Information report for OsNRAMP1
Gene Details
|
|
Functional Descriptions
- Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice.
- The growth of yeast expressing OsNRAMP1 was impaired in the presence of Cd compared with yeast transformed with an empty vector.
- The role of the iron transporter OsNRAMP1 in Cd uptake and transport in rice was investigated here.
- The OsNRAMP1 iron transporter is involved in Cd accumulation in rice.
- The expression of OsNRAMP1 in the roots was higher in a high Cd-accumulating cultivar (Habataki) than a low Cd-accumulating cultivar (Sasanishiki) regardless of the presence of Cd, and the amino acid sequence of OsNRAMP1 showed 100% identity between Sasanishiki and Habataki.
- These results suggest that OsNRAMP1 participates in cellular Cd uptake and Cd transport within plants, and the higher expression of OsNRAMP1 in the roots could lead to an increase in Cd accumulation in the shoots.
- Rice has seven putative metal transporter NRAMP genes, but microarray analysis showed that only OsNRAMP1 is highly up-regulated by iron (Fe) deficiency.
- OsNRAMP1 expression was observed mainly in roots and was higher in the roots of a high-Cd-accumulating cultivar (Habataki) than in those of a low-Cd-accumulating cultivar (Sasanishiki).
- These results suggest that OsNRAMP1 participates in cellular Cd uptake and that the differences observed in Cd accumulation among cultivars are because of differences in OsNRAMP1 expression levels in roots.
- Immunostaining showed that OsNRAMP1 was localized in all root cells except the central vasculature, and in leaf mesophyll cells.
- The knockout of OsNRAMP1 resulted in significant decreases in root uptake of Cd and Mn and their accumulation in rice shoots and grains, and increased sensitivity to Mn deficiency.
- OsNRAMP1 contributes to cadmium and manganese uptake in rice.
- Here, we showed that OsNRAMP1 was able to transport Cd and manganese (Mn) when expressed in yeast, but did not transport Fe or As.
- OsNRAMP1 was mainly expressed in roots and leaves and encoded a plasma membrane-localized protein.
- Previous studies reported that the rice OsNRAMP1 (Natural Resistance-Associated Macrophage Protein 1) could transport iron (Fe), Cd and arsenic (As) in heterologous yeast assays.
- OsNRAMP1 expression was induced by Cd treatment and Fe deficiency.
- The rice heavy metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homeostasis.
- The OsNRAMP1 mutants had increased content of H(2) O(2) and activity of superoxide dismutase, but decreased activity of catalase, exhibiting enhanced broad-spectrum resistance against bacterial and fungal pathogens.
- Altered expression of metal ion-dependent ROS-scavenging enzymes genes and lower accumulation of cations such as Mn coordinately triggered compromised metal ion-dependent enzyme-catalyzing activity and modulated ROS homeostasis, which together contributed to disease resistance in OsNRAMP1 mutants.
- Taken together, a proof-of-concept was achieved that broad-spectrum disease resistance and toxic heavy metal-safe rice was engineered by removal of OsNRAMP1 gene.
- Here, we show that rice heavy metal transporter OsNRAMP1 plays important roles in plant immunity by modulating metal ions and reactive oxygen species (ROS) homeostasis.
Functional Keywords
- cadmium , growth , iron , transporter , root , shoot , leaf , manganese , plasma-membrane , Fe , resistance , disease , disease-resistance , immunity , homeostasis , metal-transport , broad-spectrum-disease-resistance , reactive-oxygen-species
Literature and News
- The OsNRAMP1 iron transporter is involved in Cd accumulation in rice . DOI: 10.1093/jxb/err136 ; PMID: 21697258
- Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice . DOI: 10.4161/psb.6.11.17587 ; PMID: 22067109
- OsNRAMP1 transporter contributes to cadmium and manganese uptake in rice . DOI: 10.1111/pce.13843 ; PMID: 32666540
- Calcium Plays a Double-Edged Role in Modulating Cadmium Uptake and Translocation in Rice . DOI: 10.3390/ijms21218058 ; PMID: 33137932
- Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency . DOI: 10.1111/plb.13227 ; PMID: 33296551
- Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment . DOI: 10.1371/journal.pone.0243174 ; PMID: 33301482
- Cadmium Inhibits Lateral Root Emergence in Rice by Disrupting OsPIN-Mediated Auxin Distribution and the Protective Effect of OsHMA3 . DOI: 10.1093/pcp/pcaa150 ; PMID: 33300991
- The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis . DOI: 10.1111/pce.14263 ; PMID: 35040151
- Melatonin reduces cadmium accumulation via mediating the nitric oxide accumulation and increasing the cell wall fixation capacity of cadmium in rice . DOI: 10.1016/j.jhazmat.2022.130529 ; PMID: 37055957
Gene Resources
- UniProt: A0A1Y1B854
- EMBL: LC196121
- AlphaFoldDB: A0A1Y1B854
- EnsemblPlants: Os07t0258400-01, Os07t0258400-03
- Gramene: Os07t0258400-01, Os07t0258400-03
- KEGG: dosa:Os07g0258400
- Orthologous matrix: GMKDACR
- InterPro: IPR001046
- PANTHER: PTHR11706, PTHR11706:SF41
- OrthoDB: A0A1Y1B854
- SWISS-MODEL: A0A1Y1B854
Sequences
cDNA Sequence
- >LOC_Os07g15460.1
TTGCAGAGATCGGGGTTCGGCGCCGGCGGCGAGTTGTTGAGAGGCAAGGCAAACTCAGGCGACTGAGCTAAAGAAGCCGCACTAGTATGGGGGTGACGAAGGCGGAGGCGGTTGCCGGCGACGGCGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAGGTTTTTGTCCCACATTGGACCAGGCTTTATGGTCTGTTTGGCTTACCTCGATCCTGGAAATATGGAGACTGATCTACAAGCTGGTGCCAATCACAAATATGAGCTTCTTTGGGTGATTTTGATTGGCCTGATCTTTGCACTGATTATACAATCATTATCGGCTAATCTTGGAGTGGTGACAGGGCGCCATCTTGCTGAGCTGTGCAAGACTGAATATCCAGTATGGGTGAAAACCTGCTTATGGCTGCTAGCAGAACTAGCTGTGATTGCTTCCGATATTCCAGAAGTTATAGGTACAGGATTTGCTTTCAACCTTTTGTTCCACATACCAGTGTGGACCGGGGTTCTCATTGCTGGCTCTAGCACGCTTCTTCTTCTTGGACTGCAAAGATATGGGGTAAGGAAACTGGAGGTTGTGGTCGCGCTATTGGTGTTTGTCATGGCAGGGTGCTTCTTCGTGGAGATGAGCATAGTCAAGCCTCCGGTTAATGAGGTCCTTCAAGGATTATTCATCCCCAGGCTCAGTGGGCCCGGTGCCACAGGAGACTCCATTGCCCTTCTTGGGGCTCTTGTAATGCCGCACAATCTATTCTTGCACTCTGCCCTGGTGCTGTCGAGGAATACACCCGCATCAGCAAAAGGAATGAAGGATGTCTGTAGGTTCTTCCTCTTTGAGAGTGGGATAGCTCTTTTCGTGGCTCTGCTTGTCAACATTGCAATCATCTCTGTCTCCGGCACTGTATGTAATGCAACCAACCTTTCACCAGAAGATGCTGTAAAATGCAGCGACCTTACATTGGACTCCTCATCCTTCCTCCTCAGGAATGTGCTAGGAAAGTCAAGTGCGACAGTGTACGGTGTAGCACTCTTGGCTTCTGGACAGAGCTCGACCATTACTGGCACTTATGCTGGGCAATATGTTATGCAGGGGTTCCTAGACATCAAAATGAAACAGTGGCTTCGGAACCTGATGACACGCAGCATTGCCATTGTGCCTAGCTTAATCGTCTCCATCATTGGAGGGTCTAGTGGCGCCGGTCGTCTCATCGTCATTGCATCGATGATTCTATCCTTTGAGCTACCATTTGCTCTGATCCCTCTTCTCAAGTTCAGCAGCAGCAGCAACAAGATGGGTGAAAACAAGAACTCCATCTATATTGTTGGATTCTCCTGGGTGCTGGGGTTCGTCATCATCGGGATAAACATCTACTTCCTCAGCACAAAGCTGGTCGGCTGGATCCTCCACAACGCGCTCCCGACATTCGCCAACGTGCTCATCGGCATCGTGCTGTTCCCGCTCATGCTGCTCTACGTCGTCGCCGTGATCTACCTGACGTTCAGGAAGGACACCGTCAAGTTCGTGTCTCGTCGTGAGCTGCAGGCCGGCGACGACACCGAGAAAGCACAGGTAGCCACCTGCGTCGCCGACGAGCACAGCAAAGAGCCACCCGTGTAGTACCCTTCCAGACCGGTTACCGGTGTTGGCTGGTTTTTATTGGATAGATGTTTAGGTCCAATTTTTTTTATATAAAAAATCCAATAGATGTTTAGGTCCAAATTTTTGAATTCAAAAACCGGTTAGCAGTGATAACCGGCTGAAATTTTGGCCCATGGGAGTAGATTGTTGCTTTTTTTCTTAGTGATTAGTTATTGGCAGATTGGCAGAATGTTGTAGCTAGGCAATCTAATTGTATGGTTAGATATTTAGTCATAAATCCAAGAGTGAGTTTTCTTGGATGCGAGGACAAGGACAATCTAATTGTATGGTTAGATATTTAGTCATAAATCCAAGAGTGAGTTTTCTTGGATGCGAGGACAAGGACAAATATAAATGTAAATAAATGCAGTTAAAGTAACACCTTGCTCAAATATAAATGTAAATAAATGCAGTTAAAGTAACACCTTGCTCCTTGTGGACATCTA - >LOC_Os07g15460.2
TTGCAGAGATCGGGGTTCGGCGCCGGCGGCGAGTTGTTGAGAGGCAAGGCAAACTCAGGCGACTGAGCTAAAGAAGCCGCACTAGTATGGGGGTGACGAAGGCGGAGGCGGTTGCCGGCGACGGCGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAGGTTTTTGTCCCACATTGGACCAGGCTTTATGGTCTGTTTGGCTTACCTCGATCCTGGAAATATGGAGACTGATCTACAAGCTGGTGCCAATCACAAATATGAGCTTCTTTGGGTGATTTTGATTGGCCTGATCTTTGCACTGATTATACAATCATTATCGGCTAATCTTGGAGTGGTGACAGGGCGCCATCTTGCTGAGCTGTGCAAGACTGAATATCCAGTATGGGTGAAAACCTGCTTATGGCTGCTAGCAGAACTAGCTGTGATTGCTTCCGATATTCCAGAAGTTATAGGTACAGGATTTGCTTTCAACCTTTTGTTCCACATACCAGTGTGGACCGGGGTTCTCATTGCTGGCTCTAGCACGCTTCTTCTTCTTGGACTGCAAAGATATGGGGTAAGGAAACTGGAGGTTGTGGTCGCGCTATTGGTGTTTGTCATGGCAGGGTGCTTCTTCGTGGAGATGAGCATAGTCAAGCCTCCGGTTAATGAGGTCCTTCAAGGATTATTCATCCCCAGGCTCAGTGGGCCCGGTGCCACAGGAGACTCCATTGCCCTTCTTGGGGCTCTTGTAATGCCGCACAATCTATTCTTGCACTCTGCCCTGGTGCTGTCGAGGAATACACCCGCATCAGCAAAAGGAATGAAGGATGTCTGTAGGTTCTTCCTCTTTGAGAGTGGGATAGCTCTTTTCGTGGCTCTGCTTGTCAACATTGCAATCATCTCTGTCTCCGGCACTGTATGTAATGCAACCAACCTTTCACCAGAAGATGCTGTAAAATGCAGCGACCTTACATTGGACTCCTCATCCTTCCTCCTCAGGAATGTGCTAGGAAAGTCAAGTGCGACAGTGTACGGTGTAGCACTCTTGGCTTCTGGACAGAGCTCGACCATTACTGGCACTTATGCTGGGCAATATGTTATGCAGGGGTTCCTAGACATCAAAATGAAACAGTGGCTTCGGAACCTGATGACACGCAGCATTGCCATTGTGCCTAGCTTAATCGTCTCCATCATTGGAGGGTCTAGTGGCGCCGGTCGTCTCATCGTCATTGCATCGATGATTCTATCCTTTGAGCTACCATTTGCTCTGATCCCTCTTCTCAAGTTCAGCAGCAGCAGCAACAAGATGGGTGAAAACAAGAACTCCATCTATATTGTTGGATTCTCCTGGGTGCTGGGGTTCGTCATCATCGGGATAAACATCTACTTCCTCAGCACAAAGCTGGTCGGCTGGATCCTCCACAACGCGCTCCCGACATTCGCCAACGTGCTCATCGGCATCGTGCTGTTCCCGCTCATGCTGCTCTACGTCGTCGCCGTGATCTACCTGACGTTCAGGAAGGACACCGTCAAGTTCGTGTCTCGTCGTGAGCTGCAGGCCGGCGACGACACCGAGAAAGCACAGGTAGCCACCTGCGTCGCCGACGAGCACAGCAAAGAGCCACCCGTGTAGTACCCTTCCAGACCGGTTACCGGTGTTGGCTGGTTTTTATTGGATAGATGTTTAGGTCCAATTTTTTTTATATAAAAAATCCAATAGATGTTTAGGTCCAAATTTTTGAATTCAAAAACCGGTTAGCAGTGATAACCGGCTGAAATTTTGGCCCATGGGAGTAGATTGTTGCTTTTTTTCTTAGTGATTAGTTATTGGCAGATTGGCAGAATGTTGTAGCTAGGCAATCTAATTGTATGGTTAGATATTTAGTCATAAATCCAAGAGTGAGTTTTCTTGGATGCGAGGACAAGGACAATCTAATTGTATGGTTAGATATTTAGTCATAAATCCAAGAGTGAGTTTTCTTGGATGCGAGGACAAGGACAAATATAAATGTAAATAAATGCAGTTAAAGTAACACCTTGCTCAAATATAAATGTAAATAAATGCAGTTAAAGTAACACCTTGCTCCTTGTGGACATCTA
CDS Sequence
- >LOC_Os07g15460.1
ATGGGGGTGACGAAGGCGGAGGCGGTTGCCGGCGACGGCGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAGGTTTTTGTCCCACATTGGACCAGGCTTTATGGTCTGTTTGGCTTACCTCGATCCTGGAAATATGGAGACTGATCTACAAGCTGGTGCCAATCACAAATATGAGCTTCTTTGGGTGATTTTGATTGGCCTGATCTTTGCACTGATTATACAATCATTATCGGCTAATCTTGGAGTGGTGACAGGGCGCCATCTTGCTGAGCTGTGCAAGACTGAATATCCAGTATGGGTGAAAACCTGCTTATGGCTGCTAGCAGAACTAGCTGTGATTGCTTCCGATATTCCAGAAGTTATAGGTACAGGATTTGCTTTCAACCTTTTGTTCCACATACCAGTGTGGACCGGGGTTCTCATTGCTGGCTCTAGCACGCTTCTTCTTCTTGGACTGCAAAGATATGGGGTAAGGAAACTGGAGGTTGTGGTCGCGCTATTGGTGTTTGTCATGGCAGGGTGCTTCTTCGTGGAGATGAGCATAGTCAAGCCTCCGGTTAATGAGGTCCTTCAAGGATTATTCATCCCCAGGCTCAGTGGGCCCGGTGCCACAGGAGACTCCATTGCCCTTCTTGGGGCTCTTGTAATGCCGCACAATCTATTCTTGCACTCTGCCCTGGTGCTGTCGAGGAATACACCCGCATCAGCAAAAGGAATGAAGGATGTCTGTAGGTTCTTCCTCTTTGAGAGTGGGATAGCTCTTTTCGTGGCTCTGCTTGTCAACATTGCAATCATCTCTGTCTCCGGCACTGTATGTAATGCAACCAACCTTTCACCAGAAGATGCTGTAAAATGCAGCGACCTTACATTGGACTCCTCATCCTTCCTCCTCAGGAATGTGCTAGGAAAGTCAAGTGCGACAGTGTACGGTGTAGCACTCTTGGCTTCTGGACAGAGCTCGACCATTACTGGCACTTATGCTGGGCAATATGTTATGCAGGGGTTCCTAGACATCAAAATGAAACAGTGGCTTCGGAACCTGATGACACGCAGCATTGCCATTGTGCCTAGCTTAATCGTCTCCATCATTGGAGGGTCTAGTGGCGCCGGTCGTCTCATCGTCATTGCATCGATGATTCTATCCTTTGAGCTACCATTTGCTCTGATCCCTCTTCTCAAGTTCAGCAGCAGCAGCAACAAGATGGGTGAAAACAAGAACTCCATCTATATTGTTGGATTCTCCTGGGTGCTGGGGTTCGTCATCATCGGGATAAACATCTACTTCCTCAGCACAAAGCTGGTCGGCTGGATCCTCCACAACGCGCTCCCGACATTCGCCAACGTGCTCATCGGCATCGTGCTGTTCCCGCTCATGCTGCTCTACGTCGTCGCCGTGATCTACCTGACGTTCAGGAAGGACACCGTCAAGTTCGTGTCTCGTCGTGAGCTGCAGGCCGGCGACGACACCGAGAAAGCACAGGTAGCCACCTGCGTCGCCGACGAGCACAGCAAAGAGCCACCCGTGTAG - >LOC_Os07g15460.2
ATGGGGGTGACGAAGGCGGAGGCGGTTGCCGGCGACGGCGGGAAGGTGGTGGACGACATCGAGGCGCTCGCCGATCTCAGAAAGGAGCCAGCATGGAAAAGGTTTTTGTCCCACATTGGACCAGGCTTTATGGTCTGTTTGGCTTACCTCGATCCTGGAAATATGGAGACTGATCTACAAGCTGGTGCCAATCACAAATATGAGCTTCTTTGGGTGATTTTGATTGGCCTGATCTTTGCACTGATTATACAATCATTATCGGCTAATCTTGGAGTGGTGACAGGGCGCCATCTTGCTGAGCTGTGCAAGACTGAATATCCAGTATGGGTGAAAACCTGCTTATGGCTGCTAGCAGAACTAGCTGTGATTGCTTCCGATATTCCAGAAGTTATAGGTACAGGATTTGCTTTCAACCTTTTGTTCCACATACCAGTGTGGACCGGGGTTCTCATTGCTGGCTCTAGCACGCTTCTTCTTCTTGGACTGCAAAGATATGGGGTAAGGAAACTGGAGGTTGTGGTCGCGCTATTGGTGTTTGTCATGGCAGGGTGCTTCTTCGTGGAGATGAGCATAGTCAAGCCTCCGGTTAATGAGGTCCTTCAAGGATTATTCATCCCCAGGCTCAGTGGGCCCGGTGCCACAGGAGACTCCATTGCCCTTCTTGGGGCTCTTGTAATGCCGCACAATCTATTCTTGCACTCTGCCCTGGTGCTGTCGAGGAATACACCCGCATCAGCAAAAGGAATGAAGGATGTCTGTAGGTTCTTCCTCTTTGAGAGTGGGATAGCTCTTTTCGTGGCTCTGCTTGTCAACATTGCAATCATCTCTGTCTCCGGCACTGTATGTAATGCAACCAACCTTTCACCAGAAGATGCTGTAAAATGCAGCGACCTTACATTGGACTCCTCATCCTTCCTCCTCAGGAATGTGCTAGGAAAGTCAAGTGCGACAGTGTACGGTGTAGCACTCTTGGCTTCTGGACAGAGCTCGACCATTACTGGCACTTATGCTGGGCAATATGTTATGCAGGGGTTCCTAGACATCAAAATGAAACAGTGGCTTCGGAACCTGATGACACGCAGCATTGCCATTGTGCCTAGCTTAATCGTCTCCATCATTGGAGGGTCTAGTGGCGCCGGTCGTCTCATCGTCATTGCATCGATGATTCTATCCTTTGAGCTACCATTTGCTCTGATCCCTCTTCTCAAGTTCAGCAGCAGCAGCAACAAGATGGGTGAAAACAAGAACTCCATCTATATTGTTGGATTCTCCTGGGTGCTGGGGTTCGTCATCATCGGGATAAACATCTACTTCCTCAGCACAAAGCTGGTCGGCTGGATCCTCCACAACGCGCTCCCGACATTCGCCAACGTGCTCATCGGCATCGTGCTGTTCCCGCTCATGCTGCTCTACGTCGTCGCCGTGATCTACCTGACGTTCAGGAAGGACACCGTCAAGTTCGTGTCTCGTCGTGAGCTGCAGGCCGGCGACGACACCGAGAAAGCACAGGTAGCCACCTGCGTCGCCGACGAGCACAGCAAAGAGCCACCCGTGTAG
Protein Sequence
- >LOC_Os07g15460.1
MGVTKAEAVAGDGGKVVDDIEALADLRKEPAWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLSANLGVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWTGVLIAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPRLSGPGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVALLVNIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASGQSSTITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIASMILSFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWILHNALPTFANVLIGIVLFPLMLLYVVAVIYLTFRKDTVKFVSRRELQAGDDTEKAQVATCVADEHSKEPPV* - >LOC_Os07g15460.2
MGVTKAEAVAGDGGKVVDDIEALADLRKEPAWKRFLSHIGPGFMVCLAYLDPGNMETDLQAGANHKYELLWVILIGLIFALIIQSLSANLGVVTGRHLAELCKTEYPVWVKTCLWLLAELAVIASDIPEVIGTGFAFNLLFHIPVWTGVLIAGSSTLLLLGLQRYGVRKLEVVVALLVFVMAGCFFVEMSIVKPPVNEVLQGLFIPRLSGPGATGDSIALLGALVMPHNLFLHSALVLSRNTPASAKGMKDVCRFFLFESGIALFVALLVNIAIISVSGTVCNATNLSPEDAVKCSDLTLDSSSFLLRNVLGKSSATVYGVALLASGQSSTITGTYAGQYVMQGFLDIKMKQWLRNLMTRSIAIVPSLIVSIIGGSSGAGRLIVIASMILSFELPFALIPLLKFSSSSNKMGENKNSIYIVGFSWVLGFVIIGINIYFLSTKLVGWILHNALPTFANVLIGIVLFPLMLLYVVAVIYLTFRKDTVKFVSRRELQAGDDTEKAQVATCVADEHSKEPPV*