Gene Details:

Functional Descriptions:

  • OsMGH3/OsGH3.8 is expressed abundantly in rice florets and is regulated by two related and redundant transcription factors, OsMADS1 and OsMADS6, but its contribution to flower development is not known.
  • Here we use GH3-8 from Oryza sativa (rice; OsGH3.8), which functions as an indole-acetic acid (IAA)-amido synthetase, for detailed mechanistic studies.
  • The overexpression of OsMGH3 during the vegetative phase affects the overall plant architecture, whereas its inflorescence-specific overexpression creates short panicles with reduced branching, resembling in part the effects of OsMADS1 overexpression.
  • Florets in OsMGH3 knock-down plants were affected in carpel development and pollen viability, both of which reduced fertility.
  • Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility.
  • Steady-state kinetic analysis shows that the OsGH3.8 requires either Mg(2+) or Mn(2+) for maximal activity and is specific for aspartate but accepts asparagine as a substrate with a 45-fold decrease in catalytic efficiency and accepts other auxin analogs, including phenyl-acetic acid, indole butyric acid, and naphthalene-acetic acid, as acyl-acid substrates with 1.
  • In contrast, the down-regulation of endogenous OsMGH3 caused phenotypes consistent with auxin overproduction or activated signaling, such as ectopic rooting from aerial nodes.
  • Through a screen for OsMADS1 targets we identify a flower-specific Nt-gh3 type gene, OsMGH3, as a downstream gene.
  • Reduced miR167 levels or overexpressing OsGH3.8 increase auxin metabolism, reduce endogenous levels of free IAA and enhance rice AG tolerance.
  • The OsGH3.8 encodes an IAA-amido synthetase that functions to prevent free IAA accumulation.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os07g40290.1
    GACTCAGGCCACACACCACCAACACAAAACATTTCCTTGTCCCTCGCCTTCCTCGCCTCGCCTCGGCTCTCACACGACGCCTCTTCCTCTCGCTACTACTAGCTCAAGCTAAGCTAGCAAGAGCATTCCTTGGGCTTTTTTTCTTGTTCGGGGTTGAGAGGCAATGGCGGTGATGACTGATGTGTCGACCACCGGGACGGCACTCCGGACCCCGGCGGCGGGGGCGGTGAAGGAGGGCGACGTCGAGAAGCTCCGGTTCATCGACGAGATGACCACCAACGTCGACGCCGTGCAGGAGCGCGTCCTGGGGGAGATCCTCGGCCGCAACGCCGGCACGGAGTACCTGACCAAGTGCGGCCTCGACGGCGCCACCGACCGCGCCGCCTTCCGGGCCAAGGTTCCGGTGGTGTCGTACGACGACCTGCAGCCGTACATCCAGCGCATTGCGAACGGGGACCGCTCGCCGATCCTGTCCACCCACCCCGTCTCCGAGTTCCTCACCAGCTCCGGCACGTCGGCCGGCGAGCGCAAGCTGATGCCCACCATCATGGACGAGCTCGACCGCCGTCAGCTGCTCTACAGCCTCCTCATGCCGGTCATGAACTTGTATGTGCCTGGGCTTGACAAGGGGAAGGGGCTCTACTTCCTGTTCGTCAAGTCGGAGACGAAGACGCCGGGAGGCCTGACGGCGAGGCCCGTGCTGACGAGCTACTACAAGAGCGACCACTTCAAGAACCGGCCGTACGACCCGTACCACAACTACACGAGCCCGACGGCGGCCATCCTCTGCGCCGACGCGTTCCAGAGCATGTACGCGCAGATGGTGTGCGGCCTGTGCCAGCGCAACGACGTGCTGCGCCTCGGCGCCGTGTTCGCCTCCGGCCTCCTCCGGGCCATCCGCTTCCTCCAGCTCAACTGGGAGCAGCTCGCCGACGACATCGAGTCCGGCGAGCTCACCCCTCGCGTCACCGACCCGTCGGTGCGCGAGGCTGTCGCCGCCATCCTCCTCCCGGACCCCGAGCTCGCCAAGCTCATCCGCGCCGAGTGCTCCAAGGGCGACTGGGCCGGCATCATCACCCGCGTGTGGCCGAACACCAAGTACCTCGACGTCATCGTCACCGGCGCGATGGCGCAGTACATCCCGACCCTGGAGTTCTACAGCGGCGGGCTTCCCATGGCGTGCACCATGTACGCGTCATCCGAGTGCTACTTCGGCCTCAACCTCCGCCCCATGTGCGACCCCTCCGAGGTGTCCTACACCATCATGCCTAACATGGGCTACTTCGAGTTCCTCCCCGTGGACGAGACCGGCGCGGCTTCGGGCGACGCCACCCAGCTCGTGGACCTCGCCCGCGTGGAGGTGGGGCGCGAGTACGAGCTGGTGATCACCACCTACGCCGGGCTGAACCGGTACCGCGTCGGCGACGTCCTCCGCGTGACGGGGTTCCACAACGCGGCGCCGCAGTTCAGGTTCGTGCGCCGCAAGAACGTGCTCCTCTCCATCGAGTCCGACAAGACCGACGAGGCCGAGCTGCAGCGCGCCGTGGAGCGCGCGTCGGCGCTGCTCCGCCCGCACGGCGCGTCCGTGGTGGAGTACACCAGCCAAGCGTGCACCAAGCGCATCCCGGGCCACTACGTCATCTACTGGGAGCTCCTGACCAAGGGCGCCGGCGCCACGGTGGTGGACGCGGACACGCTGGGCAGGTGCTGCCTCGAGATGGAGGAGGCGCTGAACACGGTGTACAGGCAGAGCCGCGTCGCGGACGGCTCGATTGGGCCGCTCGAGATCCGGGTCGTCCGCCCGGGCACATTCGAGGAGCTCATGGACTACGCCATCTCCCGCGGCGCGTCCATCAACCAGTACAAGGTGCCACGCTGCGTCACGTTCCCGCCCATCGTCGAGCTGCTCGACTCGCGCGTCGTGTCCAGCCACTTCAGCCCGGCGCTCCCACACTGGACTCCGGCGCGACGGTCCGAATAGTCGGTCAAATCCCCACCTCGTCGTTGGACCGTGTCCAAGAATCTGAAGTAGTAGAAGCCGGATGCCTCCACCTAAAAACACTTATCTGTTATTTTTGGAATTAATTTTGATGGTTGCTGTCAACTCTGTCAGTTAGTGGCAAGAGGGTACCTCTGATGTGAGGGAGAGAACTGCAGAACGAACGGAAGAAAGTGTTGATGTAAGAGGTTACCACTAGTAGTACTGTTTGTCTGTATCAGGAATGTGATTATAATTTAACCTGTCCTCCAAAACCGTACGAGTCCTGCGCAAGATCTTGTTCCAATTATTGGCCAGACCTGGACAGCTTTTAGGCTGTGTTCGGCACAGCACTTTCA
CDS Sequence
  • >LOC_Os07g40290.1
    ATGGCGGTGATGACTGATGTGTCGACCACCGGGACGGCACTCCGGACCCCGGCGGCGGGGGCGGTGAAGGAGGGCGACGTCGAGAAGCTCCGGTTCATCGACGAGATGACCACCAACGTCGACGCCGTGCAGGAGCGCGTCCTGGGGGAGATCCTCGGCCGCAACGCCGGCACGGAGTACCTGACCAAGTGCGGCCTCGACGGCGCCACCGACCGCGCCGCCTTCCGGGCCAAGGTTCCGGTGGTGTCGTACGACGACCTGCAGCCGTACATCCAGCGCATTGCGAACGGGGACCGCTCGCCGATCCTGTCCACCCACCCCGTCTCCGAGTTCCTCACCAGCTCCGGCACGTCGGCCGGCGAGCGCAAGCTGATGCCCACCATCATGGACGAGCTCGACCGCCGTCAGCTGCTCTACAGCCTCCTCATGCCGGTCATGAACTTGTATGTGCCTGGGCTTGACAAGGGGAAGGGGCTCTACTTCCTGTTCGTCAAGTCGGAGACGAAGACGCCGGGAGGCCTGACGGCGAGGCCCGTGCTGACGAGCTACTACAAGAGCGACCACTTCAAGAACCGGCCGTACGACCCGTACCACAACTACACGAGCCCGACGGCGGCCATCCTCTGCGCCGACGCGTTCCAGAGCATGTACGCGCAGATGGTGTGCGGCCTGTGCCAGCGCAACGACGTGCTGCGCCTCGGCGCCGTGTTCGCCTCCGGCCTCCTCCGGGCCATCCGCTTCCTCCAGCTCAACTGGGAGCAGCTCGCCGACGACATCGAGTCCGGCGAGCTCACCCCTCGCGTCACCGACCCGTCGGTGCGCGAGGCTGTCGCCGCCATCCTCCTCCCGGACCCCGAGCTCGCCAAGCTCATCCGCGCCGAGTGCTCCAAGGGCGACTGGGCCGGCATCATCACCCGCGTGTGGCCGAACACCAAGTACCTCGACGTCATCGTCACCGGCGCGATGGCGCAGTACATCCCGACCCTGGAGTTCTACAGCGGCGGGCTTCCCATGGCGTGCACCATGTACGCGTCATCCGAGTGCTACTTCGGCCTCAACCTCCGCCCCATGTGCGACCCCTCCGAGGTGTCCTACACCATCATGCCTAACATGGGCTACTTCGAGTTCCTCCCCGTGGACGAGACCGGCGCGGCTTCGGGCGACGCCACCCAGCTCGTGGACCTCGCCCGCGTGGAGGTGGGGCGCGAGTACGAGCTGGTGATCACCACCTACGCCGGGCTGAACCGGTACCGCGTCGGCGACGTCCTCCGCGTGACGGGGTTCCACAACGCGGCGCCGCAGTTCAGGTTCGTGCGCCGCAAGAACGTGCTCCTCTCCATCGAGTCCGACAAGACCGACGAGGCCGAGCTGCAGCGCGCCGTGGAGCGCGCGTCGGCGCTGCTCCGCCCGCACGGCGCGTCCGTGGTGGAGTACACCAGCCAAGCGTGCACCAAGCGCATCCCGGGCCACTACGTCATCTACTGGGAGCTCCTGACCAAGGGCGCCGGCGCCACGGTGGTGGACGCGGACACGCTGGGCAGGTGCTGCCTCGAGATGGAGGAGGCGCTGAACACGGTGTACAGGCAGAGCCGCGTCGCGGACGGCTCGATTGGGCCGCTCGAGATCCGGGTCGTCCGCCCGGGCACATTCGAGGAGCTCATGGACTACGCCATCTCCCGCGGCGCGTCCATCAACCAGTACAAGGTGCCACGCTGCGTCACGTTCCCGCCCATCGTCGAGCTGCTCGACTCGCGCGTCGTGTCCAGCCACTTCAGCCCGGCGCTCCCACACTGGACTCCGGCGCGACGGTCCGAATAG
Protein Sequence
  • >LOC_Os07g40290.1
    MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARRSE*