Information report for OsERF48;OsDRAP1
Gene Details
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Functional Descriptions
- Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance.
- When OsERF48 was overexpressed in an either a root-specific (ROX(OsERF48) ) or whole-body (OX(OsERF48) ) manner, transgenic plants showed a longer and denser root phenotype compared to the nontransgenic (NT) controls.
- Our results demonstrated that OsERF48 regulates OsCML16, a calmodulin-like protein gene that enhance root growth and drought tolerance.
- They included, OsCML16, a key gene in calcium signaling during abiotic stress, which was shown to be a direct target of OsERF48 by Chromatin Immunoprecipitation (ChIP)-qPCR analysis and a transient protoplast expression assay.
- In the present study, we report a comprehensive analysis of the transcriptome and metabolome of rice overexpressing the OsDRAP1 gene, which encodes an ERF transcription factor and was previously identified to be conferring drought tolerance.
- Phenotypic analysis showed that OsDRAP1 overexpression (OE) improved salt tolerance by increasing the survival rate under salt stress.
- OsDRAP1 affected the physiological indices such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) to enhance redox homeostasis and membrane stability in response to salt stress.
- Higher basal expression of OsDRAP1 resulted in differential expression of genes that potentially function in intrinsic salt tolerance.
Functional Keywords
- root , growth , drought , tolerance , abiotic-stress , drought-tolerance , biotic-stress , calcium , transcription-factor , salt , salt-tolerance , salt-stress , stress , homeostasis , redox-homeostasis
Literature and News
- Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance . DOI: 10.1111/pbi.12716 ; PMID: 28244201
- Comparative transcriptome and metabolome profiling reveal molecular mechanisms underlying OsDRAP1-mediated salt tolerance in rice . DOI: 10.1038/s41598-021-84638-3 ; PMID: 33664392
Gene Resources
- UniProt: A3BT06
- EMBL: AP004658, AP004691, AP008214
- AlphaFoldDB: A3BT06
- EnsemblPlants: Os08t0408500-01
- Gramene: Os08t0408500-01
- KEGG: dosa:Os08g0408500, osa:4345541
- Orthologous matrix: PAQMQFI
- InterPro: IPR001471, IPR016177, IPR036955
- PANTHER: PTHR31657, PTHR31657:SF82
- SUPFAM: SSF54171
- PROSITE: PS51032
- Gene3D: 3.30.730.10
- OrthoDB: A3BT06
- SWISS-MODEL: A3BT06
- Conserved Domain Database: cd00018
Homologs
- Brachypodium distachyon: Bradi3g35560.1.p, Bradi4g29010.1.p
- Panicum virgatum: Pavir.2KG329600.1.p
- Setaria italica: Seita.2G188000.1.p
- Setaria viridis: Sevir.2G195600.1.p, Sevir.6G146500.1.p
- Triticum aestivum: Traes_5BL_4D65B66E2.1
- Zea mays: GRMZM2G141679_P01
Sequences
cDNA Sequence
- >LOC_Os08g31580.1
ATTCCCCAAACCCAAACGACGGAGACCCACTACTCCGAGCCATCAAGAACACACACACATCCAGAAAGCCTAATCCAATCCAAAAGCCCTAATCACCCAGACTCCATCTCTGTGAGGTTTGAGAGAGGTAGGTGAGCCGTAGATCTGAGCGAGTTCTTGGGTCTTCCAGCAGGTAGATCACTTCATCTGAGGATCAATCTTTTTTTTTTTCCTTTGTTTTTGAGCTTCTGTTGGTGTACAGGACAGAGAGTTCCAGAGCCTTTTAGTTTCTGGTGTTCTGATCTGTTCTTGGTGTAAGATTATTGGTCTGATTTGGTAGCCAAGAGGGTTAATTTTTTCCACACCTCCTTGTGCTAGTTAGCTTAGCTTATACCCCCCTTGTAAAGTGATTAGTAGATCTAGAACTTCTCTTTTCGTCTGCCAGTTCTTGGATTTTGGAAAGAACAGGTGGTTTGTTATTCAGATTTTTAGGTTAGAAAAAATCCACAAAAAAAAAGATATTCGATGGCAGCTGCTATAGAAGGAAATCTGATGCGGGCGCTGGGAGAGGCTCCGTCGCCGCAGATGCAGAAGATCGCGCCGCCGCCGTTTCATCCCGGCTTGCCGCCGGCGCCGGCGAACTTCTCCTCGGCCGGAGTCCACGGGTTCCACTACATGGGCCCGGCCCAGCTCAGCCCGGCCCAGATCCAGCGCGTCCAGGCCCAACTCCACATGCAGCGGCAGGCCCAGTCGGGGCTCGGCCCGCGGGCCCAGCCCATGAAGCCCGCTTCGGCGGCTGCTCCGGCGGCGGCGGCGGCGCGGGCGCAGAAGCTGTACCGCGGCGTGCGGCAGCGGCACTGGGGCAAGTGGGTGGCGGAGATCCGGCTGCCGCGCAACCGCACCAGGCTCTGGCTCGGCACCTTCGACACCGCCGAGGAGGCGGCGCTCACCTACGACCAGGCCGCGTACCGCCTCCGCGGCGACGCGGCGCGGCTCAACTTCCCGGACAACGCCGCGTCGCGGGGCCCGCTCGACGCCGCCGTGGACGCCAAGCTCCAGGCCATCTGCGACACCATCGCCGCGTCCAAGAACGCCTCATCCAGGTCCAGGGGCGGCGCCGGCAGGGCCATGCCCATCAACGCGCCCCTGGTCGCCGCGGCGTCGTCGTCCTCCGGCTCCGACCACTCCGGCGGCGGCGACGACGGCGGCTCGGAGACGTCGTCGTCGTCTGCGGCGGCGTCGCCGCTGGCGGAGATGGAGCAGCTGGACTTCAGCGAGGTGCCGTGGGACGAGGCGGAGGGGTTCGCGCTCACCAAGTACCCGTCGTACGAGATCGACTGGGACTCGCTGCTCAACAACAATAACTAGCTCTTCTCTTCCATAGCCCGCCATTGCCGGCCGGCGCAGATGAGGTTTTAGGCATTCTGCGCGGCGGCGAGGCGATGGATTATATGTTCGTTCTTGTGTAGATCCTTTTCTTTTTGTGTTGGTTTTTTAGGTTCCGGAGGCTGCTCGCCGGCGTCGTTTTTGTGTCCGGCGTCTCCGATGTCTAGTAAGCAGTGACTCGTTAGTGTAGTAGTTGTACAACTCTACAAATGTACAAATACTTGTGCATGTAGGTTGTTGTAATCAATTGTTGGTAACTACCTGTAATAGAACTACTGTGAATTAACTGTATTAATGTGCAAATCTTCATCGATCTTTCAGTTGTAAAACTTTGACTTCTT
CDS Sequence
- >LOC_Os08g31580.1
ATGGCAGCTGCTATAGAAGGAAATCTGATGCGGGCGCTGGGAGAGGCTCCGTCGCCGCAGATGCAGAAGATCGCGCCGCCGCCGTTTCATCCCGGCTTGCCGCCGGCGCCGGCGAACTTCTCCTCGGCCGGAGTCCACGGGTTCCACTACATGGGCCCGGCCCAGCTCAGCCCGGCCCAGATCCAGCGCGTCCAGGCCCAACTCCACATGCAGCGGCAGGCCCAGTCGGGGCTCGGCCCGCGGGCCCAGCCCATGAAGCCCGCTTCGGCGGCTGCTCCGGCGGCGGCGGCGGCGCGGGCGCAGAAGCTGTACCGCGGCGTGCGGCAGCGGCACTGGGGCAAGTGGGTGGCGGAGATCCGGCTGCCGCGCAACCGCACCAGGCTCTGGCTCGGCACCTTCGACACCGCCGAGGAGGCGGCGCTCACCTACGACCAGGCCGCGTACCGCCTCCGCGGCGACGCGGCGCGGCTCAACTTCCCGGACAACGCCGCGTCGCGGGGCCCGCTCGACGCCGCCGTGGACGCCAAGCTCCAGGCCATCTGCGACACCATCGCCGCGTCCAAGAACGCCTCATCCAGGTCCAGGGGCGGCGCCGGCAGGGCCATGCCCATCAACGCGCCCCTGGTCGCCGCGGCGTCGTCGTCCTCCGGCTCCGACCACTCCGGCGGCGGCGACGACGGCGGCTCGGAGACGTCGTCGTCGTCTGCGGCGGCGTCGCCGCTGGCGGAGATGGAGCAGCTGGACTTCAGCGAGGTGCCGTGGGACGAGGCGGAGGGGTTCGCGCTCACCAAGTACCCGTCGTACGAGATCGACTGGGACTCGCTGCTCAACAACAATAACTAG
Protein Sequence
- >LOC_Os08g31580.1
MAAAIEGNLMRALGEAPSPQMQKIAPPPFHPGLPPAPANFSSAGVHGFHYMGPAQLSPAQIQRVQAQLHMQRQAQSGLGPRAQPMKPASAAAPAAAAARAQKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALTYDQAAYRLRGDAARLNFPDNAASRGPLDAAVDAKLQAICDTIAASKNASSRSRGGAGRAMPINAPLVAAASSSSGSDHSGGGDDGGSETSSSSAAASPLAEMEQLDFSEVPWDEAEGFALTKYPSYEIDWDSLLNNNN*