Gene Details:

Functional Descriptions:

  • Overexpression of a New Zinc Finger Protein Transcription Factor OsCTZFP8 Improves Cold Tolerance in Rice.
  • In the present study, we isolated and functionally characterized a new C2H2 zinc finger protein transcription factor OsCTZFP8 in rice.
  • These results demonstrate that OsCTZFP8 was a C2H2 zinc finger transcription factor that plays an important role in cold tolerance in rice.
  • Transgenic rice overexpressing OsCTZFP8 exhibited cold tolerant phenotypes with significantly higher pollen fertilities and seed setting rates than nontransgenic control plants.
  • Expression of OsCTZFP8 was differentially induced by several abiotic stresses and was strongly induced by cold stress.
  • OsCTZFP8 encodes a C2H2 zinc finger protein, which contains a typical zinc finger motif, as well as a potential nuclear localization signal (NLS) and a leucine-rich region (L-box).
  • Subcellular localization assay and yeast one-hybrid analysis revealed that OsCTZFP8 was a nuclear protein and has transactivation activity.
  • To characterize the function of OsCTZFP8 in rice, the full-length cDNA of OsCTZFP8 was isolated and transgenic rice with overexpression of OsCTZFP8 driven by the maize ubiquitin promoter was generated using Agrobacterium-mediated transformation.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os08g20580.1
    ATGGCGATGGCATTTTTGGGACAAAGTCGTTTGTACGATGGCATTTCTTGGAACTCACACTTGTCGATGGCATTTCTGGTCCCACCTGTCAGCGTGGCCACGTCAGCACCCTCTCTCTCTCTTCCCCCTCCTCTCCCTTCCTCATCTCTCTCTCTCTCACTCTTCTCGGCAAGCCGGCCGGTGGCTGGGGCGAAGGCCGCCAGGGTGAGGCGGCGGCGGCAGGTCGCGAATGGCGAGACGGAAGCGCTCCACGCCGCGGTGCTCAAGGAGGAGGAGCAGCAGCACGAGGTGGAGGAGGCGGCGGTCGTGACGAGCAGCAGCGCCACAAGCGGGGAGGAGGGCGGGCACCTACCGCAGGGGTGGGCGAAGCGGAAGAGGTCGCGCCGCCAGCGATCGGAGGAGGAGAACCTCGCGCTCTGCCTTCTCATGCTCGCCCTCGGCGGCCACCACCGCGTCCAGGCGCCGCCTCCTCTCTCGGCGCCGGTAGGTGCGGAGTTCAAGTGCTCCGTCTGCGGCAGGTCCTTCAGCTCCTACCAGGCGCTCGGCGGCCACAAGACGAGCCACCGGTTCAAGCTGCCTACTCCGCCCGCATCTCCCGTCTTGGCTCCTGCCTCCTCCGAGGTCCAGAGCCCCCTCGCCTTCTCACCGCGTAATTCAGCAGCTGCACGGATCTGA
CDS Sequence
  • >LOC_Os08g20580.1
    ATGGCGATGGCATTTTTGGGACAAAGTCGTTTGTACGATGGCATTTCTTGGAACTCACACTTGTCGATGGCATTTCTGGTCCCACCTGTCAGCGTGGCCACGTCAGCACCCTCTCTCTCTCTTCCCCCTCCTCTCCCTTCCTCATCTCTCTCTCTCTCACTCTTCTCGGCAAGCCGGCCGGTGGCTGGGGCGAAGGCCGCCAGGGTGAGGCGGCGGCGGCAGGTCGCGAATGGCGAGACGGAAGCGCTCCACGCCGCGGTGCTCAAGGAGGAGGAGCAGCAGCACGAGGTGGAGGAGGCGGCGGTCGTGACGAGCAGCAGCGCCACAAGCGGGGAGGAGGGCGGGCACCTACCGCAGGGGTGGGCGAAGCGGAAGAGGTCGCGCCGCCAGCGATCGGAGGAGGAGAACCTCGCGCTCTGCCTTCTCATGCTCGCCCTCGGCGGCCACCACCGCGTCCAGGCGCCGCCTCCTCTCTCGGCGCCGGTAGGTGCGGAGTTCAAGTGCTCCGTCTGCGGCAGGTCCTTCAGCTCCTACCAGGCGCTCGGCGGCCACAAGACGAGCCACCGGTTCAAGCTGCCTACTCCGCCCGCATCTCCCGTCTTGGCTCCTGCCTCCTCCGAGGTCCAGAGCCCCCTCGCCTTCTCACCGCGTAATTCAGCAGCTGCACGGATCTGA
Protein Sequence
  • >LOC_Os08g20580.1
    MAMAFLGQSRLYDGISWNSHLSMAFLVPPVSVATSAPSLSLPPPLPSSSLSLSLFSASRPVAGAKAARVRRRRQVANGETEALHAAVLKEEEQQHEVEEAAVVTSSSATSGEEGGHLPQGWAKRKRSRRQRSEEENLALCLLMLALGGHHRVQAPPPLSAPVGAEFKCSVCGRSFSSYQALGGHKTSHRFKLPTPPASPVLAPASSEVQSPLAFSPRNSAAARI*