Information report for spl5;SF3b3
Gene Details
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Functional Descriptions
- ) spotted leaf 5 (spl5) indicates that wild-type spl5 negatively regulates cell death and resistance responses.
- Bioinformatic analysis showed that SPL5 gene encodes a putative splicing factor 3b subunit 3 (SF3b3) and might be involved in splicing reactions of pre-mature RNAs participating in the regulation of cell death and resistance responses.
- The data presented here clearly indicate that the spl5 negatively regulates cell death and resistance responses via modulating RNA splicing in plants.
- SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice.
- Rice mutant, spl5 (spotted leaf 5), has spontaneous hypersensitive-like lesions on its leaves and shows enhanced resistance to pathogens, indicating that spl5 plays a role in programmed cell death (PCD) and disease resistance.
- Since the serotonin plays a critical role in inducing disease-resistance, the increased serotonin level may contribute, at least partly, to the disease resistance in spl5.
- The spl5 gene may act as a negative regulatory factor activating the serotonin metabolic pathway, and these results might provide a new insight into the spl5-induced defense response mechanisms in plants.
- Interestingly, according to our microarray and real-time PCR assays, the expressions of a transcription factor OsWRKY14 and genes responsible for the biosynthesis of serotonin, anthranilate synthase (AS), indole-3-glycerolphosphate synthase (IGPS), tryptophan synthase (TS) and tryptophan decarboxylase (TDC) were significantly up-regulated in the spl5 mutant.
- Transcriptome profiling of the spl5 mutant reveals that spl5 has a negative role in the biosynthesis of serotonin for rice disease resistance.
- A 1.84-Mb region on rice chromosome 2 carrying SPL4, spl5 and MLO8 genes is associated with higher yield under phosphorus-deficient acidic soil.
Functional Keywords
Literature and News
- SPL5, a cell death and defense-related gene, encodes a putative splicing factor 3b subunit 3 (SF3B3) in rice . DOI: 10.1007/s11032-011-9677-4
- Transcriptome profiling of the spl5 mutant reveals that SPL5 has a negative role in the biosynthesis of serotonin for rice disease resistance . DOI: 10.1186/s12284-015-0052-7 ; PMID: 26029330
- A 1.84-Mb region on rice chromosome 2 carrying SPL4, SPL5 and MLO8 genes is associated with higher yield under phosphorus-deficient acidic soil . DOI: 10.1007/s13353-020-00601-4 ; PMID: 33409935
Gene Resources
- NCBI ID: P0431A02.16
Sequences
cDNA Sequence
- >LOC_Os07g10390.1
CAAAGCTGTAGTGAAATCAAGGATTGTTTGCTCATGGAACTGGGAGCCAAATGCCATGCAAGGACATCCAAGGCTGATCTTTTGCTTGGATGATGGAGAATTTCATCTTCTAGAGTTTAGTTTGGATATGGAAGGAGTGAAGGTATTGCCTGAGTGTGTCCATAGAGGTTTGCCTTGCAAACCTCTTTTGTGGATGGACAAAGGAATGGTAGTTGGATTTGTAGAGATGGGAGATGGGATGATCCTCCAACTTGAAAATAATAGATTGGTGCATAAAAGTGCAATTCAGAATGTAGCGCCAATATTGGATCTAGCAATTGCTGACCACCATGGTGAGAAGCAGGATCAGATGTTTGCATGTTGTGGAATGTGCCCTGAGGGGTCCTTGCGAGTAATACGTAATGGTGTCAATGTGGAGAAACTTCTGAGGACAGATCCTATTTATCATGGTGTTACTGGTTTGTGGACCTTGAGAATGAAGAGAACTGATGCTTATCACTCTTTTCTTGTATTGTCATTCGTTGAGGAAACCAGAATACTATCAGTAGGACTCAGTTTTAATGACATCTGTGATGCTGTAGGATTTCAGACTGATGTTTGCACTCTGGCATGTGGTTTAGTAGCTGATGGTTTGCTTGTGCAAATTCACAGCAAATGTGTAAAGCTTTGTTTACCTACAGCATGTGCTCACCCTGAGGGAACTTTGCTCCCGTCTCCTGTTTGTGCTGACTGGTATCCTGATGTCACTATCAGTGTTGGTGCTGTTGGACATAATGTTGTTGTTGTTGCTACATCGAATCCTTGTTGCCTTTACATACTTGGGGTCAGATCATTATCTTCGTTTCAGTATGAGCTGTATGAGATACAACATGTCCAGTTGCATTATGAAGTATCTTGCATATCTATTCCACAAGAGGACTGGAGACTTGATAATTCATCTTCTAGTTGTGCAACGAGTGGTGATTTTCGGAAAGATTTTGCAGCTAATATTCGCAAGTTTGCTGTAATTGGAACTCACGAACCTTCTGTGCATATCATTTCGTTGGAACCTGGAGAAGCATTTCAGCAACTTGCTGTTGGGCATATATCAGTAAACAACGCGCTCGGCACTCCTATCAGTGGTTGTATACCTGAAAATGTTAGGTTTGTAGCAGCTGCAAGGTTCTACATTCTTGCAGGACTAAGGAATGGAATGCTACTGAGGTTTGAGTCACAAACAAGCAAAGGGCATTGTTTCCCTGGTTCCTTTTACAAGGAGTCCTCTACCCCTTGTGATGATACATCCCTTATGTTGATTGCTGTACGGCGTATTGGAATCACTCCTGTAGTTTTGGTACCGTTGCATGACAGAGCCAATGCTGACATTATTGTTCTCGGTGATAGGCCTTGGCTATTGCAATCTGCTAGACACAGCTTGGCATATTCATCCATCTCATTTCTGCCTGCTTCCCATGTGACACCAGTGTCTTCTACTGATTGCCCCAGTGGTCTGCTGTTTGTTTCGGAGAACTGTTTGCATTTGGTTGAAATGGTTCATGGCAAAAGACTAAATGCACAGAAGTTTTCGATTGGAGGGACTCCAAGGAAAGTGTTATACCACAGTGATAGTAGAACACTGTTGGTGCTGAGAACTGGGTTAACCAGTGTATCCTGTTCTTCAGATATTGTTCAAATAGATCCAAGTAATGGGGCATTGCTTTCCAGATTCAAGTGCGAACCTGGTGAAACAGCAAAATGCATGCAAATTGCAAAGATAGGGAATGATCAAGTATTAATCGTTGGAACTAGTAAGTCCAATGGCCGACCAATGATGCCAAACGGTGAAGCAGAAAGTATCAAGGGTCGTCTAATTCTTTTAAGCTTGGAGACTATTGAAAGTCCTCGTGAGAGCGGTTCGTTTACTGCAGCTTCTAATTTGAATTCATCTCATGCTGGTTCTCCATTCCCGGAATTTGTTGGATATGCAGCAGAGGAGCTGTCTAGTAACAGCATGTGCAGCAGTCCTGATGAAGTTTGCTGCAATCAAATTCAACCTGAACTCATGGCAGGCCATTTGAGATCACTAGTCCAACATACATTCAATGGCGCTGTTCTTGCTGTACATCCATATCTAGATAGATATGTGTTGGCAGCAGCTGGAAATGTGCTTTTTGTATTTGGATTTTTAAATGAAAGTCCCCATCGGATAAAAAAGTATACTACGAGTAGGACACGATTTACAATAACCTGTTTGAAGACGTATGCATCACGGATTGCAGTGGGTGATTGCCGCGATGGTGTTCTCTTCTATTCTTATCATGAGAATCTTAGGAAGTTGGAACTGATCTATTCTGATCCTGCCCAACGGCTAGTGGGTGATGTTGCCCTTTTGAGCTGTGAAACTGCTGTTGTATCAGATCGACGAGGGAGCATATCTGTACTGTCTTGCCCAAGATTAGAAGTATCCGAAAGTCCAGAGAAGAACCTAGCGGTGCATTGTTCATTTTACATGGGTGAAACAGCTATGAGCATCCAGAAGGTTGCATTTAAGCATTGGCTTCCAATTGATGATTTGACAGAACCAGTGTTGGAGTCGGTATACAACTGTGTCGTCGCAAGCACTCTGCTGGGGAGTATCTTTGTTATGATTCCACTCACAAGTGAGGAGCATCAAATGTTGCAAGATGTCCAAGAAAGACTTTCGGTTCACCCATTGACTGCTCCACTCTTAGGAAATGATCATGCAGAGTTTCGTCGCCGTGGTATCCCGTCGGGAGTACCGCCCATTCTGGATGGCGACATGCTTGTGCAGTTCCTGGAGCTCACCAGCGAACAACAGCACGATGTTCTCAATATTGTTTCTCCTGGAAAGAAGAGGCAACACGATATCTCGGTTTTCCAGGTCATGCGAGCATTAGAGAGGGTGCATTACGCACTCAACTGAGGCGTTCAACAATCCATTCAACCCGGTCACTGTTCTTACCGACATGGCTGTCTCCAACCTGCTGATATTTGGGTGGCATCATCGCTGAATTTGGATCCGAGCCGAGCACCGAGTTGTTGCAGCTGAAGCTGCTACTGGAGCACATACCCAGAACATACCAAATCTCAGCTGTAGTGAGGTGCTGTGTTTTGCTGTGGGATGATAGGCCCATCTGATGTCGCAATAGTGTGTCATTGCTCTAGTGCAATCTTTGTAAATAACGAATAATATTTTGGCAGAAGGATGTGGATACAAATCCTTTTGCCCAGTGAAAGGAACAACCCTCGTGAACGCATAATCTCCATTGTGGTATATGTCAACTTGCTTCAGGTTATGCCAGTTGAAATAGGGATCTGGAAACATTTCCTGAAATCTGACGCTTGCTTGATCTTTGTGCCTTAATAAATTTGCTCTCTCCAACCATAGATTTATTTTCTTC
CDS Sequence
- >LOC_Os07g10390.1
ATGCAAGGACATCCAAGGCTGATCTTTTGCTTGGATGATGGAGAATTTCATCTTCTAGAGTTTAGTTTGGATATGGAAGGAGTGAAGGTATTGCCTGAGTGTGTCCATAGAGGTTTGCCTTGCAAACCTCTTTTGTGGATGGACAAAGGAATGGTAGTTGGATTTGTAGAGATGGGAGATGGGATGATCCTCCAACTTGAAAATAATAGATTGGTGCATAAAAGTGCAATTCAGAATGTAGCGCCAATATTGGATCTAGCAATTGCTGACCACCATGGTGAGAAGCAGGATCAGATGTTTGCATGTTGTGGAATGTGCCCTGAGGGGTCCTTGCGAGTAATACGTAATGGTGTCAATGTGGAGAAACTTCTGAGGACAGATCCTATTTATCATGGTGTTACTGGTTTGTGGACCTTGAGAATGAAGAGAACTGATGCTTATCACTCTTTTCTTGTATTGTCATTCGTTGAGGAAACCAGAATACTATCAGTAGGACTCAGTTTTAATGACATCTGTGATGCTGTAGGATTTCAGACTGATGTTTGCACTCTGGCATGTGGTTTAGTAGCTGATGGTTTGCTTGTGCAAATTCACAGCAAATGTGTAAAGCTTTGTTTACCTACAGCATGTGCTCACCCTGAGGGAACTTTGCTCCCGTCTCCTGTTTGTGCTGACTGGTATCCTGATGTCACTATCAGTGTTGGTGCTGTTGGACATAATGTTGTTGTTGTTGCTACATCGAATCCTTGTTGCCTTTACATACTTGGGGTCAGATCATTATCTTCGTTTCAGTATGAGCTGTATGAGATACAACATGTCCAGTTGCATTATGAAGTATCTTGCATATCTATTCCACAAGAGGACTGGAGACTTGATAATTCATCTTCTAGTTGTGCAACGAGTGGTGATTTTCGGAAAGATTTTGCAGCTAATATTCGCAAGTTTGCTGTAATTGGAACTCACGAACCTTCTGTGCATATCATTTCGTTGGAACCTGGAGAAGCATTTCAGCAACTTGCTGTTGGGCATATATCAGTAAACAACGCGCTCGGCACTCCTATCAGTGGTTGTATACCTGAAAATGTTAGGTTTGTAGCAGCTGCAAGGTTCTACATTCTTGCAGGACTAAGGAATGGAATGCTACTGAGGTTTGAGTCACAAACAAGCAAAGGGCATTGTTTCCCTGGTTCCTTTTACAAGGAGTCCTCTACCCCTTGTGATGATACATCCCTTATGTTGATTGCTGTACGGCGTATTGGAATCACTCCTGTAGTTTTGGTACCGTTGCATGACAGAGCCAATGCTGACATTATTGTTCTCGGTGATAGGCCTTGGCTATTGCAATCTGCTAGACACAGCTTGGCATATTCATCCATCTCATTTCTGCCTGCTTCCCATGTGACACCAGTGTCTTCTACTGATTGCCCCAGTGGTCTGCTGTTTGTTTCGGAGAACTGTTTGCATTTGGTTGAAATGGTTCATGGCAAAAGACTAAATGCACAGAAGTTTTCGATTGGAGGGACTCCAAGGAAAGTGTTATACCACAGTGATAGTAGAACACTGTTGGTGCTGAGAACTGGGTTAACCAGTGTATCCTGTTCTTCAGATATTGTTCAAATAGATCCAAGTAATGGGGCATTGCTTTCCAGATTCAAGTGCGAACCTGGTGAAACAGCAAAATGCATGCAAATTGCAAAGATAGGGAATGATCAAGTATTAATCGTTGGAACTAGTAAGTCCAATGGCCGACCAATGATGCCAAACGGTGAAGCAGAAAGTATCAAGGGTCGTCTAATTCTTTTAAGCTTGGAGACTATTGAAAGTCCTCGTGAGAGCGGTTCGTTTACTGCAGCTTCTAATTTGAATTCATCTCATGCTGGTTCTCCATTCCCGGAATTTGTTGGATATGCAGCAGAGGAGCTGTCTAGTAACAGCATGTGCAGCAGTCCTGATGAAGTTTGCTGCAATCAAATTCAACCTGAACTCATGGCAGGCCATTTGAGATCACTAGTCCAACATACATTCAATGGCGCTGTTCTTGCTGTACATCCATATCTAGATAGATATGTGTTGGCAGCAGCTGGAAATGTGCTTTTTGTATTTGGATTTTTAAATGAAAGTCCCCATCGGATAAAAAAGTATACTACGAGTAGGACACGATTTACAATAACCTGTTTGAAGACGTATGCATCACGGATTGCAGTGGGTGATTGCCGCGATGGTGTTCTCTTCTATTCTTATCATGAGAATCTTAGGAAGTTGGAACTGATCTATTCTGATCCTGCCCAACGGCTAGTGGGTGATGTTGCCCTTTTGAGCTGTGAAACTGCTGTTGTATCAGATCGACGAGGGAGCATATCTGTACTGTCTTGCCCAAGATTAGAAGTATCCGAAAGTCCAGAGAAGAACCTAGCGGTGCATTGTTCATTTTACATGGGTGAAACAGCTATGAGCATCCAGAAGGTTGCATTTAAGCATTGGCTTCCAATTGATGATTTGACAGAACCAGTGTTGGAGTCGGTATACAACTGTGTCGTCGCAAGCACTCTGCTGGGGAGTATCTTTGTTATGATTCCACTCACAAGTGAGGAGCATCAAATGTTGCAAGATGTCCAAGAAAGACTTTCGGTTCACCCATTGACTGCTCCACTCTTAGGAAATGATCATGCAGAGTTTCGTCGCCGTGGTATCCCGTCGGGAGTACCGCCCATTCTGGATGGCGACATGCTTGTGCAGTTCCTGGAGCTCACCAGCGAACAACAGCACGATGTTCTCAATATTGTTTCTCCTGGAAAGAAGAGGCAACACGATATCTCGGTTTTCCAGGTCATGCGAGCATTAGAGAGGGTGCATTACGCACTCAACTGA
Protein Sequence
- >LOC_Os07g10390.1
MQGHPRLIFCLDDGEFHLLEFSLDMEGVKVLPECVHRGLPCKPLLWMDKGMVVGFVEMGDGMILQLENNRLVHKSAIQNVAPILDLAIADHHGEKQDQMFACCGMCPEGSLRVIRNGVNVEKLLRTDPIYHGVTGLWTLRMKRTDAYHSFLVLSFVEETRILSVGLSFNDICDAVGFQTDVCTLACGLVADGLLVQIHSKCVKLCLPTACAHPEGTLLPSPVCADWYPDVTISVGAVGHNVVVVATSNPCCLYILGVRSLSSFQYELYEIQHVQLHYEVSCISIPQEDWRLDNSSSSCATSGDFRKDFAANIRKFAVIGTHEPSVHIISLEPGEAFQQLAVGHISVNNALGTPISGCIPENVRFVAAARFYILAGLRNGMLLRFESQTSKGHCFPGSFYKESSTPCDDTSLMLIAVRRIGITPVVLVPLHDRANADIIVLGDRPWLLQSARHSLAYSSISFLPASHVTPVSSTDCPSGLLFVSENCLHLVEMVHGKRLNAQKFSIGGTPRKVLYHSDSRTLLVLRTGLTSVSCSSDIVQIDPSNGALLSRFKCEPGETAKCMQIAKIGNDQVLIVGTSKSNGRPMMPNGEAESIKGRLILLSLETIESPRESGSFTAASNLNSSHAGSPFPEFVGYAAEELSSNSMCSSPDEVCCNQIQPELMAGHLRSLVQHTFNGAVLAVHPYLDRYVLAAAGNVLFVFGFLNESPHRIKKYTTSRTRFTITCLKTYASRIAVGDCRDGVLFYSYHENLRKLELIYSDPAQRLVGDVALLSCETAVVSDRRGSISVLSCPRLEVSESPEKNLAVHCSFYMGETAMSIQKVAFKHWLPIDDLTEPVLESVYNCVVASTLLGSIFVMIPLTSEEHQMLQDVQERLSVHPLTAPLLGNDHAEFRRRGIPSGVPPILDGDMLVQFLELTSEQQHDVLNIVSPGKKRQHDISVFQVMRALERVHYALN*