Gene Details:
- MSU gene ID: LOC_Os08g31460
- RAPdb gene ID: Os08g0407000
- Gene Symbol: PDD TCD8
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- LC-MS/MS revealed that PDD is associated with the 5-methylaminomethyl-2-thiouridine modification of chloroplast tRNA.
- Map-based cloning revealed that PDD encodes an evolutionarily conserved tRNA-modifying GTPase belonging to the TrmE family.
- Rice TCD8 Encoding a Multi-Domain GTPase Is Crucial for Chloroplast Development of Early Leaf Stage at Low Temperatures.
- In addition, the disturbance of TCD8 suppressed the transcripts of certain chloroplast-related genes at low temperature, although the genes were recoverable to nearly normal levels at high temperature (32 °C), indicating that TCD8 governs chloroplast development at low temperature.
- Map-based cloning and complementary test revealed the TCD8 encoded a multi-domain GTPase localized in chloroplasts.
Function-related keywords:
Literature:
- A Natural Variation in PLEIOTROPIC DEVELOPMENTAL DEFECTS Uncovers a Crucial Role for Chloroplast tRNA Modification in Translation and Plant Development . DOI: 10.1105/tpc.19.00660 ; PMID: 32327539
- Rice TCD8 Encoding a Multi-Domain GTPase Is Crucial for Chloroplast Development of Early Leaf Stage at Low Temperatures . DOI: 10.3390/biology11121738 ; PMID: 36552248
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os08g31460.1
ATGGCTCGCGCTCTCTCTCGCCTCCTCCTCCCGCCCCTCTTCCTATGCCGCCCTCTCCCACTCCCACCCCTCCTCCACCGCCGCCGCCGCGTCGTCCTAAACCCTAGCCCCAGAACCCTCACCACGCGCGCCGAGGCCCTCTTCTCCCGCCACTCCCACCTCCTCCCCGCCGACGACCGCTCCCCCTCCCCCTCCACCCGCGGCTCCGTCCAGCCGCCGCCGGACTATGGCGGCGGCGGAGGCGGAGCGGGCCCCGGGACCATCGCCGCCATCGTCACCTCCCTCGGCGGCGGCCCCGCGGCGGTGGGGATCGTGCGGCTATCCGGCCCCGACGCGGCCGCCGTCGCGGGGCGCGTGTTCCGGCCGGCCAGGAGGGCGGCGCCGCCGTGGCGGCCGCGCAGCCATTTCGTCGAGTACGGGGTCGCGCTCGACCGCGATGGCGGCGTCATCGACGAGGTCTTAGTGGTGCCGATGCTAGCTCCGAGATCGTACACGCGGGAGGATGTGGTGGAGCTCCAGTGCCATGGGAATGACCTGTGCTTGCGGCGTGTGCTCAGGGCCTGCTTGGAAGCTGGTGCTAGGCTTGCTGACCCTGGTGAGTTCACTGTTCGTGCGTTTCTGAATGGGAGGTTGGACTTGGCACAAGCGGAGAATGTGTCAAGGCTCATTTCAGCCAAATCTGCAGCTGCGGCAGACTCAGCATTGGCTGGCATACAGGGAGGCTTTTCTGCACTAGTAAAATCACTGAGATCAAGGTGCATAGAATTGCTCACTGAGATTGAGGCCCGTCTAGACTTTGAAGATGAATTGCCTCCTTTGGATCTAACAATGCTGGTCAGCAAAATTAATGGTATGAGACAAGAGGTTCAGGATGCTTTGGACACTGCTAATTATGATAAGCTGCTACAATCTGGTCTGCAGGTAGCAATAATTGGCCGCCCAAATGTTGGCAAGTCAAGCCTGCTGAATGCTTGGAGTAAGAGTGAAAGGGCTATTGTTACTGAAATAGCTGGAACCACAAGGGATGTAGTAGAAGCAAATGTCTCAATTCATGGCATCCCTATTACCCTTCTAGATACTGCTGGTATAAGAGAAACTGATGACATCGTGGAAAAAATTGGTGAGATATTATCTTGGAAATATTCAATGCTGCACAGTGCATACTTGTATAATGGTGTGGAACGATCAGAAGCTGCTGCACTGGGAGCTGATCTGATTATTATGACAATAAGTGCAGTTGATGGGTGGACTGAAGATGACACTAAACTTATTGAGCATGTGTTGATCAGTAAGAAATCTTCAGGTGCTCCAGTGCCAATGGTCCTTGTAATCAACAAGGTAGATTGTGCACCATTTATTTCAGGAGAACAGTTTGAACAATTCCATGGAGTTTTCAAAAAACATGTGCAAACATGTGCAGTCACTGGTAAAGGCATTTCTGAGTTGGAGAAGGCTATAATAGAGGTCAGAGGTCTTGAGCCTGTACCATCAGAGGGACGGCGATGGACTGTAAATCAGAGGCAATTTGAACAGCTATTGAGGACCCAACAAGCTTTTACTAGGCTAGAGTCATCTATCAACGAGCAGTTGCCGATGGACTTCTGGACTATAGACTTGAGGGAAGCAGCATTGGCTCTTGCAACAATCAGCGGGGAGGATATCTCTGAAGAAGTCCTGAGCAGCATATTCAGCAAATTTTGCATTGGAAAATGACTGGCAAGCACGGCAGTAACATATATCGGCGCATAAAATTATGTGAATTGCCAGTCAGTCACCATCCGACTCATGTTTGAGACACAACAGGAGTACATGACTTAGTTTGCTCGAACATATTGATGATTGAGCTCAATGACTGGCTTCGAGTTTACTAGATTTTTCACCGTTCACTTGAGCAGCTGTGCTATATATGCTTGCAAAACTTGCCTCATCTACACGAGCAGTTTAGAGGCAGTTTATAGCTCTGTAATGGAAGAAGACTCATATGTACTGCTTCGATCATCCTCACAACTGTTTTGAATGACTGCCACAATGTATGGATATGCCTAGTGCTTGTGATTGGCAATTGTGCTTTGGTCGCGCATCTAATGGCAGACGGCTTGACCC
CDS Sequence
- >LOC_Os08g31460.1
ATGGCTCGCGCTCTCTCTCGCCTCCTCCTCCCGCCCCTCTTCCTATGCCGCCCTCTCCCACTCCCACCCCTCCTCCACCGCCGCCGCCGCGTCGTCCTAAACCCTAGCCCCAGAACCCTCACCACGCGCGCCGAGGCCCTCTTCTCCCGCCACTCCCACCTCCTCCCCGCCGACGACCGCTCCCCCTCCCCCTCCACCCGCGGCTCCGTCCAGCCGCCGCCGGACTATGGCGGCGGCGGAGGCGGAGCGGGCCCCGGGACCATCGCCGCCATCGTCACCTCCCTCGGCGGCGGCCCCGCGGCGGTGGGGATCGTGCGGCTATCCGGCCCCGACGCGGCCGCCGTCGCGGGGCGCGTGTTCCGGCCGGCCAGGAGGGCGGCGCCGCCGTGGCGGCCGCGCAGCCATTTCGTCGAGTACGGGGTCGCGCTCGACCGCGATGGCGGCGTCATCGACGAGGTCTTAGTGGTGCCGATGCTAGCTCCGAGATCGTACACGCGGGAGGATGTGGTGGAGCTCCAGTGCCATGGGAATGACCTGTGCTTGCGGCGTGTGCTCAGGGCCTGCTTGGAAGCTGGTGCTAGGCTTGCTGACCCTGGTGAGTTCACTGTTCGTGCGTTTCTGAATGGGAGGTTGGACTTGGCACAAGCGGAGAATGTGTCAAGGCTCATTTCAGCCAAATCTGCAGCTGCGGCAGACTCAGCATTGGCTGGCATACAGGGAGGCTTTTCTGCACTAGTAAAATCACTGAGATCAAGGTGCATAGAATTGCTCACTGAGATTGAGGCCCGTCTAGACTTTGAAGATGAATTGCCTCCTTTGGATCTAACAATGCTGGTCAGCAAAATTAATGGTATGAGACAAGAGGTTCAGGATGCTTTGGACACTGCTAATTATGATAAGCTGCTACAATCTGGTCTGCAGGTAGCAATAATTGGCCGCCCAAATGTTGGCAAGTCAAGCCTGCTGAATGCTTGGAGTAAGAGTGAAAGGGCTATTGTTACTGAAATAGCTGGAACCACAAGGGATGTAGTAGAAGCAAATGTCTCAATTCATGGCATCCCTATTACCCTTCTAGATACTGCTGGTATAAGAGAAACTGATGACATCGTGGAAAAAATTGGTGAGATATTATCTTGGAAATATTCAATGCTGCACAGTGCATACTTGTATAATGGTGTGGAACGATCAGAAGCTGCTGCACTGGGAGCTGATCTGATTATTATGACAATAAGTGCAGTTGATGGGTGGACTGAAGATGACACTAAACTTATTGAGCATGTGTTGATCAGTAAGAAATCTTCAGGTGCTCCAGTGCCAATGGTCCTTGTAATCAACAAGGTAGATTGTGCACCATTTATTTCAGGAGAACAGTTTGAACAATTCCATGGAGTTTTCAAAAAACATGTGCAAACATGTGCAGTCACTGGTAAAGGCATTTCTGAGTTGGAGAAGGCTATAATAGAGGTCAGAGGTCTTGAGCCTGTACCATCAGAGGGACGGCGATGGACTGTAAATCAGAGGCAATTTGAACAGCTATTGAGGACCCAACAAGCTTTTACTAGGCTAGAGTCATCTATCAACGAGCAGTTGCCGATGGACTTCTGGACTATAGACTTGAGGGAAGCAGCATTGGCTCTTGCAACAATCAGCGGGGAGGATATCTCTGAAGAAGTCCTGAGCAGCATATTCAGCAAATTTTGCATTGGAAAATGA
Protein Sequence
- >LOC_Os08g31460.1
MARALSRLLLPPLFLCRPLPLPPLLHRRRRVVLNPSPRTLTTRAEALFSRHSHLLPADDRSPSPSTRGSVQPPPDYGGGGGGAGPGTIAAIVTSLGGGPAAVGIVRLSGPDAAAVAGRVFRPARRAAPPWRPRSHFVEYGVALDRDGGVIDEVLVVPMLAPRSYTREDVVELQCHGNDLCLRRVLRACLEAGARLADPGEFTVRAFLNGRLDLAQAENVSRLISAKSAAAADSALAGIQGGFSALVKSLRSRCIELLTEIEARLDFEDELPPLDLTMLVSKINGMRQEVQDALDTANYDKLLQSGLQVAIIGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVVEANVSIHGIPITLLDTAGIRETDDIVEKIGEILSWKYSMLHSAYLYNGVERSEAAALGADLIIMTISAVDGWTEDDTKLIEHVLISKKSSGAPVPMVLVINKVDCAPFISGEQFEQFHGVFKKHVQTCAVTGKGISELEKAIIEVRGLEPVPSEGRRWTVNQRQFEQLLRTQQAFTRLESSINEQLPMDFWTIDLREAALALATISGEDISEEVLSSIFSKFCIGK*