Gene Details:
- MSU gene ID: LOC_Os09g10850
- RAPdb gene ID: Os09g0280600
- Gene Symbol: OsMFS1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Taken together, our results indicated that OsMFS1 is indispensable to the normal development of anther and embryo sacs in rice.
- Here, we characterized a completely sterile OsMFS1 (male and female sterility 1) mutant which has its pollen and embryo sacs both aborted at the reproductive stage due to severe chromosome defection.
- Map-based cloning revealed that the OsMFS1 encodes a meiotic coiled-coil protein, and it is responsible for DSB repairing that acts as an important cofactor to stimulate the single strand invasion.
- Subcellular localization analysis of OsMFS1 revealed its association with the nucleus exclusively.
- Expression pattern analyses showed the OsMFS1 was preferentially expressed in meiosis stage.
- In addition, a yeast two-hybrid (Y2H) and pull-down assay showed that OsMFS1 could physically interact with OsHOP2 protein to form a stable complex to ensure faithful homologous recombination.
Function-related keywords:
- development , pollen , anther , sterility , map-based-cloning , reproductive , nucleus , meiosis , sterile , meiotic , male-sterility , homologous-recombination
Literature:
- OsMFS1/ OsHOP2 Complex Participates in Rice Male and Female Development . DOI: 10.3389/fpls.2020.00518 ; PMID: 32499797
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os09g10850.1
ATGTCGAAGAAGAGGGGTCTTTCTTTGGAGGAGAAGAGGGAGCAGATGCTACAAATATTTTATGACAGTCAAGACTTCTATCTGCTTAAAGAGCTTGAGAAGTTGGGTCCCAAAAAGGGTGTCATCAGTCAGTCAGTGAAGGATGTTGTGCAAAGCCTGGTGGATGATGATCTTGTCTTGAAAGATAAAATAGGGACTTCAGTGTACTTTTGGAGTCTTCCCAGCTGTGCTGGAAATCAGCTGAGGACTACTTACAGCAAACTGGAATCTGATCTTTCAAGCTCTAAAAAGCGCTTCATAGAGCTTGTTGAGCAGAGAGAGAATTTGAAAAGAGGCAGAGAGGACTCTGATGAGAGAGAAGCTGCTTTGGAGGAGCTGAAGGCTGTAGAGCAACACCACAAGAAGTTAAAGGTATCTACAACTCAATTGTTTCGCATTGTTTTTCAGGAGGAACTGGCTGCTTATGCTGATAGTGATCCAGCAGCACTAGAGGCAATGAATGATGCTATTGAGGTTGCTCATGCGGCAGCTAACAGATGGACAGCTTGTTTATCATCAGAACAGGCTAACGGAATTCTGAATGTAACTACAGACAATATCTTCACTCTGCAACAGTGGTGTTCGACCACTTTCCCACAAGCAAAAGAACAACTCGAACACATGTACAGGGAGGTGGGGATAACTGAAGATTTCGAATATCTGCAGTAA
CDS Sequence
- >LOC_Os09g10850.1
ATGTCGAAGAAGAGGGGTCTTTCTTTGGAGGAGAAGAGGGAGCAGATGCTACAAATATTTTATGACAGTCAAGACTTCTATCTGCTTAAAGAGCTTGAGAAGTTGGGTCCCAAAAAGGGTGTCATCAGTCAGTCAGTGAAGGATGTTGTGCAAAGCCTGGTGGATGATGATCTTGTCTTGAAAGATAAAATAGGGACTTCAGTGTACTTTTGGAGTCTTCCCAGCTGTGCTGGAAATCAGCTGAGGACTACTTACAGCAAACTGGAATCTGATCTTTCAAGCTCTAAAAAGCGCTTCATAGAGCTTGTTGAGCAGAGAGAGAATTTGAAAAGAGGCAGAGAGGACTCTGATGAGAGAGAAGCTGCTTTGGAGGAGCTGAAGGCTGTAGAGCAACACCACAAGAAGTTAAAGGTATCTACAACTCAATTGTTTCGCATTGTTTTTCAGGAGGAACTGGCTGCTTATGCTGATAGTGATCCAGCAGCACTAGAGGCAATGAATGATGCTATTGAGGTTGCTCATGCGGCAGCTAACAGATGGACAGCTTGTTTATCATCAGAACAGGCTAACGGAATTCTGAATGTAACTACAGACAATATCTTCACTCTGCAACAGTGGTGTTCGACCACTTTCCCACAAGCAAAAGAACAACTCGAACACATGTACAGGGAGGTGGGGATAACTGAAGATTTCGAATATCTGCAGTAA
Protein Sequence
- >LOC_Os09g10850.1
MSKKRGLSLEEKREQMLQIFYDSQDFYLLKELEKLGPKKGVISQSVKDVVQSLVDDDLVLKDKIGTSVYFWSLPSCAGNQLRTTYSKLESDLSSSKKRFIELVEQRENLKRGREDSDEREAALEELKAVEQHHKKLKVSTTQLFRIVFQEELAAYADSDPAALEAMNDAIEVAHAAANRWTACLSSEQANGILNVTTDNIFTLQQWCSTTFPQAKEQLEHMYREVGITEDFEYLQ*