Gene Details:

Functional Descriptions:

  • The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice.
  • Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl-CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine.
  • Indeed, the OsPKS2 mutant had defective exine patterning and was male sterile.
  • OsPKS2 is required for rice male fertility by participating in pollen wall formation.
  • Results suggested that OsPKS2 is critical for pollen wall formation, and plays a conserved but differentiated role in sporopollenin biosynthesis from Arabidopsis.
  • Two other allelic mutants of OsPKS2 were generated using the CRISPR/Cas9 system and are also completely male sterile.
  • This result thus further confirmed that OsPKS2 controls rice male fertility.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os07g22850.1
    ATGGTGAGCACCAATGCCGGCGGTATTGCGAGCAAGCAGGCGTCGTCCATGGCCCCCAACCCGGGCAAGGCCACGATCCTCGCCCTCGGCCACGCCTTCCCGCAGCAGCTGGTCATGCAGGACTACGTCGTCGACGGCTTCATGAGGAACACCAACTGCGACGACCCGGAGCTCAAGGAGAAGCTCACCAGACTCTGTACCGTACCTGATCCAAATCTGATCATTTGCAGTTACAAATATATATACTCCACTATTATCGAACTTGCGTGCAAGACGACGACGGTGAAGACGAGGTACGTGGTGATGTCGGAGGAGATCCTCAAGAGCTACCCGGAGCTGGCGCAGGAGGGCCAGCCGACGATGAAGCAGCGGCTGGACATCTCCAACAAGGCGGTGACGCAGATGGCGACGGAGGCGTCGCTCGCCTGCGTCCGCTCCTGGGGCGGCGCGCTCTCGGAGATCACCCACCTTGTCTACGTCTCCTCCAGCGAGGCGCGGTTCCCCGGCGGAGACCTCCACCTGGCGCGCGCGCTGGGCCTCAGCCCGGACGTCCGCCGCGTCATGCTGGCGTTCACCGGCTGCTCGGGCGGCGTCGCGGGCCTCCGCGTCGCCAAGGGCCTCGCCGAGAGCTGCCCGGGTGCGCGCGTCCTCCTCGCCACCTCCGAGACCACCATCGTCGGGTTCCGCCCGCCCAGCCCCGACCGCCCCTACGACCTCGTCGGCGTCGCCCTCTTCGGCGACGGCGCCGGCGCGGCCGTGGTCGGCGCCGACCCGACACCGGTGGAGCGCCCGCTGTTCGAGCTCCACTCGGCGCTGCAGCGGTTCCTCCCCGACACCGACAAGACCATCGACGGGCGGCTGACGGAGGAGGGCATCAAGTTCCAGCTCGGCCGCGAACTCCCCCACATCATCGAGGCCAACGTGGAGGCCTTCTGCCAGAAGCTGATGCAGGAGCACCCTCAGGCGGCGGACAAGCTCACCTACGGCGACATGTTCTGGGCGGTGCACCCCGGCGGGCCGGCGATCCTGACCAAGATGGAGGGCAGGCTGGGGCTGGACGGCGGGAAGCTCCGCGCCAGCCGGAGCGCGCTCCGGGACTTCGGGAACGCGAGCAGCAACACCATCGTGTACGTGCTGGAGAACATGGTGGAGGAGACCCGGCAGAGGAGGGAGGAGGCGGCGGAAGAGGAGGATTGCGAGTGGGGGCTCATACTGGCGTTCGGGCCGGGGATCACGTTCGAGGGGATCCTGGCCAGAAATCTGCAGGCGCGCGCGCGCGCGCGCGACTGAGGAGGGAGGGAGACAGGGATCGGACTCTGATTGATCACGATGTATCGGGCCTCAGCGCAATGCAAGAAACATTTTCGCTGTCCAGACTAATAAAAGGCGAAAGTGTTTTCTTCCCTATAAAAGATATCCTCTCGTTGCGTGCAATGTTATCTAAATAGTTTAAAAAAATTAAAAAAAATTGAAAAGATAGATTAATATGAAA
CDS Sequence
  • >LOC_Os07g22850.1
    ATGGTGAGCACCAATGCCGGCGGTATTGCGAGCAAGCAGGCGTCGTCCATGGCCCCCAACCCGGGCAAGGCCACGATCCTCGCCCTCGGCCACGCCTTCCCGCAGCAGCTGGTCATGCAGGACTACGTCGTCGACGGCTTCATGAGGAACACCAACTGCGACGACCCGGAGCTCAAGGAGAAGCTCACCAGACTCTGTACCGTACCTGATCCAAATCTGATCATTTGCAGTTACAAATATATATACTCCACTATTATCGAACTTGCGTGCAAGACGACGACGGTGAAGACGAGGTACGTGGTGATGTCGGAGGAGATCCTCAAGAGCTACCCGGAGCTGGCGCAGGAGGGCCAGCCGACGATGAAGCAGCGGCTGGACATCTCCAACAAGGCGGTGACGCAGATGGCGACGGAGGCGTCGCTCGCCTGCGTCCGCTCCTGGGGCGGCGCGCTCTCGGAGATCACCCACCTTGTCTACGTCTCCTCCAGCGAGGCGCGGTTCCCCGGCGGAGACCTCCACCTGGCGCGCGCGCTGGGCCTCAGCCCGGACGTCCGCCGCGTCATGCTGGCGTTCACCGGCTGCTCGGGCGGCGTCGCGGGCCTCCGCGTCGCCAAGGGCCTCGCCGAGAGCTGCCCGGGTGCGCGCGTCCTCCTCGCCACCTCCGAGACCACCATCGTCGGGTTCCGCCCGCCCAGCCCCGACCGCCCCTACGACCTCGTCGGCGTCGCCCTCTTCGGCGACGGCGCCGGCGCGGCCGTGGTCGGCGCCGACCCGACACCGGTGGAGCGCCCGCTGTTCGAGCTCCACTCGGCGCTGCAGCGGTTCCTCCCCGACACCGACAAGACCATCGACGGGCGGCTGACGGAGGAGGGCATCAAGTTCCAGCTCGGCCGCGAACTCCCCCACATCATCGAGGCCAACGTGGAGGCCTTCTGCCAGAAGCTGATGCAGGAGCACCCTCAGGCGGCGGACAAGCTCACCTACGGCGACATGTTCTGGGCGGTGCACCCCGGCGGGCCGGCGATCCTGACCAAGATGGAGGGCAGGCTGGGGCTGGACGGCGGGAAGCTCCGCGCCAGCCGGAGCGCGCTCCGGGACTTCGGGAACGCGAGCAGCAACACCATCGTGTACGTGCTGGAGAACATGGTGGAGGAGACCCGGCAGAGGAGGGAGGAGGCGGCGGAAGAGGAGGATTGCGAGTGGGGGCTCATACTGGCGTTCGGGCCGGGGATCACGTTCGAGGGGATCCTGGCCAGAAATCTGCAGGCGCGCGCGCGCGCGCGCGACTGA
Protein Sequence
  • >LOC_Os07g22850.1
    MVSTNAGGIASKQASSMAPNPGKATILALGHAFPQQLVMQDYVVDGFMRNTNCDDPELKEKLTRLCTVPDPNLIICSYKYIYSTIIELACKTTTVKTRYVVMSEEILKSYPELAQEGQPTMKQRLDISNKAVTQMATEASLACVRSWGGALSEITHLVYVSSSEARFPGGDLHLARALGLSPDVRRVMLAFTGCSGGVAGLRVAKGLAESCPGARVLLATSETTIVGFRPPSPDRPYDLVGVALFGDGAGAAVVGADPTPVERPLFELHSALQRFLPDTDKTIDGRLTEEGIKFQLGRELPHIIEANVEAFCQKLMQEHPQAADKLTYGDMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEETRQRREEAAEEEDCEWGLILAFGPGITFEGILARNLQARARARD*