Gene Details:

• MSU gene ID: LOC_Os07g06740
• RAPdb gene ID: Os07g0161600
• Gene Symbol: OsCPK17
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• A comparative phosphoproteomic approach led to the identification of six potential in vivo OsCPK17 targets, which are associated with sugar and nitrogen metabolism, and with osmotic regulation.
• Here we test the hypothesis that OsCPK17 plays a role in rice cold stress response by analyzing OsCPK17 knockout, silencing, and overexpressing rice lines under low temperature.
• Altered OsCPK17 gene expression compromises cold tolerance performance, without affecting the expression of key cold stress-inducible genes.
• \Rice calcium-dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose phosphate synthase and is required for a proper cold stress response.
• Altogether, our data indicates that OsCPK17 is required for a proper cold stress response in rice, likely affecting the activity of membrane channels and sugar metabolism.
• To test direct interaction, in vitro kinase assays were performed, showing that the sucrose phosphate synthase OsSPS4, and the aquaporin OsPIP2;1/OsPIP2;6 are phosphorylated by OsCPK17 in a calcium-dependent manner.
• The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications.
• Our analysis indicated that OsCPK17 activity depends on its structural rearrangement induced by calcium binding, and that the protein can be autophosphorylated.

Function-related keywords:

• nitrogen ,  temperature ,  tolerance ,  cold-tolerance ,  cold-stress ,  stress ,  plasma-membrane ,  sugar ,  Kinase ,  stress-response ,  phosphate ,  sucrose ,  protein-kinase ,  calcium

Literature:

• Rice calcium-dependent protein kinase OsCPK17 targets plasma membrane intrinsic protein and sucrose-phosphate synthase and is required for a proper cold stress response .  DOI:  10.1111/pce.12916 ;  PMID:  28102545
• The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications .  DOI:  10.1016/j.bbamcr.2017.10.010 ;  PMID:  29100789

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: