Information report for COMT;OsCOMT
Gene Details
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Functional Descriptions
- We propose that PAL, COMT, and GSTU17 are likely to confer PBZ-induced disease resistance via such functions as biosynthesis and transport of flavonoid-type phytoalexin and/or lignin biogenesis.
- Similar to AtCOMT, OsCOMT was localized in the cytoplasm.
- Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice.
- OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls.
- These data establish OsCAldOMT1 as a bifunctional O-methyltransferase predominantly involved in the two parallel metabolic pathways both dedicated to the biosynthesis of tricin-lignins in rice cell walls.
- Transgenic assays suggested that OsCOMT significantly delays leaf senescence at the grain filling stage by inhibiting degradation of chlorophyll and chloroplast, which, in turn, improves photosynthesis efficiency.
- In addition, the number and size of vascular bundles in the culms and leaves were significantly increased in the OsCOMT-overexpressing plants, while decreased in the knockout plants, suggesting that OsCOMT plays a positive role in vascular development of rice.
- More importantly, we found that OsCOMT is a positive regulator of grain yield, and overexpression of OsCOMT increase grain yield per plant even in a high-yield variety background, suggesting that OsCOMT can be used as an important target for enhancing rice yield.
- In vitro and in vivo evidence revealed that OsCOMT is involved in melatonin biosynthesis.
- Further, OsCAldOMT1 deficiency contributed to the increase of the cellulose molecular mobility more prominently than OsCAD2 deficiency, resulting in apparently more loosened lignocellulose molecular assembly.
- A set of rice mutants harboring knockout mutations in either or both OsCAldOMT1 and OsCAD2 was generated in part by genome editing and subjected to comparative cell wall chemical and supramolecular structure analyses.
Functional Keywords
Literature and News
- OsCAD2 is the major CAD gene responsible for monolignol biosynthesis in rice culm . DOI: 10.1007/s00299-011-1142-7 ; PMID: 21912859
- Flavonoid 3’-O-methyltransferase from rice: cDNA cloning, characterization and functional expression . DOI: 10.1016/j.phytochem.2005.11.022 ; PMID: 16412485
- Proteomic analysis of rice defense response induced by probenazole . DOI: 10.1016/j.phytochem.2007.09.005 ; PMID: 17950386
- Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice . DOI: 10.1093/jxb/erv396 ; PMID: 26276868
- OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls . DOI: 10.1038/s41598-019-47957-0 ; PMID: 31406182
- OsCOMT, encoding a caffeic acid O-methyltransferase in melatonin biosynthesis, increases rice grain yield through dual regulation of leaf senescence and vascular development . DOI: 10.1111/pbi.13794 ; PMID: 35189026
- Genetic modification of flavone biosynthesis in rice enhances biofilm formation of soil diazotrophic bacteria and biological nitrogen fixation . DOI: 10.1111/pbi.13894 ; PMID: 35869808
- Lignocellulose molecular assembly and deconstruction properties of lignin-altered rice mutants . DOI: 10.1093/plphys/kiac432 ; PMID: 36124989
Gene Resources
- UniProt: Q6ZD89
- EMBL: AB122056, AK064768, AP004460
- AlphaFoldDB: Q6ZD89
- EnsemblPlants: Os08t0157500-01
- Gramene: Os08t0157500-01
- KEGG: dosa:Os08g0157500
- Orthologous matrix: YAMVKCN
- InterPro: IPR001077, IPR012967, IPR016461
- PANTHER: PTHR11746
- SUPFAM: SSF46785, SSF53335
- PROSITE: PS51683
- Gene3D: 1.10.10.10, 3.40.50.150
- OrthoDB: Q6ZD89
- SWISS-MODEL: Q6ZD89
- eggNOG: KOG3178
Sequences
cDNA Sequence
- >LOC_Os08g06100.1
GAGCTCCCATATAAACCCCCTCCTCCCTCTCCATTGCCTTCACCTCACTCGCCGCCGGTGATCAGCCTCCTCCTCCTTCTTCTCCATCGCCGGCGAGAGAGAGAGAGAGTTAGCTAGCTAGGATGGGTTCTACAGCCGCCGACATGGCCGCGGCGGCCGACGAGGAGGCGTGCATGTACGCGCTGCAGCTGGCGTCGTCGTCGATCCTGCCGATGACGCTCAAGAACGCCATCGAGCTGGGCCTGCTCGAGACGCTGCAGTCCGCCGCCGTCGCCGGAGGAGGGGGGAAGGCGGCGCTGCTGACGCCGGCGGAGGTGGCCGACAAGCTGCCGTCCAAGGCGAACCCGGCGGCGGCCGACATGGTGGACCGCATGCTCCGCCTGCTCGCCTCCTACAACGTCGTCAGGTGCGAGATGGAGGAGGGCGCCGACGGCAAGCTCTCCCGCCGCTACGCCGCCGCGCCGGTGTGCAAGTGGCTGACGCCCAACGAGGACGGCGTCTCCATGGCCGCCCTCGCCCTCATGAACCAGGACAAGGTCCTCATGGAGAGCTGGTACTACCTTAAGGACGCAGTCCTGGACGGCGGCATCCCGTTCAACAAGGCGTACGGGATGACGGCGTTCGAGTACCACGGCACGGACGCCCGCTTCAACCGCGTCTTCAACGAGGGCATGAAGAACCACTCCGTCATCATCACCAAGAAGCTGCTCGACCTCTACACCGGCTTCGACGCCGCCTCCACCGTCGTCGACGTCGGCGGCGGCGTGGGCGCCACTGTGGCCGCCGTCGTCTCCCGCCACCCGCACATCCGGGGGATCAACTACGACCTCCCCCACGTCATCTCCGAGGCGCCGCCGTTCCCCGGGGTGGAGCACGTCGGCGGCGACATGTTCGCCTCCGTGCCCCGCGGCGGCGACGCCATCCTGATGAAGTGGATCCTCCACGACTGGAGCGACGAGCACTGCGCGCGGCTGCTCAAGAACTGCTACGACGCGCTGCCGGAGCACGGGAAGGTGGTGGTGGTGGAGTGCGTGCTGCCGGAGAGCTCCGACGCGACGGCGAGGGAGCAGGGGGTGTTCCACGTCGACATGATCATGCTCGCCCACAACCCCGGCGGCAAGGAGAGGTACGAGAGGGAGTTCAGGGAGCTCGCCCGCGCCGCCGGATTCACCGGCTTCAAGGCCACCTACATCTACGCCAACGCCTGGGCCATCGAGTTCACCAAGTAGGTGATTGGTGATCGATCGCCATTGTTGAGCTCGATCAAGGTGTTCGACCATCGTCTTCTTCTTCTCGTACTTCTTGTTCTTCATCCAAAAGCGTGGGTAATCATGTCGTTTGCTGCTGGCTGATGCTGCTGCTGCTTGTGTTGTACTTTTGATGTTCAATTCCGGTGATTCTGAGTTCTAATGGATGTAACCTGTCTGCTATTATATATATATATAATCGAATATATGATATATTTACTTACGACTTGTTAATGATTCACAAGGTTATATAATTTCTTCTCAATCAATC
CDS Sequence
- >LOC_Os08g06100.1
ATGGGTTCTACAGCCGCCGACATGGCCGCGGCGGCCGACGAGGAGGCGTGCATGTACGCGCTGCAGCTGGCGTCGTCGTCGATCCTGCCGATGACGCTCAAGAACGCCATCGAGCTGGGCCTGCTCGAGACGCTGCAGTCCGCCGCCGTCGCCGGAGGAGGGGGGAAGGCGGCGCTGCTGACGCCGGCGGAGGTGGCCGACAAGCTGCCGTCCAAGGCGAACCCGGCGGCGGCCGACATGGTGGACCGCATGCTCCGCCTGCTCGCCTCCTACAACGTCGTCAGGTGCGAGATGGAGGAGGGCGCCGACGGCAAGCTCTCCCGCCGCTACGCCGCCGCGCCGGTGTGCAAGTGGCTGACGCCCAACGAGGACGGCGTCTCCATGGCCGCCCTCGCCCTCATGAACCAGGACAAGGTCCTCATGGAGAGCTGGTACTACCTTAAGGACGCAGTCCTGGACGGCGGCATCCCGTTCAACAAGGCGTACGGGATGACGGCGTTCGAGTACCACGGCACGGACGCCCGCTTCAACCGCGTCTTCAACGAGGGCATGAAGAACCACTCCGTCATCATCACCAAGAAGCTGCTCGACCTCTACACCGGCTTCGACGCCGCCTCCACCGTCGTCGACGTCGGCGGCGGCGTGGGCGCCACTGTGGCCGCCGTCGTCTCCCGCCACCCGCACATCCGGGGGATCAACTACGACCTCCCCCACGTCATCTCCGAGGCGCCGCCGTTCCCCGGGGTGGAGCACGTCGGCGGCGACATGTTCGCCTCCGTGCCCCGCGGCGGCGACGCCATCCTGATGAAGTGGATCCTCCACGACTGGAGCGACGAGCACTGCGCGCGGCTGCTCAAGAACTGCTACGACGCGCTGCCGGAGCACGGGAAGGTGGTGGTGGTGGAGTGCGTGCTGCCGGAGAGCTCCGACGCGACGGCGAGGGAGCAGGGGGTGTTCCACGTCGACATGATCATGCTCGCCCACAACCCCGGCGGCAAGGAGAGGTACGAGAGGGAGTTCAGGGAGCTCGCCCGCGCCGCCGGATTCACCGGCTTCAAGGCCACCTACATCTACGCCAACGCCTGGGCCATCGAGTTCACCAAGTAG
Protein Sequence
- >LOC_Os08g06100.1
MGSTAADMAAAADEEACMYALQLASSSILPMTLKNAIELGLLETLQSAAVAGGGGKAALLTPAEVADKLPSKANPAAADMVDRMLRLLASYNVVRCEMEEGADGKLSRRYAAAPVCKWLTPNEDGVSMAALALMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMTAFEYHGTDARFNRVFNEGMKNHSVIITKKLLDLYTGFDAASTVVDVGGGVGATVAAVVSRHPHIRGINYDLPHVISEAPPFPGVEHVGGDMFASVPRGGDAILMKWILHDWSDEHCARLLKNCYDALPEHGKVVVVECVLPESSDATAREQGVFHVDMIMLAHNPGGKERYEREFRELARAAGFTGFKATYIYANAWAIEFTK*