Gene Details:

Functional Descriptions:

  • We propose that NTRC constitutes an alternative system for chloroplast protection against oxidative damage, using NADPH as the source of reducing power.
  • Since no light-driven reduced ferredoxin is produced at night, the NTRC-BAS1 pathway may be a key detoxification system during darkness, with NADPH produced by the oxidative pentose phosphate pathway as the source of reducing power.
  • Rice NTRC is a high-efficiency redox system for chloroplast protection against oxidative damage.
  • An Arabidopsis thaliana knockout mutant for NTRC shows irregular mesophyll cell shape, abnormal chloroplast structure, and unbalanced BAS1 redox state, resulting in impaired photosynthesis rate under low light.
  • We show that rice (Oryza sativa) chloroplast NADPH THIOREDOXIN REDUCTASE (NTRC), with a thioredoxin domain, uses NADPH to reduce the chloroplast 2-Cys peroxiredoxin BAS1, which then reduces hydrogen peroxide.
  • Moreover, prolonged darkness followed by light/dark incubation produced an increase in hydrogen peroxide and lipid peroxidation in leaves and accelerated senescence of NTRC-deficient plants.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os07g46410.1
    CCATCCTCTCCCTCTCCTCTACTCCACACCCTCCGCTTCTCCACTCCTCCCTCGCGATGGCGGTCACGCGCCTCGCCGTGGCCGCCGCCCTCTCGGCCGCGCCGCCGTCCTCCCGCCGCCGCAGGGCCTTCTTCCATCACTCCTGCCGCCCGCTCCCTTCGTCGGCGGCGGCGGCGGCCAAGGCGTTGCGCGCGTCGGCTGCCCCCGCCGTCGACGAGGAGGCCCCCGCATCCCCGCCTCCATCAGATCTTGGCAAGGGAGTGGAGAACTTGGTGATCATAGGCTCTGGCCCGGCGGGGTACACTGCCGCGATTTACGCCGCTCGGGCGAACCTCAAGCCCGTCGTGTTCGAAGGCTACCAGGTTGGTGGCGTTCCTGGTGGGCAGCTGATGACCACCACCGAGGTGGAGAATTTCCCTGGGTTTCCGGATGGCGTCACTGGGCCTGATCTCATGGACAAAATGCGGAAGCAAGCGGAGCGGTGGGGTGCTGAGCTTCACCAGGAGGATGTTGAGTTTGTGAATGTGAAGAGCAGACCGTTTGTTATTCGTAGCAGTGATCGTGAGGTGAAATGCCATAGTGTAATCATTGCTACTGGTGCTGCTGCTAAGCGCCTTCGATTACCTCGTGAAGATGAATTTTGGAGTAGAGGTATCAGTGCATGTGCAATATGTGATGGAGCATCCCCACTATTTAAGGGTCAAGTTCTTGCAGTTGTTGGAGGTGGTGATACAGCTACAGAGGAAGCTATATATTTGACCAAATATGCACGTCACGTTCATTTACTTGTTCGAAAGGATCAGCTACGAGCATCCAAAGCTATGCAGGACCGAGTACTCAACAATCCCAACATAACAGTACATTTCAATACAGAAGCTGTGGATGTTGTCAGCAATCCCAAAGGCCAGATGTCTGGTATTCAACTGAAGAGAACCGACACGGGAGAGGAATCAGTTCTTGAGGTGAAAGGTCTATTCTATGGCATAGGACACACTCCAAACAGTCAGTTGTTACAAGGTCAAATTGATCTTGACGATGCTGGTTATATTTTGGTTGAAGAAGGCACAGCAAAAACATCAGTTGATGGTGTATTTGCTGCTGGTGATGTGCAGGATCATGAATGGAGGCAAGCCGTTACTGCAGCTGGATCCGGATGTGTAGCCGCTTTGTCGGTGGAAAGATACTTAGTTGCCAACGACCTTCTTGTTGAATTTCACCAGCCTGTTCGCGAAGAAAAGGAGAAGGAGATTACAGATAGAGATGTCGAGATGGGCTTTGACATTTCTCACACAAAGCATAGGGGACAGTATGCACTCCGCAAAGTATATCATGAAAGTCCAAGGCTTGTCTGTGTTCTATATACTTCTCCAACATGTGGTCCTTGCAGAACCTTAAAGCCAATTCTAAGCAAGGTTATAGATGAGTACAACGAACACGTTCATTTTGTCGAAATTGACATCGAGGAGGATCCTGAAATAGCAGAAGCTGCAGGCATCATGGGAACACCATGTGTTCAATTTTTTAAAAATAAAGAAATGCTCAGGACTGTCTCTGGTGTTAAAATGAAGAAGGAATATAGGGAGTTTATCGAGTCAAACAAATGAGCTGAACCCAACATACGATTCAAATTTTAAAGTGTAATAGTCTTCTTTTGCCCATACTGACTATTTCTAGCTGCCACCGTGGTGGCCAATTTTTGGTCAAAAATTTTCAGATGGGCCGGGTGTAGTGCGGTTCAAGAGGAAATTAGCCGGTCGCTCCTATTGAATTCAATCAATGTAACAGAATATATTCTTTTGCTGTACAACAACGTATTGGTCGTTGTCTTGACACCGTGCAAACTCCTCCCTGACAATATATACGTCTTAAAGATTTTGGAC
CDS Sequence
  • >LOC_Os07g46410.1
    ATGGCGGTCACGCGCCTCGCCGTGGCCGCCGCCCTCTCGGCCGCGCCGCCGTCCTCCCGCCGCCGCAGGGCCTTCTTCCATCACTCCTGCCGCCCGCTCCCTTCGTCGGCGGCGGCGGCGGCCAAGGCGTTGCGCGCGTCGGCTGCCCCCGCCGTCGACGAGGAGGCCCCCGCATCCCCGCCTCCATCAGATCTTGGCAAGGGAGTGGAGAACTTGGTGATCATAGGCTCTGGCCCGGCGGGGTACACTGCCGCGATTTACGCCGCTCGGGCGAACCTCAAGCCCGTCGTGTTCGAAGGCTACCAGGTTGGTGGCGTTCCTGGTGGGCAGCTGATGACCACCACCGAGGTGGAGAATTTCCCTGGGTTTCCGGATGGCGTCACTGGGCCTGATCTCATGGACAAAATGCGGAAGCAAGCGGAGCGGTGGGGTGCTGAGCTTCACCAGGAGGATGTTGAGTTTGTGAATGTGAAGAGCAGACCGTTTGTTATTCGTAGCAGTGATCGTGAGGTGAAATGCCATAGTGTAATCATTGCTACTGGTGCTGCTGCTAAGCGCCTTCGATTACCTCGTGAAGATGAATTTTGGAGTAGAGGTATCAGTGCATGTGCAATATGTGATGGAGCATCCCCACTATTTAAGGGTCAAGTTCTTGCAGTTGTTGGAGGTGGTGATACAGCTACAGAGGAAGCTATATATTTGACCAAATATGCACGTCACGTTCATTTACTTGTTCGAAAGGATCAGCTACGAGCATCCAAAGCTATGCAGGACCGAGTACTCAACAATCCCAACATAACAGTACATTTCAATACAGAAGCTGTGGATGTTGTCAGCAATCCCAAAGGCCAGATGTCTGGTATTCAACTGAAGAGAACCGACACGGGAGAGGAATCAGTTCTTGAGGTGAAAGGTCTATTCTATGGCATAGGACACACTCCAAACAGTCAGTTGTTACAAGGTCAAATTGATCTTGACGATGCTGGTTATATTTTGGTTGAAGAAGGCACAGCAAAAACATCAGTTGATGGTGTATTTGCTGCTGGTGATGTGCAGGATCATGAATGGAGGCAAGCCGTTACTGCAGCTGGATCCGGATGTGTAGCCGCTTTGTCGGTGGAAAGATACTTAGTTGCCAACGACCTTCTTGTTGAATTTCACCAGCCTGTTCGCGAAGAAAAGGAGAAGGAGATTACAGATAGAGATGTCGAGATGGGCTTTGACATTTCTCACACAAAGCATAGGGGACAGTATGCACTCCGCAAAGTATATCATGAAAGTCCAAGGCTTGTCTGTGTTCTATATACTTCTCCAACATGTGGTCCTTGCAGAACCTTAAAGCCAATTCTAAGCAAGGTTATAGATGAGTACAACGAACACGTTCATTTTGTCGAAATTGACATCGAGGAGGATCCTGAAATAGCAGAAGCTGCAGGCATCATGGGAACACCATGTGTTCAATTTTTTAAAAATAAAGAAATGCTCAGGACTGTCTCTGGTGTTAAAATGAAGAAGGAATATAGGGAGTTTATCGAGTCAAACAAATGA
Protein Sequence
  • >LOC_Os07g46410.1
    MAVTRLAVAAALSAAPPSSRRRRAFFHHSCRPLPSSAAAAAKALRASAAPAVDEEAPASPPPSDLGKGVENLVIIGSGPAGYTAAIYAARANLKPVVFEGYQVGGVPGGQLMTTTEVENFPGFPDGVTGPDLMDKMRKQAERWGAELHQEDVEFVNVKSRPFVIRSSDREVKCHSVIIATGAAAKRLRLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKYARHVHLLVRKDQLRASKAMQDRVLNNPNITVHFNTEAVDVVSNPKGQMSGIQLKRTDTGEESVLEVKGLFYGIGHTPNSQLLQGQIDLDDAGYILVEEGTAKTSVDGVFAAGDVQDHEWRQAVTAAGSGCVAALSVERYLVANDLLVEFHQPVREEKEKEITDRDVEMGFDISHTKHRGQYALRKVYHESPRLVCVLYTSPTCGPCRTLKPILSKVIDEYNEHVHFVEIDIEEDPEIAEAAGIMGTPCVQFFKNKEMLRTVSGVKMKKEYREFIESNK*