Gene Details:
- MSU gene ID: LOC_Os09g21250
- RAPdb gene ID: Os09g0380200
- Gene Symbol: YLC1 OsV5A
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- These data suggested that the YLC1 protein may be involved in Chl and lutein accumulation and chloroplast development at early leaf development in rice.
- Real-time PCR analyses showed that the expression levels of the genes associated with Chl biosynthesis and photosynthesis were affected in YLC1 mutant at different temperatures.
- In this study, we characterized a rice mutant named young leaf chlorosis 1 (YLC1) from a (6)(0)Co-irradiated population.
- The Young Leaf Chlorosis 1 (YLC1) gene was isolated via map-based cloning and identified to encode a protein of unknown function belonging to the DUF3353 superfamily.
- In rice protoplasts, the YLC1 protein displayed a typical chloroplast location pattern.
- Differential protein abundances of OsV5A and OsV5B in rice seedlings at different leaf developmental stages were also revealed.
- Deficiency of OsV5A and OsV5B occurred in pre-emerged and emerging leaves in OsV5A seedlings at 22 °C, leading to reduced POR accumulation and chlorophyll content.
- OsV5A apparently developed a specialized role for regulating POR abundances at the leaf pre-emergence stage while the same function is performed by OsV5B during leaf emergence and expansion.
- Both OsV5A and OsV5B are localized in the chloroplast envelope and thylakoid membranes.
- OsV5A and OsV5B interact with two rice PORs (OsPORA and OsPORB) inside chloroplasts and they stabilize OsPORB in vitro under oxidative stress.
- Map-based cloning revealed that OsV5A is a J-like protein with four transmembrane domains.
Function-related keywords:
- leaf-development , photosynthesis , leaf , chloroplast , temperature , seedlings , oxidative-stress , map-based-cloning , oxidative , stress
Literature:
- Young Leaf Chlorosis 1, a chloroplast-localized gene required for chlorophyll and lutein accumulation during early leaf development in rice . DOI: 10.1007/s00425-012-1756-1 ; PMID: 23053539
- Differential Regulation of Protochlorophyllide Oxidoreductase Abundances by VIRESCENT 5A (OsV5A) and VIRESCENT 5B (OsV5B) in Rice Seedlings . DOI: 10.1093/pcp/pcw151 ; PMID: 27565208
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os09g21250.1
ATGCAGGCGACGGCCGCGCTCTTCCTCGTCCGCCCGCTCCCGCGTCCCCACTACCGGTGCTTGCATGGGTTGCGGGGAGGCGTCTCGCTGGCCCCGCCGCGGCGGCGGCTGGTGGCGCGGGGCCCGCGGTGCTCCATGAGCCTCTCCATCGGCGGCGGCGCGGGCGCGGGCGCCGGCGGGGACCGCGGGTTCAGCTACGAGCACGTTCCAGTGTTTCCAAGATACCGGATTCGTGATCCCTACAAGCTTCTTGGTGTTGATCGTGATGCAGCTGAAGAAGAGATCAGGAGTGCTAGAAATTTCCTTATTCAACAGTATGCTGGGCATGAACCAAGTGAAGAAGCTATTGAAGGTGCATACGAGAAGATAATAATGAAGAGCTACCAGCAGCGGAAAAAGACTAAGATTAATCTGAAAACTAAGCTAAAAAAGCGAGTTGAGGAATCCCCGTCATGGGTAAAGGCACTGCTTGGATATTTTGAGGTGCCACAAATGGATATCATTTCAAGAAGATTGTTCTTTTTCGCTTTCATTGCTGGTTGGAGCATAGCGACTTCTGCTGAGAATGGACCTGCATTCCAGCTTGCAATATCTCTCTTCTCGTGCATATATTTCCTTAATGATAAGATGAAGAACCTCATGAGGGCATCAACAACTGGGTTTGGAGTGCTTGTTGGTGGCTGGATAATTGGCTCTCTATTGGTCCCACTTATCCCAACATTCATCATCCCACCCTCTTGGTCCCTTGAGCTACTTACATCATTAGTTGCATATGTCTTCCTGTTCCTGGGTTGCACTTTCCTCAAATGAAAACCTTGTAAATCTTGTAACCGTAAATTGTAGAATTGCTTGCAGAAATTTTGCAACTACTGACATCTCCACTATGGGTCTCGGAAAAGTTTTGTAGAATGACATCACTTTGAGGTTCTCCTCTTCTTCCATATGGTCAAGCCTCAGAATTTTCTGTTCAGCACTTCTCTGAAACAAGAAAATCAATCTGTCCACAACTTCAGGTCATTTTACTCTATCGTTTAGCCTTTTTTCTTTTTGGAACACATTAATCTAATATAACCCGATTGATTATT
CDS Sequence
- >LOC_Os09g21250.1
ATGCAGGCGACGGCCGCGCTCTTCCTCGTCCGCCCGCTCCCGCGTCCCCACTACCGGTGCTTGCATGGGTTGCGGGGAGGCGTCTCGCTGGCCCCGCCGCGGCGGCGGCTGGTGGCGCGGGGCCCGCGGTGCTCCATGAGCCTCTCCATCGGCGGCGGCGCGGGCGCGGGCGCCGGCGGGGACCGCGGGTTCAGCTACGAGCACGTTCCAGTGTTTCCAAGATACCGGATTCGTGATCCCTACAAGCTTCTTGGTGTTGATCGTGATGCAGCTGAAGAAGAGATCAGGAGTGCTAGAAATTTCCTTATTCAACAGTATGCTGGGCATGAACCAAGTGAAGAAGCTATTGAAGGTGCATACGAGAAGATAATAATGAAGAGCTACCAGCAGCGGAAAAAGACTAAGATTAATCTGAAAACTAAGCTAAAAAAGCGAGTTGAGGAATCCCCGTCATGGGTAAAGGCACTGCTTGGATATTTTGAGGTGCCACAAATGGATATCATTTCAAGAAGATTGTTCTTTTTCGCTTTCATTGCTGGTTGGAGCATAGCGACTTCTGCTGAGAATGGACCTGCATTCCAGCTTGCAATATCTCTCTTCTCGTGCATATATTTCCTTAATGATAAGATGAAGAACCTCATGAGGGCATCAACAACTGGGTTTGGAGTGCTTGTTGGTGGCTGGATAATTGGCTCTCTATTGGTCCCACTTATCCCAACATTCATCATCCCACCCTCTTGGTCCCTTGAGCTACTTACATCATTAGTTGCATATGTCTTCCTGTTCCTGGGTTGCACTTTCCTCAAATGA
Protein Sequence
- >LOC_Os09g21250.1
MQATAALFLVRPLPRPHYRCLHGLRGGVSLAPPRRRLVARGPRCSMSLSIGGGAGAGAGGDRGFSYEHVPVFPRYRIRDPYKLLGVDRDAAEEEIRSARNFLIQQYAGHEPSEEAIEGAYEKIIMKSYQQRKKTKINLKTKLKKRVEESPSWVKALLGYFEVPQMDIISRRLFFFAFIAGWSIATSAENGPAFQLAISLFSCIYFLNDKMKNLMRASTTGFGVLVGGWIIGSLLVPLIPTFIIPPSWSLELLTSLVAYVFLFLGCTFLK*