Information report for YLC1;OsV5A
Gene Details
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Functional Descriptions
- These data suggested that the YLC1 protein may be involved in Chl and lutein accumulation and chloroplast development at early leaf development in rice.
- Real-time PCR analyses showed that the expression levels of the genes associated with Chl biosynthesis and photosynthesis were affected in YLC1 mutant at different temperatures.
- In this study, we characterized a rice mutant named young leaf chlorosis 1 (YLC1) from a (6)(0)Co-irradiated population.
- The Young Leaf Chlorosis 1 (YLC1) gene was isolated via map-based cloning and identified to encode a protein of unknown function belonging to the DUF3353 superfamily.
- In rice protoplasts, the YLC1 protein displayed a typical chloroplast location pattern.
- Differential protein abundances of OsV5A and OsV5B in rice seedlings at different leaf developmental stages were also revealed.
- Deficiency of OsV5A and OsV5B occurred in pre-emerged and emerging leaves in OsV5A seedlings at 22 °C, leading to reduced POR accumulation and chlorophyll content.
- OsV5A apparently developed a specialized role for regulating POR abundances at the leaf pre-emergence stage while the same function is performed by OsV5B during leaf emergence and expansion.
- Both OsV5A and OsV5B are localized in the chloroplast envelope and thylakoid membranes.
- OsV5A and OsV5B interact with two rice PORs (OsPORA and OsPORB) inside chloroplasts and they stabilize OsPORB in vitro under oxidative stress.
- Map-based cloning revealed that OsV5A is a J-like protein with four transmembrane domains.
Functional Keywords
Literature and News
- Young Leaf Chlorosis 1, a chloroplast-localized gene required for chlorophyll and lutein accumulation during early leaf development in rice . DOI: 10.1007/s00425-012-1756-1 ; PMID: 23053539
- Differential Regulation of Protochlorophyllide Oxidoreductase Abundances by VIRESCENT 5A (OsV5A) and VIRESCENT 5B (OsV5B) in Rice Seedlings . DOI: 10.1093/pcp/pcw151 ; PMID: 27565208
Gene Resources
- UniProt: A3BYC5
- EMBL: CM000146
- AlphaFoldDB: A3BYC5
- EnsemblPlants: Os09t0380200-01
- Gramene: Os09t0380200-01
- KEGG: osa:4346927
- InterPro: IPR021788
- PANTHER: PTHR33372, PTHR33372:SF7
- OrthoDB: A3BYC5
- SWISS-MODEL: A3BYC5
Sequences
cDNA Sequence
- >LOC_Os09g21250.1
ATGCAGGCGACGGCCGCGCTCTTCCTCGTCCGCCCGCTCCCGCGTCCCCACTACCGGTGCTTGCATGGGTTGCGGGGAGGCGTCTCGCTGGCCCCGCCGCGGCGGCGGCTGGTGGCGCGGGGCCCGCGGTGCTCCATGAGCCTCTCCATCGGCGGCGGCGCGGGCGCGGGCGCCGGCGGGGACCGCGGGTTCAGCTACGAGCACGTTCCAGTGTTTCCAAGATACCGGATTCGTGATCCCTACAAGCTTCTTGGTGTTGATCGTGATGCAGCTGAAGAAGAGATCAGGAGTGCTAGAAATTTCCTTATTCAACAGTATGCTGGGCATGAACCAAGTGAAGAAGCTATTGAAGGTGCATACGAGAAGATAATAATGAAGAGCTACCAGCAGCGGAAAAAGACTAAGATTAATCTGAAAACTAAGCTAAAAAAGCGAGTTGAGGAATCCCCGTCATGGGTAAAGGCACTGCTTGGATATTTTGAGGTGCCACAAATGGATATCATTTCAAGAAGATTGTTCTTTTTCGCTTTCATTGCTGGTTGGAGCATAGCGACTTCTGCTGAGAATGGACCTGCATTCCAGCTTGCAATATCTCTCTTCTCGTGCATATATTTCCTTAATGATAAGATGAAGAACCTCATGAGGGCATCAACAACTGGGTTTGGAGTGCTTGTTGGTGGCTGGATAATTGGCTCTCTATTGGTCCCACTTATCCCAACATTCATCATCCCACCCTCTTGGTCCCTTGAGCTACTTACATCATTAGTTGCATATGTCTTCCTGTTCCTGGGTTGCACTTTCCTCAAATGAAAACCTTGTAAATCTTGTAACCGTAAATTGTAGAATTGCTTGCAGAAATTTTGCAACTACTGACATCTCCACTATGGGTCTCGGAAAAGTTTTGTAGAATGACATCACTTTGAGGTTCTCCTCTTCTTCCATATGGTCAAGCCTCAGAATTTTCTGTTCAGCACTTCTCTGAAACAAGAAAATCAATCTGTCCACAACTTCAGGTCATTTTACTCTATCGTTTAGCCTTTTTTCTTTTTGGAACACATTAATCTAATATAACCCGATTGATTATT
CDS Sequence
- >LOC_Os09g21250.1
ATGCAGGCGACGGCCGCGCTCTTCCTCGTCCGCCCGCTCCCGCGTCCCCACTACCGGTGCTTGCATGGGTTGCGGGGAGGCGTCTCGCTGGCCCCGCCGCGGCGGCGGCTGGTGGCGCGGGGCCCGCGGTGCTCCATGAGCCTCTCCATCGGCGGCGGCGCGGGCGCGGGCGCCGGCGGGGACCGCGGGTTCAGCTACGAGCACGTTCCAGTGTTTCCAAGATACCGGATTCGTGATCCCTACAAGCTTCTTGGTGTTGATCGTGATGCAGCTGAAGAAGAGATCAGGAGTGCTAGAAATTTCCTTATTCAACAGTATGCTGGGCATGAACCAAGTGAAGAAGCTATTGAAGGTGCATACGAGAAGATAATAATGAAGAGCTACCAGCAGCGGAAAAAGACTAAGATTAATCTGAAAACTAAGCTAAAAAAGCGAGTTGAGGAATCCCCGTCATGGGTAAAGGCACTGCTTGGATATTTTGAGGTGCCACAAATGGATATCATTTCAAGAAGATTGTTCTTTTTCGCTTTCATTGCTGGTTGGAGCATAGCGACTTCTGCTGAGAATGGACCTGCATTCCAGCTTGCAATATCTCTCTTCTCGTGCATATATTTCCTTAATGATAAGATGAAGAACCTCATGAGGGCATCAACAACTGGGTTTGGAGTGCTTGTTGGTGGCTGGATAATTGGCTCTCTATTGGTCCCACTTATCCCAACATTCATCATCCCACCCTCTTGGTCCCTTGAGCTACTTACATCATTAGTTGCATATGTCTTCCTGTTCCTGGGTTGCACTTTCCTCAAATGA
Protein Sequence
- >LOC_Os09g21250.1
MQATAALFLVRPLPRPHYRCLHGLRGGVSLAPPRRRLVARGPRCSMSLSIGGGAGAGAGGDRGFSYEHVPVFPRYRIRDPYKLLGVDRDAAEEEIRSARNFLIQQYAGHEPSEEAIEGAYEKIIMKSYQQRKKTKINLKTKLKKRVEESPSWVKALLGYFEVPQMDIISRRLFFFAFIAGWSIATSAENGPAFQLAISLFSCIYFLNDKMKNLMRASTTGFGVLVGGWIIGSLLVPLIPTFIIPPSWSLELLTSLVAYVFLFLGCTFLK*