Gene Details:
- MSU gene ID: LOC_Os08g42750
- RAPdb gene ID: Os08g0540400
- Gene Symbol: OsCPK21
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- These results suggest that OsCPK21 is involved in the positive regulation of the signaling pathways that are involved in the response to ABA and salt stress.
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice.
- The survival rate of the OsCPK21-FOX plants was higher than that of wild-type (WT) plants grown under high salinity conditions.
- Several ABA- and high salinity-inducible genes were more highly expressed in the OsCPK21-FOX plants than in WT plants.
- The inhibition of seedling growth by abscisic acid (ABA) treatment was greater in the OsCPK21-FOX plants than in WT plants.
- These results suggest that OsCPK21 phosphorylates OsGF14e to facilitate the response to ABA and salt stress.
- We used an in<U+00A0>vitro kinase assay and site-directed mutagenesis to verify that OsCPK21 phosphorylates OsGF14e at Tyr-138.
- Microarray analysis and qRT-PCR revealed that the transcription of OsCPK21 is coordinated with that of MIKC*-type MADS box transcription factors OsMADS62, OsMADS63, and OsMADS68 during rice anther development.
- OsCPK21 is required for pollen late-stage development in rice.
- Here, we found that the knockdown of spikelet-specific OsCPK21 causes pollen abortion in OsCPK21-RNAi transgenic plants.
Function-related keywords:
- salt , salinity , salt-tolerance , seedling , ABA , growth , salt-stress , stress , Kinase , transcription-factor , pollen , anther , development , anther-development
Literature:
- Functional characterisation of OsCPK21, a calcium-dependent protein kinase that confers salt tolerance in rice . DOI: 10.1007/s11103-010-9717-1 ; PMID: 21136139
- Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice . DOI: 10.1016/j.bbrc.2017.09.166 ; PMID: 28988107
- OsCPK21 is required for pollen late-stage development in rice . DOI: 10.1016/j.jplph.2019.153000 ; PMID: 31220626
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os08g42750.1
ATGCACTAATTAAAAAGCCCACACGCGACGCGACACGCCCGGCGAGCCGCGCGAGCGCGACACACACAGAGGAGGAGGCCGCGCGCGCGACGCGAGCTCGCCCTCTGCGTGCGCCGCCGCGAGGTCGCCGCCGCCGCCGCCGCCGTCGATCGGCGCCCGATCGATGGGGGGCTGCTACTCCGCCTACGCCTCCTCGCGCAAGCTGCGCGGCCGCATCAGCAAGATCTCCCTCGTCATCCCCGACCCGGTCCCCGACGCCGAGGCCGCCTCGCCGCGCAAGGACGGCGTCGATGGCGACGGCGACGACGTGAGGGGTGGTGGTGGTGGCTGTGACGATGGCGGTGACGTCGTCGCCATCGCGACGACGACGGCGGACGAGTTCGCGCGGCGGTACGTGCTGGGGAAGGAGCTGGGGCGCGGGGAGTTCGGGGTGACGCGGCGGTGCAGCGACGCGGCGACCGGGGAGGCGCTGGCGTGCAAGACGATCCGGAAGCACAGGCGCCTGGCGCCGCCGCGGGTGACCGCGGCGAAGGCGGCGGCCGCGCACGGGGAGGACGTGAAGAGGGAGGTGGCCATCATGCGGCGCATGTCGTCGGCGTCGTCGTCGCGCGGGGGCGGCGCCGCGTCGTCCGCCGCCGTGGTGCGGCTCCGCGAGGCATGCGAGGACGCCGCCGACGGCTCCGTCCACCTCGTCATGGAGCTCTGCGAGGGCGGCGAGCTGTTCGACCGCATCGTGGCGCGCGGACACTACTCCGAGCGCGCCGCCGCCAACATCTTCCGCACCATCGTCGACGTCGTCCAGCTGTGCCACTCGAACGGGGTGATACACCGCGATCTGAAGCCGGAGAACTTCCTGTTCGCGAACAAATCGGAGGACTCGCCGCTCAAGGTCATCGACTTCGGCCTCTCGGTGTTCTTCAAGCCAGGCGACCGGTTCACGGAGGTGGTGGGGAGCGCGTACTACATGGCGCCGGAGGTATTGCGGCGGAGCTACGGGCCGGAGGTGGACGTGTGGAGCGCCGGCGTCATCCTCTACATCCTCCTCTGCGGCGTCCCTCCATTCTGGGGAGACAACGACGAGAAGATCGCGCAGGCGATCCTCCGCGGCGCCATCGACTTCAACAGGGAGCCATTGCCGAGGGTCTCCGCCAACGCCAAGGACCTCGTCAGGAGGATGCTTGATCCTAACCCATCCACCCGCCTGACGGCCAAACAAGTTCTTGAGCATCCATGGCTGAAGAACGCGGACACGGCGCCGAACGTGTCGCTGGGCGACGCCGTGCGGGCGAGGCTGCAGCAGTTCTCCGCCATGAACAAGTTCAAGAAGAAGGCTCTCGGAGTGGTGGCGCGGAACCTGCCGGGGGAGGAGGTGGACAAGTACGTGCAGATGTTCCACCACATGGACAAGGACAAGAACGGGCACCTGTCGCTCGACGAACTCTTGGAAGGCCTCCACATCAACGGCCAGCCCGTCCCCGAGCCCGAGATCAGGATGCTACTCGAAGCCGCGGACACGGACGGGAACGGGACGCTGGACTGCGACGAGTTCGTGACGGTGTCGGTGCACCTGAAGAAGATGAGCAACGACGAGTACCTGGCGGCGGCGTTCAACTACTTCGACAAGGACGGCAGCGGGTTCATCGAGCTGGACGAGCTGCGGGAGGAGGTGGGCCCAAACGAGCAGGCCATCCTGGAGATCCTCCGCGACGTCGACACCGACAAGGACGGCCGCATCAGCTACCAGGAGTTCGAGCTCATGATGAAGTCCGGCGCCGACTGGAGGAACGCCTCCAGGCACTTCTCCAGGGCCAACTTCAGCACCCTCAGCAGGAGGCTCTGCAAGGATACTCTTACTCCCTGATGATCGATGATGCCCAACAATCTCATCAACTTTGTTTCATCAAGGCTTATAAACTGCACATCTTTCACCTTCACCAGACAGGGATAACTAGAGAGAGATACAAGACCAACTTAGAGAAATGTATTGGAGTGGCAACACGATGCGATGCTGCGCTTGGATTGTGAAATGAGAAATGGAACTGAAATGGGGATCGTACCGGTTTTGCTACTCTTTCGGTAAATGGGTATTTTTGTTTGCTATAATATGCACACAACATCAAAAATTCCATCCATCAAAATATGCAGCTCGAACGACGAATCTATAAGAAATCAGGTACTGCTGAGTAAACACCAGTTTCCCACATGATTTATCAACCATAGATTATGACGAGCTTTGGTAATTTACATGACCGCTCAATAGATAAGAGCTACATCTACCATTCTCCAGCTTCACAGGTTGGTCAGTACAGGGGGTGGAACTTTACCCACCACATCATCTCATGTCATTTAAACAAAAACAGAACATTGCCCACCACATTAACACAACTACAGACCATAACTAATATGTACATAATATAGGCTAGAAAGTAAAAAAAAAAAAGACTACCACATGTTTGTACCCCATCCACCTTCGCTCCATAGTCGGTCCTGCATTGTTGCTGCAATCTAGATACAACCAGGGACCTTCACTCACACATGTACGCCATTGCTGCCATTGGAAGCTCTTCTTCAACGCCACATAATTTAGGAACAGAACTACAATCAACAATCTTCTCATGAGCTTTGAGTAAGCACCCACCTTCTCAACAGCTTCGAGATTTATGGGGGGCACTGGCTTTCAGCCAGTTTTTCATCTAGTATCTATGCTTATCGCAGACGCTGCATGCATCCACCGATGAAGGGTTAATAAACGTGCAGCGCTCACAAGGCCAGCCTTCATCTGAGTTGTCCGAAGATGTTCTAGGAAGCTGCTTCTTTGTTTTGCCTTTAGTGACTGCTTTCTTGGAGGGGATGTTGGTGGCATTGCTTGATGTGGTACCCTGGGCAGTAGAGTGTACATCCTCCAAGCCAGCCTCCAGTGCTTGAACAAGGTTCTCAAAATAATTCCGAGTCACACT
CDS Sequence
- >LOC_Os08g42750.1
ATGGGGGGCTGCTACTCCGCCTACGCCTCCTCGCGCAAGCTGCGCGGCCGCATCAGCAAGATCTCCCTCGTCATCCCCGACCCGGTCCCCGACGCCGAGGCCGCCTCGCCGCGCAAGGACGGCGTCGATGGCGACGGCGACGACGTGAGGGGTGGTGGTGGTGGCTGTGACGATGGCGGTGACGTCGTCGCCATCGCGACGACGACGGCGGACGAGTTCGCGCGGCGGTACGTGCTGGGGAAGGAGCTGGGGCGCGGGGAGTTCGGGGTGACGCGGCGGTGCAGCGACGCGGCGACCGGGGAGGCGCTGGCGTGCAAGACGATCCGGAAGCACAGGCGCCTGGCGCCGCCGCGGGTGACCGCGGCGAAGGCGGCGGCCGCGCACGGGGAGGACGTGAAGAGGGAGGTGGCCATCATGCGGCGCATGTCGTCGGCGTCGTCGTCGCGCGGGGGCGGCGCCGCGTCGTCCGCCGCCGTGGTGCGGCTCCGCGAGGCATGCGAGGACGCCGCCGACGGCTCCGTCCACCTCGTCATGGAGCTCTGCGAGGGCGGCGAGCTGTTCGACCGCATCGTGGCGCGCGGACACTACTCCGAGCGCGCCGCCGCCAACATCTTCCGCACCATCGTCGACGTCGTCCAGCTGTGCCACTCGAACGGGGTGATACACCGCGATCTGAAGCCGGAGAACTTCCTGTTCGCGAACAAATCGGAGGACTCGCCGCTCAAGGTCATCGACTTCGGCCTCTCGGTGTTCTTCAAGCCAGGCGACCGGTTCACGGAGGTGGTGGGGAGCGCGTACTACATGGCGCCGGAGGTATTGCGGCGGAGCTACGGGCCGGAGGTGGACGTGTGGAGCGCCGGCGTCATCCTCTACATCCTCCTCTGCGGCGTCCCTCCATTCTGGGGAGACAACGACGAGAAGATCGCGCAGGCGATCCTCCGCGGCGCCATCGACTTCAACAGGGAGCCATTGCCGAGGGTCTCCGCCAACGCCAAGGACCTCGTCAGGAGGATGCTTGATCCTAACCCATCCACCCGCCTGACGGCCAAACAAGTTCTTGAGCATCCATGGCTGAAGAACGCGGACACGGCGCCGAACGTGTCGCTGGGCGACGCCGTGCGGGCGAGGCTGCAGCAGTTCTCCGCCATGAACAAGTTCAAGAAGAAGGCTCTCGGAGTGGTGGCGCGGAACCTGCCGGGGGAGGAGGTGGACAAGTACGTGCAGATGTTCCACCACATGGACAAGGACAAGAACGGGCACCTGTCGCTCGACGAACTCTTGGAAGGCCTCCACATCAACGGCCAGCCCGTCCCCGAGCCCGAGATCAGGATGCTACTCGAAGCCGCGGACACGGACGGGAACGGGACGCTGGACTGCGACGAGTTCGTGACGGTGTCGGTGCACCTGAAGAAGATGAGCAACGACGAGTACCTGGCGGCGGCGTTCAACTACTTCGACAAGGACGGCAGCGGGTTCATCGAGCTGGACGAGCTGCGGGAGGAGGTGGGCCCAAACGAGCAGGCCATCCTGGAGATCCTCCGCGACGTCGACACCGACAAGGACGGCCGCATCAGCTACCAGGAGTTCGAGCTCATGATGAAGTCCGGCGCCGACTGGAGGAACGCCTCCAGGCACTTCTCCAGGGCCAACTTCAGCACCCTCAGCAGGAGGCTCTGCAAGGATACTCTTACTCCCTGA
Protein Sequence
- >LOC_Os08g42750.1
MGGCYSAYASSRKLRGRISKISLVIPDPVPDAEAASPRKDGVDGDGDDVRGGGGGCDDGGDVVAIATTTADEFARRYVLGKELGRGEFGVTRRCSDAATGEALACKTIRKHRRLAPPRVTAAKAAAAHGEDVKREVAIMRRMSSASSSRGGGAASSAAVVRLREACEDAADGSVHLVMELCEGGELFDRIVARGHYSERAAANIFRTIVDVVQLCHSNGVIHRDLKPENFLFANKSEDSPLKVIDFGLSVFFKPGDRFTEVVGSAYYMAPEVLRRSYGPEVDVWSAGVILYILLCGVPPFWGDNDEKIAQAILRGAIDFNREPLPRVSANAKDLVRRMLDPNPSTRLTAKQVLEHPWLKNADTAPNVSLGDAVRARLQQFSAMNKFKKKALGVVARNLPGEEVDKYVQMFHHMDKDKNGHLSLDELLEGLHINGQPVPEPEIRMLLEAADTDGNGTLDCDEFVTVSVHLKKMSNDEYLAAAFNYFDKDGSGFIELDELREEVGPNEQAILEILRDVDTDKDGRISYQEFELMMKSGADWRNASRHFSRANFSTLSRRLCKDTLTP*