTGGCCGGTCACCACGCCCACCAGCGCGCGCTCTTGGCGCCGTCGCTGTCGCCGCCGCCGCCTTCCGCCACAGCAAGGGCGCCGAGCGTGTCCACCAGCTGCGCGGCGAGGGAGGACGCCGCACCGGCGGTCGCCGCCGCCGCCGCGTCGACGGCATGTCCGTCGAGACAGCAGCCGCCGACCGCGGAGCCACGGCCCAAGGTCAGGACGGTCCGCGTGCGCGCCGACGCCGCCGACGCCACCGACGCCAACAGCATGGCGGAGACCGTGAAGGATGGGTACCAATGGAGGAAGTACGGGCAGAAGGTGACGCGTGACAACCCCTACCCTAGAGCTTACTTCCGCTGCGCATTCGCGCCGTCTTGCCCCGTCAAGAAGAAGCTCCAAAGATGTGCGGAGGATAGGTCAATGCTAGTGGCGACCTACGAGGGAGAACACAACCATGCCCTGTCCACGCAGACAACCGAATTCGTCGCCAGCGGCTGCACTACGAGCCAGCACGCCGGGGGGTCGTCGTCGTCGCCGCTGCCGTGCTCCATCTCCATCAACTCGTCGGGCCGCACGATCACACTCGACCTGACGAACCAAGCAGGGTCCGGATCGATCGCGAGCTGCGGCGTCGAGGCGGCCGCCGTCTCCGGCGAGCTCGTCACGGTGCTCTCGCCGGAGTTGAGGAGGCACCTCGTGGAGGAGGTGGTGCAAGTGCTCAAGAACGACGCCGAGTTCGTGGAGGCCGTGACCAACGCCGTGGCCGCTAGAGTTGTGGATCAAATCCCACACATTCCTGTTCATTTGTAG - >LOC_Os09g25070.2
ATGGACGACGACGGCGACGGCTCATCGTCACCGACCGACGACAGCGCCGCCGCCGGGCTTTTGCCGCTCTTCAGCCGATCGCCGGCCGAGGATCTTGAGGAGAAGCTGAGGCGGGCGATGGAGGAGAACGCGCGGCTGACGCGAGCGCTCGACGCCATTCTGGCCGGTCACCACGCCCACCAGCGCGCGCTCTTGGCGCCGTCGCTGTCGCCGCCGCCGCCTTCCGCCACAGCAAGGGCGCCGAGCGTGTCCACCAGCTGCGCGGCGAGGGAGGACGCCGCACCGGCGGTCGCCGCCGCCGCCGCGTCGACGGCATGTCCGTCGAGACAGCAGCCGCCGACCGCGGAGCCACGGCCCAAGGTCAGGACGGTCCGCGTGCGCGCCGACGCCGCCGACGCCACCGACGCCAACAGCATGGCGGAGACCGTGAAGGATGGGTACCAATGGAGGAAGTACGGGCAGAAGGTGACGCGTGACAACCCCTACCCTAGAGCTTACTTCCGCTGCGCATTCGCGCCGTCTTGCCCCGTCAAGAAGAAGCTCCAAAGATGTGCGGAGGATAGGTCAATGCTAGTGGCGACCTACGAGGGAGAACACAACCATGCCCTGTCCACGCAGACAACCGAATTCGTCGCCAGCGGCTGCACTACGAGCCAGCACGCCGGGGGGTCGTCGTCGTCGCCGCTGCCGTGCTCCATCTCCATCAACTCGTCGGGCCGCACGATCACACTCGACCTGACGAACCAAGCAGGGTCCGGATCGATCGCGAGCTGCGGCGTCGAGGCGGCCGCCGTCTCCGGCGAGCTCGTCACGGTGCTCTCGCCGGAGTTGAGGAGGCACCTCGTGGAGGAGGTGGTGCAAGTGCTCAAGAACGACGCCGAGTTCGTGGAGGCCGTGACCAACGCCGTGGCCGCTAGAGTTGTGGATCAAATCCCACACATTCCTGTTCATTTGTAG
Protein Sequence
- >LOC_Os09g25070.1
MDDDGDGSSSPTDDSAAAGLLPLFSRSPAEDLEEKLRRAMEENARLTRALDAILAGHHAHQRALLAPSLSPPPPSATARAPSVSTSCAAREDAAPAVAAAAASTACPSRQQPPTAEPRPKVRTVRVRADAADATDANSMAETVKDGYQWRKYGQKVTRDNPYPRAYFRCAFAPSCPVKKKLQRCAEDRSMLVATYEGEHNHALSTQTTEFVASGCTTSQHAGGSSSSPLPCSISINSSGRTITLDLTNQAGSGSIASCGVEAAAVSGELVTVLSPELRRHLVEEVVQVLKNDAEFVEAVTNAVAARVVDQIPHIPVHL* - >LOC_Os09g25070.2
MDDDGDGSSSPTDDSAAAGLLPLFSRSPAEDLEEKLRRAMEENARLTRALDAILAGHHAHQRALLAPSLSPPPPSATARAPSVSTSCAAREDAAPAVAAAAASTACPSRQQPPTAEPRPKVRTVRVRADAADATDANSMAETVKDGYQWRKYGQKVTRDNPYPRAYFRCAFAPSCPVKKKLQRCAEDRSMLVATYEGEHNHALSTQTTEFVASGCTTSQHAGGSSSSPLPCSISINSSGRTITLDLTNQAGSGSIASCGVEAAAVSGELVTVLSPELRRHLVEEVVQVLKNDAEFVEAVTNAVAARVVDQIPHIPVHL*