Information report for OsFIE1;Epi-df
Gene Details
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Functional Descriptions
- Here, we identify a gain-of-function epi-allele (Epi-df) of rice (Oryza sativa) FIE1; this allele causes a dwarf stature and various floral defects that are inherited in a dominant fashion.
- OsFIE1 is expressed only in endosperm; the maternal copy is expressed while the paternal copy is not active.
- Imprinted Gene OsFIE1 Modulates Rice Seed Development by Influencing the Nutrient Metabolism and Modifying Genome H3K27me3.
- The RNAi of OsFIE1 and homozygous T-DNA insertion mutant OsFIE1 led to smaller seed, delayed embryo development, smaller aleurone layer cell, and decreased seed set rate.
- OsFIE1 specifically expressed in endosperm, and mRNA of OsFIE1 also enriched in inner seed coat with the corresponding PcG members of OsiEZ1 and OsCLF.
- Meanwhile, the contents of seed storage proteins and Ile, Leu, and Val were decreased, accompanied with the down-regulation of multiple transcription factors, storage protein synthesis and amino acid metabolism related genes in OsFIE1-RNAi lines and OsFIE1.
- The newly evolved N-terminal tail of OsFIE1 was probably not the cause of the adverse effects on vegetative growth.
- The mutant OsFIE1 produced smaller seeds and displayed reduced dormancy, indicating that OsFIE1 predominantly functions in late seed development.
Functional Keywords
- floral , dwarf , endosperm , seed , development , transcription-factor , seed-development , growth , vegetative
Literature and News
- Identification and characterization of an epi-allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice . DOI: 10.1105/tpc.112.102269 ; PMID: 23150632
- Expression, imprinting, and evolution of rice homologs of the polycomb group genes . DOI: 10.1093/mp/ssp036 ; PMID: 19825651
- Imprinted gene OsFIE1 modulates rice seed development by influencing nutrient metabolism and modifying genome H3K27me3 . DOI: 10.1111/tpj.13202 ; PMID: 27133784
- Allelic variation in rice Fertilization Independent Endosperm 1 contributes to grain width under high night temperature stress . DOI: 10.1111/nph.16897 ; PMID: 32858766
- Functional divergence of two duplicated Fertilization Independent Endosperm genes in rice with respect to seed development . DOI: 10.1111/tpj.14911 ; PMID: 33463824
Gene Resources
- UniProt: Q6ZJW8
- EMBL: AK242200, AP003896, AP008214
- AlphaFoldDB: Q6ZJW8
- EnsemblPlants: Os08t0137250-01
- Gramene: Os08t0137250-01
- KEGG: dosa:Os08g0137250, osa:9271694
- Orthologous matrix: VWEMDPS
- InterPro: IPR001680, IPR015943, IPR019775
Homologs
- Brachypodium distachyon: BRADI_3g14520v3
Sequences
cDNA Sequence
- >LOC_Os08g04290.1
TCCACCCCGCCCGCCGCCGCTCCCTATTCCTATTTAATCCCATCTCTCTTCCTCATCACCGCTCTCCTCTCTCCAGGCAAGAGGAAGTACGCAACCTCAAGCCGGACAAGACGTGAGCAAAGATGGGCCCCACTAGTAGGAACCATAAATCATCTCAAAAAGATGTGGCACCGAATGAAGCCAAACCACCCCGATATCCACAGCGCAACCGCTCCATCACTGCCTCTGCCTCTGCCTCTGCCTTTGCCTCTCCCGCTGTTGCCAACTCCAGAGTTGCCAAGGAAAGGCCATCTTCATCAACTGCTGGTGAAGGTGAACCACAGGAAACGGTGCTAAAGCTTCCAAGCATCCCAACACTTCCTGCACGGATGGCAAAGTTGGTGCCATTAGAGGGGTTGGGATGCGAGGCAGCGGTGGGATCGCTAACACCGAGTCGGGAGCGAGAATACAAGGTGACCAACAAGCACACTGAGGGAAGGCGCCCTGTCTACGCCATTGTCTTCAATTTCCTCGATGTTCGCTACTACGACATCTTCGCCACCGCCTGTGGCCCTCGTCTTTCAACCTACCGCTGCCTCATGAATGGCAAATTTGCTCTTCTGCAAAGCTATCTTGATGACGATATGAATGAGTCATTCTTCACTGTGAGCTGGGCTTGCGACATTGATGGCAATCCATTGTTAGTAGCTGCAGGAAGCACTGGAATCATTCGAGTCATCAACTGTGCCACTGAGAAGATATATAAGAGTCTTGTTGGCCATGGTGGTTCAGTAAACGAAATAAAGTCTCAACCATCGAATCCTTCACTCATCATTTCTGCAAGCAAGGATGAATCTATTAAGCTGTGGAATGTGCAGACAGGGATCTTAATTTTGGTTTTTGGTGGAGTAGGAGGTCACCGACACGAAGTACTTGGTGTTGACTTCCACACATCTGATATCTACCGCTTTTTAAGTTGTGGAATGGACAACACTGTGAGAATCTGGTCAATGAAAGAATTCTGGGAATATGTTGAGAAATCCTATTCATGGACTGATGCTACATCAAAATTTCCAACAAAATTTGTCCAATTTCCGGTCCTGTGTGCTGAAATACATTCTAACTATGTAGACTGTACTAAATGGCTTGGGGACTTTGTCCTGTCAAAGAGTGTTGAAAATGAAATCTTGCTGTGGGAATCGATCACAAAAGAAGAAAACCCTGGCGAGGGTCACATTGATGTTCTTCAGAAGTACCCTGTGCCAGAATGTAACATCTGGTTCATGAAATTCTCATGTGATTTTCACCACAATCAGTTGGCAATAGGAAACCGTGATGGTAAAGTCTATGTCTGGAAAGTACAGACCAGCCCTCCTGTTCTAATTGCTCGGCTCAATAATCCACAAGTGAAATCAGCCATAAGGCAGACTGCAGTGTCCTTTGATGGAAGCACAATCCTTGCCTGCACAGAGGATGGCAACATATGGCGTTGGGATGAAGTGGATCACCCAACCGCCCCAGTCCCAAGCAAGAAACAAAAGTGAAGCAAGGATTATATTAATTGAAATTAATAAGGCTGAATTGGCGATTTGTTCTTGGTATACTTTGTTATACTACTTTGCCTTAGCGTTCATCAAAACTGCCATTTGATTTTCCAACATTCAGAAAACTCTATAGCTGGATGTAAGATTTATTATCGCAAGATATTCAGGATGTTGGTCGGTGACTTGGTTTTATCAATAATTTGCTGGGAATATAGTACTTAATCT
CDS Sequence
- >LOC_Os08g04290.1
ATGGGCCCCACTAGTAGGAACCATAAATCATCTCAAAAAGATGTGGCACCGAATGAAGCCAAACCACCCCGATATCCACAGCGCAACCGCTCCATCACTGCCTCTGCCTCTGCCTCTGCCTTTGCCTCTCCCGCTGTTGCCAACTCCAGAGTTGCCAAGGAAAGGCCATCTTCATCAACTGCTGGTGAAGGTGAACCACAGGAAACGGTGCTAAAGCTTCCAAGCATCCCAACACTTCCTGCACGGATGGCAAAGTTGGTGCCATTAGAGGGGTTGGGATGCGAGGCAGCGGTGGGATCGCTAACACCGAGTCGGGAGCGAGAATACAAGGTGACCAACAAGCACACTGAGGGAAGGCGCCCTGTCTACGCCATTGTCTTCAATTTCCTCGATGTTCGCTACTACGACATCTTCGCCACCGCCTGTGGCCCTCGTCTTTCAACCTACCGCTGCCTCATGAATGGCAAATTTGCTCTTCTGCAAAGCTATCTTGATGACGATATGAATGAGTCATTCTTCACTGTGAGCTGGGCTTGCGACATTGATGGCAATCCATTGTTAGTAGCTGCAGGAAGCACTGGAATCATTCGAGTCATCAACTGTGCCACTGAGAAGATATATAAGAGTCTTGTTGGCCATGGTGGTTCAGTAAACGAAATAAAGTCTCAACCATCGAATCCTTCACTCATCATTTCTGCAAGCAAGGATGAATCTATTAAGCTGTGGAATGTGCAGACAGGGATCTTAATTTTGGTTTTTGGTGGAGTAGGAGGTCACCGACACGAAGTACTTGGTGTTGACTTCCACACATCTGATATCTACCGCTTTTTAAGTTGTGGAATGGACAACACTGTGAGAATCTGGTCAATGAAAGAATTCTGGGAATATGTTGAGAAATCCTATTCATGGACTGATGCTACATCAAAATTTCCAACAAAATTTGTCCAATTTCCGGTCCTGTGTGCTGAAATACATTCTAACTATGTAGACTGTACTAAATGGCTTGGGGACTTTGTCCTGTCAAAGAGTGTTGAAAATGAAATCTTGCTGTGGGAATCGATCACAAAAGAAGAAAACCCTGGCGAGGGTCACATTGATGTTCTTCAGAAGTACCCTGTGCCAGAATGTAACATCTGGTTCATGAAATTCTCATGTGATTTTCACCACAATCAGTTGGCAATAGGAAACCGTGATGGTAAAGTCTATGTCTGGAAAGTACAGACCAGCCCTCCTGTTCTAATTGCTCGGCTCAATAATCCACAAGTGAAATCAGCCATAAGGCAGACTGCAGTGTCCTTTGATGGAAGCACAATCCTTGCCTGCACAGAGGATGGCAACATATGGCGTTGGGATGAAGTGGATCACCCAACCGCCCCAGTCCCAAGCAAGAAACAAAAGTGA
Protein Sequence
- >LOC_Os08g04290.1
MGPTSRNHKSSQKDVAPNEAKPPRYPQRNRSITASASASAFASPAVANSRVAKERPSSSTAGEGEPQETVLKLPSIPTLPARMAKLVPLEGLGCEAAVGSLTPSREREYKVTNKHTEGRRPVYAIVFNFLDVRYYDIFATACGPRLSTYRCLMNGKFALLQSYLDDDMNESFFTVSWACDIDGNPLLVAAGSTGIIRVINCATEKIYKSLVGHGGSVNEIKSQPSNPSLIISASKDESIKLWNVQTGILILVFGGVGGHRHEVLGVDFHTSDIYRFLSCGMDNTVRIWSMKEFWEYVEKSYSWTDATSKFPTKFVQFPVLCAEIHSNYVDCTKWLGDFVLSKSVENEILLWESITKEENPGEGHIDVLQKYPVPECNIWFMKFSCDFHHNQLAIGNRDGKVYVWKVQTSPPVLIARLNNPQVKSAIRQTAVSFDGSTILACTEDGNIWRWDEVDHPTAPVPSKKQK*