Gene Details:

Functional Descriptions:

  • Taken together, our results indicated that OsMFS1 is indispensable to the normal development of anther and embryo sacs in rice.
  • Here, we characterized a completely sterile OsMFS1 (male and female sterility 1) mutant which has its pollen and embryo sacs both aborted at the reproductive stage due to severe chromosome defection.
  • Map-based cloning revealed that the OsMFS1 encodes a meiotic coiled-coil protein, and it is responsible for DSB repairing that acts as an important cofactor to stimulate the single strand invasion.
  • Subcellular localization analysis of OsMFS1 revealed its association with the nucleus exclusively.
  • Expression pattern analyses showed the OsMFS1 was preferentially expressed in meiosis stage.
  • In addition, a yeast two-hybrid (Y2H) and pull-down assay showed that OsMFS1 could physically interact with OsHOP2 protein to form a stable complex to ensure faithful homologous recombination.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os09g10850.1
    ATGTCGAAGAAGAGGGGTCTTTCTTTGGAGGAGAAGAGGGAGCAGATGCTACAAATATTTTATGACAGTCAAGACTTCTATCTGCTTAAAGAGCTTGAGAAGTTGGGTCCCAAAAAGGGTGTCATCAGTCAGTCAGTGAAGGATGTTGTGCAAAGCCTGGTGGATGATGATCTTGTCTTGAAAGATAAAATAGGGACTTCAGTGTACTTTTGGAGTCTTCCCAGCTGTGCTGGAAATCAGCTGAGGACTACTTACAGCAAACTGGAATCTGATCTTTCAAGCTCTAAAAAGCGCTTCATAGAGCTTGTTGAGCAGAGAGAGAATTTGAAAAGAGGCAGAGAGGACTCTGATGAGAGAGAAGCTGCTTTGGAGGAGCTGAAGGCTGTAGAGCAACACCACAAGAAGTTAAAGGTATCTACAACTCAATTGTTTCGCATTGTTTTTCAGGAGGAACTGGCTGCTTATGCTGATAGTGATCCAGCAGCACTAGAGGCAATGAATGATGCTATTGAGGTTGCTCATGCGGCAGCTAACAGATGGACAGCTTGTTTATCATCAGAACAGGCTAACGGAATTCTGAATGTAACTACAGACAATATCTTCACTCTGCAACAGTGGTGTTCGACCACTTTCCCACAAGCAAAAGAACAACTCGAACACATGTACAGGGAGGTGGGGATAACTGAAGATTTCGAATATCTGCAGTAA
CDS Sequence
  • >LOC_Os09g10850.1
    ATGTCGAAGAAGAGGGGTCTTTCTTTGGAGGAGAAGAGGGAGCAGATGCTACAAATATTTTATGACAGTCAAGACTTCTATCTGCTTAAAGAGCTTGAGAAGTTGGGTCCCAAAAAGGGTGTCATCAGTCAGTCAGTGAAGGATGTTGTGCAAAGCCTGGTGGATGATGATCTTGTCTTGAAAGATAAAATAGGGACTTCAGTGTACTTTTGGAGTCTTCCCAGCTGTGCTGGAAATCAGCTGAGGACTACTTACAGCAAACTGGAATCTGATCTTTCAAGCTCTAAAAAGCGCTTCATAGAGCTTGTTGAGCAGAGAGAGAATTTGAAAAGAGGCAGAGAGGACTCTGATGAGAGAGAAGCTGCTTTGGAGGAGCTGAAGGCTGTAGAGCAACACCACAAGAAGTTAAAGGTATCTACAACTCAATTGTTTCGCATTGTTTTTCAGGAGGAACTGGCTGCTTATGCTGATAGTGATCCAGCAGCACTAGAGGCAATGAATGATGCTATTGAGGTTGCTCATGCGGCAGCTAACAGATGGACAGCTTGTTTATCATCAGAACAGGCTAACGGAATTCTGAATGTAACTACAGACAATATCTTCACTCTGCAACAGTGGTGTTCGACCACTTTCCCACAAGCAAAAGAACAACTCGAACACATGTACAGGGAGGTGGGGATAACTGAAGATTTCGAATATCTGCAGTAA
Protein Sequence
  • >LOC_Os09g10850.1
    MSKKRGLSLEEKREQMLQIFYDSQDFYLLKELEKLGPKKGVISQSVKDVVQSLVDDDLVLKDKIGTSVYFWSLPSCAGNQLRTTYSKLESDLSSSKKRFIELVEQRENLKRGREDSDEREAALEELKAVEQHHKKLKVSTTQLFRIVFQEELAAYADSDPAALEAMNDAIEVAHAAANRWTACLSSEQANGILNVTTDNIFTLQQWCSTTFPQAKEQLEHMYREVGITEDFEYLQ*