Gene Details:

Functional Descriptions:

  • Here, we identify a gain-of-function epi-allele (Epi-df) of rice (Oryza sativa) FIE1; this allele causes a dwarf stature and various floral defects that are inherited in a dominant fashion.
  • OsFIE1 is expressed only in endosperm; the maternal copy is expressed while the paternal copy is not active.
  • Imprinted Gene OsFIE1 Modulates Rice Seed Development by Influencing the Nutrient Metabolism and Modifying Genome H3K27me3.
  • The RNAi of OsFIE1 and homozygous T-DNA insertion mutant OsFIE1 led to smaller seed, delayed embryo development, smaller aleurone layer cell, and decreased seed set rate.
  • OsFIE1 specifically expressed in endosperm, and mRNA of OsFIE1 also enriched in inner seed coat with the corresponding PcG members of OsiEZ1 and OsCLF.
  • Meanwhile, the contents of seed storage proteins and Ile, Leu, and Val were decreased, accompanied with the down-regulation of multiple transcription factors, storage protein synthesis and amino acid metabolism related genes in OsFIE1-RNAi lines and OsFIE1.
  • The newly evolved N-terminal tail of OsFIE1 was probably not the cause of the adverse effects on vegetative growth.
  • The mutant OsFIE1 produced smaller seeds and displayed reduced dormancy, indicating that OsFIE1 predominantly functions in late seed development.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os08g04290.1
    TCCACCCCGCCCGCCGCCGCTCCCTATTCCTATTTAATCCCATCTCTCTTCCTCATCACCGCTCTCCTCTCTCCAGGCAAGAGGAAGTACGCAACCTCAAGCCGGACAAGACGTGAGCAAAGATGGGCCCCACTAGTAGGAACCATAAATCATCTCAAAAAGATGTGGCACCGAATGAAGCCAAACCACCCCGATATCCACAGCGCAACCGCTCCATCACTGCCTCTGCCTCTGCCTCTGCCTTTGCCTCTCCCGCTGTTGCCAACTCCAGAGTTGCCAAGGAAAGGCCATCTTCATCAACTGCTGGTGAAGGTGAACCACAGGAAACGGTGCTAAAGCTTCCAAGCATCCCAACACTTCCTGCACGGATGGCAAAGTTGGTGCCATTAGAGGGGTTGGGATGCGAGGCAGCGGTGGGATCGCTAACACCGAGTCGGGAGCGAGAATACAAGGTGACCAACAAGCACACTGAGGGAAGGCGCCCTGTCTACGCCATTGTCTTCAATTTCCTCGATGTTCGCTACTACGACATCTTCGCCACCGCCTGTGGCCCTCGTCTTTCAACCTACCGCTGCCTCATGAATGGCAAATTTGCTCTTCTGCAAAGCTATCTTGATGACGATATGAATGAGTCATTCTTCACTGTGAGCTGGGCTTGCGACATTGATGGCAATCCATTGTTAGTAGCTGCAGGAAGCACTGGAATCATTCGAGTCATCAACTGTGCCACTGAGAAGATATATAAGAGTCTTGTTGGCCATGGTGGTTCAGTAAACGAAATAAAGTCTCAACCATCGAATCCTTCACTCATCATTTCTGCAAGCAAGGATGAATCTATTAAGCTGTGGAATGTGCAGACAGGGATCTTAATTTTGGTTTTTGGTGGAGTAGGAGGTCACCGACACGAAGTACTTGGTGTTGACTTCCACACATCTGATATCTACCGCTTTTTAAGTTGTGGAATGGACAACACTGTGAGAATCTGGTCAATGAAAGAATTCTGGGAATATGTTGAGAAATCCTATTCATGGACTGATGCTACATCAAAATTTCCAACAAAATTTGTCCAATTTCCGGTCCTGTGTGCTGAAATACATTCTAACTATGTAGACTGTACTAAATGGCTTGGGGACTTTGTCCTGTCAAAGAGTGTTGAAAATGAAATCTTGCTGTGGGAATCGATCACAAAAGAAGAAAACCCTGGCGAGGGTCACATTGATGTTCTTCAGAAGTACCCTGTGCCAGAATGTAACATCTGGTTCATGAAATTCTCATGTGATTTTCACCACAATCAGTTGGCAATAGGAAACCGTGATGGTAAAGTCTATGTCTGGAAAGTACAGACCAGCCCTCCTGTTCTAATTGCTCGGCTCAATAATCCACAAGTGAAATCAGCCATAAGGCAGACTGCAGTGTCCTTTGATGGAAGCACAATCCTTGCCTGCACAGAGGATGGCAACATATGGCGTTGGGATGAAGTGGATCACCCAACCGCCCCAGTCCCAAGCAAGAAACAAAAGTGAAGCAAGGATTATATTAATTGAAATTAATAAGGCTGAATTGGCGATTTGTTCTTGGTATACTTTGTTATACTACTTTGCCTTAGCGTTCATCAAAACTGCCATTTGATTTTCCAACATTCAGAAAACTCTATAGCTGGATGTAAGATTTATTATCGCAAGATATTCAGGATGTTGGTCGGTGACTTGGTTTTATCAATAATTTGCTGGGAATATAGTACTTAATCT
CDS Sequence
  • >LOC_Os08g04290.1
    ATGGGCCCCACTAGTAGGAACCATAAATCATCTCAAAAAGATGTGGCACCGAATGAAGCCAAACCACCCCGATATCCACAGCGCAACCGCTCCATCACTGCCTCTGCCTCTGCCTCTGCCTTTGCCTCTCCCGCTGTTGCCAACTCCAGAGTTGCCAAGGAAAGGCCATCTTCATCAACTGCTGGTGAAGGTGAACCACAGGAAACGGTGCTAAAGCTTCCAAGCATCCCAACACTTCCTGCACGGATGGCAAAGTTGGTGCCATTAGAGGGGTTGGGATGCGAGGCAGCGGTGGGATCGCTAACACCGAGTCGGGAGCGAGAATACAAGGTGACCAACAAGCACACTGAGGGAAGGCGCCCTGTCTACGCCATTGTCTTCAATTTCCTCGATGTTCGCTACTACGACATCTTCGCCACCGCCTGTGGCCCTCGTCTTTCAACCTACCGCTGCCTCATGAATGGCAAATTTGCTCTTCTGCAAAGCTATCTTGATGACGATATGAATGAGTCATTCTTCACTGTGAGCTGGGCTTGCGACATTGATGGCAATCCATTGTTAGTAGCTGCAGGAAGCACTGGAATCATTCGAGTCATCAACTGTGCCACTGAGAAGATATATAAGAGTCTTGTTGGCCATGGTGGTTCAGTAAACGAAATAAAGTCTCAACCATCGAATCCTTCACTCATCATTTCTGCAAGCAAGGATGAATCTATTAAGCTGTGGAATGTGCAGACAGGGATCTTAATTTTGGTTTTTGGTGGAGTAGGAGGTCACCGACACGAAGTACTTGGTGTTGACTTCCACACATCTGATATCTACCGCTTTTTAAGTTGTGGAATGGACAACACTGTGAGAATCTGGTCAATGAAAGAATTCTGGGAATATGTTGAGAAATCCTATTCATGGACTGATGCTACATCAAAATTTCCAACAAAATTTGTCCAATTTCCGGTCCTGTGTGCTGAAATACATTCTAACTATGTAGACTGTACTAAATGGCTTGGGGACTTTGTCCTGTCAAAGAGTGTTGAAAATGAAATCTTGCTGTGGGAATCGATCACAAAAGAAGAAAACCCTGGCGAGGGTCACATTGATGTTCTTCAGAAGTACCCTGTGCCAGAATGTAACATCTGGTTCATGAAATTCTCATGTGATTTTCACCACAATCAGTTGGCAATAGGAAACCGTGATGGTAAAGTCTATGTCTGGAAAGTACAGACCAGCCCTCCTGTTCTAATTGCTCGGCTCAATAATCCACAAGTGAAATCAGCCATAAGGCAGACTGCAGTGTCCTTTGATGGAAGCACAATCCTTGCCTGCACAGAGGATGGCAACATATGGCGTTGGGATGAAGTGGATCACCCAACCGCCCCAGTCCCAAGCAAGAAACAAAAGTGA
Protein Sequence
  • >LOC_Os08g04290.1
    MGPTSRNHKSSQKDVAPNEAKPPRYPQRNRSITASASASAFASPAVANSRVAKERPSSSTAGEGEPQETVLKLPSIPTLPARMAKLVPLEGLGCEAAVGSLTPSREREYKVTNKHTEGRRPVYAIVFNFLDVRYYDIFATACGPRLSTYRCLMNGKFALLQSYLDDDMNESFFTVSWACDIDGNPLLVAAGSTGIIRVINCATEKIYKSLVGHGGSVNEIKSQPSNPSLIISASKDESIKLWNVQTGILILVFGGVGGHRHEVLGVDFHTSDIYRFLSCGMDNTVRIWSMKEFWEYVEKSYSWTDATSKFPTKFVQFPVLCAEIHSNYVDCTKWLGDFVLSKSVENEILLWESITKEENPGEGHIDVLQKYPVPECNIWFMKFSCDFHHNQLAIGNRDGKVYVWKVQTSPPVLIARLNNPQVKSAIRQTAVSFDGSTILACTEDGNIWRWDEVDHPTAPVPSKKQK*