Gene Details:

Functional Descriptions:

  • The expression of OsTOP6A2, OsTOP6A3 and OsTOP6B was differentially regulated by the plant hormones, auxin, cytokinin, and abscisic acid.
  • The transcript abundance of OsTOP6A2, OsTOP6A3 and OsTOP6B was also higher in prepollinated flowers and callus.
  • Similar to Osspo11-1, the pollen mother cells of OsSPO11-2 T1 progeny plants, showed an absence of bivalent formation at metaphase I, aberrant segregation of homologous chromosomes and formation of nonviable tetrads.
  • Similar to Osspo11-1, biallelic mutations in OsSPO11-2 first exon led to complete panicle sterility, and consequently, none of these primary transformants produced seeds.
  • Assessment of the roles of OsSPO11-2 and OsSPO11-4 in rice meiosis using CRISPR/Cas9 mutagenesis.
  • Altogether, this study demonstrates that whereas OsSPO11-1 and OsSPO11-2 are the likely orthologs of Arabidopsis AtSPO11-1 and AtSPO11-2, the additional OsSPO11-4 gene has no major role in wild-type rice meiosis.
  • As expected, we observed very few DMC1 foci (19 on average) in the sterile Osspso11-1 mutant but surprisingly 85 foci in the sterile OsSPO11-2 mutant, indicative of a divergent function between these two SPO11 genes in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os08g06050.1
    AGCGGAAACCTGGGCGAGTTGTCCGTTGGCGCCGCCGCCGGCGAGCTCGCGCACGGCCGCCGCGGCAGTACGGGATGGCGGAGGCGGGAGTGGCGGCCGCCTCGCTCTTCGGCGCCGACCGCCGCCTCTGCTCCGCCGACATCCTCCCTCCCGCCGAGGTGCGGGCGAGGATCGAGGTGGCGGTGCTCAACTTCCTCGCGGCGCTCACCGACCCGGCCGCACCGGCCATCTCCGCCCTCCCGCTGATCAGCCGGGGCGCCGCCAACCGCGGCCTGCGGCGCGCGCTGCTGCGCGACGACGTCTCGTCGGTGTACCTCTCCTACGCCTCCTGCAAGAGATCCCTCACGAGGGCCAACGACGCCAAGGCATTCGTTAGAGTGTGGAAGGTGATGGAGATGTGCTACAAGATTTTGGGGGAGGGGAAGCTGGTCACCCTGAGGGAGCTGTTCTACACGCTCCTCTCTGAATCGCCCACCTACTTCACCTGCCAGCGGCATGTCAACCAGACTGTTCAAGATGTCGTCTCGTTGCTCCGGTGTACGAGGCAGAGCCTGGGGATCATGGCGTCGAGTAGAGGGGCACTGATTGGGCGGCTTGTGGTGCAGGGGCCGGAGGAAGAACATGTTGACTGCTCCATTCTTGGACCTTCTGGGCATGCGATCACTGGAGATCTCAATGTATTGAGCAAATTAATCTTTTCTTCAGATGCGCGGTATATCATTGTAGTGGAGAAGGATGCCATATTTCAGAGGCTGGCTGAAGATCGCATATATAGTCACCTTCCGTGTATCCTGATTACTGCAAAAGGATATCCTGATCTTGCCACAAGGTTTATCTTGCATCGACTGAGCCAGACTTATCCGAACATGCCGATTTTCGCATTAGTAGACTGGAACCCAGCAGGGCTTGCTATACTATGCACTTACAAGTATGGAAGCATTTCAATGGGTTTGGAATCATACAGATATGCTTGCAATGTAAAATGGCTTGGCCTAAGAGGAGATGATCTGCAGCTTATACCTCAGAGCGCATATCAAGAACTGAAGCCACGTGATCTGCAGATTGCCAAAAGCTTGCTGTCATCTAAGTTTCTGCAGGACAAGCACAGAGCTGAGCTGACACTGATGTTGGAGACAGGCAAGCGTGCCGAGATCGAAGCTCTCTACTCACATGGGTTTGATTTCCTGGGGAAATATGTTGCAAGGAAGATTGTACAGGGTGATTACATTTGACAGTGCATTGCTGCCTGCCTCATGGTTTCTCTGGTTTTACTGAGAACTGAAATTTTGAGCTGATTCCATCAGCCCTGGCCCTGTCCAGTTTTCTTCAAAAATGACTTGTATCTTTGAAGAATGTTGCTGTTGTTACTGCACGTCCCCTGCGTGAGTATGCTGTATGCAGAATACTTGAAATTATTACACCATCTGAAGGTGAAGGAGAAATCTGAACCTTGACATACACAAAAGGATACTGCTATACATCCCCAGAATGTCAGTAGTAAACTGCTCAGCTGTGGACTACTGGATGTAAATTGTAGTCTAGCAGTTGCATATATCTCCTCCTTTTTACCTCCTTTATTAAACAAAGATCAAACGGTCTTTAGTGTGTTAACACATGGAATATTGTCGGATTTACTGCAGTTCTAAGGTGCTGTTTGATTCACTTCC
CDS Sequence
  • >LOC_Os08g06050.1
    ATGGCGGAGGCGGGAGTGGCGGCCGCCTCGCTCTTCGGCGCCGACCGCCGCCTCTGCTCCGCCGACATCCTCCCTCCCGCCGAGGTGCGGGCGAGGATCGAGGTGGCGGTGCTCAACTTCCTCGCGGCGCTCACCGACCCGGCCGCACCGGCCATCTCCGCCCTCCCGCTGATCAGCCGGGGCGCCGCCAACCGCGGCCTGCGGCGCGCGCTGCTGCGCGACGACGTCTCGTCGGTGTACCTCTCCTACGCCTCCTGCAAGAGATCCCTCACGAGGGCCAACGACGCCAAGGCATTCGTTAGAGTGTGGAAGGTGATGGAGATGTGCTACAAGATTTTGGGGGAGGGGAAGCTGGTCACCCTGAGGGAGCTGTTCTACACGCTCCTCTCTGAATCGCCCACCTACTTCACCTGCCAGCGGCATGTCAACCAGACTGTTCAAGATGTCGTCTCGTTGCTCCGGTGTACGAGGCAGAGCCTGGGGATCATGGCGTCGAGTAGAGGGGCACTGATTGGGCGGCTTGTGGTGCAGGGGCCGGAGGAAGAACATGTTGACTGCTCCATTCTTGGACCTTCTGGGCATGCGATCACTGGAGATCTCAATGTATTGAGCAAATTAATCTTTTCTTCAGATGCGCGGTATATCATTGTAGTGGAGAAGGATGCCATATTTCAGAGGCTGGCTGAAGATCGCATATATAGTCACCTTCCGTGTATCCTGATTACTGCAAAAGGATATCCTGATCTTGCCACAAGGTTTATCTTGCATCGACTGAGCCAGACTTATCCGAACATGCCGATTTTCGCATTAGTAGACTGGAACCCAGCAGGGCTTGCTATACTATGCACTTACAAGTATGGAAGCATTTCAATGGGTTTGGAATCATACAGATATGCTTGCAATGTAAAATGGCTTGGCCTAAGAGGAGATGATCTGCAGCTTATACCTCAGAGCGCATATCAAGAACTGAAGCCACGTGATCTGCAGATTGCCAAAAGCTTGCTGTCATCTAAGTTTCTGCAGGACAAGCACAGAGCTGAGCTGACACTGATGTTGGAGACAGGCAAGCGTGCCGAGATCGAAGCTCTCTACTCACATGGGTTTGATTTCCTGGGGAAATATGTTGCAAGGAAGATTGTACAGGGTGATTACATTTGA
Protein Sequence
  • >LOC_Os08g06050.1
    MAEAGVAAASLFGADRRLCSADILPPAEVRARIEVAVLNFLAALTDPAAPAISALPLISRGAANRGLRRALLRDDVSSVYLSYASCKRSLTRANDAKAFVRVWKVMEMCYKILGEGKLVTLRELFYTLLSESPTYFTCQRHVNQTVQDVVSLLRCTRQSLGIMASSRGALIGRLVVQGPEEEHVDCSILGPSGHAITGDLNVLSKLIFSSDARYIIVVEKDAIFQRLAEDRIYSHLPCILITAKGYPDLATRFILHRLSQTYPNMPIFALVDWNPAGLAILCTYKYGSISMGLESYRYACNVKWLGLRGDDLQLIPQSAYQELKPRDLQIAKSLLSSKFLQDKHRAELTLMLETGKRAEIEALYSHGFDFLGKYVARKIVQGDYI*