Gene Details:

• MSU gene ID: LOC_Os07g05940
• RAPdb gene ID: Os07g0154100
• Gene Symbol: OsNCED4
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C.
• Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions.
• The 9-cis-epoxycarotenoid dioxygenases OsNCED4 was cloned from rice in conjunction with OsNCED 1-3 and 5, of which 3 has been shown to function in ABA biosynthesis and alteration of leaf morphology.
• Heterologous expression of OsNCED4 in Arabidopsis increased ABA levels and altered plant size and leaf shape, delayed seed germination, caused sugar oversensitivity in post-germination growth, and enhanced tolerance to drought.
• At the one-leaf stage, similar regulation of OsNCED3 and OsNCED4 gene expression in roots or leaves in response to moderate salt stress (150<U+00A0>mM NaCl) was observed.
• Thus, transgenic Arabidopsis plants overexpressing OsNCED4 were generated in the 129B08/nced3 mutant background to explore OsNCED4 function in ABA biosynthesis.
• Like OsNCED3, OsNCED4 is functionally active in ABA biosynthesis in rice.
• OsNCED3 and OsNCED4 might play redundant roles in controlling ABA biosynthesis in rice, as suggested by GUS staining assay, but this should be further analyzed through complementation of rice NCED knockout mutants.
• Furthermore, Overexpression of OsABT decreased the abscisic acid (ABA) content, while downregulating the ABA synthesis genes OsNCED3 and OsNCED4 and upregulating the ABA catabolic gene OsABA8ox2.

Function-related keywords:

• salt ,  drought ,  ABA ,  ethylene ,  leaf ,  seed ,  seed-germination ,  tolerance ,  salt-stress ,  stress ,  leaf-shape ,  sugar ,  ABA-biosynthesis ,  abscisic-acid

Literature:

• The rice ERF transcription factor OsERF922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance .  DOI:  10.1093/jxb/ers079 ;  PMID:  22442415
• Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice .  DOI:  10.1371/journal.pone.0092913 ;  PMID:  24667379
• Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis .  DOI:  10.1093/pcp/pcp022 ;  PMID:  19208695
• Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice .  DOI:  10.1007/s00299-013-1532-0 ;  PMID:  24247850
• Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 (OsNCED4) in Arabidopsis confers sugar oversensitivity and drought tolerance .  DOI:  10.1186/s40529-018-0219-9 ;  PMID:  29335785
• OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage .  DOI:  10.3390/ijms231810656 ;  PMID:  36142568

Related News:

Gene Resources:

• NCBI ID: AY838900
• UniProt accessions:

Sequences: