Gene Details:

Functional Descriptions:

  • In contrast, the OsNAS3 transcript was present in leaves but was very low in roots of Fe-sufficient plants.
  • Further, OsNAS3 expression was induced in roots but was suppressed in leaves in response to Fe deficiency.
  • OsNAS3 expression was restricted to the pericycle and companion cells of the roots, and in companion cells of leaves irrespective of Fe status.
  • Shoots and roots of the OsNAS3 activation-tagged plants (OsNAS3-D1) accumulated more Fe and Zn.
  • Seeds from our OsNAS3-D1 plants grown on a paddy field contained elevated amounts of Fe (2.
  • 6-fold in OsNAS3-D1 seeds.
  • Analysis by size exclusion chromatography coupled with inductively coupled plasma mass spectroscopy showed that WT and OsNAS3-D1 seeds contained equal amounts of Fe bound to IP6, whereas OsNAS3-D1 had 7-fold more Fe bound to a low molecular mass, which was likely NA.
  • Differential expression of selected genes (encoding 7 transport proteins, the OsNAS3 enzyme and the OsNAC5 transcription factor) was confirmed by quantitative RT-PCR.
  • Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are expressed in cells involved in long-distance transport of iron and differentially regulated by iron.
  • Our research suggests that simultaneous activation of OsNAS2 and OsNAS3 can enhance Fe and Zn accumulation in rice grains while also increasing plant tolerance to growing situations with metal deficiency and excess metal availability.
  • Concomitant Activation of OsNAS2 and OsNAS3 Contributes to the Enhanced Accumulation of Iron and Zinc in Rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os07g48980.1
    GCACTCCAATCCAAAAGTATAATCAAGCAAAGCAGCTCGATCGAGTGTTCGACTGATCACCAGTTGGAGCTAATCGATCGAGAGAGAGAGTATGACGGTGGAAGTGGAGGCGGTGACCATGGCGAAGGAGGAGCAGCCGGAGGAGGAGGAGGTGATCGAGAAGTTGGTTGAGAAGATCACCGGGCTGGCGGCGGCCATCGGCAAGCTGCCGTCGCTGAGCCCGTCGCCGGAGGTGAACGCGCTGTTCACGGAGCTGGTGATGACCTGCATCCCGCCCAGCAGCGTGGACGTGGAGCAGCTGGGGGCGGAGGCGCAGGACATGCGCGGCCGCCTCATCCGCCTCTGCGCCGACGCCGAGGGCCACCTCGAGGCGCACTACTCCGACGTCCTCGCCGCCCACGACAACCCGCTCGACCACCTCGCCCTCTTCCCCTACTTCAACAACTACATCCAGCTCGCCCAGCTCGAGTACGCCCTCCTCGCCCGCCACCTCCCCGCCGCCCCGCCGCCCTCCCGCCTCGCCTTCCTCGGCTCCGGCCCGCTCCCGCTCAGCTCCCTCGTCCTCGCCGCCCGCCACCTCCCCGCCGCCTCCTTCCACAACTACGACATCTGCGCCGACGCCAACCGCCGCGCCAGCCGCCTCGTCCGCGCCGACCGCGACCTGTCCGCGCGCATGGCCTTCCACACCTCCGACGTCGCCCACGTCACCACCGACCTCGCCGCCTACGACGTCGTGTTCCTGGCGGCGCTGGTGGGTATGGCCGCCGAGGAGAAGGCGCGCATGGTGGAGCACCTCGGGAAGCACATGGCGCCCGGCGCCGCCCTGGTGGTGCGGAGCGCGCACGGCGCCCGGGGATTCCTGTACCCCGTGGTGGACCCGGAGGAGATCCGCCGCGGCGGCTTCGACGTGCTCGCCGTGCACCACCCGGAGGGCGAGGTGATCAACTCCGTCATCATCGCGCGCAAGCCGCCCGTGGCGGCGCCGGCGTTGGAGGGAGGAGACGCGCACGCGCACGGCCATGGCGCCGTGGTGAGCCGTCCATGCCAGCGCTGCGAGATGGAGGCGAGGGCGCACCAGAAGATGGAGGACATGTCCGCCATGGAGAAGCTGCCCTCCTCGTAGGCAGGCATCGATGACTGCTTCCATCGCTTGCTACCTCACCAGCTCACCTGATAAATCACCTCAGTTACTATCGATTAATCATTTACCATGCATTAATTAAGAAGAAAACATATATACCATAATTATCTACCAGTAAAACAAATCTAATAGTAGTATTTAATAATCTCGTTGGTAATTAACTGCAGTCATGCATGAATGCATGCCATATTGCGTCGCTATCTTTTTTTCCCATGATGAGACTGATGAGAGAGAGAGAGAATATATGTCGCTATTGAGTGTTACTGCTTGATTACTGTACTAGTAGCTTATTACAATCAACCATTGTCTCTGTTGCGTATTTATGAGTGTAAGTGGTTAATTTTCTGTTGCAGCATAATTAAGTTACCTGTATATAATCTGATGTGTACGTACGCATACTGTCTCGATCGATCTCCGGGGCTTAAAAAGGAGATCGATTAATTGATCGAGGAGCAATTATAATTATGATGCAGTCATGTTTATAGTTAA
CDS Sequence
  • >LOC_Os07g48980.1
    ATGACGGTGGAAGTGGAGGCGGTGACCATGGCGAAGGAGGAGCAGCCGGAGGAGGAGGAGGTGATCGAGAAGTTGGTTGAGAAGATCACCGGGCTGGCGGCGGCCATCGGCAAGCTGCCGTCGCTGAGCCCGTCGCCGGAGGTGAACGCGCTGTTCACGGAGCTGGTGATGACCTGCATCCCGCCCAGCAGCGTGGACGTGGAGCAGCTGGGGGCGGAGGCGCAGGACATGCGCGGCCGCCTCATCCGCCTCTGCGCCGACGCCGAGGGCCACCTCGAGGCGCACTACTCCGACGTCCTCGCCGCCCACGACAACCCGCTCGACCACCTCGCCCTCTTCCCCTACTTCAACAACTACATCCAGCTCGCCCAGCTCGAGTACGCCCTCCTCGCCCGCCACCTCCCCGCCGCCCCGCCGCCCTCCCGCCTCGCCTTCCTCGGCTCCGGCCCGCTCCCGCTCAGCTCCCTCGTCCTCGCCGCCCGCCACCTCCCCGCCGCCTCCTTCCACAACTACGACATCTGCGCCGACGCCAACCGCCGCGCCAGCCGCCTCGTCCGCGCCGACCGCGACCTGTCCGCGCGCATGGCCTTCCACACCTCCGACGTCGCCCACGTCACCACCGACCTCGCCGCCTACGACGTCGTGTTCCTGGCGGCGCTGGTGGGTATGGCCGCCGAGGAGAAGGCGCGCATGGTGGAGCACCTCGGGAAGCACATGGCGCCCGGCGCCGCCCTGGTGGTGCGGAGCGCGCACGGCGCCCGGGGATTCCTGTACCCCGTGGTGGACCCGGAGGAGATCCGCCGCGGCGGCTTCGACGTGCTCGCCGTGCACCACCCGGAGGGCGAGGTGATCAACTCCGTCATCATCGCGCGCAAGCCGCCCGTGGCGGCGCCGGCGTTGGAGGGAGGAGACGCGCACGCGCACGGCCATGGCGCCGTGGTGAGCCGTCCATGCCAGCGCTGCGAGATGGAGGCGAGGGCGCACCAGAAGATGGAGGACATGTCCGCCATGGAGAAGCTGCCCTCCTCGTAG
Protein Sequence
  • >LOC_Os07g48980.1
    MTVEVEAVTMAKEEQPEEEEVIEKLVEKITGLAAAIGKLPSLSPSPEVNALFTELVMTCIPPSSVDVEQLGAEAQDMRGRLIRLCADAEGHLEAHYSDVLAAHDNPLDHLALFPYFNNYIQLAQLEYALLARHLPAAPPPSRLAFLGSGPLPLSSLVLAARHLPAASFHNYDICADANRRASRLVRADRDLSARMAFHTSDVAHVTTDLAAYDVVFLAALVGMAAEEKARMVEHLGKHMAPGAALVVRSAHGARGFLYPVVDPEEIRRGGFDVLAVHHPEGEVINSVIIARKPPVAAPALEGGDAHAHGHGAVVSRPCQRCEMEARAHQKMEDMSAMEKLPSS*