Gene Details:

• MSU gene ID: LOC_Os08g38990
• RAPdb gene ID: Os08g0499300
• Gene Symbol: OsWRKY30
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Overexpression of OsWRKY30 in rice dramatically increased drought tolerance.
• Overexpression of OsWRKY30AA, in which all SP (serine residue followed by proline residue) sites were replaced by AP (A, alanine), resulted in no improvement in drought tolerance.
• OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice.
• OsWRKY30 and OsWRKY83 responded to both SA- and JA treatments.
• Here we report involvement of a poorly characterized group C MAPK of rice namely, OsMPK7 along with its upstream MAPK kinase, OsMKK3 and downstream target, OsWRKY30 during Xanthomonas oryzae infection, a causal agent of leaf blight disease in rice.
• Furthermore, OsWRKY30 was identified as downstream target of OsMPK7 through protein-protein interaction techniques and was found to be a positive regulator of defence response against X.
• OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak.
• Genetic assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc, knocking out OsWRKY30 in the oslic mutants attenuated the resistance against bacterial pathogens.
• Collectively, these results reveal that OsMAPK6-mediated phosphorylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription, suggesting that OsMAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens.

Function-related keywords:

• drought ,  drought-tolerance ,  ja ,  leaf ,  disease ,  blight ,  defence ,  defence-response ,  blight-disease ,  resistance ,  bacterial-blight ,  R-protein ,  zinc ,  xoo ,  Xoo

Literature:

• A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response .  DOI:  10.1007/s00299-006-0138-1 ;  PMID:  16528562
• Constitutive expression of rice WRKY30 gene increases the endogenous jasmonic acid accumulation, PR gene expression and resistance to fungal pathogens in rice .  DOI:  10.1007/s00425-012-1698-7 ;  PMID:  22798060
• OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice .  DOI:  10.1007/s11103-012-9941-y ;  PMID:  22875749
• The WRKY Gene Family in Rice (Oryza sativa) .  DOI:  10.1111/j.1744-7909.2007.00504.x
• Functional Involvement of a Mitogen Activated Protein Kinase Module, OsMKK3-OsMPK7-OsWRK30 in Mediating Resistance against Xanthomonas oryzae in Rice .  DOI:  10.1038/srep37974 ;  PMID:  27897241
• OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak .  DOI:  10.1111/jipb.13249 ;  PMID:  35293133

Related News:

Gene Resources:

• NCBI ID: AK065518  , BK005033  , DQ298180  , HM153428
• UniProt accessions:

Sequences: