Gene Details:

• MSU gene ID: LOC_Os07g30510
• RAPdb gene ID: Os07g0488400
• Gene Symbol: LIL1 ALS1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Under natural field conditions, the leaves of LIL1 mutant plants exhibited light-induced, small, rust-red lesions that first appeared at the leaf tips and subsequently expanded throughout the entire leaf blade to the leaf sheath.
• The LIL1 mutants also displayed increased expression of defense-related genes and enhanced resistance to rice blast fungus (Magnaporthe grisea).
• Thus, LIL1 is a novel LMM in rice that will facilitate the further study of the molecular mechanisms of HR and the rice blast resistance.
• Histochemical staining indicated that LIL1 lesions displayed an abnormal accumulation of reactive oxygen species (ROS) and resulted from programmed cell death (PCD).
• Sequence analysis of these 12 candidate genes revealed a G to A base substitution in the fourth exon of LOC_Os07g30510, a putative cysteine-rich receptor-like kinase (CRK), which led to an amino acid change (Val 429 to Ile) in the LIL1 protein.
• Lesion mimic mutants (LMMs), such as LIL1 that was identified in an ethylmethane sulfonate mutagenized population of Indica rice (Oryza sativa L.
• Overexpression of the LOC_Os07g30510 gene from LIL1 induced a LIL1-like lesion phenotype in Nipponbare.
• A semi-dominant lesion mimic mutant ALS1 (apoptosis leaf and sheath 1) in rice was identified to demonstrate spontaneous lesions on the leaf blade and sheath.
• ALS1 was expressed mainly in the leaf blade and sheath, in which further study revealed that ALS1 was present in the vascular bundles.
• Functional studies of ALS1 were conducted, including phylogenetic analysis, expression analysis, subcellular location and blast resistance identification.
• The mutation of ALS1 caused a constitutively activated defence response in ALS1.
• The results of our study imply that ALS1 participate in a defence response resembling the common SA-, JA- and NH1- mediated defence responses in rice.

Function-related keywords:

• leaf ,  resistance ,  sheath ,  blast ,  cell-death ,  Kinase ,  blast-resistance ,  lesion ,  reactive-oxygen-species ,  lesion-mimic ,  vascular-bundle ,  defence ,  defence-response

Literature:

• Identification and Map-Based Cloning of the Light-Induced Lesion Mimic Mutant 1 (LIL1) Gene in Rice .  DOI:  10.3389/fpls.2017.02122 ;  PMID:  29312386
• Semi-dominant mutation in the cysteine-rich receptor-like kinase gene, ALS1, conducts constitutive defence response in rice .  DOI:  10.1111/plb.12896 ;  PMID:  30101415

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: