Gene Details:
- MSU gene ID: LOC_Os07g26540
- RAPdb gene ID: Os07g0446800
- Gene Symbol: OsHXK1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Overexpression of OsHXK1 also resulted in the overaccumulation of ROS, premature initiation of PCD, and pollen abortion.
- Moreover, knockdown of OsHXK1 restored pollen fertility in OsAGO2 knockdown plants.
- OsAGO2 controls ROS production and the initiation of tapetal PCD by epigenetically regulating OsHXK1 expression in rice anthers.
- These results indicate that OsHXK1 controls the appropriate production of ROS and the proper timing of tapetal PCD and is directly regulated by OsAGO2 through epigenetic regulation.
- They also revealed the important roles of OsHXK1 in the regulation of rice yield and photosynthesis.
- Improving the Rice Photosynthetic Efficiency and Yield by Editing OsHXK1 via CRISPR/Cas9 System.
- Hexokinase gene OsHXK1 positively regulates leaf senescence in rice.
- CONCLUSIONS: Overall, the outcomes of this study suggested that OsHXK1 could act as a positive regulator of rice leaf senescence by mediating glucose accumulation and inducing an increase in ROS.
- The upregulation of OsHXK1 led to premature senescence of rice leaves, a decreased level of chlorophyll, and damage to the chloroplast structure.
- The overexpression of OsHXK1 resulted in increases in glucose and ROS levels and produced programmed cell death (PCD) signals earlier at the booting stage.
- OsHXK1 acts as a positive regulator of blast disease resistance that OsHXK1-OE rice exhibited enhanced resistance, whereas Cas9-OsHXK1 rice showed reduced resistance, against M.
- OsHXK1 may function through its sugar sensor activity since glucose induced defense-related gene expression and ROS accumulation in Nipponbare and OsHXK1-OE but not in Cas9-OsHXK1 rice.
Function-related keywords:
- pollen , fertility , tapetal , PCD , photosynthesis , yield , leaf , leaf-senescence , chloroplast , senescence , cell-death , programmed-cell-death , resistance , disease , disease-resistance , blast , sugar , blast-disease
Literature:
- OsAGO2 controls ROS production and the initiation of tapetal PCD by epigenetically regulating OsHXK1 expression in rice anthers . DOI: 10.1073/pnas.1817675116 ; PMID: 30902896
- Improving the Rice Photosynthetic Efficiency and Yield by Editing OsHXK1 via CRISPR/Cas9 System . DOI: 10.3390/ijms22179554 ; PMID: 34502462
- Hexokinase gene OsHXK1 positively regulates leaf senescence in rice . DOI: 10.1186/s12870-021-03343-5 ; PMID: 34879830
- An OsPRMT5-OsAGO2/miR1875-OsHXK1 module regulates rice immunity to blast disease . DOI: 10.1111/jipb.13430 ; PMID: 36511124
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os07g26540.1
CGGATCGCCAAGAAACCTTCGTGCGGTTCAAAGCTTGTTCGATTCGTTCGCGCGGGACCATGGCGGCGGCGGCGGTGGCGGCAGATCAGAAGGTGGTGACGATGACGAGCCTCCGGGAGGGCTGCGCTTGCGCGGCGCCTCCTGCTGCAGCTGCGCCGCCGATGCCGAAGATGGCGGCGGCGCAGAGGGTGGTGGCGGAGCTGAGAGAAGCGTGCGCGACGCCGGCGGCGAGGCTGGCGGAGGTGGCCGCGGCGATGGCCGGCGAGATGGAGGCCGGGCTGGCGGTGGAGGGCGGCAGCAGCGAGATGAAGATGATCGTGTCGTACGTCGACAGCCTCCCCACCGGCGGCGAGGAGGGGTCGTACTACGCGCTCGACCTCGGCGGCACCAACTTCCGCGTCCTCCGCGTGCGGCTTGCCGGCGGCGGCGTCGCCGAGCGCGTGGCGAGGGAGGTCCCGATCCCTCCCGGCCTCATGTCCGGCGGCGGCGCCACCTCGGAGTGCCTCTTCGGCTTCATCGCCTCCGCGCTAGCCGAGTTCGTCGGCGAGGAGGAAGAAGAAGGCGGCCTCGACGGCGGCGAGAGGGAGCTTGGGTTCACCTTCTCCTTCCCCGTGCACCAAACCTCCATCGCGTCGGGGACGCTCATCCGGTGGACGAAGGCGTTCGCCGTCGACGACGCGATCGGCGAGGACGTCGTGGCGGCGCTGCAGGCGGCCATGTCGGAGCGGGGGCTCGACATGCGCGTGTCGGCGCTCATCAACGACACCGTCGGGACGCTCGCCGCGGGCAGCTACTACGACGAGGACGTCGTGGCCGCCGTCATCCTCGGCACCGGCACGAACGCCGCCTACGTCGAGGACGCCACCGCCATCGCCAAGCTACACCCATCGCAGCTGCCAGCATCGAACACCATGGTGATCAACACCGAGTGGGGCAGCTTCGCCTCGCCGTGCCTCCCATTGACGGAGTTCGACGAAGCACTCGATCAGGAGAGCCTCAACCCCGGCGAGCAGACCTACGAGAAGCTCATCTCCGGGATGTACCTCGGCGAGATCGTCAGGAGGGTCCTCCTCAAGATCTCCTCCCGGTGCCCCTCCCTCCTCGGCGGCGCCGGCGAGCTCGCGACGCCGTTCGTCCTCAGGACACCCGACGTGTCCGCGATGCACCACGACGAGACGCCCGACCTGAGCATCGTCGGCGAGAAGCTGGAACGCACGCTGGGCATCCGCGGCACGTCGCCGGAGGCGAGGAGGATGGTCGTCGAGGTGTGCGACATCGTCGCCACGAGGGCCGCCCGGCTGGCCGCGGCGGGGATCGTCGGGATCCTGAAGAAGATCGGGAGGGTCGACGGCGGCGAGGGGCGGAGGAGGAGGTCGGTGGTCGCCGTGGACGGCGGGCTGTTCGAGCACTACGGCAAGTTCCGGCGGTGCATGGAGAGCGCGGTGAGGGAGCTGCTCGGAGAGGCGGCGGCGGAGAGGGTGGTCGTCAAGCTCGCCAGCGACGGCTCCGGGCTCGGCGCCGCCCTGGTTGCAGCTGCTCACTCGCAGAGAGCATAATATTGAATTGTGGTGAAATATGCGTGTGTTTGAAACAAGCTTGAATGATTTGAATATTTGATGCGATTTGCAAAACCACATATGAAATAGAAGC
CDS Sequence
- >LOC_Os07g26540.1
ATGGCGGCGGCGGCGGTGGCGGCAGATCAGAAGGTGGTGACGATGACGAGCCTCCGGGAGGGCTGCGCTTGCGCGGCGCCTCCTGCTGCAGCTGCGCCGCCGATGCCGAAGATGGCGGCGGCGCAGAGGGTGGTGGCGGAGCTGAGAGAAGCGTGCGCGACGCCGGCGGCGAGGCTGGCGGAGGTGGCCGCGGCGATGGCCGGCGAGATGGAGGCCGGGCTGGCGGTGGAGGGCGGCAGCAGCGAGATGAAGATGATCGTGTCGTACGTCGACAGCCTCCCCACCGGCGGCGAGGAGGGGTCGTACTACGCGCTCGACCTCGGCGGCACCAACTTCCGCGTCCTCCGCGTGCGGCTTGCCGGCGGCGGCGTCGCCGAGCGCGTGGCGAGGGAGGTCCCGATCCCTCCCGGCCTCATGTCCGGCGGCGGCGCCACCTCGGAGTGCCTCTTCGGCTTCATCGCCTCCGCGCTAGCCGAGTTCGTCGGCGAGGAGGAAGAAGAAGGCGGCCTCGACGGCGGCGAGAGGGAGCTTGGGTTCACCTTCTCCTTCCCCGTGCACCAAACCTCCATCGCGTCGGGGACGCTCATCCGGTGGACGAAGGCGTTCGCCGTCGACGACGCGATCGGCGAGGACGTCGTGGCGGCGCTGCAGGCGGCCATGTCGGAGCGGGGGCTCGACATGCGCGTGTCGGCGCTCATCAACGACACCGTCGGGACGCTCGCCGCGGGCAGCTACTACGACGAGGACGTCGTGGCCGCCGTCATCCTCGGCACCGGCACGAACGCCGCCTACGTCGAGGACGCCACCGCCATCGCCAAGCTACACCCATCGCAGCTGCCAGCATCGAACACCATGGTGATCAACACCGAGTGGGGCAGCTTCGCCTCGCCGTGCCTCCCATTGACGGAGTTCGACGAAGCACTCGATCAGGAGAGCCTCAACCCCGGCGAGCAGACCTACGAGAAGCTCATCTCCGGGATGTACCTCGGCGAGATCGTCAGGAGGGTCCTCCTCAAGATCTCCTCCCGGTGCCCCTCCCTCCTCGGCGGCGCCGGCGAGCTCGCGACGCCGTTCGTCCTCAGGACACCCGACGTGTCCGCGATGCACCACGACGAGACGCCCGACCTGAGCATCGTCGGCGAGAAGCTGGAACGCACGCTGGGCATCCGCGGCACGTCGCCGGAGGCGAGGAGGATGGTCGTCGAGGTGTGCGACATCGTCGCCACGAGGGCCGCCCGGCTGGCCGCGGCGGGGATCGTCGGGATCCTGAAGAAGATCGGGAGGGTCGACGGCGGCGAGGGGCGGAGGAGGAGGTCGGTGGTCGCCGTGGACGGCGGGCTGTTCGAGCACTACGGCAAGTTCCGGCGGTGCATGGAGAGCGCGGTGAGGGAGCTGCTCGGAGAGGCGGCGGCGGAGAGGGTGGTCGTCAAGCTCGCCAGCGACGGCTCCGGGCTCGGCGCCGCCCTGGTTGCAGCTGCTCACTCGCAGAGAGCATAA
Protein Sequence
- >LOC_Os07g26540.1
MAAAAVAADQKVVTMTSLREGCACAAPPAAAAPPMPKMAAAQRVVAELREACATPAARLAEVAAAMAGEMEAGLAVEGGSSEMKMIVSYVDSLPTGGEEGSYYALDLGGTNFRVLRVRLAGGGVAERVAREVPIPPGLMSGGGATSECLFGFIASALAEFVGEEEEEGGLDGGERELGFTFSFPVHQTSIASGTLIRWTKAFAVDDAIGEDVVAALQAAMSERGLDMRVSALINDTVGTLAAGSYYDEDVVAAVILGTGTNAAYVEDATAIAKLHPSQLPASNTMVINTEWGSFASPCLPLTEFDEALDQESLNPGEQTYEKLISGMYLGEIVRRVLLKISSRCPSLLGGAGELATPFVLRTPDVSAMHHDETPDLSIVGEKLERTLGIRGTSPEARRMVVEVCDIVATRAARLAAAGIVGILKKIGRVDGGEGRRRRSVVAVDGGLFEHYGKFRRCMESAVRELLGEAAAERVVVKLASDGSGLGAALVAAAHSQRA*