Gene Details:

• MSU gene ID: LOC_Os07g46460
• RAPdb gene ID: Os07g0658400
• Gene Symbol: ABC1 OsFd-GOGAT SPL32 ES7
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Metabolomics analysis revealed the accumulation of excessive amount of amino acids with high N/C ratio (Gln and Asn) and several intermediates in the tricarboxylic acid cycle in ABC1-1, suggesting that ABC1 plays a critical role in regulating nitrogen assimilation and carbon-nitrogen balance.
• The contribution of OsFd-GOGAT to rice foliar nitrogen metabolism remains little up-to-date.
• The results in this study suggested that OsFd-GOGAT might participate in nitrogen remobilization during leaf senescence, which provides a potential way to improve nitrogen use efficiency in rice.
• The SPL32 plants displayed early leaf senescence, identified by disintegration of chloroplasts as cellular evidence, dramatically decreased contents of chlorophyll, up-regulation of superoxide dismutase enzyme activity and malondialdehyde, as physiological characteristic, and both up-regulation of senescence-induced STAY GREEN gene and senescence-associated transcription factors, and down-regulation of photosynthesis-associated genes, as molecular indicators.
• qRT-PCR analysis indicated that several genes related to nitrogen metabolism were differentially expressed in ES7.
• In addition, when seedlings are grown under increasing nitrogen concentrations (NH4NO3) for 15 days, the contents of chlorophyll a, chlorophyll b and total chlorophyll were significantly lower in ES7.
• Our results demonstrated that ES7 is involved in nitrogen metabolism, effects chlorophyll synthesis, and may also associated with photorespiration, impacting leaf senescence in rice.
• ES7 was expressed constitutively, and the ES7 protein was localized in chloroplast.
• When ES7 plants are grown under photorespiration-suppressed conditions (high CO2), the senescence phenotype and chlorophyll content are rescued.
• The leaves of the ES7 mutant begin to senesce at the tillering stage about 60 day after sowing, and become increasingly senescent as the plants develop at the heading stage.

Function-related keywords:

• nitrogen ,  leaf ,  leaf-senescence ,  transcription-factor ,  early-leaf-senescence ,  seedlings ,  chloroplast ,  senescence ,  tillering

Literature:

• Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies .  DOI:  10.1016/j.molp.2016.09.004 ;  PMID:  27677460
• The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice .  DOI:  10.1007/s00438-016-1275-z ;  PMID:  28012016
• Isolation and characterization of a spotted leaf 32 mutant with early leaf senescence and enhanced defense response in rice .  DOI:  10.1038/srep41846 ;  PMID:  28139777
• ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice .  DOI:  10.1016/j.plantsci.2017.03.003 ;  PMID:  28483051
• Nitrogen Mediates Flowering Time and Nitrogen Use Efficiency via Floral Regulators in Rice .  DOI:  10.1016/j.cub.2020.10.095 ;  PMID:  33278354

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: