GE;CYP78A13

Gene Details

MSU gene ID: LOC_Os07g41240
RAPdb gene ID: Os07g0603700
Gene Symbol: GE CYP78A13
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Overexpression of GE promoted cell proliferation and enhanced rice plant growth and grain yield, but reduced embryo size, sugGEsting that GE is critical for coordinating rice embryo and endosperm development.
The function of GE in each tissue is distinct, controlling cell size in the embryo and cell death in the endosperm.
Post-embryonic growth of GE seedling was severely inhibited due to defective shoot apical meristem (SAM) maintenance.
GE, which encodes CYP78A13, is predominantly expressed in the interfacing tissues of the both embryo and endosperm.
In contrast to the loss-of-function mutant with larGE embryo and small endosperm, GE overexpression causes a small embryo and enlarGEd endosperm.
A complementation analysis coupled with heterofertilization showed that complementation of GE mutation in either embryo or endosperm failed to restore the wild-type embryo/endosperm ratio.
Among GEnes associated with embryo/endosperm size, REDUCED EMBRYO GEnes, whose loss-of-function causes a phenotype opposite to GE, are revealed to regulate endosperm size upstream of GE.
To fully understand the embryo-endosperm size control, the GEnetic network of the related GEnes should be elucidated.
However, the molecular mechanism(s) underlying embryo and endosperm development remains larGEly unknown, particularly in rice, the model cereal.
GE is expressed predominantly in the scutellar epithelium, the interface region between embryo and endosperm.
Mutation of GE resulted in a larGE embryo in the seed, which was caused by excessive expansion of scutellum cells.
Moreover, transGEnic Arabidopsis plants overexpressing AtCYP78A10, a GE homolog, also produced bigGEr seeds, implying a conserved role for the CYP78A subfamily of P450s in regulating seed development.
2 showed that both can promote grain growth.
Variations in CYP78A13 coding region influence grain size and yield in rice.
Grain size is one of the most important determinants of crop yield in cereals.
Here, we identified a dominant mutant, big grain2 (bg2-D) from our enhancer-trapping population.
Sequence search revealed that CYP78A13 has a paralogue Grain<U+2005>Length 3.
Detailed examination of the transgenic plants with chimaeric constructs suggested that variation in CYP78A13 coding region is responsible for the variation of grain yield.
Taken together, our results suggest that the variations in CYP78A13 in the indica varieties hold potential in rice breeding for application of grain yield improvement.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os07g41240.1
ACGTCTTCTTCCTCCCAAGAAAACTCCTCTCACTTCGCGAACGCTTCCCATGGCGCTCTCCTCCATGGCCGCGGCGCAAGAGAGCTCCCTCCTCCTCTTCCTCCTCCCGACGTCGGCCGCCTCCGTGTTCCCGCCGCTCATCTCCGTGGTCGTCCTCGCCGCGCTCCTCCTGTGGCTCTCGCCGGGTGGCCCCGCGTGGGCGCTGTCCCGTTGCCGTGGCACGCCGCCGCCGCCGGGCGTGGCGGGGGGCGCGGCCAGCGCGCTGTCCGGCCCTGCCGCGCACCGCGTGCTCGCCGGGATTTCGCGCGCCGTCGAGGGCGGCGCGGCGGTGATGTCGCTCTCCGTCGGCCTCACCCGCCTCGTCGTGGCGAGCCGGCCGGAGACGGCGAGGGAGATCCTCGTCAGCCCGGCGTTCGGCGACCGCCCCGTGAAGGACGCGGCGAGGCAGCTGCTGTTCCACCGCGCCATGGGGTTCGCCCCGTCGGGCGACGCGCACTGGCGCGGGCTCCGCCGCGCCTCCGCGGCGCACCTCTTCGGCCCGCGCCGCGTGGCCGGGTCCGCGCCCGAGCGCGAGGCCATCGGCGCCCGCATAGTCGGCGACGTCGCCTCCCTCATGTCCCGCCGCGGCGAGGTCCCCCTCCGCCGCGTCCTTCACGCCGCGTCGCTCGGCCACGTCATGGCGACCGTCTTCGGCAAGCGGCACGGCGACATCTCGATCCAGGACGGCGAGCTCCTGGAGGAGATGGTCACCGAAGGGTACGACCTCCTCGGCAAGTTCAACTGGGCCGACCACCTGCCATTGCTCAGGTGGCTCGACCTCCAGGGCATCCGCCGCCGGTGCAACAGGCTAGTCCAGAAGGTGGAGGTGTTCGTCGGAAAGATCATACAGGAGCACAAGGCGAAGCGAGCTGCCGGAGGCGTCGCCGTCGCCGACGGCGTCTTGGGCGACTTCGTCGACGTCCTCCTCGACCTCCAGGGAGAGGAGAAGATGTCAGACTCCGACATGATCGCTGTTCTTTGGGAGATGATCTTTAGAGGGACGGACACGGTGGCGATCTTGATGGAGTGGGTGATGGCGAGGATGGTGATGCACCCGGAGATCCAGGCGAAGGCGCAGGCGGAGGTGGACGCCGCCGTGGGGGGACGCCGCGGCCGCGTCGCCGACGGCGACGTGGCGAGCCTCCCCTACATCCAGTCCATCGTGAAGGAGACGCTGCGCATGCACCCGCCGGGCCCGCTCCTGTCGTGGGCGCGCCTCGCCGTGCACGACGCGCGCGTCGGTGGCCACGCCGTCCCCGCCGGGACGACGGCGATGGTGAACATGTGGGCGATCGCCCACGACGCCGCCGTCTGGCCGGAGCCGGATGCGTTCCGCCCGGAGCGCTTCTCGGAGGGGGAGGACGTCGGCGTGCTCGGCGGCGACCTCCGCCTCGCGCCGTTCGGCGCCGGCCGCCGCGTCTGCCCTGGCAGGATGCTGGCGCTCGCCACCGCCCACCTCTGGCTCGCCCAGCTGCTGCACGCCTTCGACTGGTCCCCCACCGCCGCCGGCGTCGACCTGTCCGAGCGCCTCGGCATGTCGCTGGAGATGGCGGCGCCGCTCGTGTGCAAGGCCGTGGCTAGGGCCTGAGCCCTAGCCGCCGCCGCCGCCATTATTGCCATTGATGTGGCTAGCGACGTTGTCGTGCTCGCATCCATACTCCTCCATAGGCAACTCGTCTAGCCAATGAAGAAAGCTACTATCTATCTATCTATCAAGCTAGCTGCTACTATCACAAACCGCATTTCGGCATCATCTTAAATTAGCTCTTAGGGGTGTAGGCGATTTTGGTTTCCCCCAAAAATTTGCTTTGCCAGTCTTTTGGTTTAAATCGAGGCATTAGTTGTGAAACATCATGAGAAGTTATTTAAATCTGAGGAATTTTGTTTGAACCTTTTCTGGTGTGCT

