Gene Details:
- MSU gene ID: LOC_Os08g32870
- RAPdb gene ID: Os08g0424500
- Gene Symbol: OsBADH2 fgr
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- RESULTS: The results showed that multiple mutations identical to fgr allele occur in the 13 fragrant rice accessions across China; OsBADH2 is expressed constitutively, with less expression abundance in mature roots; the disrupted OsBADH2 by RNA interference leads to significantly increased 2-acetyl-1-pyrroline production.
- We edited Wx and OsBADH2 using CRISPR/Cas9 technology to produce both homozygous male sterile mutant lines and homozygous restorer mutant lines with Cas9-free.
Function-related keywords:
Literature:
- Aroma in rice: genetic analysis of a quantitative trait . DOI: 10.1007/BF00230138 ; PMID: 24162494
- OsBADH1 is possibly involved in acetaldehyde oxidation in rice plant peroxisomes . DOI: 10.1016/j.febslet.2009.10.039 ; PMID: 19850038
- Dissecting substrate specificity of two rice BADH isoforms: Enzyme kinetics, docking and molecular dynamics simulation studies . DOI: 10.1016/j.biochi.2012.04.009 ; PMID: 22534193
- Haplotype variation at Badh2, the gene determining fragrance in rice . DOI: 10.1016/j.ygeno.2012.11.010 ; PMID: 23220350
- RFLP tagging of a gene for aroma in rice . DOI: 10.1007/BF00227391 ; PMID: 24201481
- Characterization of the major fragance gene from an aromatic japonica rice and analysis of its diversity in Asian cultivated rice . DOI: 10.1007/s00122-008-0780-9 ; PMID: 18491070
- RNAi-directed downregulation of OsBADH2 results in aroma (2-acetyl-1-pyrroline) production in rice (Oryza sativa L.) . DOI: 10.1186/1471-2229-8-100 ; PMID: 18840300
- Purification, crystallization and preliminary X-ray analysis of recombinant betaine aldehyde dehydrogenase 2 (OsBADH2), a protein involved in jasmine aroma, from Thai fragrant rice (Oryza sativa L.) . DOI: 10.1107/S1744309111030971 ; PMID: 22102032
- The gene for fragrance in rice . DOI: 10.1111/j.1467-7652.2005.00131.x ; PMID: 17129318
- Badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance . DOI: 10.1105/tpc.108.058917 ; PMID: 18599581
- The origin and evolution of fragrance in rice (Oryza sativa L.) . DOI: 10.1073/pnas.0904077106 ; PMID: 19706531
- Biochemical and enzymatic study of rice BADH wild-type and mutants: an insight into fragrance in rice . DOI: 10.1007/s10930-011-9358-5 ; PMID: 21959793
- Inactivation of an aminoaldehyde dehydrogenase is responsible for fragrance in rice . DOI: 10.1007/s11103-008-9381-x ; PMID: 18704694
- Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology . DOI: 10.1111/pbi.12312 ; PMID: 25599829
- Resequencing Reveals Different Domestication Rate for BADH1 and BADH2 in Rice (Oryza sativa) . DOI: 10.1371/journal.pone.0134801 ; PMID: 26258482
- Whole genome sequencing of Oryza sativa L. cv. Seeragasamba identifies a new fragrance allele in rice . DOI: 10.1371/journal.pone.0188920 ; PMID: 29190814
- Structural insights into betaine aldehyde dehydrogenase (BADH2) from Oryza sativa explored by modeling and simulations . DOI: 10.1038/s41598-018-31204-z ; PMID: 30150624
- RNAi-mediated down regulation of BADH2 gene for expression of 2-acetyl-1-pyrroline in non-scented indica rice IR-64 (Oryza sativa L.) . DOI: 10.1007/s13205-020-2131-8 ; PMID: 32181107
- Generation of High Yielding and Fragrant Rice (Oryza sativa L.) Lines by CRISPR/Cas9 Targeted Mutagenesis of Three Homoeologs of Cytochrome P450 Gene Family and OsBADH2 and Transcriptome and Proteome Profiling of Revealed Changes Triggered by Mutations . DOI: 10.3390/plants9060788 ; PMID: 32586052
- CRISPR-Cas9-mediated editing of BADH2 gene triggered fragrance revolution in rice . DOI: 10.1111/ppl.13871 ; PMID: 36748269
- Creation of Two-Line Fragrant Glutinous Hybrid Rice by Editing the Wx and OsBADH2 Genes via the CRISPR/Cas9 System . DOI: 10.3390/ijms24010849 ; PMID: 36614293
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os08g32870.1
ACCACCGCGCCCGCGCCGCAAGGCAGCACAGAACAGAGCACTCCCTCTCCCACCACCACTCCACACCTGACACCACGCGGGCTGCCTCCATCGTCATCGATCCATCTCCGTATCTCTCACCGACCCCAAATCGCACAGCTCCAGCTCCTCCTCGATCCCCGTCGCGATCGGCGAGATGGCCACGGCGATCCCGCAGCGGCAGCTCTTCGTCGCCGGCGAGTGGCGCGCCCCCGCGCTCGGCCGCCGCCTCCCCGTCGTCAACCCCGCCACCGAGTCCCCCATCGGCGAGATCCCGGCGGGCACGGCGGAGGACGTGGACGCGGCGGTGGCGGCGGCGCGGGAGGCGCTGAAGAGGAACCGGGGCCGCGACTGGGCGCGCGCGCCGGGCGCCGTCCGGGCCAAGTACCTCCGCGCAATCGCGGCCAAGATAATCGAGAGGAAATCTGAGCTGGCTAGACTAGAGACGCTTGATTGTGGGAAGCCTCTTGATGAAGCAGCATGGGACATGGACGATGTTGCTGGATGCTTTGAGTACTTTGCAGATCTTGCAGAATCCTTGGACAAAAGGCAAAATGCACCTGTCTCTCTTCCAATGGAAAACTTTAAATGCTATCTTCGGAAAGAGCCTATCGGTGTAGTTGGGTTGATCACACCTTGGAACTATCCTCTCCTGATGGCAACATGGAAGGTAGCTCCTGCCCTGGCTGCTGGCTGTACAGCTGTACTAAAACCATCTGAATTGGCTTCCGTGACTTGTTTGGAGCTTGCTGATGTGTGTAAAGAGGTTGGTCTTCCTTCAGGTGTGCTAAACATAGTGACTGGATTAGGTTCTGAAGCCGGTGCTCCTTTGTCATCACACCCTGGTGTAGACAAGGTTGCATTTACTGGGAGTTATGAAACTGGTAAAAAGATTATGGCTTCAGCTGCTCCTATGGTTAAGCCTGTTTCACTGGAACTTGGTGGAAAAAGTCCTATAGTGGTGTTTGATGATGTTGATGTTGAAAAAGCTGTTGAGTGGACTCTCTTTGGTTGCTTTTGGACCAATGGCCAGATTTGCAGTGCAACATCGCGTCTTATTCTTCATAAAAAAATCGCTAAAGAATTTCAAGAAAGGATGGTTGCATGGGCCAAAAATATTAAGGTGTCAGATCCACTTGAAGAGGGTTGCAGGCTTGGGCCCGTTGTTAGTGAAGGACAGTATGAGAAGATTAAGCAATTTGTATCTACCGCCAAAAGCCAAGGTGCTACCATTCTGACTGGTGGGGTTAGACCCAAGCATCTGGAGAAAGGTTTCTATATTGAACCCACAATCATTACTGATGTCGATACATCAATGCAAATTTGGAGGGAAGAAGTTTTTGGTCCAGTGCTCTGTGTGAAAGAATTTAGCACTGAAGAAGAAGCCATTGAATTGGCCAACGATACTCATTATGGTCTGGCTGGTGCTGTGCTTTCCGGTGACCGCGAGCGATGCCAGAGATTAACTGAGGAGATCGATGCCGGAATTATCTGGGTGAACTGCTCGCAACCCTGCTTCTGCCAAGCTCCATGGGGCGGGAACAAGCGCAGCGGCTTTGGACGCGAGCTCGGAGAAGGGGGCATTGACAACTACCTAAGCGTCAAGCAAGTGACGGAGTACGCCTCCGATGAGCCGTGGGGATGGTACAAATCCCCTTCCAAGCTGTAATGTAATATGCGCATACGCATGACACGCCCATCTGTTCTGATCGTTTGGAATAAGAGGCCATAGTATGACGGGATGGACTTTGTGCATATCGATGTTTGATTTGCCATGTGCTGAGCCTGGAAATAAAAAAGGTTATAGTTGGAGACTTGTGAGAGTTGTACGACTTGTACTGATGGTTACGCGACAATTTGTGCATGGATAGTTTGTGTGCTTGTACCGGGGTTCTCTCCGTA
CDS Sequence
- >LOC_Os08g32870.1
ATGGCCACGGCGATCCCGCAGCGGCAGCTCTTCGTCGCCGGCGAGTGGCGCGCCCCCGCGCTCGGCCGCCGCCTCCCCGTCGTCAACCCCGCCACCGAGTCCCCCATCGGCGAGATCCCGGCGGGCACGGCGGAGGACGTGGACGCGGCGGTGGCGGCGGCGCGGGAGGCGCTGAAGAGGAACCGGGGCCGCGACTGGGCGCGCGCGCCGGGCGCCGTCCGGGCCAAGTACCTCCGCGCAATCGCGGCCAAGATAATCGAGAGGAAATCTGAGCTGGCTAGACTAGAGACGCTTGATTGTGGGAAGCCTCTTGATGAAGCAGCATGGGACATGGACGATGTTGCTGGATGCTTTGAGTACTTTGCAGATCTTGCAGAATCCTTGGACAAAAGGCAAAATGCACCTGTCTCTCTTCCAATGGAAAACTTTAAATGCTATCTTCGGAAAGAGCCTATCGGTGTAGTTGGGTTGATCACACCTTGGAACTATCCTCTCCTGATGGCAACATGGAAGGTAGCTCCTGCCCTGGCTGCTGGCTGTACAGCTGTACTAAAACCATCTGAATTGGCTTCCGTGACTTGTTTGGAGCTTGCTGATGTGTGTAAAGAGGTTGGTCTTCCTTCAGGTGTGCTAAACATAGTGACTGGATTAGGTTCTGAAGCCGGTGCTCCTTTGTCATCACACCCTGGTGTAGACAAGGTTGCATTTACTGGGAGTTATGAAACTGGTAAAAAGATTATGGCTTCAGCTGCTCCTATGGTTAAGCCTGTTTCACTGGAACTTGGTGGAAAAAGTCCTATAGTGGTGTTTGATGATGTTGATGTTGAAAAAGCTGTTGAGTGGACTCTCTTTGGTTGCTTTTGGACCAATGGCCAGATTTGCAGTGCAACATCGCGTCTTATTCTTCATAAAAAAATCGCTAAAGAATTTCAAGAAAGGATGGTTGCATGGGCCAAAAATATTAAGGTGTCAGATCCACTTGAAGAGGGTTGCAGGCTTGGGCCCGTTGTTAGTGAAGGACAGTATGAGAAGATTAAGCAATTTGTATCTACCGCCAAAAGCCAAGGTGCTACCATTCTGACTGGTGGGGTTAGACCCAAGCATCTGGAGAAAGGTTTCTATATTGAACCCACAATCATTACTGATGTCGATACATCAATGCAAATTTGGAGGGAAGAAGTTTTTGGTCCAGTGCTCTGTGTGAAAGAATTTAGCACTGAAGAAGAAGCCATTGAATTGGCCAACGATACTCATTATGGTCTGGCTGGTGCTGTGCTTTCCGGTGACCGCGAGCGATGCCAGAGATTAACTGAGGAGATCGATGCCGGAATTATCTGGGTGAACTGCTCGCAACCCTGCTTCTGCCAAGCTCCATGGGGCGGGAACAAGCGCAGCGGCTTTGGACGCGAGCTCGGAGAAGGGGGCATTGACAACTACCTAAGCGTCAAGCAAGTGACGGAGTACGCCTCCGATGAGCCGTGGGGATGGTACAAATCCCCTTCCAAGCTGTAA
Protein Sequence
- >LOC_Os08g32870.1
MATAIPQRQLFVAGEWRAPALGRRLPVVNPATESPIGEIPAGTAEDVDAAVAAAREALKRNRGRDWARAPGAVRAKYLRAIAAKIIERKSELARLETLDCGKPLDEAAWDMDDVAGCFEYFADLAESLDKRQNAPVSLPMENFKCYLRKEPIGVVGLITPWNYPLLMATWKVAPALAAGCTAVLKPSELASVTCLELADVCKEVGLPSGVLNIVTGLGSEAGAPLSSHPGVDKVAFTGSYETGKKIMASAAPMVKPVSLELGGKSPIVVFDDVDVEKAVEWTLFGCFWTNGQICSATSRLILHKKIAKEFQERMVAWAKNIKVSDPLEEGCRLGPVVSEGQYEKIKQFVSTAKSQGATILTGGVRPKHLEKGFYIEPTIITDVDTSMQIWREEVFGPVLCVKEFSTEEEAIELANDTHYGLAGAVLSGDRERCQRLTEEIDAGIIWVNCSQPCFCQAPWGGNKRSGFGRELGEGGIDNYLSVKQVTEYASDEPWGWYKSPSKL*