Gene Details:

Functional Descriptions:

  • The Rice BZ1 Locus Is Required for Glycosylation of Arabinogalactan Proteins and Galactolipid and Plays a Role in both Mechanical Strength and Leaf Color.
  • Molecular and biochemical analysis revealed that BZ1 encodes a functional UDP-galactose/glucose epimerase (UGE) and is ubiquitously expressed with higher expression in stem and leaf tissues.
  • Lipid profiling assay showed that the amount of monogalactosyldiacylglycerols (MGDG), a major chloroplast membrane glycolipid, was significantly decreased in BZ1.
  • Taken together, these results strongly demonstrate that BZ1 participates in UDP-galactose supply for the sugar chains biosynthesis of AGPs and MGDG, which thereby, respectively, results in altered cell wall and abnormal chloroplast development.
  • Due to inferior mechanical strength and reduced photosynthesis, BZ1 plants displayed detrimental agronomic traits, whereas BZ1 overexpressing lines showed enhanced plant growth.
  • Transcriptome analysis of stems and leaves further showed that numerous key genes involved in AGPs biosynthesis and photosynthesis metabolism were substantially suppressed in BZ1.
  • CONCLUSIONS: Our finding identifies BZ1 as a dual-targeting UGE protein for glycosylation of AGPs and MGDG and suggests a strategy for breeding robust elite crops.
  • Multiple techniques analyses, including immunoblots, immuno-gold, and cryogenic scanning electron microscopy, demonstrated a significantly impaired glycosylation of arabinogalactan proteins (AGPs) and disordered cellulose microfibril deposition in BZ1.
  • The Rice BZ1 Locus Is Required for Glycosylation of Arabinogalactan Proteins and Galactolipid and Plays a Role in both Mechanical Strength and Leaf Color.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os08g28730.1
    AACTCCTCTTCCCTGAATCCCTCCTCCTACTCTTCTCCTCCCCACATTCCCAAAGAGAAGCTTCGCGGCCTCCTCCTCCTCCACCTCCTCCGCATCCTCCTCCGAGCCCCCAGATCCGCCGCGCGTGCTCTCGCGGGTGGTGGTGCTTCTGGTCTTGGGGGAGGGGAGGGGCGCGAGCGAGCGAGCGAACGAGCGAGAGAGAGAGAGAGAGAGAGAGGAAGGGGAGGGAGAGGGAGGTGTGTGTGTGTGAGAGAGAGAGAGATGGCGGTGGAGAAGACGGTGCCGGGCGGGGTGAGGACGGTGCTGGTGACGGGCGGGGCGGGGTACATCGGCAGCCACGCCGTGCTGCAGCTCCTCCTCGCCGGATTCCGCGCCGTCGTCGTCGACAACCTCAACAACTCCTCCGAGCTCGCCGTCCGCCGCGTCGCCGCCCTCGCCGGCGACCACTCCCGGAACCTCGCCTTCCACAAGGTTGACCTCCGTGACAAGGGAGCACTGGAAAAGGTTTTTGCTTCAACAAGATTCGATGCTGTAGTTCACTTTGCTGGGCTAAAAGCTGTGGGCGAAAGTGTGCAGAAGCCATTGCTTTATTACGACAACAATGTTAATGGAACAGTAAATCTTCTGGAAGTTATGTCTGCCCATGGATGTAAGAAGTTGGTGTTCTCATCATCAGCTGCAGTTTATGGATCACCGAAGAACTCACCCTGCACAGAAGAGTTTCCTCTCACTCCAAACAATCCATATGGCAAAACAAAGCTTGTTGTTGAAGATATTTGCCGTGATATCTACCGTACAGATCCTGAATGGAAGATTATTCTACTTAGGTACTTCAATCCAGTTGGGGCTCATCCTAGCGGATACCTTGGGGAAGATCCATGTGGCATTCCCAACAATCTTATGCCTTATGTTCAGCAAGTTGCTGTTGGCAGGAGGCCAGCTCTGACAATACTAGGAAATGATTATGCAACCAGAGATGGTACCGGGGTCCGAGATTACATCCATGTGGTTGACCTTGCTGATGGACATATTGCGGCATTGCAGAAACTCTTTGAGAGCTCCAGCATAGGGTGTGAAGCATACAACCTTGGAACTGGTAAAGGTACCTCTGTGCTGGAGATAGTCAAGGCATTTGAGAAGGCTTCTGGGAAGAAAATTCCTCTGATTATTGGCCCAAGACGCCCTGGTGATGCGGAGATTCTATTTTCATTACCTGCTAAAGCAGAGAAGGAACTCAACTGGAAAGCGAAATTTGGCATCGATGAAATGTGTAGGGATCAATGGAACTGGGCTAGCAAGAACCCTTATGGATACGGGTCACTTGACTCCACCAAGCAGAACGGACACCACTCGTACGGATCAATTGGCTCTCCCAAGCAGAATGGCCACTGCACAAATGGATTTTCTGAATCCACTAGGCATAATGGTCACAACGGATATGGACTGGTCGACTCTGCCAAGCATAACGGCAACGGCCACTTCCACTGACCATGCTTCAGCCTTGAGTTGGCCTGTGGGCTCCTTGTACATGTGATGTATCGAAACGATGATCCGAGATCGTTGCAGGTCAACGATGGATTGGCAGGTCACACGAACATGGTCGAGCTTAGATAATATTCTTTCATTGTTCATCGTTGGCTGAAGAATCCCACTGCCTGTGGGTATATATTTATCTCCTACTTTGAGTGTATTATTTTTCTTTCAGATTTGTTGTAGGAATGTGACATTTAGCCAACCAGCTGATTCGATTCTTTCACTGCTAATTGAGCTGCATTATCTGTTACAGAGCTTTGACTGATTCCTGTACCATATATCTCTTTTTGTGTATTTTATGATGTAATATTTATGAAGGGGAATTAGTCTAAAACCGATGCTCATGTTTTCTCTTCACATGTCATATGTTCATTCGTGGAAGTGGACAGCCCAAGTACTATCCATGCGGATGAGAAATAGAAATTCTATTTCAGATAAAAGCTTTTCTTTTGTGTAATTCTTGCGTGTGGCTCAATTCTGTTAGTGTCAATATGCCATTTGGGACAAATGCACGTTAGACGAGCGCATTTGACTTC
CDS Sequence
  • >LOC_Os08g28730.1
    ATGGCGGTGGAGAAGACGGTGCCGGGCGGGGTGAGGACGGTGCTGGTGACGGGCGGGGCGGGGTACATCGGCAGCCACGCCGTGCTGCAGCTCCTCCTCGCCGGATTCCGCGCCGTCGTCGTCGACAACCTCAACAACTCCTCCGAGCTCGCCGTCCGCCGCGTCGCCGCCCTCGCCGGCGACCACTCCCGGAACCTCGCCTTCCACAAGGTTGACCTCCGTGACAAGGGAGCACTGGAAAAGGTTTTTGCTTCAACAAGATTCGATGCTGTAGTTCACTTTGCTGGGCTAAAAGCTGTGGGCGAAAGTGTGCAGAAGCCATTGCTTTATTACGACAACAATGTTAATGGAACAGTAAATCTTCTGGAAGTTATGTCTGCCCATGGATGTAAGAAGTTGGTGTTCTCATCATCAGCTGCAGTTTATGGATCACCGAAGAACTCACCCTGCACAGAAGAGTTTCCTCTCACTCCAAACAATCCATATGGCAAAACAAAGCTTGTTGTTGAAGATATTTGCCGTGATATCTACCGTACAGATCCTGAATGGAAGATTATTCTACTTAGGTACTTCAATCCAGTTGGGGCTCATCCTAGCGGATACCTTGGGGAAGATCCATGTGGCATTCCCAACAATCTTATGCCTTATGTTCAGCAAGTTGCTGTTGGCAGGAGGCCAGCTCTGACAATACTAGGAAATGATTATGCAACCAGAGATGGTACCGGGGTCCGAGATTACATCCATGTGGTTGACCTTGCTGATGGACATATTGCGGCATTGCAGAAACTCTTTGAGAGCTCCAGCATAGGGTGTGAAGCATACAACCTTGGAACTGGTAAAGGTACCTCTGTGCTGGAGATAGTCAAGGCATTTGAGAAGGCTTCTGGGAAGAAAATTCCTCTGATTATTGGCCCAAGACGCCCTGGTGATGCGGAGATTCTATTTTCATTACCTGCTAAAGCAGAGAAGGAACTCAACTGGAAAGCGAAATTTGGCATCGATGAAATGTGTAGGGATCAATGGAACTGGGCTAGCAAGAACCCTTATGGATACGGGTCACTTGACTCCACCAAGCAGAACGGACACCACTCGTACGGATCAATTGGCTCTCCCAAGCAGAATGGCCACTGCACAAATGGATTTTCTGAATCCACTAGGCATAATGGTCACAACGGATATGGACTGGTCGACTCTGCCAAGCATAACGGCAACGGCCACTTCCACTGA
Protein Sequence
  • >LOC_Os08g28730.1
    MAVEKTVPGGVRTVLVTGGAGYIGSHAVLQLLLAGFRAVVVDNLNNSSELAVRRVAALAGDHSRNLAFHKVDLRDKGALEKVFASTRFDAVVHFAGLKAVGESVQKPLLYYDNNVNGTVNLLEVMSAHGCKKLVFSSSAAVYGSPKNSPCTEEFPLTPNNPYGKTKLVVEDICRDIYRTDPEWKIILLRYFNPVGAHPSGYLGEDPCGIPNNLMPYVQQVAVGRRPALTILGNDYATRDGTGVRDYIHVVDLADGHIAALQKLFESSSIGCEAYNLGTGKGTSVLEIVKAFEKASGKKIPLIIGPRRPGDAEILFSLPAKAEKELNWKAKFGIDEMCRDQWNWASKNPYGYGSLDSTKQNGHHSYGSIGSPKQNGHCTNGFSESTRHNGHNGYGLVDSAKHNGNGHFH*