Information report for OsAPX2;APXb
Gene Details
|
|
Functional Descriptions
- Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility.
- Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.
- Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.
- In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses.
- OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses.
- Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.
- OsAPX2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance.
- Loss of OsAPX2 altered rice architecture including shoot height and leaf inclination, resulting in shoot dwarfing, leaf dispersion and fertility decline.
- Additionally, the OsAPX2 conformational changes could be regulated by salt and heat stresses and these stimulated OsAPX2 dissociation and association, respectively.
Functional Keywords
- leaf , growth , sterility , salt , cold-stress , seedling , drought , seed , abiotic-stress , dwarf , shoot , architecture , height , fertility
Literature and News
- Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings . DOI: 10.1016/j.jplph.2011.11.012 ; PMID: 22196946
- Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling . DOI: 10.1016/j.gene.2003.08.017 ; PMID: 14644501
- Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses . DOI: 10.1371/journal.pone.0057472 ; PMID: 23468992
- Genetic transformation and analysis of rice OsAPx2 gene in Medicago sativa . DOI: 10.1371/journal.pone.0041233 ; PMID: 22848448
- Purification and characterization of two ascorbate peroxidases of rice (Oryza sativa L.) expressed in Escherichia coli . DOI: 10.1007/s10529-004-6587-0 ; PMID: 15685422
- Cytosolic APX2 is a pleiotropic protein involved in H2O2 homeostasis, chloroplast protection, plant architecture and fertility maintenance . DOI: 10.1007/s00299-018-2272-y ; PMID: 29549445
- Functional switching of ascorbate peroxidase 2 of rice (OsAPX2) between peroxidase and molecular chaperone . DOI: 10.1038/s41598-018-27459-1 ; PMID: 29907832
Gene Resources
- NCBI ID: AB053297
Sequences
cDNA Sequence
- >LOC_Os07g49400.3
GCACCCTCGCGCCTCGCCTTTATATTACTAGTAAGAAGATCTCATCCCTCCTTGGTGAGGTGAGGTGAGTTGAGTTGGGGATTGATTGATTGATTCGGATTGGGAAGAAGAAGAAGCAGGGGAGCATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAAAGATGCATCCTGTCTGTTGATAAACAGCCAGCCACAGCTTGTTTCCTTGTATGCTAAAGAATGAAAACCAAAATCTCCTCTCGATCAACAGCGAGCTTGTGAGTGGCGAGAAGGAAGGCCTTCTTCAGCTGCCAAGTGACAAAGCCCTCATGGCTGACCCAGCCTTCCGTCCACTGGTGGAGAAATATGCTGCGGATGAGGACGCCTTCTTTGCGGATTACGCCGAGGCCCACCTCAAGCTCTCTGAACTGGGATTTGCTGAGGAATAAGAAGCCTTTAGAGAGCGGGATATCCGCAAAAGATTAATGCCGATTTGTATTTTGCGCCTTAGAGTCAGTACGATCAAGACTGTCGTGGCGGTTGTAATAAAAATTAGTGTGCTTTGGGCCATCTTTTTATGTGATTCCAATTGTCTTTCTCTTCATTCTTGCTTTGATGCTCTTTGTCTGGACCTCTAGACCGCCGTATTGTACTGTGGAGTTTCAAAGTTACCAAGCTATTTGCTGTCAAGATAACTATGGATTGAATTCCCCTTGATGGATGAACCAACTGTTGTTGTTTGCCCGTTCTTCAGCTTTCGTTTGTGCGGCCATCGATCGCCATGCGTTGCTTAAACCCATTTCTAGCTCCCCTACCCTGCTGCATCCGCCCTCTTCTGCGCGATCGTTGGATTGCGAGTGGTTGGCTGGTTGCACGACTTGTGGAGACCGAAACAAATAATTTTTGGTCAAATTGATCGGTGGTACTGTCGGAGCATCTATTTTTTCTTTAGCTTAGATCGTATAATTGTAGGATTGGGATTTGTATATTAATATATACAGGTCGATTAAAACAATGCAACTATTCGTGATGTCATGTGACCT - >LOC_Os07g49400.2
GCACCCTCGCGCCTCGCCTTTATATTACTAGTAAGAAGATCTCATCCCTCCTTGGTGAGGTGAGGTGAGTTGAGTTGGGGATTGATTGATTGATTCGGATTGGGAAGAAGAAGAAGCAGGGGAGCATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAAAGATGCATCCTGTCTGTTGATAAACAGCCAGCCACAGCTTGTTTCCTTGTATGCTAAAGAATGAAAACCAAAATCTCCTCTCGATCAACAGCGAGCTTGTGAGTGGCGAGAAGGAAGGCCTTCTTCAGCTGCCAAGTGACAAAGCCCTCATGGCTGACCCAGCCTTCCGTCCACTGGTGGAGAAATATGCTGCGGATGAGGACGCCTTCTTTGCGGATTACGCCGAGGCCCACCTCAAGCTCTCTGAACTGGGATTTGCTGAGGAATAAGAAGCCTTTAGAGAGCGGGATATCCGCAAAAGATTAATGCCGATTTGTATTTTGCGCCTTAGAGTCAGTACGATCAAGACTGTCGTGGCGGTTGTAATAAAAATTAGTGTGCTTTGGGCCATCTTTTTATGTGATTCCAATTGTCTTTCTCTTCATTCTTGCTTTGATGCTCTTTGTCTGGACCTCTAGACCGCCGTATTGTACTGTGGAGTTTCAAAGTTACCAAGCTATTTGCTGTCAAGATAACTATGGATTGAATTCCCCTTGATGGATGAACCAACTGTTGTTGTTTGCCCGTTCTTCAGCTTTCGTTTGTGCGGCCATCGATCGCCATGCGTTGCTTAAACCCATTTCTAGCTCCCCTACCCTGCTGCATCCGCCCTCTTCTGCGCGATCGTTGGATTGCGAGTGGTTGGCTGGTTGCACGACTTGTGGAGACCGAAACAAATAATTTTTGGTCAAATTGATCGGTGGTACTGTCGGAGCATCTATTTTTTCTTTAGCTTAGATCGTATAATTGTAGGATTGGGATTTGTATATTAATATATACAGGTCGATTAAAACAATGCAACTATTCGTGATGTCATGTGACCT - >LOC_Os07g49400.1
GCACCCTCGCGCCTCGCCTTTATATTACTAGTAAGAAGATCTCATCCCTCCTTGGTGAGGTGAGGTGAGTTGAGTTGGGGATTGATTGATTGATTCGGATTGGGAAGAAGAAGAAGCAGGGGAGCATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAAAGATGCATCCTGTCTGTTGATAAACAGCCAGCCACAGCTTGTTTCCTTGTATGCTAAAGAATGAAAACCAAAATCTCCTCTCGATCAACAGCGAGCTTGTGAGTGGCGAGAAGGAAGGCCTTCTTCAGCTGCCAAGTGACAAAGCCCTCATGGCTGACCCAGCCTTCCGTCCACTGGTGGAGAAATATGCTGCGGATGAGGACGCCTTCTTTGCGGATTACGCCGAGGCCCACCTCAAGCTCTCTGAACTGGGATTTGCTGAGGAATAAGAAGCCTTTAGAGAGCGGGATATCCGCAAAAGATTAATGCCGATTTGTATTTTGCGCCTTAGAGTCAGTACGATCAAGACTGTCGTGGCGGTTGTAATAAAAATTAGTGTGCTTTGGGCCATCTTTTTATGTGATTCCAATTGTCTTTCTCTTCATTCTTGCTTTGATGCTCTTTGTCTGGACCTCTAGACCGCCGTATTGTACTGTGGAGTTTCAAAGTTACCAAGCTATTTGCTGTCAAGATAACTATGGATTGAATTCCCCTTGATGGATGAACCAACTGTTGTTGTTTGCCCGTTCTTCAGCTTTCGTTTGTGCGGCCATCGATCGCCATGCGTTGCTTAAACCCATTTCTAGCTCCCCTACCCTGCTGCATCCGCCCTCTTCTGCGCGATCGTTGGATTGCGAGTGGTTGGCTGGTTGCACGACTTGTGGAGACCGAAACAAATAATTTTTGGTCAAATTGATCGGTGGTACTGTCGGAGCATCTATTTTTTCTTTAGCTTAGATCGTATAATTGTAGGATTGGGATTTGTATATTAATATATACAGGTCGATTAAAACAATGCAACTATTCGTGATGTCATGTGACCT - >LOC_Os07g49400.4
GCACCCTCGCGCCTCGCCTTTATATTACTAGTAAGAAGATCTCATCCCTCCTTGGTGAGGTGAGGTGAGTTGAGTTGGGGATTGATTGATTGATTCGGATTGGGAAGAAGAAGAAGCAGGGGAGCATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAAAGATGCATCCTGTCTGTTGATAAACAGCCAGCCACAGCTTGTTTCCTTGTATGCTAAAGAATGAAAACCAAAATCTCCTCTCGATCAACAGCGAGCTTGTGAGTGGCGAGAAGGAAGGCCTTCTTCAGCTGCCAAGTGACAAAGCCCTCATGGCTGACCCAGCCTTCCGTCCACTGGTGGAGAAATATGCTGCGGATGAGGACGCCTTCTTTGCGGATTACGCCGAGGCCCACCTCAAGCTCTCTGAACTGGGATTTGCTGAGGAATAAGAAGCCTTTAGAGAGCGGGATATCCGCAAAAGATTAATGCCGATTTGTATTTTGCGCCTTAGAGTCAGTACGATCAAGACTGTCGTGGCGGTTGTAATAAAAATTAGTGTGCTTTGGGCCATCTTTTTATGTGATTCCAATTGTCTTTCTCTTCATTCTTGCTTTGATGCTCTTTGTCTGGACCTCTAGACCGCCGTATTGTACTGTGGAGTTTCAAAGTTACCAAGCTATTTGCTGTCAAGATAACTATGGATTGAATTCCCCTTGATGGATGAACCAACTGTTGTTGTTTGCCCGTTCTTCAGCTTTCGTTTGTGCGGCCATCGATCGCCATGCGTTGCTTAAACCCATTTCTAGCTCCCCTACCCTGCTGCATCCGCCCTCTTCTGCGCGATCGTTGGATTGCGAGTGGTTGGCTGGTTGCACGACTTGTGGAGACCGAAACAAATAATTTTTGGTCAAATTGATCGGTGGTACTGTCGGAGCATCTATTTTTTCTTTAGCTTAGATCGTATAATTGTAGGATTGGGATTTGTATATTAATATATACAGGTCGATTAAAACAATGCAACTATTCGTGATGTCATGTGACCT
CDS Sequence
- >LOC_Os07g49400.3
ATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAA - >LOC_Os07g49400.2
ATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAA - >LOC_Os07g49400.1
ATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAA - >LOC_Os07g49400.4
ATGGGCAGCAAGTCGTACCCGACGGTGAGCGATGAGTACCTGGCGGCCGTGGGCAAGGCGAAGCGCAAGCTCCGCGGCCTCATCGCCGAGAAGAACTGCGCCCCACTCATGCTCCGCCTCGCGTGGCACTCTGCTGGCACCTTCGATGTGTCGTCGAGGACCGGCGGGCCCTTCGGCACCATGAAGAACCCCGGCGAGCAGTCCCACGCCGCCAACGCCGGCCTCGACATCGCCGTCAGGCTTCTCGACCCCATCAAGGACCAACTTCCCATCCTCTCCTACGCCGACTTCTACCAGCTTGCTGGCGTTGTGGCCGTCGAGGTCACCGGCGGACCTGAGGTCCCCTTCCATCCGGGCAGGCAGGACAAGCCTGAGCCTCCTCCTGAAGGCCGTCTTCCTGATGCCACACAAGGTTCTGACCACCTAAGGCAGGTCTTTTCTGCGCAGATGGGTTTGAGTGACAAGGACATAGTTGCTCTTTCTGGTGGTCACACCCTGGGAAGATGCCACAAGGAGAGATCTGGCTTTGAGGGAGCCTGGACGTCCAACCCTTTGATCTTTGACAACTCTTACTTCACGTGCGTGATCTCTGATTCTTTTTAA
Protein Sequence
- >LOC_Os07g49400.3
MGSKSYPTVSDEYLAAVGKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKNPGEQSHAANAGLDIAVRLLDPIKDQLPILSYADFYQLAGVVAVEVTGGPEVPFHPGRQDKPEPPPEGRLPDATQGSDHLRQVFSAQMGLSDKDIVALSGGHTLGRCHKERSGFEGAWTSNPLIFDNSYFTCVISDSF* - >LOC_Os07g49400.2
MGSKSYPTVSDEYLAAVGKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKNPGEQSHAANAGLDIAVRLLDPIKDQLPILSYADFYQLAGVVAVEVTGGPEVPFHPGRQDKPEPPPEGRLPDATQGSDHLRQVFSAQMGLSDKDIVALSGGHTLGRCHKERSGFEGAWTSNPLIFDNSYFTCVISDSF* - >LOC_Os07g49400.1
MGSKSYPTVSDEYLAAVGKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKNPGEQSHAANAGLDIAVRLLDPIKDQLPILSYADFYQLAGVVAVEVTGGPEVPFHPGRQDKPEPPPEGRLPDATQGSDHLRQVFSAQMGLSDKDIVALSGGHTLGRCHKERSGFEGAWTSNPLIFDNSYFTCVISDSF* - >LOC_Os07g49400.4
MGSKSYPTVSDEYLAAVGKAKRKLRGLIAEKNCAPLMLRLAWHSAGTFDVSSRTGGPFGTMKNPGEQSHAANAGLDIAVRLLDPIKDQLPILSYADFYQLAGVVAVEVTGGPEVPFHPGRQDKPEPPPEGRLPDATQGSDHLRQVFSAQMGLSDKDIVALSGGHTLGRCHKERSGFEGAWTSNPLIFDNSYFTCVISDSF*