Gene Details:
- MSU gene ID: LOC_Os09g11450
- RAPdb gene ID: Os09g0286400
- Gene Symbol: OsNHX5
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- OsNHX1 promoter-GUS activity only was localized to the guard cells and trichome, whereas OsNHX5 promoter-GUS activity only was localized to the root tip and pollen grains.
- When we studied the expression of beta-glucuronidase (GUS) driven by either the OsNHX1 or the OsNHX5 promoter, we observed activity in the stele, the emerging part of lateral roots, the vascular bundle, the water pore, and the basal part of seedling shoots with both promoters.
- The expression of OsNHX1, OsNHX2, OsNHX3, and OsNHX5 is regulated differently in rice tissues and is increased by salt stress, hyperosmotic stress, and ABA.
- The OsNHX5 protein colocalizes to the Golgi, the trans-Golgi network (TGN) and the pre-vacuolar compartment (PVC) in tobacco leaf epidermal cells.
- Our results demonstrated an important role of OsNHX5 in regulating endomembrane luminal pH, which is essential for seed storage protein trafficking in rice.
Function-related keywords:
- pollen , seedling , grain , vascular-bundle , lateral-root , salt , shoot , salt-stress , root , leaf , seed
Literature:
- Molecular and functional analyses of rice NHX-type Na+/H+ antiporter genes . DOI: 10.1007/s00425-010-1289-4 ; PMID: 20963607
- OsNHX5-mediated pH homeostasis is required for post-Golgi trafficking of seed storage proteins in rice endosperm cells . DOI: 10.1186/s12870-019-1911-y ; PMID: 31277576
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os09g11450.1
ATGGCGCTGGAGCTGAGCCTGGTGTCCGCTTCGCCCCCTGGTGGGCTGGCGGCGAGCCCGCCCCCGCCCGCCGCCATCCCGGGGAAGGAGCAGCAGGTGGCCGGGGTGGGGATCCTGCTGCAGATCTCCATGCTCGTGCTCTCCTTCGTGCTCGGCCACGTCCTCCGCCGCCACCGGTTCTACTACCTCCCCGAGGCCAGCGCGTCTCTCCTCATCGGTCTAGTTGTCGGTGGGCTTGCTAATATTTCAAATACAGAGACCAACACTAGAACCCAATCAGGATTCAGTCTATCCCCAAAACCATTCTTTGCAAATTTTGGGGCTATTGTAACTTTTGCCATCCTTGGGACATTCATTGCTTCTGTTGTAACAGGAGTTCTCGTCTATCTTGGTGGGTTGACATTTCTAATGTACAAACTTCCATTTGTTGAGTGCCTCATGTTCGGTGCTCTTATATCTGCAACTGATCCTGTCACTGTGTTATCAATATTCCAGGAGCTGGGCACTGATGTTAACTTGTATGCTCTTGTGTTTGGAGAATCTGTTCTAAATGATGCGATGGCGATTTCGCTTTACAGGACAATGTCATTGGTCAGAAGTCAAGCAGCAGCTGGGGAGAACTTCTTTATGATGGTTTTCCAGTTCCTTGAGACCTTTGTTGGTTCATTGTCATCAGGTTGCATCACAGCTCAGAATATTCTCAACTATTTCAAAGGGACCTTTGGCATTGTGGTGTTGGAGTTGGATTTATCTCTGCTCTTATATCCTTTTCCTAAGTTCTACATGGAAACTCTGTTTGCAAAACATGAAAGACTATGCATGCTTAGTATTGACCATGTTAAATTGCAATATTTATGTATTACTATTTACCATTTACTCATTGCTTGTTTTACGGTAAATTATTTTATTTACTACTTGATCTTGTGCAACCTTGACCACTCTGTGTACCTGTTTAAGTATGCTGGACTGGATATTGACAAGTATATGTTAGCAGAAGGACTTGGGTTGTCAGGAATTGTTTCCATACTATTCACAGGAATGGTTATGAAGCACTATACATTTTCCAATCTCTCAAACAACTCTCAGCGCTTCGTTTCTGCCTTCTTTCACTTGCTGTCATCTTTAGCAGAAACATTTGTGGCAGTAAATGTCTTTTCTTGTGCATATTTGGTTAACATGTCACGGCCAGAACATCGACGCATACCTCTAAAGCATCAGAAAGCACTTTGGTTTAGTGGGCTTAGAGGGGCCATGGCTTTTGCACTTGCTCTCCAATCTGCCAATGAACTTCCTGGAGGACATGGAAAAACAATATTCACAACCACCACAGCTATTGTTGTTTTAACGGTACTTCTTATCGGAGGATCGACGGGCACCATGCTGGAAGCTTTGGATGTAATTGGTGATGAAAACAGATCAATAGAAAATTATGACGACAACAATGGTTACATCCCCCCAACGTATGAGGAAGGTTCATCATCTGGAGGAGGATTAAGAATGAAACTGAAGGAATTCCACAAAAGCACGACATCGTTCACTGCCCTTGACAGGAACTATCTGACTCCATTTTTCACCAGTCAAACTGATGAGGATGATGATGTCTTCGGTGAACAACCCCAAAACCAGAGACGCGGATTCTATGACCAATAGCTTGGATGAGTTGCCACCTGACAGCCACATACACGATACTTAGCATGGTAGCATGTGATGAGATTGGAAGCGATCACCAGTACTCCGCAACACCTCCTCCGGCTGTGCCGCCGCCGCCGCCTGCGCGGACCAGTACTCCGCCGCGACGCCCGATAACTCCTCCGTCACGTTCGGTGACGACGAGGCCGACAACGAGTCGCATAGCAGCGAAGGCTACGAGCCCGAAGCCAAGTGCTGGAAGGAGGATGCTGACAATGAGGGTAGCTCCGGCGGCATGGGCGGCGGCGCTGGCGGGAAGCCGGTGCGCAAGCCGAGGCTTGTGGTGCACACGCTGAGCGACATCGACGTCAACATCGACATCCTCGACGCCGGCTTCCGGTGGAGGAAGTACGGCCAGAAGGTCGTCAAGGGCAACCCCAACCCAAGGAGCTACTACAAGTGCACGACGGTGGGTTGCCCGGTGCGGTAGCACGTGGAGCGGGCGTTGCACGACACGCGTGCCGTGATCACCACATACGCCGGCGCCGTCGTCCAGCGCGATCCGGCCGTCGGCTGTCTCCGGCCAGCAGGGGCACTACACCCTCGAG - >LOC_Os09g11450.2
ATGGCGCTGGAGCTGAGCCTGGTGTCCGCTTCGCCCCCTGGTGGGCTGGCGGCGAGCCCGCCCCCGCCCGCCGCCATCCCGGGGAAGGAGCAGCAGGTGGCCGGGGTGGGGATCCTGCTGCAGATCTCCATGCTCGTGCTCTCCTTCGTGCTCGGCCACGTCCTCCGCCGCCACCGGTTCTACTACCTCCCCGAGGCCAGCGCGTCTCTCCTCATCGGTCTAGTTGTCGGTGGGCTTGCTAATATTTCAAATACAGAGACCAACACTAGAACCCAATCAGGATTCAGTCTATCCCCAAAACCATTCTTTGCAAATTTTGGGGCTATTGTAACTTTTGCCATCCTTGGGACATTCATTGCTTCTGTTGTAACAGGAGTTCTCGTCTATCTTGGTGGGTTGACATTTCTAATGTACAAACTTCCATTTGTTGAGTGCCTCATGTTCGGTGCTCTTATATCTGCAACTGATCCTGTCACTGTGTTATCAATATTCCAGGAGCTGGGCACTGATGTTAACTTGTATGCTCTTGTGTTTGGAGAATCTGTTCTAAATGATGCGATGGCGATTTCGCTTTACAGGACAATGTCATTGGTCAGAAGTCAAGCAGCAGCTGGGGAGAACTTCTTTATGATGGTTTTCCAGTTCCTTGAGACCTTTGTTGGTTCATTGTCATCAGGTTGCATCACAGCTCAGAATATTCTCAACTATTTCAAAGGGACCTTTGGCATTGTGGTGTTGGAGTTGGATTTATCTCTGCTCTTATATCCTTTTCCTAAGTTCTACATGGAAACTCTGTTTGCAAAACATGAAAGACTATGCATGCTTAGTATTGACCATGTTAAATTGCAATATTTATGTATTACTATTTACCATTTACTCATTGCTTGTTTTACGGTAAATTATTTTATTTACTACTTGATCTTGTGCAACCTTGACCACTCTGTGTACCTGTTTAAGTATGCTGGACTGGATATTGACAAGTATATGTTAGCAGAAGGACTTGGGTTGTCAGGAATTGTTTCCATACTATTCACAGGAATGGTTATGAAGCACTATACATTTTCCAATCTCTCAAACAACTCTCAGCGCTTCGTTTCTGCCTTCTTTCACTTGCTGTCATCTTTAGCAGAAACATTTGTGGCAGTAAATGTCTTTTCTTGTGCATATTTGGTTAACATGTCACGGCCAGAACATCGACGCATACCTCTAAAGCATCAGAAAGCACTTTGGTTTAGTGGGCTTAGAGGGGCCATGGCTTTTGCACTTGCTCTCCAATCTGCCAATGAACTTCCTGGAGGACATGGAAAAACAATATTCACAACCACCACAGCTATTGTTGTTTTAACGGTACTTCTTATCGGAGGATCGACGGGCACCATGCTGGAAGCTTTGGATGTAATTGGTGATGAAAACAGATCAATAGAAAATTATGACGACAACAATGGTTACATCCCCCCAACGTATGAGGAAGGTTCATCATCTGGAGGAGGATTAAGAATGAAACTGAAGGAATTCCACAAAAGCACGACATCGTTCACTGCCCTTGACAGGAACTATCTGACTCCATTTTTCACCAGTCAAACTGATGAGGATGATGATGTCTTCGGTGAACAACCCCAAAACCAGAGACGCGGATTCTATGACCAATAGCTTGGATGAGTTGCCACCTGACAGCCACATACACGATACTTAGCATGGTAGCATGTGATGAGATTGGAAGCGATCACCAGTACTCCGCAACACCTCCTCCGGCTGTGCCGCCGCCGCCGCCTGCGCGGACCAGTACTCCGCCGCGACGCCCGATAACTCCTCCGTCACGTTCGGTGACGACGAGGCCGACAACGAGTCGCATAGCAGCGAAGGCTACGAGCCCGAAGCCAAGTGCTGGAAGGAGGATGCTGACAATGAGGGTAGCTCCGGCGGCATGGGCGGCGGCGCTGGCGGGAAGCCGGTGCGCAAGCCGAGGCTTGTGGTGCACACGCTGAGCGACATCGACGTCAACATCGACATCCTCGACGCCGGCTTCCGGTGGAGGAAGTACGGCCAGAAGGTCGTCAAGGGCAACCCCAACCCAAGGAGCTACTACAAGTGCACGACGGTGGGTTGCCCGGTGCGGTAGCACGTGGAGCGGGCGTTGCACGACACGCGTGCCGTGATCACCACATACGCCGGCGCCGTCGTCCAGCGCGATCCGGCCGTCGGCTGTCTCCGGCCAGCAGGGGCACTACACCCTCGAG
CDS Sequence
- >LOC_Os09g11450.1
ATGGCGCTGGAGCTGAGCCTGGTGTCCGCTTCGCCCCCTGGTGGGCTGGCGGCGAGCCCGCCCCCGCCCGCCGCCATCCCGGGGAAGGAGCAGCAGGTGGCCGGGGTGGGGATCCTGCTGCAGATCTCCATGCTCGTGCTCTCCTTCGTGCTCGGCCACGTCCTCCGCCGCCACCGGTTCTACTACCTCCCCGAGGCCAGCGCGTCTCTCCTCATCGGTCTAGTTGTCGGTGGGCTTGCTAATATTTCAAATACAGAGACCAACACTAGAACCCAATCAGGATTCAGTCTATCCCCAAAACCATTCTTTGCAAATTTTGGGGCTATTGTAACTTTTGCCATCCTTGGGACATTCATTGCTTCTGTTGTAACAGGAGTTCTCGTCTATCTTGGTGGGTTGACATTTCTAATGTACAAACTTCCATTTGTTGAGTGCCTCATGTTCGGTGCTCTTATATCTGCAACTGATCCTGTCACTGTGTTATCAATATTCCAGGAGCTGGGCACTGATGTTAACTTGTATGCTCTTGTGTTTGGAGAATCTGTTCTAAATGATGCGATGGCGATTTCGCTTTACAGGACAATGTCATTGGTCAGAAGTCAAGCAGCAGCTGGGGAGAACTTCTTTATGATGGTTTTCCAGTTCCTTGAGACCTTTGTTGGTTCATTGTCATCAGGTTGCATCACAGCTCAGAATATTCTCAACTATTTCAAAGGGACCTTTGGCATTGTGGTGTTGGAGTTGGATTTATCTCTGCTCTTATATCCTTTTCCTAAGTTCTACATGGAAACTCTGTTTGCAAAACATGAAAGACTATGCATGCTTAGTATTGACCATGTTAAATTGCAATATTTATGTATTACTATTTACCATTTACTCATTGCTTGTTTTACGGTAAATTATTTTATTTACTACTTGATCTTGTGCAACCTTGACCACTCTGTGTACCTGTTTAAGTATGCTGGACTGGATATTGACAAGTATATGTTAGCAGAAGGACTTGGGTTGTCAGGAATTGTTTCCATACTATTCACAGGAATGGTTATGAAGCACTATACATTTTCCAATCTCTCAAACAACTCTCAGCGCTTCGTTTCTGCCTTCTTTCACTTGCTGTCATCTTTAGCAGAAACATTTGTGGCAGTAAATGTCTTTTCTTGTGCATATTTGGTTAACATGTCACGGCCAGAACATCGACGCATACCTCTAAAGCATCAGAAAGCACTTTGGTTTAGTGGGCTTAGAGGGGCCATGGCTTTTGCACTTGCTCTCCAATCTGCCAATGAACTTCCTGGAGGACATGGAAAAACAATATTCACAACCACCACAGCTATTGTTGTTTTAACGGTACTTCTTATCGGAGGATCGACGGGCACCATGCTGGAAGCTTTGGATGTAATTGGTGATGAAAACAGATCAATAGAAAATTATGACGACAACAATGGTTACATCCCCCCAACGTATGAGGAAGGTTCATCATCTGGAGGAGGATTAAGAATGAAACTGAAGGAATTCCACAAAAGCACGACATCGTTCACTGCCCTTGACAGGAACTATCTGACTCCATTTTTCACCAGTCAAACTGATGAGGATGATGATGTCTTCGGTGAACAACCCCAAAACCAGAGACGCGGATTCTATGACCAATAG - >LOC_Os09g11450.2
ATGGCGCTGGAGCTGAGCCTGGTGTCCGCTTCGCCCCCTGGTGGGCTGGCGGCGAGCCCGCCCCCGCCCGCCGCCATCCCGGGGAAGGAGCAGCAGGTGGCCGGGGTGGGGATCCTGCTGCAGATCTCCATGCTCGTGCTCTCCTTCGTGCTCGGCCACGTCCTCCGCCGCCACCGGTTCTACTACCTCCCCGAGGCCAGCGCGTCTCTCCTCATCGGTCTAGTTGTCGGTGGGCTTGCTAATATTTCAAATACAGAGACCAACACTAGAACCCAATCAGGATTCAGTCTATCCCCAAAACCATTCTTTGCAAATTTTGGGGCTATTGTAACTTTTGCCATCCTTGGGACATTCATTGCTTCTGTTGTAACAGGAGTTCTCGTCTATCTTGGTGGGTTGACATTTCTAATGTACAAACTTCCATTTGTTGAGTGCCTCATGTTCGGTGCTCTTATATCTGCAACTGATCCTGTCACTGTGTTATCAATATTCCAGGAGCTGGGCACTGATGTTAACTTGTATGCTCTTGTGTTTGGAGAATCTGTTCTAAATGATGCGATGGCGATTTCGCTTTACAGGACAATGTCATTGGTCAGAAGTCAAGCAGCAGCTGGGGAGAACTTCTTTATGATGGTTTTCCAGTTCCTTGAGACCTTTGTTGGTTCATTGTCATCAGGTTGCATCACAGCTCAGAATATTCTCAACTATTTCAAAGGGACCTTTGGCATTGTGGTGTTGGAGTTGGATTTATCTCTGCTCTTATATCCTTTTCCTAAGTTCTACATGGAAACTCTGTTTGCAAAACATGAAAGACTATGCATGCTTAGTATTGACCATGTTAAATTGCAATATTTATGTATTACTATTTACCATTTACTCATTGCTTGTTTTACGGTAAATTATTTTATTTACTACTTGATCTTGTGCAACCTTGACCACTCTGTGTACCTGTTTAAGTATGCTGGACTGGATATTGACAAGTATATGTTAGCAGAAGGACTTGGGTTGTCAGGAATTGTTTCCATACTATTCACAGGAATGGTTATGAAGCACTATACATTTTCCAATCTCTCAAACAACTCTCAGCGCTTCGTTTCTGCCTTCTTTCACTTGCTGTCATCTTTAGCAGAAACATTTGTGGCAGTAAATGTCTTTTCTTGTGCATATTTGGTTAACATGTCACGGCCAGAACATCGACGCATACCTCTAAAGCATCAGAAAGCACTTTGGTTTAGTGGGCTTAGAGGGGCCATGGCTTTTGCACTTGCTCTCCAATCTGCCAATGAACTTCCTGGAGGACATGGAAAAACAATATTCACAACCACCACAGCTATTGTTGTTTTAACGGTACTTCTTATCGGAGGATCGACGGGCACCATGCTGGAAGCTTTGGATGTAATTGGTGATGAAAACAGATCAATAGAAAATTATGACGACAACAATGGTTACATCCCCCCAACGTATGAGGAAGGTTCATCATCTGGAGGAGGATTAAGAATGAAACTGAAGGAATTCCACAAAAGCACGACATCGTTCACTGCCCTTGACAGGAACTATCTGACTCCATTTTTCACCAGTCAAACTGATGAGGATGATGATGTCTTCGGTGAACAACCCCAAAACCAGAGACGCGGATTCTATGACCAATAG
Protein Sequence
- >LOC_Os09g11450.1
MALELSLVSASPPGGLAASPPPPAAIPGKEQQVAGVGILLQISMLVLSFVLGHVLRRHRFYYLPEASASLLIGLVVGGLANISNTETNTRTQSGFSLSPKPFFANFGAIVTFAILGTFIASVVTGVLVYLGGLTFLMYKLPFVECLMFGALISATDPVTVLSIFQELGTDVNLYALVFGESVLNDAMAISLYRTMSLVRSQAAAGENFFMMVFQFLETFVGSLSSGCITAQNILNYFKGTFGIVVLELDLSLLLYPFPKFYMETLFAKHERLCMLSIDHVKLQYLCITIYHLLIACFTVNYFIYYLILCNLDHSVYLFKYAGLDIDKYMLAEGLGLSGIVSILFTGMVMKHYTFSNLSNNSQRFVSAFFHLLSSLAETFVAVNVFSCAYLVNMSRPEHRRIPLKHQKALWFSGLRGAMAFALALQSANELPGGHGKTIFTTTTAIVVLTVLLIGGSTGTMLEALDVIGDENRSIENYDDNNGYIPPTYEEGSSSGGGLRMKLKEFHKSTTSFTALDRNYLTPFFTSQTDEDDDVFGEQPQNQRRGFYDQ* - >LOC_Os09g11450.2
MALELSLVSASPPGGLAASPPPPAAIPGKEQQVAGVGILLQISMLVLSFVLGHVLRRHRFYYLPEASASLLIGLVVGGLANISNTETNTRTQSGFSLSPKPFFANFGAIVTFAILGTFIASVVTGVLVYLGGLTFLMYKLPFVECLMFGALISATDPVTVLSIFQELGTDVNLYALVFGESVLNDAMAISLYRTMSLVRSQAAAGENFFMMVFQFLETFVGSLSSGCITAQNILNYFKGTFGIVVLELDLSLLLYPFPKFYMETLFAKHERLCMLSIDHVKLQYLCITIYHLLIACFTVNYFIYYLILCNLDHSVYLFKYAGLDIDKYMLAEGLGLSGIVSILFTGMVMKHYTFSNLSNNSQRFVSAFFHLLSSLAETFVAVNVFSCAYLVNMSRPEHRRIPLKHQKALWFSGLRGAMAFALALQSANELPGGHGKTIFTTTTAIVVLTVLLIGGSTGTMLEALDVIGDENRSIENYDDNNGYIPPTYEEGSSSGGGLRMKLKEFHKSTTSFTALDRNYLTPFFTSQTDEDDDVFGEQPQNQRRGFYDQ*