Gene Details:

• MSU gene ID: LOC_Os07g04560
• RAPdb gene ID: Os07g0138200
• Gene Symbol: ONAC096
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice.
• Here, we report that rice ONAC096 affects grain yield by regulating leaf senescence and panicle number.
• Two independent T-DNA insertion mutants (ONAC096-1 and ONAC096-2) with downregulated ONAC096 expression retained their green leaf color during natural senescence in the field, thus extending their photosynthetic capacity.
• Reverse-transcription quantitative PCR analysis showed that ONAC096 upregulated genes controlling chlorophyll degradation and leaf senescence.
• ONAC096 mediates abscisic acid (ABA)-induced leaf senescence by upregulating the ABA signaling genes ABA INSENSITIVE5 and ENHANCED EM LEVEL.
• ONAC096 expression increased rapidly in rice leaves upon the initiation of aging- and dark-induced senescence.
• Repressed OsCKX2 (encoding cytokinin oxidase/dehydrogenase) expression in the ONAC096 mutants led to a 15% increase in panicle number without affecting grain weight or fertility.
• The ONAC096 mutants showed a 16% increase in grain yield, highlighting the potential for using this gene to increase grain production.
• A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1.
• RNA-seq transcriptomic profiling and qRT-PCR analysis identified a small set of defense and signaling genes that are putatively regulated by ONAC096, and further biochemical analysis validated that ONAC096 could directly bind to the promoters of OsRap2.
• CRISPR/Cas9-mediated knockout of ONAC096 attenuated rice immunity and flg22-induced reactive oxygen species burst and expression of PTI marker genes OsWRKY45 and OsPAL4; by contrast, overexpression of ONAC096 enhanced rice immunity or flg22-induced PTI.
• ONAC096 interacts with ONAC066, which is a positive regulator of rice immunity.
• These results demonstrate that ONAC096 positively contributes to rice immunity against M.
• ONAC096 had the DNA binding ability to NAC recognition sequence and was found to be a nucleus-localized transcriptional activator whose activity depended on its C-terminal.

Function-related keywords:

• leaf ,  leaf-senescence ,  senescence ,  panicle ,  grain ,  grain-yield ,  yield ,  ABA ,  cytokinin ,  abscisic-acid ,  grain-filling ,  fertility ,  grain-weight ,  transcription-factor ,  defense ,  immunity ,  reactive-oxygen-species ,  transcriptional-activator

Literature:

• Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size .  DOI:  10.3389/fpls.2016.01638 ;  PMID:  27872632
• Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice .  DOI:  10.3390/ijms20205241 ;  PMID:  31652646
• A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1 .  DOI:  10.3389/fpls.2021.802758 ;  PMID:  34956298

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: