Information report for OsGH3.8;OsGH3-8
Gene Details
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Functional Descriptions
- OsMGH3/OsGH3.8 is expressed abundantly in rice florets and is regulated by two related and redundant transcription factors, OsMADS1 and OsMADS6, but its contribution to flower development is not known.
- Here we use GH3-8 from Oryza sativa (rice; OsGH3.8), which functions as an indole-acetic acid (IAA)-amido synthetase, for detailed mechanistic studies.
- The overexpression of OsMGH3 during the vegetative phase affects the overall plant architecture, whereas its inflorescence-specific overexpression creates short panicles with reduced branching, resembling in part the effects of OsMADS1 overexpression.
- Florets in OsMGH3 knock-down plants were affected in carpel development and pollen viability, both of which reduced fertility.
- Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility.
- Steady-state kinetic analysis shows that the OsGH3.8 requires either Mg(2+) or Mn(2+) for maximal activity and is specific for aspartate but accepts asparagine as a substrate with a 45-fold decrease in catalytic efficiency and accepts other auxin analogs, including phenyl-acetic acid, indole butyric acid, and naphthalene-acetic acid, as acyl-acid substrates with 1.
- In contrast, the down-regulation of endogenous OsMGH3 caused phenotypes consistent with auxin overproduction or activated signaling, such as ectopic rooting from aerial nodes.
- Through a screen for OsMADS1 targets we identify a flower-specific Nt-gh3 type gene, OsMGH3, as a downstream gene.
- Reduced miR167 levels or overexpressing OsGH3.8 increase auxin metabolism, reduce endogenous levels of free IAA and enhance rice AG tolerance.
- The OsGH3.8 encodes an IAA-amido synthetase that functions to prevent free IAA accumulation.
Functional Keywords
- transcription-factor , iaa , branching , fertility , vegetative , auxin , flower , panicle , inflorescence , root , pollen , architecture , tolerance , IAA
Literature and News
- The auxin-responsive GH3 gene family in rice (Oryza sativa) . DOI: 10.1007/s10142-005-0142-5 ; PMID: 15856348
- Kinetic basis for the conjugation of auxin by a GH3 family indole-acetic acid-amido synthetase . DOI: 10.1074/jbc.M110.146431 ; PMID: 20639576
- OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs . DOI: 10.1111/j.1365-313X.2005.02504.x ; PMID: 16146529
- Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice . DOI: 10.1105/tpc.107.055657 ; PMID: 18192436
- Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility . DOI: 10.1093/pcp/pcr142 ; PMID: 22016342
- Gene Expression Dynamics in Rice Peduncles at the Heading Stage . DOI: 10.3389/fgene.2020.584678 ; PMID: 33343630
- Identification of a novel QTL and candidate gene associated with grain size using chromosome segment substitution lines in rice . DOI: 10.1038/s41598-020-80667-6 ; PMID: 33420305
- Auxin plays a role in the adaptation of rice to anaerobic germination and seedling establishment . DOI: 10.1111/pce.14434 ; PMID: 36071575
Gene Resources
- UniProt: Q0D4Z6
- EMBL: AK101193, AP003846, AP008213
- AlphaFoldDB: Q0D4Z6
- EnsemblPlants: Os07t0592600-01
- Gramene: Os07t0592600-01
- KEGG: dosa:Os07g0592600
- Orthologous matrix: EGFFAIQ
- InterPro: IPR004993, IPR055377, IPR055378
- PANTHER: PTHR31901, PTHR31901:SF96
- OrthoDB: Q0D4Z6
- SWISS-MODEL: Q0D4Z6
- eggNOG: ENOG502QR80
Sequences
cDNA Sequence
- >LOC_Os07g40290.1
GACTCAGGCCACACACCACCAACACAAAACATTTCCTTGTCCCTCGCCTTCCTCGCCTCGCCTCGGCTCTCACACGACGCCTCTTCCTCTCGCTACTACTAGCTCAAGCTAAGCTAGCAAGAGCATTCCTTGGGCTTTTTTTCTTGTTCGGGGTTGAGAGGCAATGGCGGTGATGACTGATGTGTCGACCACCGGGACGGCACTCCGGACCCCGGCGGCGGGGGCGGTGAAGGAGGGCGACGTCGAGAAGCTCCGGTTCATCGACGAGATGACCACCAACGTCGACGCCGTGCAGGAGCGCGTCCTGGGGGAGATCCTCGGCCGCAACGCCGGCACGGAGTACCTGACCAAGTGCGGCCTCGACGGCGCCACCGACCGCGCCGCCTTCCGGGCCAAGGTTCCGGTGGTGTCGTACGACGACCTGCAGCCGTACATCCAGCGCATTGCGAACGGGGACCGCTCGCCGATCCTGTCCACCCACCCCGTCTCCGAGTTCCTCACCAGCTCCGGCACGTCGGCCGGCGAGCGCAAGCTGATGCCCACCATCATGGACGAGCTCGACCGCCGTCAGCTGCTCTACAGCCTCCTCATGCCGGTCATGAACTTGTATGTGCCTGGGCTTGACAAGGGGAAGGGGCTCTACTTCCTGTTCGTCAAGTCGGAGACGAAGACGCCGGGAGGCCTGACGGCGAGGCCCGTGCTGACGAGCTACTACAAGAGCGACCACTTCAAGAACCGGCCGTACGACCCGTACCACAACTACACGAGCCCGACGGCGGCCATCCTCTGCGCCGACGCGTTCCAGAGCATGTACGCGCAGATGGTGTGCGGCCTGTGCCAGCGCAACGACGTGCTGCGCCTCGGCGCCGTGTTCGCCTCCGGCCTCCTCCGGGCCATCCGCTTCCTCCAGCTCAACTGGGAGCAGCTCGCCGACGACATCGAGTCCGGCGAGCTCACCCCTCGCGTCACCGACCCGTCGGTGCGCGAGGCTGTCGCCGCCATCCTCCTCCCGGACCCCGAGCTCGCCAAGCTCATCCGCGCCGAGTGCTCCAAGGGCGACTGGGCCGGCATCATCACCCGCGTGTGGCCGAACACCAAGTACCTCGACGTCATCGTCACCGGCGCGATGGCGCAGTACATCCCGACCCTGGAGTTCTACAGCGGCGGGCTTCCCATGGCGTGCACCATGTACGCGTCATCCGAGTGCTACTTCGGCCTCAACCTCCGCCCCATGTGCGACCCCTCCGAGGTGTCCTACACCATCATGCCTAACATGGGCTACTTCGAGTTCCTCCCCGTGGACGAGACCGGCGCGGCTTCGGGCGACGCCACCCAGCTCGTGGACCTCGCCCGCGTGGAGGTGGGGCGCGAGTACGAGCTGGTGATCACCACCTACGCCGGGCTGAACCGGTACCGCGTCGGCGACGTCCTCCGCGTGACGGGGTTCCACAACGCGGCGCCGCAGTTCAGGTTCGTGCGCCGCAAGAACGTGCTCCTCTCCATCGAGTCCGACAAGACCGACGAGGCCGAGCTGCAGCGCGCCGTGGAGCGCGCGTCGGCGCTGCTCCGCCCGCACGGCGCGTCCGTGGTGGAGTACACCAGCCAAGCGTGCACCAAGCGCATCCCGGGCCACTACGTCATCTACTGGGAGCTCCTGACCAAGGGCGCCGGCGCCACGGTGGTGGACGCGGACACGCTGGGCAGGTGCTGCCTCGAGATGGAGGAGGCGCTGAACACGGTGTACAGGCAGAGCCGCGTCGCGGACGGCTCGATTGGGCCGCTCGAGATCCGGGTCGTCCGCCCGGGCACATTCGAGGAGCTCATGGACTACGCCATCTCCCGCGGCGCGTCCATCAACCAGTACAAGGTGCCACGCTGCGTCACGTTCCCGCCCATCGTCGAGCTGCTCGACTCGCGCGTCGTGTCCAGCCACTTCAGCCCGGCGCTCCCACACTGGACTCCGGCGCGACGGTCCGAATAGTCGGTCAAATCCCCACCTCGTCGTTGGACCGTGTCCAAGAATCTGAAGTAGTAGAAGCCGGATGCCTCCACCTAAAAACACTTATCTGTTATTTTTGGAATTAATTTTGATGGTTGCTGTCAACTCTGTCAGTTAGTGGCAAGAGGGTACCTCTGATGTGAGGGAGAGAACTGCAGAACGAACGGAAGAAAGTGTTGATGTAAGAGGTTACCACTAGTAGTACTGTTTGTCTGTATCAGGAATGTGATTATAATTTAACCTGTCCTCCAAAACCGTACGAGTCCTGCGCAAGATCTTGTTCCAATTATTGGCCAGACCTGGACAGCTTTTAGGCTGTGTTCGGCACAGCACTTTCA
CDS Sequence
- >LOC_Os07g40290.1
ATGGCGGTGATGACTGATGTGTCGACCACCGGGACGGCACTCCGGACCCCGGCGGCGGGGGCGGTGAAGGAGGGCGACGTCGAGAAGCTCCGGTTCATCGACGAGATGACCACCAACGTCGACGCCGTGCAGGAGCGCGTCCTGGGGGAGATCCTCGGCCGCAACGCCGGCACGGAGTACCTGACCAAGTGCGGCCTCGACGGCGCCACCGACCGCGCCGCCTTCCGGGCCAAGGTTCCGGTGGTGTCGTACGACGACCTGCAGCCGTACATCCAGCGCATTGCGAACGGGGACCGCTCGCCGATCCTGTCCACCCACCCCGTCTCCGAGTTCCTCACCAGCTCCGGCACGTCGGCCGGCGAGCGCAAGCTGATGCCCACCATCATGGACGAGCTCGACCGCCGTCAGCTGCTCTACAGCCTCCTCATGCCGGTCATGAACTTGTATGTGCCTGGGCTTGACAAGGGGAAGGGGCTCTACTTCCTGTTCGTCAAGTCGGAGACGAAGACGCCGGGAGGCCTGACGGCGAGGCCCGTGCTGACGAGCTACTACAAGAGCGACCACTTCAAGAACCGGCCGTACGACCCGTACCACAACTACACGAGCCCGACGGCGGCCATCCTCTGCGCCGACGCGTTCCAGAGCATGTACGCGCAGATGGTGTGCGGCCTGTGCCAGCGCAACGACGTGCTGCGCCTCGGCGCCGTGTTCGCCTCCGGCCTCCTCCGGGCCATCCGCTTCCTCCAGCTCAACTGGGAGCAGCTCGCCGACGACATCGAGTCCGGCGAGCTCACCCCTCGCGTCACCGACCCGTCGGTGCGCGAGGCTGTCGCCGCCATCCTCCTCCCGGACCCCGAGCTCGCCAAGCTCATCCGCGCCGAGTGCTCCAAGGGCGACTGGGCCGGCATCATCACCCGCGTGTGGCCGAACACCAAGTACCTCGACGTCATCGTCACCGGCGCGATGGCGCAGTACATCCCGACCCTGGAGTTCTACAGCGGCGGGCTTCCCATGGCGTGCACCATGTACGCGTCATCCGAGTGCTACTTCGGCCTCAACCTCCGCCCCATGTGCGACCCCTCCGAGGTGTCCTACACCATCATGCCTAACATGGGCTACTTCGAGTTCCTCCCCGTGGACGAGACCGGCGCGGCTTCGGGCGACGCCACCCAGCTCGTGGACCTCGCCCGCGTGGAGGTGGGGCGCGAGTACGAGCTGGTGATCACCACCTACGCCGGGCTGAACCGGTACCGCGTCGGCGACGTCCTCCGCGTGACGGGGTTCCACAACGCGGCGCCGCAGTTCAGGTTCGTGCGCCGCAAGAACGTGCTCCTCTCCATCGAGTCCGACAAGACCGACGAGGCCGAGCTGCAGCGCGCCGTGGAGCGCGCGTCGGCGCTGCTCCGCCCGCACGGCGCGTCCGTGGTGGAGTACACCAGCCAAGCGTGCACCAAGCGCATCCCGGGCCACTACGTCATCTACTGGGAGCTCCTGACCAAGGGCGCCGGCGCCACGGTGGTGGACGCGGACACGCTGGGCAGGTGCTGCCTCGAGATGGAGGAGGCGCTGAACACGGTGTACAGGCAGAGCCGCGTCGCGGACGGCTCGATTGGGCCGCTCGAGATCCGGGTCGTCCGCCCGGGCACATTCGAGGAGCTCATGGACTACGCCATCTCCCGCGGCGCGTCCATCAACCAGTACAAGGTGCCACGCTGCGTCACGTTCCCGCCCATCGTCGAGCTGCTCGACTCGCGCGTCGTGTCCAGCCACTTCAGCCCGGCGCTCCCACACTGGACTCCGGCGCGACGGTCCGAATAG
Protein Sequence
- >LOC_Os07g40290.1
MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRSE*