Gene Details:

Functional Descriptions:

  • On the other hand, plants overexpressing OsNek3 occasionally produced a peculiar pollen structure in which the outer cell wall of four pollen grains fused together even at the mature pollen stages, which resembled that of quartet mutants in Arabidopsis.
  • OsNek3 was preferentially expressed in mature pollen.
  • A knockout mutant with Tos17 inserted into OsNek3 did not show any pollen-defective phenotype.
  • OsNek3 was shown to interact with a LIM domain-containing protein, OsPLIM2b, whose expression was strongly specific in mature pollen, suggesting that OsNek3 might play a role in pollen germination.
  • Cytoplasmic male sterility-related protein kinase, OsNek3, is regulated downstream of mitochondrial protein phosphatase 2C, DCW11.
  • These results suggest that OsNek3 is downstream of DCW11 in retrograde signaling from the mitochondria to the nucleus and is involved in CW-CMS.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os07g08000.1
    AGAAGTTTGAAACCTCTCACAAAAACAGGCAGCCCTCCATTAGCAAGAGTGCCCAATCTATCTGTCTTCTCCATAAGAGAGCATCTCAGGTGTAGTTTTTAGTTTCTCTCTCTAAAAATGGGAGGCAACTGAGGAAGAAGGAAGAACACGGCCTTCATTTCATCCAATTGAGTCAAGAGAGGAAATCTGAATTTGGGGGCGGCCAAGAAATGGAGCAGTATGAGGTGCTGGAGCAGATTGGGAAGGGGTCATTTGGCTCTGCCCTCCTGGTGAGACACAAGGTTGAGAAGAAGAGGTATGTCTTGAAGAAGATCAGACTTGCCCGTCAGACCGACAGATGCCGCCGATCAGCACACCAAGAGATGGAGCTCATTGCAAAAGTAAGGAACCCTTACATTGTGGAATACAAAGATTCATGGGTGGAGAAGGGTTGTTATGTGTGCATCGTGATTGGTTACTGTGAAGGAGGAGACATGTCAGAAGCCATTAAGAAGGCCAACAGTAATTATTTCTCAGAAGAGAGACTTTGTATGTGGCTTGTGCAGCTCCTGATGGCACTTGATTACTTGCATGTCAACCATATCCTTCATCGTGATGTGAAGTGCTCAAATATATTTCTGACGAAGGATCAAAACATACGGCTCGGTGATTTTGGTTTGGCCAAAGTATTGACTTCTGACGATTTAACTTCATCAGTTGTGGGTACTCCCAGCTACATGTGCCCTGAACTTCTTGCTGACATTCCATATGGTTCCAAGTCTGACATATGGTCACTAGGCTGTTGCCTCTATGAGATGACTGCTTTGAAGCCTGCATTTAAAGCATTTGATATGCAAACACTGATAAACAAGATAAGCAAGTCGGTTCTCGCTCCTCTACCGACCATCTACTCCGGTGCATTTAGGGGGCTCATCAAGAGCATGCTGCGAAAAAGTCCAGATCACAGACCAAGTGCTGCAGAATTGCTTAAGCACCCACACCTTCAGCCCTTTGTACTGGAACTCCAATTGAAGTCGAGTCCCGCTCGCAATCTTTTCCCGGATACAAATAAGGCTTCATGTTCTGATGATGAAAACAATTGGAAGGCTAAGTACAGCAAGAGCCATTCATTTAAAGTAGACAGGATTGTAAAAGTTGACAAAGTTGCTGCTAACAATGGCCATCCTAGTTCTACTGGAACTGCAAAAGATTACCAAGAATTGCTAAAACAACCAATGGATGAGTTGTTAGGACAATTAACCGAGAAAGTTGTTGATGAAGTGATACATGGAAATCATTCAAGAGTAACAAAATCTCCAGCTCCTACTCCAAGAAGGGCATCATCAACTCCTAGAATACGACTAGAACCTTCAAAGACATTTCATGCTAGAGCAGCTGAGACTCCACCATCCAAATGTTCACTGGAACGAGCAAGTCAACCTACAAGACGAGCATCAACCCCGGTGAACATGTTGCAAACTCCTGAGAAACGGCAGGGCGCCGACATCCTCACCAGACTAAAATCACCCGACGTTTCAGTGAACTCTCCTCGAATCGACAGAATCGCCGAATTCCCGATACCATCATTTGATGATGAACAATTGCACCCCACAACCAAGCTCAAGCTCTATCCACCATCCATCACTGACCAATCGATCACCAAGGACAAGTGCACATTCCAGGTTCTCCGAAGCGACAGCAGCAAGAACCACACCGGAGACAGTTCTGATCCCAGCATTCTTGGCACTGACAGTAATCCACTCATCACGAGCTCATCGGATTGGATGAAGCAGAGGAGATTCGACACGACGTCGTACCGTCAGAGAGCCGAGGCACTGGAAGGATTGCTGGAGTTCAGTGCACAGCTGCTGCAGCAGGAGAGGTTTGAGGAGCTGGGGATCTTGCTGAAGCCATTTGGGCCAGGGAAGGCGTCTCCGAGAGAGACGGCCATATGGTTGTCAAAGAGCTTCAAAGGGACTGGATTGTGATTAATCAGAGTCCTGCAAATGCAAAAAAAAGAAAAAAAAATCTGAACCTAGAATAATTTTGTGTCTTTGATTAGATTCAGGCTGCTCTGTACAGTTTCAGTTTTGTGGTAATATCCTCTTTTCGTCTTTGATTTTCGCGAAATGTTTGAAACACTCCGTTGTTTGGCGAGAGAAGCTCCATTTTTGTGTATAGCAAATAATTGCTCAAGAATGGGGAAGCACCGGTGGTGTGGTGGTGAAATCAGGAATGAATTTACTCCGTATTCTGAGGG
CDS Sequence
  • >LOC_Os07g08000.1
    ATGGAGCAGTATGAGGTGCTGGAGCAGATTGGGAAGGGGTCATTTGGCTCTGCCCTCCTGGTGAGACACAAGGTTGAGAAGAAGAGGTATGTCTTGAAGAAGATCAGACTTGCCCGTCAGACCGACAGATGCCGCCGATCAGCACACCAAGAGATGGAGCTCATTGCAAAAGTAAGGAACCCTTACATTGTGGAATACAAAGATTCATGGGTGGAGAAGGGTTGTTATGTGTGCATCGTGATTGGTTACTGTGAAGGAGGAGACATGTCAGAAGCCATTAAGAAGGCCAACAGTAATTATTTCTCAGAAGAGAGACTTTGTATGTGGCTTGTGCAGCTCCTGATGGCACTTGATTACTTGCATGTCAACCATATCCTTCATCGTGATGTGAAGTGCTCAAATATATTTCTGACGAAGGATCAAAACATACGGCTCGGTGATTTTGGTTTGGCCAAAGTATTGACTTCTGACGATTTAACTTCATCAGTTGTGGGTACTCCCAGCTACATGTGCCCTGAACTTCTTGCTGACATTCCATATGGTTCCAAGTCTGACATATGGTCACTAGGCTGTTGCCTCTATGAGATGACTGCTTTGAAGCCTGCATTTAAAGCATTTGATATGCAAACACTGATAAACAAGATAAGCAAGTCGGTTCTCGCTCCTCTACCGACCATCTACTCCGGTGCATTTAGGGGGCTCATCAAGAGCATGCTGCGAAAAAGTCCAGATCACAGACCAAGTGCTGCAGAATTGCTTAAGCACCCACACCTTCAGCCCTTTGTACTGGAACTCCAATTGAAGTCGAGTCCCGCTCGCAATCTTTTCCCGGATACAAATAAGGCTTCATGTTCTGATGATGAAAACAATTGGAAGGCTAAGTACAGCAAGAGCCATTCATTTAAAGTAGACAGGATTGTAAAAGTTGACAAAGTTGCTGCTAACAATGGCCATCCTAGTTCTACTGGAACTGCAAAAGATTACCAAGAATTGCTAAAACAACCAATGGATGAGTTGTTAGGACAATTAACCGAGAAAGTTGTTGATGAAGTGATACATGGAAATCATTCAAGAGTAACAAAATCTCCAGCTCCTACTCCAAGAAGGGCATCATCAACTCCTAGAATACGACTAGAACCTTCAAAGACATTTCATGCTAGAGCAGCTGAGACTCCACCATCCAAATGTTCACTGGAACGAGCAAGTCAACCTACAAGACGAGCATCAACCCCGGTGAACATGTTGCAAACTCCTGAGAAACGGCAGGGCGCCGACATCCTCACCAGACTAAAATCACCCGACGTTTCAGTGAACTCTCCTCGAATCGACAGAATCGCCGAATTCCCGATACCATCATTTGATGATGAACAATTGCACCCCACAACCAAGCTCAAGCTCTATCCACCATCCATCACTGACCAATCGATCACCAAGGACAAGTGCACATTCCAGGTTCTCCGAAGCGACAGCAGCAAGAACCACACCGGAGACAGTTCTGATCCCAGCATTCTTGGCACTGACAGTAATCCACTCATCACGAGCTCATCGGATTGGATGAAGCAGAGGAGATTCGACACGACGTCGTACCGTCAGAGAGCCGAGGCACTGGAAGGATTGCTGGAGTTCAGTGCACAGCTGCTGCAGCAGGAGAGGTTTGAGGAGCTGGGGATCTTGCTGAAGCCATTTGGGCCAGGGAAGGCGTCTCCGAGAGAGACGGCCATATGGTTGTCAAAGAGCTTCAAAGGGACTGGATTGTGA
Protein Sequence
  • >LOC_Os07g08000.1
    MEQYEVLEQIGKGSFGSALLVRHKVEKKRYVLKKIRLARQTDRCRRSAHQEMELIAKVRNPYIVEYKDSWVEKGCYVCIVIGYCEGGDMSEAIKKANSNYFSEERLCMWLVQLLMALDYLHVNHILHRDVKCSNIFLTKDQNIRLGDFGLAKVLTSDDLTSSVVGTPSYMCPELLADIPYGSKSDIWSLGCCLYEMTALKPAFKAFDMQTLINKISKSVLAPLPTIYSGAFRGLIKSMLRKSPDHRPSAAELLKHPHLQPFVLELQLKSSPARNLFPDTNKASCSDDENNWKAKYSKSHSFKVDRIVKVDKVAANNGHPSSTGTAKDYQELLKQPMDELLGQLTEKVVDEVIHGNHSRVTKSPAPTPRRASSTPRIRLEPSKTFHARAAETPPSKCSLERASQPTRRASTPVNMLQTPEKRQGADILTRLKSPDVSVNSPRIDRIAEFPIPSFDDEQLHPTTKLKLYPPSITDQSITKDKCTFQVLRSDSSKNHTGDSSDPSILGTDSNPLITSSSDWMKQRRFDTTSYRQRAEALEGLLEFSAQLLQQERFEELGILLKPFGPGKASPRETAIWLSKSFKGTGL*