Information report for WAF1;OsHEN1
Gene Details
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Functional Descriptions
- About half of the WAF1 mutants showed seedling lethality due to defects in SAM maintenance, but the rest survived to the reproductive phase and exhibited pleiotropic phenotypes in leaf morphology and floral development.
- The rice wavy leaf1 (WAF1) mutant has been identified as an embryonic mutant resembling shoot organization1 (sho1) and sho2, homologs of a loss-of-function mutant of DICER-LIKE4 and a hypomorphic mutant of ARGONAUTE7, respectively, which both act in the ta-siRNA production pathway.
- Our results indicate that stabilization of small RNAs by WAF1 is indispensable for rice development, especially for SAM maintenance and leaf morphogenesis governed by the ta-siRNA pathway.
Functional Keywords
- floral , leaf , reproductive , shoot , seedling
Literature and News
- WAVY LEAF1, an ortholog of Arabidopsis HEN1, regulates shoot development by maintaining MicroRNA and trans-acting small interfering RNA accumulation in rice . DOI: 10.1104/pp.110.160234 ; PMID: 20805329
- DCL2 is highly expressed in the egg cell in both rice and Arabidopsis . DOI: 10.4161/psb.6.4.14650 ; PMID: 21673515
Gene Resources
- UniProt: Q0D8F3
- EMBL: AK111609, AP014963
- AlphaFoldDB: Q0D8F3
- EnsemblPlants: Os07t0164000-01
- Gramene: Os07t0164000-01
- KEGG: dosa:Os07g0164000
- Orthologous matrix: ELLWEWP
- InterPro: IPR013217, IPR026610, IPR029063
- PANTHER: PTHR21404, PTHR21404:SF3
- SUPFAM: SSF53335, SSF54768
- Gene3D: 3.30.160.20, 3.40.50.150
- SWISS-MODEL: Q0D8F3
- eggNOG: KOG1045
Sequences
cDNA Sequence
- >LOC_Os07g06970.1
TTCCCTTCCCTAAACCCCACTTCACCCGACTCCTCGAATCCTCCTCGCCTCGCTTCCGATTTTTCTGGGGGGTTTAGCGCCTCCCCCAATTCGGCGACCACCCCCTCCCGATTCCGCCTCAGTTCCTGCGTCGATCGATTGCGAAAATCCCCTTCGTTACCAATCGCATTTGTTTGTTTGTTTTTGTTTGTTTTTTTTGGGCGTTTGATGCCGTTGCGGCTGAGAGGCGGCGGGGGATCGAAGCGAGGGATGCCGGCGGCGCCGACGGTGACGCCCAAGGCGGTGATCCACCAGAAGTACGGCGCCAAGGCGTGCTACAGCGTGGAGGAGGTGCGGGAGGCCGTCGACGGCGGCTGCCCCGGCCTCGCGCTGCCGCAGCAGACCAGGAGCGTCTACCGCTGCTCGCTCGACATCCCCGGCCTCACCGTCGTCACCCCGGGCACCTTCGTCAGGAAGAAGGACGCCGAGCAAGCCGCCGCGCAGATTGCCCTCGACAAGCTTGGTATTCAGCCCACTGCAAATGCTCCCAGTACTCCTGAAGAAGCATGGGATGAACTTATTGCTCGCATATCTGGTTTTTTTACAGATGAGAACTTCCCCTCGTCATCTCATCCTCTTATTGGCCACATGTGTGTGACATTCAGAAGAACCGGGGACCGCTTTGGTATGATTCCTATGTCAGCAATTGCTGCATGTGATGTGAAGGTCATTGGACTGTGTAAACTTATTGACCCCAAAGCGGAGTTTGATCCTCTCTTGGTTTTATCATTAATCTATAATGCTGCCAAAAAATCCCCTGGTGTCTCTGTCAGTGATAGCAACTTCTGGATTTGGAGTCAGAAGCCTTATTCCCCTGAGGCTGTTGATTTGGCACTTCAGCATTGGTCTGGCATAACGGATCCCATAGAAGTAGATGGGATTTTTGTTCCTTGTATGATGGAGGATGAACCTAAGACAATAAGACTTACTTTATCACATAATGAACATTACATGGGTGATATAGTGTCTAAGCTCTCTGCAAGTGATTCCTCCCACGCTGTTGTTTCAAGAACTGTTGGCAAAGCATCTTCTGAGATAAGGCTATATTTCTCAGCTCCAAATGTGCAATTTGTTTCTGAGATATCACACAATGTGGTTTCTTCTCTTGGAGATGGCTATATGGAGAGCCTAATAAATAAGCGAGCTTCCTTCATTTCTGGTCAAACAATTTATGGAGATGCAATTTTGGCTAATGTTGGATATACAAGGAGAGACTCAGAGCTTCATACTGAAGATGTTACCTTATCCAACTATTACAGGATTCTTCTGGGCAAATCACCAGATGGGAATTACAAGATATCCAGGGATTCAATTCTGGTGGCAGAGCTTCCATCTGTGTATTCTCGTTCTAGTTGGAAGGGTCTCTCTCCACGAGATCTTCTCTGCTCATTTTGTCGCCTTCACAGACTAGCAGAGCCCTACTTCGCTGTCAATAGGTGTGCTAGTGATGGAAAGAATGACAAGGAAAACCCAGATATGTTCAAATGTGATGTCAAAATATATTCAAAGAAGCAGGAGCTTTTATTGGAGTATTCGACAGCTGATACTTGGAGCAAGGAATCTGATGCCATTCACAATTCTTCATTGAAGGTGTTGATTTGGTTCTGTAGTTATTTCAAGCAACCGAACAAGCATGTACTGAAGCTCTCTCATTCTAAGAGCACTGATGGCTTCACAATATGTCCAGATAATTTTTTGCATGAATTTGCCATGTTCTTATCTATTTATGGCAACAGAGGAGGTGATGACTCAAGTGCATGCAGTACAGTTGGATCGCTTTCTATGGATACCTCAAAACAAAAGCTTGAAAACAATGCAGTTTTAGCACACATTGATGGTCCAGATTCTGGTGTTTTTCCATCCCATGGATCATTGACTTGCATAAGCTATACTGCTTCCTTGGTGGTGAAGGACAAAACAAACAGATATACGCTAGAAAGCAACAATGAGTTTGAGTTTGAGATAGGGACTGGGGCTGTTAAGAATCAGATTGAATCATGCGTGAGTCAGCTGTCAGTTAATCAAAGCGCATGTTTCATAGCTGAATTGCCTCCTAAGGATTTGATCCTTGCTGCAGCTAATGAGTTTTCTCATGACCTTTCCAAGATATCTAGAGACAATTGCTTCTTGGAGTTCTCTGTAAAGGTATTGCAGGTAACTGAACCATTAGAAGATAGAATGGAAAAAGCCTTGTTTAATCCTCCTTTATCTAAACAAAGAGTGGAATTCGCTGTTCGGTACATCAATGAGCTGCATGCTACCACATTGGTTGATTTTGGCTGTGGATCTGGTAGCCTTCTTGATTCATTGCTTGAGCATCCAACAACCCTTGAAAAAGTTGTCGGTGTTGACATTTCTCGGAAGGGTCTCACACGAGCAGCAAAGAGCCTTCATCAGAAGCTGAGCAAGAAATCGTTGATGCAGACAAGTGTTCCAACGGCTGTGCTCTATGATGGTTCTATAACGGATTTTGATTCTCGCTTATACAGATTTGACATTGGCACTTGTCTTGAGGTGATTGAGCACGTAGAGGAGGACCAAGCAAGTTTATGTGGCGACGTCGTCTTGAGTTCGTTCTGCCCTACGGTGCTGATTGTTTCAACACCCAACTATGAATACAACCCTATCCTCCAGAGATCAGCTATGCCAAACAAGGAAGAAGAACCAGAAGAAAACGCTGGTCCTTGCAAGTTCCGAAACCATGACCACAAGTTCGAGTGGACAAGATCCCAGTTCCAGCACTGGGCTACCGGACTCGCAGAAAAGCACAACTACAGCGTCGAGTTCAGTGGCGTTGGCGGATCAGGCGATGAACCAGGCTTCGCTTCCCAGATCGCTGTCTTCAGAAGAATGGCGTCGGGTCAAGATGAGGTTTGCCAGGAGGGAGAGCTGCATCAACCTTATGAGCTTCTCTGGGAATGGCCAAATGCATCGTTGCCATCTCACTGAGTTCTTGTGGTAGGGAGACACGACAACTCTATTAACTCTTCACCATGCTTGTAACTGAGATGGAGATTCTGATTAGTACTGTAGTTGGCATTAACTTGTCGTCAGTAGGAAACTCAACTCAAGATCGTGTCCCCGGATGTTGGTGGGTTAATGCCTTGGGCTGTGTTCTAAAGTCAATGTTGCCAATTTCACCAATCTTGTTTTCCACGTGCGTATTTTCCGAATAGCTAAA
CDS Sequence
- >LOC_Os07g06970.1
ATGCCGTTGCGGCTGAGAGGCGGCGGGGGATCGAAGCGAGGGATGCCGGCGGCGCCGACGGTGACGCCCAAGGCGGTGATCCACCAGAAGTACGGCGCCAAGGCGTGCTACAGCGTGGAGGAGGTGCGGGAGGCCGTCGACGGCGGCTGCCCCGGCCTCGCGCTGCCGCAGCAGACCAGGAGCGTCTACCGCTGCTCGCTCGACATCCCCGGCCTCACCGTCGTCACCCCGGGCACCTTCGTCAGGAAGAAGGACGCCGAGCAAGCCGCCGCGCAGATTGCCCTCGACAAGCTTGGTATTCAGCCCACTGCAAATGCTCCCAGTACTCCTGAAGAAGCATGGGATGAACTTATTGCTCGCATATCTGGTTTTTTTACAGATGAGAACTTCCCCTCGTCATCTCATCCTCTTATTGGCCACATGTGTGTGACATTCAGAAGAACCGGGGACCGCTTTGGTATGATTCCTATGTCAGCAATTGCTGCATGTGATGTGAAGGTCATTGGACTGTGTAAACTTATTGACCCCAAAGCGGAGTTTGATCCTCTCTTGGTTTTATCATTAATCTATAATGCTGCCAAAAAATCCCCTGGTGTCTCTGTCAGTGATAGCAACTTCTGGATTTGGAGTCAGAAGCCTTATTCCCCTGAGGCTGTTGATTTGGCACTTCAGCATTGGTCTGGCATAACGGATCCCATAGAAGTAGATGGGATTTTTGTTCCTTGTATGATGGAGGATGAACCTAAGACAATAAGACTTACTTTATCACATAATGAACATTACATGGGTGATATAGTGTCTAAGCTCTCTGCAAGTGATTCCTCCCACGCTGTTGTTTCAAGAACTGTTGGCAAAGCATCTTCTGAGATAAGGCTATATTTCTCAGCTCCAAATGTGCAATTTGTTTCTGAGATATCACACAATGTGGTTTCTTCTCTTGGAGATGGCTATATGGAGAGCCTAATAAATAAGCGAGCTTCCTTCATTTCTGGTCAAACAATTTATGGAGATGCAATTTTGGCTAATGTTGGATATACAAGGAGAGACTCAGAGCTTCATACTGAAGATGTTACCTTATCCAACTATTACAGGATTCTTCTGGGCAAATCACCAGATGGGAATTACAAGATATCCAGGGATTCAATTCTGGTGGCAGAGCTTCCATCTGTGTATTCTCGTTCTAGTTGGAAGGGTCTCTCTCCACGAGATCTTCTCTGCTCATTTTGTCGCCTTCACAGACTAGCAGAGCCCTACTTCGCTGTCAATAGGTGTGCTAGTGATGGAAAGAATGACAAGGAAAACCCAGATATGTTCAAATGTGATGTCAAAATATATTCAAAGAAGCAGGAGCTTTTATTGGAGTATTCGACAGCTGATACTTGGAGCAAGGAATCTGATGCCATTCACAATTCTTCATTGAAGGTGTTGATTTGGTTCTGTAGTTATTTCAAGCAACCGAACAAGCATGTACTGAAGCTCTCTCATTCTAAGAGCACTGATGGCTTCACAATATGTCCAGATAATTTTTTGCATGAATTTGCCATGTTCTTATCTATTTATGGCAACAGAGGAGGTGATGACTCAAGTGCATGCAGTACAGTTGGATCGCTTTCTATGGATACCTCAAAACAAAAGCTTGAAAACAATGCAGTTTTAGCACACATTGATGGTCCAGATTCTGGTGTTTTTCCATCCCATGGATCATTGACTTGCATAAGCTATACTGCTTCCTTGGTGGTGAAGGACAAAACAAACAGATATACGCTAGAAAGCAACAATGAGTTTGAGTTTGAGATAGGGACTGGGGCTGTTAAGAATCAGATTGAATCATGCGTGAGTCAGCTGTCAGTTAATCAAAGCGCATGTTTCATAGCTGAATTGCCTCCTAAGGATTTGATCCTTGCTGCAGCTAATGAGTTTTCTCATGACCTTTCCAAGATATCTAGAGACAATTGCTTCTTGGAGTTCTCTGTAAAGGTATTGCAGGTAACTGAACCATTAGAAGATAGAATGGAAAAAGCCTTGTTTAATCCTCCTTTATCTAAACAAAGAGTGGAATTCGCTGTTCGGTACATCAATGAGCTGCATGCTACCACATTGGTTGATTTTGGCTGTGGATCTGGTAGCCTTCTTGATTCATTGCTTGAGCATCCAACAACCCTTGAAAAAGTTGTCGGTGTTGACATTTCTCGGAAGGGTCTCACACGAGCAGCAAAGAGCCTTCATCAGAAGCTGAGCAAGAAATCGTTGATGCAGACAAGTGTTCCAACGGCTGTGCTCTATGATGGTTCTATAACGGATTTTGATTCTCGCTTATACAGATTTGACATTGGCACTTGTCTTGAGGTGATTGAGCACGTAGAGGAGGACCAAGCAAGTTTATGTGGCGACGTCGTCTTGAGTTCGTTCTGCCCTACGGTGCTGATTGTTTCAACACCCAACTATGAATACAACCCTATCCTCCAGAGATCAGCTATGCCAAACAAGGAAGAAGAACCAGAAGAAAACGCTGGTCCTTGCAAGTTCCGAAACCATGACCACAAGTTCGAGTGGACAAGATCCCAGTTCCAGCACTGGGCTACCGGACTCGCAGAAAAGCACAACTACAGCGTCGAGTTCAGTGGCGTTGGCGGATCAGGCGATGAACCAGGCTTCGCTTCCCAGATCGCTGTCTTCAGAAGAATGGCGTCGGGTCAAGATGAGGTTTGCCAGGAGGGAGAGCTGCATCAACCTTATGAGCTTCTCTGGGAATGGCCAAATGCATCGTTGCCATCTCACTGA
Protein Sequence
- >LOC_Os07g06970.1
MPLRLRGGGGSKRGMPAAPTVTPKAVIHQKYGAKACYSVEEVREAVDGGCPGLALPQQTRSVYRCSLDIPGLTVVTPGTFVRKKDAEQAAAQIALDKLGIQPTANAPSTPEEAWDELIARISGFFTDENFPSSSHPLIGHMCVTFRRTGDRFGMIPMSAIAACDVKVIGLCKLIDPKAEFDPLLVLSLIYNAAKKSPGVSVSDSNFWIWSQKPYSPEAVDLALQHWSGITDPIEVDGIFVPCMMEDEPKTIRLTLSHNEHYMGDIVSKLSASDSSHAVVSRTVGKASSEIRLYFSAPNVQFVSEISHNVVSSLGDGYMESLINKRASFISGQTIYGDAILANVGYTRRDSELHTEDVTLSNYYRILLGKSPDGNYKISRDSILVAELPSVYSRSSWKGLSPRDLLCSFCRLHRLAEPYFAVNRCASDGKNDKENPDMFKCDVKIYSKKQELLLEYSTADTWSKESDAIHNSSLKVLIWFCSYFKQPNKHVLKLSHSKSTDGFTICPDNFLHEFAMFLSIYGNRGGDDSSACSTVGSLSMDTSKQKLENNAVLAHIDGPDSGVFPSHGSLTCISYTASLVVKDKTNRYTLESNNEFEFEIGTGAVKNQIESCVSQLSVNQSACFIAELPPKDLILAAANEFSHDLSKISRDNCFLEFSVKVLQVTEPLEDRMEKALFNPPLSKQRVEFAVRYINELHATTLVDFGCGSGSLLDSLLEHPTTLEKVVGVDISRKGLTRAAKSLHQKLSKKSLMQTSVPTAVLYDGSITDFDSRLYRFDIGTCLEVIEHVEEDQASLCGDVVLSSFCPTVLIVSTPNYEYNPILQRSAMPNKEEEPEENAGPCKFRNHDHKFEWTRSQFQHWATGLAEKHNYSVEFSGVGGSGDEPGFASQIAVFRRMASGQDEVCQEGELHQPYELLWEWPNASLPSH*