Gene Details:

• MSU gene ID: LOC_Os09g02650
• RAPdb gene ID: Os09g0114500
• Gene Symbol: BC12 GDD1 YD1 LGW
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Therefore, BC12/GDD1, a kinesin-like protein with transcription regulation activity, mediates cell elongation by regulating the GA biosynthesis pathway in rice.
• Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation.
• Mitotic microtubule array comparison, flow cytometric analysis and expression assays of cyclin-dependent kinase (CDK) complexes in root-tip cells showed that cell-cycle progression is affected in BC12 mutants.
• GDD1 was cloned by a map-based approach, was expressed constitutively, and was found to encode the kinesin-like protein BRITTLE CULM12 (BC12).
• BC12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition.
• Here, we report the functional characterization of Brittle Culm12 (BC12), a gene encoding a kinesin-4 protein.
• BC12 is expressed mainly in tissues undergoing cell division and secondary wall thickening.
• Therefore, BC12 functions as a dual-targeting kinesin protein and is implicated in cell-cycle progression, cellulose microfibril deposition and wall composition in the monocot plant rice.
• In this study we reveal that the novel BC12328Leu allele of BC12 modulated plant leaf color in yd1 plants, which has not been previously reported in studies of BC12/GDD1/MTD1/SRG1.
• We conclude that LGW regulates grain development by directly binding to the GW7 promoter and activating its expression.
• Overexpression of LGW increased the grain length, indicating that LGW is a positive regulator for regulating grain length.
• Elevating the GW7 expression levels in LGW plants rescued the small grain size phenotype.
• Our findings revealed that LGW plays an important role in regulating grain size, and manipulation of this gene provides a new strategy for regulating grain weight in rice.
• Protein sequence alignment analysis revealed that the mutation site was located at the nuclear localization signal (NLS) of LGW/BC12, resulting in the lgw protein not being located in the nucleus.

Function-related keywords:

• ga ,  root ,  culm ,  dwarf ,  cell-elongation ,  cell-division ,  cellulose ,  leaf ,  leaf-color ,  development ,  grain ,  grain-length ,  grain-size ,  nucleus ,  grain-weight

Literature:

• Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice .  DOI:  10.1111/j.1365-313X.2010.04238.x ;  PMID:  20444225
• Mutation of rice BC12/GDD1, which encodes a kinesin-like protein that binds to a GA biosynthesis gene promoter, leads to dwarfism with impaired cell elongation .  DOI:  10.1105/tpc.110.081901 ;  PMID:  21325138
• Characterization of a novel allele of bc12/gdd1 indicates a differential leaf color function for BC12/GDD1 in Indica and Japonica backgrounds .  DOI:  10.1016/j.plantsci.2020.110585 ;  PMID:  32771145
• Low grain weight, a new allele of BRITTLE CULM12, affects grain size through regulating GW7 expression in rice .  DOI:  10.3389/fpls.2022.997624 ;  PMID:  36176686

Related News:

Gene Resources:

• NCBI ID: AK100974
• UniProt accessions:

Sequences: