Information report for ONAC096
Gene Details
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Functional Descriptions
- Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice.
- Here, we report that rice ONAC096 affects grain yield by regulating leaf senescence and panicle number.
- Two independent T-DNA insertion mutants (ONAC096-1 and ONAC096-2) with downregulated ONAC096 expression retained their green leaf color during natural senescence in the field, thus extending their photosynthetic capacity.
- Reverse-transcription quantitative PCR analysis showed that ONAC096 upregulated genes controlling chlorophyll degradation and leaf senescence.
- ONAC096 mediates abscisic acid (ABA)-induced leaf senescence by upregulating the ABA signaling genes ABA INSENSITIVE5 and ENHANCED EM LEVEL.
- ONAC096 expression increased rapidly in rice leaves upon the initiation of aging- and dark-induced senescence.
- Repressed OsCKX2 (encoding cytokinin oxidase/dehydrogenase) expression in the ONAC096 mutants led to a 15% increase in panicle number without affecting grain weight or fertility.
- The ONAC096 mutants showed a 16% increase in grain yield, highlighting the potential for using this gene to increase grain production.
- A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1.
- RNA-seq transcriptomic profiling and qRT-PCR analysis identified a small set of defense and signaling genes that are putatively regulated by ONAC096, and further biochemical analysis validated that ONAC096 could directly bind to the promoters of OsRap2.
- CRISPR/Cas9-mediated knockout of ONAC096 attenuated rice immunity and flg22-induced reactive oxygen species burst and expression of PTI marker genes OsWRKY45 and OsPAL4; by contrast, overexpression of ONAC096 enhanced rice immunity or flg22-induced PTI.
- ONAC096 interacts with ONAC066, which is a positive regulator of rice immunity.
- These results demonstrate that ONAC096 positively contributes to rice immunity against M.
- ONAC096 had the DNA binding ability to NAC recognition sequence and was found to be a nucleus-localized transcriptional activator whose activity depended on its C-terminal.
Functional Keywords
- leaf , leaf-senescence , senescence , panicle , grain , grain-yield , yield , ABA , cytokinin , abscisic-acid , grain-filling , fertility , grain-weight , transcription-factor , defense , immunity , reactive-oxygen-species , transcriptional-activator
Literature and News
- Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size . DOI: 10.3389/fpls.2016.01638 ; PMID: 27872632
- Mutation of ONAC096 Enhances Grain Yield by Increasing Panicle Number and Delaying Leaf Senescence during Grain Filling in Rice . DOI: 10.3390/ijms20205241 ; PMID: 31652646
- A Pathogen-Inducible Rice NAC Transcription Factor ONAC096 Contributes to Immunity Against Magnaprothe oryzae and Xanthomonas oryzae pv. oryzae by Direct Binding to the Promoters of OsRap2.6, OsWRKY62, and OsPAL1 . DOI: 10.3389/fpls.2021.802758 ; PMID: 34956298
Gene Resources
- UniProt: A3BGF0
- EMBL: AP003829, AP004314, CM000144
- EnsemblPlants: Os07t0138200-01
- Gramene: Os07t0138200-01
- InterPro: IPR003441, IPR036093
- PANTHER: PTHR31744, PTHR31744:SF65
- SUPFAM: SSF101941
- PROSITE: PS51005
- Gene3D: 2.170.150.80
- SWISS-MODEL: A3BGF0
Sequences
cDNA Sequence
- >LOC_Os07g04560.1
ATGAAGAGGGGTTGTGAAGATGAACTTGGAGCTGGTGATGTGATTCTACGAGGGGTGGAGGAGGTAGAAGAGGAAGATGATGATGACCTGGTTCTCCCCGGATTCCGGTTCCATCCTACCGACGAGGAGCTAGTGACGTTCTACCTTCGCCGGAAGATCGCCGAGAAGCGGCTGAGCATCGAGATCATCAAGGAGATGGACATCTACAAGCATGATCCCTCCGACTTCCTCAAGACGAGCACGGTGGGCAGCGAGAAGGAATGGTACTTCTTCTGCCTTCGAGGGAGGAAGTACAGGAACAGCATCCGACCCAACCGGGTCACCGGCTCCGGCTTCTGGAAGGCCACCGGAATCGACCGCCCAATCTGCTCTGCCGCCGGCGGCGGCGGCGGCGACTGCATCGGCCTCAAGAAGTCGCTCGTCTACTACCGCGGCAGCGCCGGCAAGGGCACCAAGACCGACTGGATGATGCACGAGTTCCGCCTCCCTCCGCCGCCCGCCGACGACCTCGCCGCCGGCCGCTCCTCCCCTCCTCCGAGCCTCCAAGAAGCGGAAGTTTGGACCATCTGCCGAATCTTCCAGAGGAACATCACCCACAAGAAGCAGCCGCAGCCGCAGCTCGCCGTCGCCGCCGCCGCGGTGCCGGCGCCGGTGCCGGACGCGACGAGCTCGATCACCGGCAGCCTCGAGTCCGACAGCGCCGGCGACGACGTCGTCGAGTACATGAACACCCTCCAGCCGCCGCCGGCGAGCAACGTGAACGGTGGCTACAGCAACCAGCGCTACTTCCAGGAGCAATGGAACAGTAGCAGCAATGATAATACCACAGTGTTTCATCAGCATGCGGCGGCGGCGCCACCGCCGGAGCCTTCGCCGGCGACGGCGATGGCCGGATTCGGCCATGATCAGAGCGTTCTCAGCTCGCCGGCGCCGAGCGATTTCTACTACAAGGATGGATGCAACGATGACATTTACAGGATGGTGATGGAGCTCGCCGATCCGAGCTTGTTCTATGATCATATATATGCTTAA
CDS Sequence
- >LOC_Os07g04560.1
ATGAAGAGGGGTTGTGAAGATGAACTTGGAGCTGGTGATGTGATTCTACGAGGGGTGGAGGAGGTAGAAGAGGAAGATGATGATGACCTGGTTCTCCCCGGATTCCGGTTCCATCCTACCGACGAGGAGCTAGTGACGTTCTACCTTCGCCGGAAGATCGCCGAGAAGCGGCTGAGCATCGAGATCATCAAGGAGATGGACATCTACAAGCATGATCCCTCCGACTTCCTCAAGACGAGCACGGTGGGCAGCGAGAAGGAATGGTACTTCTTCTGCCTTCGAGGGAGGAAGTACAGGAACAGCATCCGACCCAACCGGGTCACCGGCTCCGGCTTCTGGAAGGCCACCGGAATCGACCGCCCAATCTGCTCTGCCGCCGGCGGCGGCGGCGGCGACTGCATCGGCCTCAAGAAGTCGCTCGTCTACTACCGCGGCAGCGCCGGCAAGGGCACCAAGACCGACTGGATGATGCACGAGTTCCGCCTCCCTCCGCCGCCCGCCGACGACCTCGCCGCCGGCCGCTCCTCCCCTCCTCCGAGCCTCCAAGAAGCGGAAGTTTGGACCATCTGCCGAATCTTCCAGAGGAACATCACCCACAAGAAGCAGCCGCAGCCGCAGCTCGCCGTCGCCGCCGCCGCGGTGCCGGCGCCGGTGCCGGACGCGACGAGCTCGATCACCGGCAGCCTCGAGTCCGACAGCGCCGGCGACGACGTCGTCGAGTACATGAACACCCTCCAGCCGCCGCCGGCGAGCAACGTGAACGGTGGCTACAGCAACCAGCGCTACTTCCAGGAGCAATGGAACAGTAGCAGCAATGATAATACCACAGTGTTTCATCAGCATGCGGCGGCGGCGCCACCGCCGGAGCCTTCGCCGGCGACGGCGATGGCCGGATTCGGCCATGATCAGAGCGTTCTCAGCTCGCCGGCGCCGAGCGATTTCTACTACAAGGATGGATGCAACGATGACATTTACAGGATGGTGATGGAGCTCGCCGATCCGAGCTTGTTCTATGATCATATATATGCTTAA
Protein Sequence
- >LOC_Os07g04560.1
MKRGCEDELGAGDVILRGVEEVEEEDDDDLVLPGFRFHPTDEELVTFYLRRKIAEKRLSIEIIKEMDIYKHDPSDFLKTSTVGSEKEWYFFCLRGRKYRNSIRPNRVTGSGFWKATGIDRPICSAAGGGGGDCIGLKKSLVYYRGSAGKGTKTDWMMHEFRLPPPPADDLAAGRSSPPPSLQEAEVWTICRIFQRNITHKKQPQPQLAVAAAAVPAPVPDATSSITGSLESDSAGDDVVEYMNTLQPPPASNVNGGYSNQRYFQEQWNSSSNDNTTVFHQHAAAAPPPEPSPATAMAGFGHDQSVLSSPAPSDFYYKDGCNDDIYRMVMELADPSLFYDHIYA*