Gene Details:

• MSU gene ID: LOC_Os08g05440
• RAPdb gene ID: Os08g0150150
• Gene Symbol: Pi36
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• To physically map this locus, the Pi36(t)-linked markers were mapped by electronic hybridization to bacterial artificial chromosome (BAC) or P1 artificial chromosome (PAC) clones of Nipponbare, and a contig map was constructed in silico through Pairwise BLAST analysis.
• Genetic and physical mapping of Pi36(t), a novel rice blast resistance gene located on rice chromosome 8.
• The effect of the rice blast resistance gene Pi36 on the expression of disease resistance-related genes.
• Interaction between the coiled-coil domain of rice blast resistance protein Pi36 and methyl-jasmonate (MeJA) was studied by fluorescence and UV-vis spectroscopic techniques.
• Study on the interaction between methyl jasmonate and the coiled-coil domain of rice blast resistance protein Pi36 by spectroscopic methods.
• The Pi36-encoded protein is composed of 1056 amino acids, with a single substitution event (Asp to Ser) at residue 590 associated with the resistant phenotype.
• The induction of the expression of genes from both of these families suggests that both the JA and SA pathways are involved in Pi36-mediated defence.
• The resistance gene Pi36 confers a stable and high level of resistance against Chinese isolates of the rice blast pathogen Magnaporthe oryzae.
• The binding sites of MeJA with the coiled-coil structural domain of rice blast resistance protein Pi36 were found to approach the microenvironment of both Tyr and Trp by the synchronous fluorescence spectrometry.
• The distance r between donor (the coiled-coil domain of rice blast resistance protein Pi36) and acceptor (MeJA) was obtained according to Forster theory of non-radioactive energy transfer.
• The indica rice variety Kasalath carries Pi36, a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8.
• Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063, which allowed the identification of Pi36-3 as the functional gene, with the other two candidates being probable pseudogenes.
• Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita, Pib, Pi9, and Piz-t.
• The in silico map-based cloning of Pi36, a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus.

Function-related keywords:

• blast ,  disease ,  jasmonate ,  disease-resistance ,  resistant ,  sa ,  blast-resistance ,  ja ,  magnaporthe-oryzae

Literature:

• Study on the interaction between methyl jasmonate and the coiled-coil domain of rice blast resistance protein Pi36 by spectroscopic methods .  DOI:  10.1016/j.saa.2011.11.058 ;  PMID:  22196797
• The in silico map-based cloning of Pi36, a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus .  DOI:  10.1534/genetics.107.075465 ;  PMID:  17507669
• Genetic and physical mapping of Pi36(t), a novel rice blast resistance gene located on rice chromosome 8 .  DOI:  10.1007/s00438-005-0032-5 ;  PMID:  16151856
• The effect of the rice blast resistance gene Pi36 on the expression of disease resistance-related genes .  DOI:  10.1007/s11434-010-3264-7

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: