Gene Details:

• MSU gene ID: LOC_Os07g49310
• RAPdb gene ID: Os07g0693800
• Gene Symbol: OsFAD8
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• RNA in situ hybridization showed that the accumulation of OsFAD8 mRNA was abundant in leaves but hardly detectable in roots.
• Fatty acid analysis of homozygous OsFAD8 T-DNA knockout mutant and wild type plants have exhibited changes in fatty acid composition after cold stress, thus confirming that OsFAD8 gene codes for omega-3 fatty acid desaturase activity at low temperature.
• Photosynthetic efficiency and recovery of OsFAD8 knockout mutants are significantly reduced after cold stress as compared to those of wild type plants.
• The OsFAD8 transcript level in leaves was much higher at 15 degrees C than at normal temperature (25 degrees C).
• These data suggest that OsFAD8 encodes a temperature-dependent chloroplast omega-3 fatty acid desaturase.
• Expression of two rice plastidial omega-3 desaturase genes, OsFAD7 and OsFAD8 under different temperatures has been investigated.
• Transcript levels of OsFAD7 increased at high temperature; while those of OsFAD8 increased at low temperatures.
• Characterization of OsFAD8 suggests that it has a functional role in maintaining levels of trienoic fatty acids and stress tolerance at low temperatures.
• Effects of low temperature stress on rice (Oryza sativa L.) plastid omega-3 desaturase gene, OsFAD8 and its functional analysis using T-DNA mutants.
• A cDNA, designated OsFAD8, encoding a chloroplast omega-3 fatty acid desaturase responsible for trienoic fatty acid formation, was isolated from the leaves of Oryza sativa L.
• At room temperature (28 C), OsFAD8 plants exhibited significant alterations in fatty acid (FA) unsaturation for all four investigated plastidic lipid classes.
• Taken together, our findings suggest that FA unsaturation by OsFAD8 is crucial for the acclimation of higher plants to low-temperature stress.

Function-related keywords:

• root ,  cold-stress ,  temperature ,  chloroplast ,  stress

Literature:

• Characterization of a rice (Oryza sativa L.) gene encoding a temperature-dependent chloroplast omega-3 fatty acid desaturase .  DOI:  10.1016/j.bbrc.2005.12.126 ;  PMID:  16406238
• Effects of low temperature stress on rice ( Oryza sativa L.) plastid ω-3 desaturase gene, OsFAD8 and its functional analysis using T-DNA mutants .  DOI:  10.1007/s11240-009-9541-y
• Identification and evaluation of ω-3 fatty acid desaturase genes for hyperfortifying α-linolenic acid in transgenic rice seed .  DOI:  10.1093/jxb/ers051 ;  PMID:  22378946
• Rice mutants deficient in ω-3 fatty acid desaturase (FAD8) fail to acclimate to cold temperatures .  DOI:  10.1016/j.plaphy.2016.11.001 ;  PMID:  27835850

Related News:

Gene Resources:

• NCBI ID: AB232383  , XM_506593
• UniProt accessions:

Sequences: