Information report for OsPIMT1
Gene Details
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Functional Descriptions
- The germination percentage of transgenic seeds overexpressing OsPIMT1 increased 9-15 % compared to the WT seeds after 21-day of artificial aging, whereas seeds from the OsPIMT1 RNAi lines overaccumulated isoAsp in embryos and experienced rapid loss of seed germinability.
- Confocal visualization of the transient expression of OsPIMTs showed that OsPIMT2 was localized in the chloroplast and nucleus, whereas OsPIMT1 was predominately found in the cytosol.
Functional Keywords
Literature and News
- Protein repair L-isoaspartyl methyltransferase 1 (PIMT1) in rice improves seed longevity by preserving embryo vigor and viability . DOI: 10.1007/s11103-015-0383-1 ; PMID: 26438231
- Rice PROTEIN l-ISOASPARTYL METHYLTRANSFERASE isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity . DOI: 10.1111/nph.13923 ; PMID: 26987457
Gene Resources
- UniProt: Q6ZJ17
- EMBL: AK059917, AK100496, AP014964
- AlphaFoldDB: Q6ZJ17
- EnsemblPlants: Os08t0557000-01
- Gramene: Os08t0557000-01
- Orthologous matrix: HMHASAC
- InterPro: IPR000682, IPR029063
- PANTHER: PTHR11579, PTHR11579:SF28
- SUPFAM: SSF53335
- Gene3D: 3.40.50.150
- OrthoDB: Q6ZJ17
- SWISS-MODEL: Q6ZJ17
- Conserved Domain Database: cd02440
- eggNOG: KOG1661
Homologs
- Arabidopsis thaliana: AT3G48330
- Panicum virgatum: Pavir.6NG368400
- Setaria viridis: SEVIR_6G251200v2
Sequences
cDNA Sequence
- >LOC_Os08g44280.2
GAGAAGAATCCATCTCTCTCTCTCTCTCCCAATCCCCCACCACCACCGCCAGCCACCAGCCACTGCCGCGGCGGGAATCCGGTTCCCGCGGCGACATCCCCCACGTCTCCGCCGCTGCACCGGCAAAGGCAGCTGCACCGCGACCCACCCGCGCGCTCCCAATCGGCCCCAAGATTAAGATTAAACCAGGAGAGACCACCCGATCGAGTGTTCTCTCACTTTCGCCATGGAGCACCGACTGTGGTCAAGCGGGGCTAGCGACAAGAACAAGGCAATGGTTGAGCAGCTCCAGAGGTACGGGATTATCAAGTCGAGCAAGGTCGCGCAGGTGATGGAGACCATCGACAGGGGGCTGTTTGTGCCTCCTGGTGCTTCTCCCTATTTCGACAGCCCGATGCCCATCGGTTACAACGCCACCATTTCTGCGCCTCACATGCACGCCTCTTGCTTGGAGCTCCTGGAGAAGCACCTTCAGCCTGGCATGCGTGCTCTGGATGTTGGATCAGGCACAGGGTACCTAACAGCTTGCTTCGCGATTATGGTTGGACCAGAAGGGCGAGCAGTTGGTGTTGAACATATCCCAGAGTTGGTTACTTCGTCTATTGAAAACATCAAGAAAAGTGCAGCGGCTCCACAGTTAACTGATGGATCCCTCAGTATACATATTACCGATGGCAGAGAAGGTTGGCCAGAGCTCGCACCCTATGATGCCATCCATGTCGGAGCTGCTGCACCGCAGATTCCACAGGCGTTGATTGAGCAGCTAAAGCCCGGTGGCAGAATGGTGATCCCAGTTGGGACTATGTTCCAGGAGCTGAAAGTGGTGGACAAGAACCAGGATGGCAAGGTCAGCATAAGAGATGAGACGGCTGTGCGCTATGTGCCCCTCACAAGCAAGGATGCGCAGCTGCAGGCTAACTGAATGCACTACCATAATATTCTGCTGAATGTAAAACCTTCAATGCGTGATCCCAGTTACATCAAGATGTTATTATTGTACATAAATTGGTCCTACCGTATGTGTGAATCGCTTGGTGCTTGTCAACAATGTTGGATGTTGGTTCGTCATGAAGCATGGAAGTATGTGCTTGTGTTGATCTCTTTTGCCTCTAATGGTGTTTCCACTACAATGTGCATGACATATTTGTTATCTTCACAACACGGTTGGCTTAGATGTAACGTATCCTGCAGACTTGATCAGCTTTGTGACATTAAAGGGATTGGAGTGTCCATGACTATTGTGGTTTCCTGTGCATTATATGAACAATAGTTTATCAAAGTATTTGGTTCTTGCAAATCCAGAGAACAACATTTCTGAAAGAGAAAAAATAAGCATAATGCATAGGGGTAAGCACTGGCTGCTGCCATGCATTTGCTCCTTTTCTCAGATATATGGTCGACAAGTGAACTTTTGGATTATCACGGAAAAAAAATACTAAGGACATCATCATTATAAATATCCTACTTGGAAAGTTGAACTCATATTTTTGAGAATAATTTAACATAACATCAGCTTGGAAGACACATAAAAAGGACGGGAGGACTCAAAATCAGGAAGCTAACGCGCGCTCTGTAAATGACAACCATCAGCTTCCGCCTTAACGCATAACACTTTAGCATTCTTGTTCAAACGTGCAGACATGCTCCCCAACGCCATATGGAATCAAAAGCCTCGCTGTGGTCGCTCAGCTGCCACATTCACTCGAAGTGGGCGGCCATCCAATTCCTGTAATCCATCATGAACAATTGAGCATAATAA - >LOC_Os08g44280.1
GAGAAGAATCCATCTCTCTCTCTCTCTCCCAATCCCCCACCACCACCGCCAGCCACCAGCCACTGCCGCGGCGGGAATCCGGTTCCCGCGGCGACATCCCCCACGTCTCCGCCGCTGCACCGGCAAAGGCAGCTGCACCGCGACCCACCCGCGCGCTCCCAATCGGCCCCAAGATTAAGATTAAACCAGGAGAGACCACCCGATCGAGTGTTCTCTCACTTTCGCCATGGAGCACCGACTGTGGTCAAGCGGGGCTAGCGACAAGAACAAGGCAATGGTTGAGCAGCTCCAGAGGTACGGGATTATCAAGTCGAGCAAGGTCGCGCAGGTGATGGAGACCATCGACAGGGGGCTGTTTGTGCCTCCTGGTGCTTCTCCCTATTTCGACAGCCCGATGCCCATCGGTTACAACGCCACCATTTCTGCGCCTCACATGCACGCCTCTTGCTTGGAGCTCCTGGAGAAGCACCTTCAGCCTGGCATGCGTGCTCTGGATGTTGGATCAGGCACAGGGTACCTAACAGCTTGCTTCGCGATTATGGTTGGACCAGAAGGGCGAGCAGTTGGTGTTGAACATATCCCAGAGTTGGTTACTTCGTCTATTGAAAACATCAAGAAAAGTGCAGCGGCTCCACAGTTAACTGATGGATCCCTCAGTATACATATTACCGATGGCAGAGAAGGTTGGCCAGAGCTCGCACCCTATGATGCCATCCATGTCGGAGCTGCTGCACCGCAGATTCCACAGGCGTTGATTGAGCAGCTAAAGCCCGGTGGCAGAATGGTGATCCCAGTTGGGACTATGTTCCAGGAGCTGAAAGTGGTGGACAAGAACCAGGATGGCAAGGTCAGCATAAGAGATGAGACGGCTGTGCGCTATGTGCCCCTCACAAGCAAGGATGCGCAGCTGCAGGCTAACTGAATGCACTACCATAATATTCTGCTGAATGTAAAACCTTCAATGCGTGATCCCAGTTACATCAAGATGTTATTATTGTACATAAATTGGTCCTACCGTATGTGTGAATCGCTTGGTGCTTGTCAACAATGTTGGATGTTGGTTCGTCATGAAGCATGGAAGTATGTGCTTGTGTTGATCTCTTTTGCCTCTAATGGTGTTTCCACTACAATGTGCATGACATATTTGTTATCTTCACAACACGGTTGGCTTAGATGTAACGTATCCTGCAGACTTGATCAGCTTTGTGACATTAAAGGGATTGGAGTGTCCATGACTATTGTGGTTTCCTGTGCATTATATGAACAATAGTTTATCAAAGTATTTGGTTCTTGCAAATCCAGAGAACAACATTTCTGAAAGAGAAAAAATAAGCATAATGCATAGGGGTAAGCACTGGCTGCTGCCATGCATTTGCTCCTTTTCTCAGATATATGGTCGACAAGTGAACTTTTGGATTATCACGGAAAAAAAATACTAAGGACATCATCATTATAAATATCCTACTTGGAAAGTTGAACTCATATTTTTGAGAATAATTTAACATAACATCAGCTTGGAAGACACATAAAAAGGACGGGAGGACTCAAAATCAGGAAGCTAACGCGCGCTCTGTAAATGACAACCATCAGCTTCCGCCTTAACGCATAACACTTTAGCATTCTTGTTCAAACGTGCAGACATGCTCCCCAACGCCATATGGAATCAAAAGCCTCGCTGTGGTCGCTCAGCTGCCACATTCACTCGAAGTGGGCGGCCATCCAATTCCTGTAATCCATCATGAACAATTGAGCATAATAA
CDS Sequence
- >LOC_Os08g44280.2
ATGGAGCACCGACTGTGGTCAAGCGGGGCTAGCGACAAGAACAAGGCAATGGTTGAGCAGCTCCAGAGGTACGGGATTATCAAGTCGAGCAAGGTCGCGCAGGTGATGGAGACCATCGACAGGGGGCTGTTTGTGCCTCCTGGTGCTTCTCCCTATTTCGACAGCCCGATGCCCATCGGTTACAACGCCACCATTTCTGCGCCTCACATGCACGCCTCTTGCTTGGAGCTCCTGGAGAAGCACCTTCAGCCTGGCATGCGTGCTCTGGATGTTGGATCAGGCACAGGGTACCTAACAGCTTGCTTCGCGATTATGGTTGGACCAGAAGGGCGAGCAGTTGGTGTTGAACATATCCCAGAGTTGGTTACTTCGTCTATTGAAAACATCAAGAAAAGTGCAGCGGCTCCACAGTTAACTGATGGATCCCTCAGTATACATATTACCGATGGCAGAGAAGGTTGGCCAGAGCTCGCACCCTATGATGCCATCCATGTCGGAGCTGCTGCACCGCAGATTCCACAGGCGTTGATTGAGCAGCTAAAGCCCGGTGGCAGAATGGTGATCCCAGTTGGGACTATGTTCCAGGAGCTGAAAGTGGTGGACAAGAACCAGGATGGCAAGGTCAGCATAAGAGATGAGACGGCTGTGCGCTATGTGCCCCTCACAAGCAAGGATGCGCAGCTGCAGGCTAACTGA - >LOC_Os08g44280.1
ATGGAGCACCGACTGTGGTCAAGCGGGGCTAGCGACAAGAACAAGGCAATGGTTGAGCAGCTCCAGAGGTACGGGATTATCAAGTCGAGCAAGGTCGCGCAGGTGATGGAGACCATCGACAGGGGGCTGTTTGTGCCTCCTGGTGCTTCTCCCTATTTCGACAGCCCGATGCCCATCGGTTACAACGCCACCATTTCTGCGCCTCACATGCACGCCTCTTGCTTGGAGCTCCTGGAGAAGCACCTTCAGCCTGGCATGCGTGCTCTGGATGTTGGATCAGGCACAGGGTACCTAACAGCTTGCTTCGCGATTATGGTTGGACCAGAAGGGCGAGCAGTTGGTGTTGAACATATCCCAGAGTTGGTTACTTCGTCTATTGAAAACATCAAGAAAAGTGCAGCGGCTCCACAGTTAACTGATGGATCCCTCAGTATACATATTACCGATGGCAGAGAAGGTTGGCCAGAGCTCGCACCCTATGATGCCATCCATGTCGGAGCTGCTGCACCGCAGATTCCACAGGCGTTGATTGAGCAGCTAAAGCCCGGTGGCAGAATGGTGATCCCAGTTGGGACTATGTTCCAGGAGCTGAAAGTGGTGGACAAGAACCAGGATGGCAAGGTCAGCATAAGAGATGAGACGGCTGTGCGCTATGTGCCCCTCACAAGCAAGGATGCGCAGCTGCAGGCTAACTGA
Protein Sequence
- >LOC_Os08g44280.2
MEHRLWSSGASDKNKAMVEQLQRYGIIKSSKVAQVMETIDRGLFVPPGASPYFDSPMPIGYNATISAPHMHASCLELLEKHLQPGMRALDVGSGTGYLTACFAIMVGPEGRAVGVEHIPELVTSSIENIKKSAAAPQLTDGSLSIHITDGREGWPELAPYDAIHVGAAAPQIPQALIEQLKPGGRMVIPVGTMFQELKVVDKNQDGKVSIRDETAVRYVPLTSKDAQLQAN* - >LOC_Os08g44280.1
MEHRLWSSGASDKNKAMVEQLQRYGIIKSSKVAQVMETIDRGLFVPPGASPYFDSPMPIGYNATISAPHMHASCLELLEKHLQPGMRALDVGSGTGYLTACFAIMVGPEGRAVGVEHIPELVTSSIENIKKSAAAPQLTDGSLSIHITDGREGWPELAPYDAIHVGAAAPQIPQALIEQLKPGGRMVIPVGTMFQELKVVDKNQDGKVSIRDETAVRYVPLTSKDAQLQAN*