Gene Details:

Functional Descriptions:

  • Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development, Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology.
  • Overexpression of SUI1 and SUI2 caused outgrowths of internodes during vegetative development, and we showed that expression patterns of Oryza Sativa Homeobox 15 (OSH15) and Histone4 were impaired.
  • We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages.
  • Whereas the expression of OSH1 continued within the shoot apical meristem, OSH15 expression within the shoot apical meristem ceased but became observable in a ring shaped pattern at the boundaries of some embryonic organs.
  • RNA in situ localization data suggest that OSH15 may play roles in the shoot organization during early embryogenesis and thereafter, OSH15 may be involved in morphogenetic events around the shoot apical meristem.
  • Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants.
  • Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching.
  • In transgenic plants carrying the OSH15 promoter-GUS reporter construct, the reporter gene was preferentially expressed in the AZ during young spikelet development.
  • The RNA in situ hybridization experiment also showed that OSH15 mRNAs were abundant in the AZ during spikelet development.
  • KNOX Protein OSH15 Induces Grain Shattering by Repressing Lignin Biosynthesis Genes.
  • Knockdown mutations of OSH15 showed reduced seed shattering phenotypes.
  • Analyses of OSH15 SH5-D double mutants showed that SH5 could not increase the degree of seed shattering when OSH15 was absent, indicating that SH5 functions together with OSH15.
  • In addition to the seed shattering phenotype, OSH15 mutants displayed dwarfism and accumulated a higher amount of lignin in internodes due to increased expression of the genes involved in lignin biosynthesis.
  • We conclude that OSH15 and SH5 form a dimer that enhances seed shattering by directly inhibiting lignin biosynthesis genes.
  • Here, we report the map-based cloning of RLB (RICE LATERAL BRANCH) encoding a nuclear-localized, KNOX-type homeobox protein from a rice cytokinin-deficient mutant showing more tillers, sparser panicles, defected floret morphology as well as attenuated shoot regeneration from callus.
  • RLB directly binds to the promoter and represses the transcription of OsCKX4, a cytokinin oxidase gene with high abundance in panicle branch meristem.
  • We proposed that RLB recruits PRC2 to the OsCKX4 promoter to epigenetically repress its transcription, which suppresses the catabolism of cytokinin, thereby promoting rice lateral branching.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os07g03770.1
    GTAGTATACACCTCCACCTTTCCCTTCCATTAATACCTCCACCCCCCACTCCTCTCCCCCATCTCCCCTCCCTCTCGCCATTGGAGCTAGACAGCTCGAGCTCCAGGAGGAAGAAGAGAGAGAGCCTAGCTGCTAGGGTTTCCATCGGATTTGGTTTTTTATTTTCTTTTTGTTTCTTGTGTGTGTTTTGATGGATCAGAGCTTTGGGAATCTTGGAGGAGGAGGAGGAGCAGGGGGGAGCGGCAAGGCGGCGGCGTCGTCGTTCCTGCAGCTGCCGCTGTCCACGGCGGCGGCGGCCACCGCGTACTACGGCACGCCGCTCGCCTTGCACCAGGCGGCGGCCGCGGCTGGCCCGTCGCAGTACCACGGTCACGGTCACCCCCACCACGGCGGCGGCCACCACCACAGCAAGCACGGCGGCGCCGGTGGTGGGGAGATCTCGGCGGCGGAGGCCGAGTCCATCAAGGCCAAGATCATGGCGCACCCCCAGTACTCCGCCCTCCTCGCAGCCTACCTCGACTGCCAGAAAGTCGGAGCGCCGCCGGAGGTGCTGGAGAGGCTGACCGCCACGGCGGCAAAGCTGGACGCCCGCCCTCCCGGCCGCCACGACGCGCGCGACCCGGAGCTCGACCAGTTCATGGAGGCGTACTGCAACATGCTGGCCAAGTACAGGGAGGAGCTGACGCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGAGGGTGGAGTCGCAGCTCGACACCATCGCCGGCGGCGCCCATGGCGGCGGCGCCGGCTCGGCGCGCCTCCTCCTCGCCGATGGTAAATCTGAATGTGTTGGTTCTTCTGAGGATGACATGGACCCAAGTGGCCGCGAAAACGAGCCGCCTGAGATCGACCCGCGCGCTGAGGATAAGGAGCTCAAGTTTCAGCTTCTGAAGAAGTACAGTGGCTACTTGAGCAGCCTAAGGCAAGAATTTTCCAAGAAAAAGAAGAAAGGAAAGCTGCCTAAGGAGGCCAGGCAGAAGCTGCTTCACTGGTGGGAGCTGCACTACAAGTGGCCTTACCCCTCAGAGACGGAGAAGATTGCGCTTGCGGAATCGACAGGACTAGATCAGAAGCAGATCAACAACTGGTTCATCAACCAGAGGAAACGGCACTGGAAGCCATCGGAGGACATGCCGTTCGTCATGATGGAAGGTTTTCACCCACAGAATGCTGCTGCATTGTACATGGATGGCCCGTTCATGGCAGATGGAATGTACCGCCTCGGTTCGTGAACCTCGATCTCGATCATCGGCGTGTTTGATGAGAGATCCAATGCCAAGATAAATTGATCATGGAATGTATTCAGCATGCGTTGCAATGCATGGACATTGTTATGGAATTTTTGGTTTATTTACCTTTCACCGTGGATTGACAAGGTCTCGATCATGTTAGTGTTGATGGCTTATAGTTCTCCAGTAATGTTGTTGTTTTTCCTTTCGATGGCTTGTAAAAGTTTAGGTGTATCGGAATTTCGATCAACTTGCTCGTACGCTGGTAATTAATTTGGTGATGGTCTATATGTTGTATGGTTGTGCGTTTCAGATTGGTGTTCAAAGTTGCCTATCTGAAACAATTATATATATTTATATTGCTTCTCATTTT
CDS Sequence
  • >LOC_Os07g03770.1
    ATGGATCAGAGCTTTGGGAATCTTGGAGGAGGAGGAGGAGCAGGGGGGAGCGGCAAGGCGGCGGCGTCGTCGTTCCTGCAGCTGCCGCTGTCCACGGCGGCGGCGGCCACCGCGTACTACGGCACGCCGCTCGCCTTGCACCAGGCGGCGGCCGCGGCTGGCCCGTCGCAGTACCACGGTCACGGTCACCCCCACCACGGCGGCGGCCACCACCACAGCAAGCACGGCGGCGCCGGTGGTGGGGAGATCTCGGCGGCGGAGGCCGAGTCCATCAAGGCCAAGATCATGGCGCACCCCCAGTACTCCGCCCTCCTCGCAGCCTACCTCGACTGCCAGAAAGTCGGAGCGCCGCCGGAGGTGCTGGAGAGGCTGACCGCCACGGCGGCAAAGCTGGACGCCCGCCCTCCCGGCCGCCACGACGCGCGCGACCCGGAGCTCGACCAGTTCATGGAGGCGTACTGCAACATGCTGGCCAAGTACAGGGAGGAGCTGACGCGGCCGATCGACGAGGCCATGGAGTTCCTCAAGAGGGTGGAGTCGCAGCTCGACACCATCGCCGGCGGCGCCCATGGCGGCGGCGCCGGCTCGGCGCGCCTCCTCCTCGCCGATGGTAAATCTGAATGTGTTGGTTCTTCTGAGGATGACATGGACCCAAGTGGCCGCGAAAACGAGCCGCCTGAGATCGACCCGCGCGCTGAGGATAAGGAGCTCAAGTTTCAGCTTCTGAAGAAGTACAGTGGCTACTTGAGCAGCCTAAGGCAAGAATTTTCCAAGAAAAAGAAGAAAGGAAAGCTGCCTAAGGAGGCCAGGCAGAAGCTGCTTCACTGGTGGGAGCTGCACTACAAGTGGCCTTACCCCTCAGAGACGGAGAAGATTGCGCTTGCGGAATCGACAGGACTAGATCAGAAGCAGATCAACAACTGGTTCATCAACCAGAGGAAACGGCACTGGAAGCCATCGGAGGACATGCCGTTCGTCATGATGGAAGGTTTTCACCCACAGAATGCTGCTGCATTGTACATGGATGGCCCGTTCATGGCAGATGGAATGTACCGCCTCGGTTCGTGA
Protein Sequence
  • >LOC_Os07g03770.1
    MDQSFGNLGGGGGAGGSGKAAASSFLQLPLSTAAAATAYYGTPLALHQAAAAAGPSQYHGHGHPHHGGGHHHSKHGGAGGGEISAAEAESIKAKIMAHPQYSALLAAYLDCQKVGAPPEVLERLTATAAKLDARPPGRHDARDPELDQFMEAYCNMLAKYREELTRPIDEAMEFLKRVESQLDTIAGGAHGGGAGSARLLLADGKSECVGSSEDDMDPSGRENEPPEIDPRAEDKELKFQLLKKYSGYLSSLRQEFSKKKKKGKLPKEARQKLLHWWELHYKWPYPSETEKIALAESTGLDQKQINNWFINQRKRHWKPSEDMPFVMMEGFHPQNAAALYMDGPFMADGMYRLGS*