CDS Sequence

>LOC_Os07g41240.1
ATGGCGCTCTCCTCCATGGCCGCGGCGCAAGAGAGCTCCCTCCTCCTCTTCCTCCTCCCGACGTCGGCCGCCTCCGTGTTCCCGCCGCTCATCTCCGTGGTCGTCCTCGCCGCGCTCCTCCTGTGGCTCTCGCCGGGTGGCCCCGCGTGGGCGCTGTCCCGTTGCCGTGGCACGCCGCCGCCGCCGGGCGTGGCGGGGGGCGCGGCCAGCGCGCTGTCCGGCCCTGCCGCGCACCGCGTGCTCGCCGGGATTTCGCGCGCCGTCGAGGGCGGCGCGGCGGTGATGTCGCTCTCCGTCGGCCTCACCCGCCTCGTCGTGGCGAGCCGGCCGGAGACGGCGAGGGAGATCCTCGTCAGCCCGGCGTTCGGCGACCGCCCCGTGAAGGACGCGGCGAGGCAGCTGCTGTTCCACCGCGCCATGGGGTTCGCCCCGTCGGGCGACGCGCACTGGCGCGGGCTCCGCCGCGCCTCCGCGGCGCACCTCTTCGGCCCGCGCCGCGTGGCCGGGTCCGCGCCCGAGCGCGAGGCCATCGGCGCCCGCATAGTCGGCGACGTCGCCTCCCTCATGTCCCGCCGCGGCGAGGTCCCCCTCCGCCGCGTCCTTCACGCCGCGTCGCTCGGCCACGTCATGGCGACCGTCTTCGGCAAGCGGCACGGCGACATCTCGATCCAGGACGGCGAGCTCCTGGAGGAGATGGTCACCGAAGGGTACGACCTCCTCGGCAAGTTCAACTGGGCCGACCACCTGCCATTGCTCAGGTGGCTCGACCTCCAGGGCATCCGCCGCCGGTGCAACAGGCTAGTCCAGAAGGTGGAGGTGTTCGTCGGAAAGATCATACAGGAGCACAAGGCGAAGCGAGCTGCCGGAGGCGTCGCCGTCGCCGACGGCGTCTTGGGCGACTTCGTCGACGTCCTCCTCGACCTCCAGGGAGAGGAGAAGATGTCAGACTCCGACATGATCGCTGTTCTTTGGGAGATGATCTTTAGAGGGACGGACACGGTGGCGATCTTGATGGAGTGGGTGATGGCGAGGATGGTGATGCACCCGGAGATCCAGGCGAAGGCGCAGGCGGAGGTGGACGCCGCCGTGGGGGGACGCCGCGGCCGCGTCGCCGACGGCGACGTGGCGAGCCTCCCCTACATCCAGTCCATCGTGAAGGAGACGCTGCGCATGCACCCGCCGGGCCCGCTCCTGTCGTGGGCGCGCCTCGCCGTGCACGACGCGCGCGTCGGTGGCCACGCCGTCCCCGCCGGGACGACGGCGATGGTGAACATGTGGGCGATCGCCCACGACGCCGCCGTCTGGCCGGAGCCGGATGCGTTCCGCCCGGAGCGCTTCTCGGAGGGGGAGGACGTCGGCGTGCTCGGCGGCGACCTCCGCCTCGCGCCGTTCGGCGCCGGCCGCCGCGTCTGCCCTGGCAGGATGCTGGCGCTCGCCACCGCCCACCTCTGGCTCGCCCAGCTGCTGCACGCCTTCGACTGGTCCCCCACCGCCGCCGGCGTCGACCTGTCCGAGCGCCTCGGCATGTCGCTGGAGATGGCGGCGCCGCTCGTGTGCAAGGCCGTGGCTAGGGCCTGA

Protein Sequence

>LOC_Os07g41240.1
MALSSMAAAQESSLLLFLLPTSAASVFPPLISVVVLAALLLWLSPGGPAWALSRCRGTPPPPGVAGGAASALSGPAAHRVLAGISRAVEGGAAVMSLSVGLTRLVVASRPETAREILVSPAFGDRPVKDAARQLLFHRAMGFAPSGDAHWRGLRRASAAHLFGPRRVAGSAPEREAIGARIVGDVASLMSRRGEVPLRRVLHAASLGHVMATVFGKRHGDISIQDGELLEEMVTEGYDLLGKFNWADHLPLLRWLDLQGIRRRCNRLVQKVEVFVGKIIQEHKAKRAAGGVAVADGVLGDFVDVLLDLQGEEKMSDSDMIAVLWEMIFRGTDTVAILMEWVMARMVMHPEIQAKAQAEVDAAVGGRRGRVADGDVASLPYIQSIVKETLRMHPPGPLLSWARLAVHDARVGGHAVPAGTTAMVNMWAIAHDAAVWPEPDAFRPERFSEGEDVGVLGGDLRLAPFGAGRRVCPGRMLALATAHLWLAQLLHAFDWSPTAAGVDLSERLGMSLEMAAPLVCKAVARA*