Gene Details:

• MSU gene ID: LOC_Os07g40510 ,LOC_Os07g40520
• RAPdb gene ID: Os07g0596300
• Gene Symbol: FH5 RMD BUI1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• FH5/RMD is ubiquitously expressed in rice tissues, and its protein localization to the chloroplast surface is mediated by the PTEN domain.
• The RMD mutants display a bending growth pattern in seedlings, are stunted as adult plants, and have aberrant inflorescence (panicle) and seed shape.
• Cytological observation indicated that the growth defects of BUI1 were caused mainly by inhibition of cell expansion.
• The BUI1 mutant displayed pleiotropic phenotypes, including bent uppermost internode, dwarfism, wavy panicle rachis, and enhanced gravitropic response.
• Cytological analysis showed that RMD mutants have severe cell elongation defects and abnormal microtubule and microfilament arrays.
• The rice RMD mutant exhibits abnormal pollen tube growth and a decreased germination rate of the pollen grain in vitro and in vivo.
• The RMD pollen tubes display a disorganized F-actin pattern with disrupted apical actin density and shank longitudinal cable direction/arrangement, indicating the novel role of RMD in F-actin polarity during tip growth.
• Consistent with this role, RMD localizes at the tip of the rice pollen tube, which is essential for pollen tube growth and polarity as well as F-actin organization.
• Collectively, our results suggest that RMD is essential for the spatial regulation in pollen tube growth via modulating F-actin organization and array orientation in rice.
• RMD protein localizes to the surface of statoliths, and RMD mutants exhibit faster gravitropic response owing to more rapid statoliths movement.
• Rice actin binding protein RMD controls crown root angle in response to external phosphate.
• RMD is upregulated in response to low external phosphate and mutants lacking of RMD have steeper crown root growth angles that are unresponsive to phosphate levels.
• We conclude that adaptive changes to root angle in response to external phosphate availability are RMD dependent, providing a potential target for breeders.
• The rice actin-binding protein RMD regulates light-dependent shoot gravitropism.
• By contrast, etiolated RMD shoots displayed normal negative shoot gravitropism.
• Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots.

Function-related keywords:

• chloroplast ,  panicle ,  growth ,  seed ,  dwarf ,  seedling ,  inflorescence ,  cell-elongation ,  pollen ,  grain ,  gravitropic-response ,  root ,  crown ,  crown-root ,  phosphate ,  shoot-gravitropism ,  shoot ,  auxin

Literature:

• BENT UPPERMOST INTERNODE1 encodes the class II formin FH5 crucial for actin organization and rice development .  DOI:  10.1105/tpc.110.081802 ;  PMID:  21307285
• RICE MORPHOLOGY DETERMINANT encodes the type II formin FH5 and regulates rice morphogenesis .  DOI:  10.1105/tpc.110.081349 ;  PMID:  21307283
• Rice actin-binding protein RMD is a key link in the auxin-actin regulatory loop that controls cell growth .  DOI:  10.1073/pnas.1401680111 ;  PMID:  24982173
• Rice Morphology Determinant-Mediated Actin Filament Organization Contributes to Pollen Tube Growth .  DOI:  10.1104/pp.17.01759 ;  PMID:  29581178
• Rice actin binding protein RMD controls crown root angle in response to external phosphate .  DOI:  10.1038/s41467-018-04710-x ;  PMID:  29892032
• The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism .  DOI:  10.1104/pp.19.00497 ;  PMID:  31416828

Related News:

Gene Resources:

• NCBI ID: HQ123580  , AK120222
• UniProt accessions:

Sequences: