Information report for OsHI-LOX;OsLOX9
Gene Details
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Functional Descriptions
- We cloned a chloroplast-localized type 2 13-lipoxygenase gene of rice, OsHI-LOX, whose transcripts were up-regulated in response to feeding by the rice striped stem borer (SSB) Chilo suppressalis and the rice brown planthopper (BPH) Niaparvata lugens, as well as by mechanical wounding and treatment with JA.
- The antisense expression of OsNPR1 (as-npr1), which reduced the expression of the gene by 50%, increased elicited levels of JA and ethylene (ET) as well as of expression of a lipoxygenase gene OsHI-LOX and an ACC synthase gene OsACS2.
- Antisense expression of OsHI-LOX (as-lox) reduced SSB- or BPH-induced JA and trypsin protease inhibitor (TrypPI) levels, improved the larval performance of SBB as well as that of the rice leaf folder (LF) Cnaphalocrocis medinalis, and increased the damage caused by SSB and LF larvae.
- These results imply that OsHI-LOX is involved in herbivore-induced JA biosynthesis, and plays contrasting roles in controlling rice resistance to chewing and phloem-feeding herbivores.
- Silencing OsMPK3 (ir-mpk3) reduced the expression of the gene by 50-70 %, decreased elicited levels of JA and diminished the expression of a lipoxygenase gene OsHI-LOX and an allene oxide synthase gene OsAOS1.
- Antisense expression of OsPLDalpha4 and -alpha5 (as-pld), which resulted in a 50% reduction of the expression of the two genes, reduced elicited levels of linolenic acid, JA, green leaf volatiles, and ethylene and attenuated the SSB-induced expression of a mitogen-activated protein kinase (OsMPK3), a lipoxygenase (OsHI-LOX), a hydroperoxide lyase (OsHPL3), as well as a 1-aminocyclopropane-1-carboxylic acid synthase (OsACS2).
Functional Keywords
- brown-planthopper , ethylene , leaf , ja , chloroplast , stem
Literature and News
- Silencing OsHI-LOX makes rice more susceptible to chewing herbivores, but enhances resistance to a phloem feeder . DOI: 10.1111/j.1365-313X.2009.03988.x ; PMID: 19656341
- The chloroplast-localized phospholipases D α4 and α5 regulate herbivore-induced direct and indirect defenses in rice . DOI: 10.1104/pp.111.183749 ; PMID: 21984727
- OsNPR1 negatively regulates herbivore-induced JA and ethylene signaling and plant resistance to a chewing herbivore in rice . DOI: 10.1111/j.1399-3054.2012.01666.x ; PMID: 22694163
- OsMPK3 positively regulates the JA signaling pathway and plant resistance to a chewing herbivore in rice . DOI: 10.1007/s00299-013-1389-2 ; PMID: 23344857
- Novel crosstalk between ethylene- and jasmonic acid-pathway responses to a piercing-sucking insect in rice . DOI: 10.1111/nph.16111 ; PMID: 31407341
Gene Resources
- UniProt: D1G597
- EMBL: FJ607153
- AlphaFoldDB: D1G597
- EnsemblPlants: Os08t0508800-01
- Gramene: Os08t0508800-01
- Orthologous matrix: ANIQSRH
- InterPro: IPR000907, IPR001024, IPR001246
- PANTHER: PTHR11771
- SUPFAM: SSF48484, SSF49723
- PROSITE: PS00081, PS00711, PS50095
- Gene3D: 1.20.245.10, 2.60.60.20, 3.10.450.60
- OrthoDB: D1G597
- SWISS-MODEL: D1G597
- Conserved Domain Database: cd01751
Sequences
cDNA Sequence
- >LOC_Os08g39840.1
TTAATTAGCTCGATCTGCAAGCAATCCAAAGCCAACCCAAGCCAAGCTCACGAGTGCAGAGCTAGACGACGTGTAGATACGTAGTCTCCTCCGATATCTCTCTCTCTCTCGATCGACGCCTCTGCATATATAATACCCATACCATGTTGCGTCCTCAGCTCAATCCATCTAGCCACACGACGACGACGAGCAGCAGCAGCAGCACGCAGCTGTTCGCATCCTCGTCGTGCATCGCTAGCCTTCGCCGGCCGTCGTCGTCGTCGTCGTCGGTGGTCGCCGCCGCACGCCGGACGCGGGGGCAAGGTAGCAGTCGGGTTGTTGTTGTGTGCGCGTCGTCGTCGGCGACGGCGAGCAGGGGAGATAGTTCTTCGGACATGGCGGCGGCGGCGGCGGTGCGGGTGAAGGCGGTGGCGACGATCAAGGTCACCGTCGGCGAGTTGATCAACAGGTCGATCGACATCAGGGATCTCATCGGCAGGTCGCTCTCCCTCGAGCTCGTCAGCTCCGAGCTTGACGCGAAGACCGGGAAGGAGAAAGCAACTGTGCGGAGCTACGCGCACAATGTGGACGACGACGATCATAGCGTCGTCACCTACGAGGCCGACTTCGACGTGCCGAGTGGATTCGGCCCCATCGGCGCCATCATCGTCACCAACGAACTCCGGCAGGAGATGTTCCTCGAGGACATCAACCTCACCGCCAGCGATGGCGCCGGCAACTCCACTGTCCTCCCCATCCGCTGCAACTCCTGGGTCCAACCCAAGTCCGTCGGCGATGAGGGCACGCCTAGCAAACGCATCTTCTTCGCCAACAAGACTTACTTGCCGGGACAGACGCCGGCGGGGCTCCGGAGCTACCGGAAGAATGACCTCCAGCAGAAGCGCGGTGACGGCACTGGCGAGAGGGAGGCCGACGACCGTGTCTACGACTACGACGTTTACAACGACCTCGGTAACCCGGACAGCAACGGCGATCTCGCCCGCCCCGTCCTTGGCGGCAACAAGCAGTTCCCCTACCCTCGCCGCTGCCGCACCGGCCGCCCCCCCTCCAAAAAAGACCCTAAGTCGGAGACGAGGAAGGGCAACGTGTACGTGCCGAGGGACGAGGAGTTCTCACCGGAGAAGGAGGACTACTTCCTCCGCAAGACGGTGGGGTCGGTGCTCCAGGCCGCCGTGCCGGCGGCGCAGTCGCTGCTCCTCGACAAGCTGAAATGGAACCTTCCGTTCCCGTCCTTCTTCGTCATCGACAAGCTGTTCGAGGACGGCGTCGAGCTTCCCGGCGTCGACAAGCTCAACTTCCTCGAGAGCGTCGTGCCCCGCCTGCTCGAACACCTCCGCGACACCCCCGCCGAGAAGATCCTCCGCTTCGAAACTCCGGCCAACATCCAAAAGGACAAGTTCGCATGGCTCAGAGACGAGGAGTTCGCGAGGGAAACGCTCGCTGGCATCAACCCGTACGCCATCGAGCTCGTCAGGGAATTTCCGCTGAAGAGCAAGCTCGACCCGGCGGTGTACGGTCCGGCGGAGTCGGCGATCACCGCCGATTTGCTGGAGGAGCAGATGAGGCGCGTGATGACGGTGGAGGAGGCGATCAGCCAGAAGAGGCTGTTCATGCTCGACTTCCATGACCTCTTCTTGCCGTACGTGCACAAGATCCGGTCGCTGGATCACACCACCATGTACGGCTCGCGCACCGTCTTCTTCCTCACCGACGACGGCACGCTGCAGCTGCTCGCCATCGAGCTCACCCGGCCGGCCTCGCCGTCGCAGCCGCAGTGGCGGCAGGTGTTCACGCCGTCCACGGACGCCACCATGTCGTGGCTGTGGCGGATGGCCAAGGCCCACGTCCGCGCCCACGACGCCGGCCACCACGAGCTCATCACCCACTGGCTGCGCACGCACTGCGCGGTGGAGCCATACATCATCGCGGCGAACCGGCAGCTCAGCGAGATGCACCCCATCTACCAGCTGCTGCGCCCGCACTTCCGCTACACGATGCGGATCAACGCGCGCGCCCGCTCGGCGCTGATCAGCGCCGGCGGCATCATCGAGCGATCCTTCTCGCCGCAGAAGTACTCCATGGAGCTCAGCTCCGTCGCCTACGACAAGCTCTGGCGCTTCGACACGGAGGCGCTCCCCGCCGACCTCGTCCGCCGCGGCATGGCCGAGGAGGACCCCACGGCGGAGCACGGCCTCAAGCTCGCCATCGAGGACTACCCGTTCGCCAACGACGGCCTCCTCATCTGGGACGCCATCAAGACCTGGGTCCAGGCGTACGTCGCGCGGTTCTACCCCGACGCCGACAGCGTCGCCGGCGACGAGGAGCTCCAGGCGTTCTGGACCGAGGTGCGCACCAAGGGGCACGGCGACAAGAAGGACGCCCCGTGGTGGCCGAAGTTGGACTCGCCGGAGAGCCTCGCCCACACGCTGACCACCATCGTCTGGGTGGCGGCGGCGCACCACGCCGCCGTCAACTTCGGGCAGTACGACTTCGGCGGCTACTTCCCCAACCGGCCGTCCATCGCGCGCACGGTCATGCCGGTGGAGGAGCCCGTGGACGGCGCCGCCATGGAGAGGTTCCTGGACAACCCGGACCAGGCGCTCCGCGAGTGCTTCCCGTCACAGGTGCAGGCGACGGTGGTGATGGCGGTGCTCGACGTGCTGTCCAGCCACTCCACCGACGAGGAGTACCTCGGCGGCGAGCAGACGAGGCCGTGGAACAGCGACGCGGCGGTGCAGGCGGCGTACGACGGGTTCGCAGCCCGGCTCAAGGAGATCGAGGGCGTCATCGATGGCCGGAACAAGGATAGAAAGCTCAAGAACAGGTGCGGCGCCGGCATCCTGCCGTACCAGCTGATGAAGCCCTTCTCCGACTCCGGCGTCACCGGCATGGGCATCCCCAACAGCACATCCATCTGATGAGACAAAACGCTCCAAAACTACTTGCAAATTGATCATTCATCATCTACTGTTTTAACCAATATGTCACTCCCAAATCTTTATTGATTTTTATCATGTTTGAAAAGATAATCGAGAGGTATCATACCATTTTTATAGTCTAAAATTTAGATATTATGTATCTCAAGGTATCACAATGTTTAATGTAAATAAATTTTGTTACCTCAAGATATTTTCTTAGCGACTGTAATTTTTTTCCTCTGTTGGCACCATTCTTATCTGCCTTTTAACAGAGCTAGTACTTTCTATATGAAGATCTTTTAAAAAAACAAATTAATAAATCTATTTTTAATT
CDS Sequence
- >LOC_Os08g39840.1
ATGTTGCGTCCTCAGCTCAATCCATCTAGCCACACGACGACGACGAGCAGCAGCAGCAGCACGCAGCTGTTCGCATCCTCGTCGTGCATCGCTAGCCTTCGCCGGCCGTCGTCGTCGTCGTCGTCGGTGGTCGCCGCCGCACGCCGGACGCGGGGGCAAGGTAGCAGTCGGGTTGTTGTTGTGTGCGCGTCGTCGTCGGCGACGGCGAGCAGGGGAGATAGTTCTTCGGACATGGCGGCGGCGGCGGCGGTGCGGGTGAAGGCGGTGGCGACGATCAAGGTCACCGTCGGCGAGTTGATCAACAGGTCGATCGACATCAGGGATCTCATCGGCAGGTCGCTCTCCCTCGAGCTCGTCAGCTCCGAGCTTGACGCGAAGACCGGGAAGGAGAAAGCAACTGTGCGGAGCTACGCGCACAATGTGGACGACGACGATCATAGCGTCGTCACCTACGAGGCCGACTTCGACGTGCCGAGTGGATTCGGCCCCATCGGCGCCATCATCGTCACCAACGAACTCCGGCAGGAGATGTTCCTCGAGGACATCAACCTCACCGCCAGCGATGGCGCCGGCAACTCCACTGTCCTCCCCATCCGCTGCAACTCCTGGGTCCAACCCAAGTCCGTCGGCGATGAGGGCACGCCTAGCAAACGCATCTTCTTCGCCAACAAGACTTACTTGCCGGGACAGACGCCGGCGGGGCTCCGGAGCTACCGGAAGAATGACCTCCAGCAGAAGCGCGGTGACGGCACTGGCGAGAGGGAGGCCGACGACCGTGTCTACGACTACGACGTTTACAACGACCTCGGTAACCCGGACAGCAACGGCGATCTCGCCCGCCCCGTCCTTGGCGGCAACAAGCAGTTCCCCTACCCTCGCCGCTGCCGCACCGGCCGCCCCCCCTCCAAAAAAGACCCTAAGTCGGAGACGAGGAAGGGCAACGTGTACGTGCCGAGGGACGAGGAGTTCTCACCGGAGAAGGAGGACTACTTCCTCCGCAAGACGGTGGGGTCGGTGCTCCAGGCCGCCGTGCCGGCGGCGCAGTCGCTGCTCCTCGACAAGCTGAAATGGAACCTTCCGTTCCCGTCCTTCTTCGTCATCGACAAGCTGTTCGAGGACGGCGTCGAGCTTCCCGGCGTCGACAAGCTCAACTTCCTCGAGAGCGTCGTGCCCCGCCTGCTCGAACACCTCCGCGACACCCCCGCCGAGAAGATCCTCCGCTTCGAAACTCCGGCCAACATCCAAAAGGACAAGTTCGCATGGCTCAGAGACGAGGAGTTCGCGAGGGAAACGCTCGCTGGCATCAACCCGTACGCCATCGAGCTCGTCAGGGAATTTCCGCTGAAGAGCAAGCTCGACCCGGCGGTGTACGGTCCGGCGGAGTCGGCGATCACCGCCGATTTGCTGGAGGAGCAGATGAGGCGCGTGATGACGGTGGAGGAGGCGATCAGCCAGAAGAGGCTGTTCATGCTCGACTTCCATGACCTCTTCTTGCCGTACGTGCACAAGATCCGGTCGCTGGATCACACCACCATGTACGGCTCGCGCACCGTCTTCTTCCTCACCGACGACGGCACGCTGCAGCTGCTCGCCATCGAGCTCACCCGGCCGGCCTCGCCGTCGCAGCCGCAGTGGCGGCAGGTGTTCACGCCGTCCACGGACGCCACCATGTCGTGGCTGTGGCGGATGGCCAAGGCCCACGTCCGCGCCCACGACGCCGGCCACCACGAGCTCATCACCCACTGGCTGCGCACGCACTGCGCGGTGGAGCCATACATCATCGCGGCGAACCGGCAGCTCAGCGAGATGCACCCCATCTACCAGCTGCTGCGCCCGCACTTCCGCTACACGATGCGGATCAACGCGCGCGCCCGCTCGGCGCTGATCAGCGCCGGCGGCATCATCGAGCGATCCTTCTCGCCGCAGAAGTACTCCATGGAGCTCAGCTCCGTCGCCTACGACAAGCTCTGGCGCTTCGACACGGAGGCGCTCCCCGCCGACCTCGTCCGCCGCGGCATGGCCGAGGAGGACCCCACGGCGGAGCACGGCCTCAAGCTCGCCATCGAGGACTACCCGTTCGCCAACGACGGCCTCCTCATCTGGGACGCCATCAAGACCTGGGTCCAGGCGTACGTCGCGCGGTTCTACCCCGACGCCGACAGCGTCGCCGGCGACGAGGAGCTCCAGGCGTTCTGGACCGAGGTGCGCACCAAGGGGCACGGCGACAAGAAGGACGCCCCGTGGTGGCCGAAGTTGGACTCGCCGGAGAGCCTCGCCCACACGCTGACCACCATCGTCTGGGTGGCGGCGGCGCACCACGCCGCCGTCAACTTCGGGCAGTACGACTTCGGCGGCTACTTCCCCAACCGGCCGTCCATCGCGCGCACGGTCATGCCGGTGGAGGAGCCCGTGGACGGCGCCGCCATGGAGAGGTTCCTGGACAACCCGGACCAGGCGCTCCGCGAGTGCTTCCCGTCACAGGTGCAGGCGACGGTGGTGATGGCGGTGCTCGACGTGCTGTCCAGCCACTCCACCGACGAGGAGTACCTCGGCGGCGAGCAGACGAGGCCGTGGAACAGCGACGCGGCGGTGCAGGCGGCGTACGACGGGTTCGCAGCCCGGCTCAAGGAGATCGAGGGCGTCATCGATGGCCGGAACAAGGATAGAAAGCTCAAGAACAGGTGCGGCGCCGGCATCCTGCCGTACCAGCTGATGAAGCCCTTCTCCGACTCCGGCGTCACCGGCATGGGCATCCCCAACAGCACATCCATCTGA
Protein Sequence
- >LOC_Os08g39840.1
MLRPQLNPSSHTTTTSSSSSTQLFASSSCIASLRRPSSSSSSVVAAARRTRGQGSSRVVVVCASSSATASRGDSSSDMAAAAAVRVKAVATIKVTVGELINRSIDIRDLIGRSLSLELVSSELDAKTGKEKATVRSYAHNVDDDDHSVVTYEADFDVPSGFGPIGAIIVTNELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVGDEGTPSKRIFFANKTYLPGQTPAGLRSYRKNDLQQKRGDGTGEREADDRVYDYDVYNDLGNPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETRKGNVYVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLFEDGVELPGVDKLNFLESVVPRLLEHLRDTPAEKILRFETPANIQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLDHTTMYGSRTVFFLTDDGTLQLLAIELTRPASPSQPQWRQVFTPSTDATMSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLRPHFRYTMRINARARSALISAGGIIERSFSPQKYSMELSSVAYDKLWRFDTEALPADLVRRGMAEEDPTAEHGLKLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSSHSTDEEYLGGEQTRPWNSDAAVQAAYDGFAARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDSGVTGMGIPNSTSI*