Gene Details:

Functional Descriptions:

  • In this study, we characterized a rice mutant, termed crown rootless5 (Crl5), which produced fewer crown roots and displayed impaired initiation of crown root primordia.
  • The expression of Crl5, which encodes a member of the large AP2/ERF transcription factor family protein, was observed in the stem region where crown root initiation occurs.
  • A putative auxin response element in the Crl5 promoter region specifically interacted with a rice ARF, demonstrating that Crl5 may be a direct target of an ARF, similar to CRL1/ADVENTITIOUS ROOTLESS1 (ARL1) that also regulates crown root initiation.
  • A crl1 Crl5 double mutant displayed an additive phenotype, indicating that these two genes function in different genetic pathways for crown root initiation.
  • In addition, ProACT:Crl5/WT showed a cytokinin-resistant phenotype for crown root initiation, and also up-regulated the expression of two negative regulators of cytokinin signaling, OsRR1 and OsRR2, which were downregulated in Crl5.
  • Transgenic plants that over-expressed OsRR1 under the control of the Crl5 promoter in a Crl5 mutant background produced a higher number of crown roots than the Crl5 plant.
  • Taken together, these results indicate that auxin-induced Crl5 promotes crown root initiation through repression of cytokinin signaling by positively regulating type-A RR, OsRR1.
  • We have demonstrated that auxin-induced CROWN ROOTLESS5 (Crl5) regulates crown root initiation in rice through the induction of OsRR1, a negative regulator of cytokinin signaling.
  • A radicle initiated normally in a Crl5 mutant, in spite of the defect in crown root initiation, whereas crown roots, but not a radicle, were produced in a radicleless1 (ral1) mutant.
  • Exogenous auxin treatment induced Crl5 expression without de novo protein biosynthesis, which also required the degradation of AUX/IAA proteins.
  • Crl5 overexpressing calli formed adventitious roots, although Crl5 overexpressing plants did not induce ectopic roots, suggesting that Crl5, which promotes de novo root initiation, might function only in de-differentiated cells.
  • A Crl5 ral1 double mutant displayed an additive phenotype, showing that the formation of each root is regulated by different genetic mechanisms in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os07g03250.1
    AGAAGGAAGGCCAGACCAGGAAAGGGAGGCAAGGTGATTAGGTGGGTATGACATGGAGGAGAAAGCGGCGAGAGCGTATGACCTGGCGGCGCTCAAGTATTGGGGCCCTTCCACACACATCAACTTCCCGCTGGAGGACTACCAGGAGGAGCTGGAGGAGATGAAGAACATGACAAGGCAGGAGTATGTGGCTCACTTAAGGAGGAAAAGTAGCGGTTTCTCACGGGGAGCTTCCATGTACCGTGGTGTCACAAGGCATCATCAGCACGGACGGTGGCAGGCACGCATCGGCCGAGTCTCCGGCAACAAGGACCTCTACCTCGGCACTTTCAGCACGCAGGAGGAGGCGGCGGAGGCGTACGACGTGGCGGCGATCAAGTTCCGGGGGCTCAACGCCGTCACCAACTTCGACATCACCCGCTACGACGTCGACAAGATCATGGCCAGCAACACCCTCCTCCCCGCCGACCTCGCCCGCCGCAACGCCGCCACCACCACCTCCAAGGACGACCACTCCGCCGCCGGCGCCGGCGCCATTGTCTCGGTGCACTCCGCCGCCGACATCGCCGTCGCCGACACCCTCTGGAAGGCCACCACTGCCCCGAGGCAGCAGCAGCAGCACCACGACGACGTCGTGCTGTCCGGCGCCGACCAGGCCGCATTCTCCGTCCTCCACGACCTCGTCGCCGTCGACGCCGCGGCGGCGCACCAGCAGCAGCAGCAGCAGCAGCACATGTCCATGTCGGCGGCGTCGTCGCTGGTGACGAGCCTGAGCAACTCGCGCGAGGGGAGCCCCGACCGCGGCGGCGGCCTCTCCATGCTCTTCGCCAAGCCGTCGCCCGCCGTGGCGGCGTCGGCGCAGCAGCAGGCGTCGACCAAGCTCATGGCGGCGCCGCTGCCGCTCGGCTCCTGGGTCTCCTCGCCGCCGGCGTCTGCCAGGCCGCCCGCCGTCTCCATCGCCCACATGCCCCTCTTCGCCGCCTGGACCGACGCCTAATCACCCTCGCCGCCATGGCTCACCGGAGTAGTAATTAGCTAGCTATAGCTAGAGCTAGGCAACGACGACGACGACACTAGCTTAATTAGCTCGTGTTTAGTTGGTTAATTTAGGGTTCTTGGTTGCTCGGCGACATGATTAGCTAAGCTAGCGATGTAGTAGTAGCACTAGCAGCTAGTTGGAGTATTAATTAGTATCACTCAAGGCGAGGAAACCGACGCACGCCATGGCCGCGGCCTCTCCCCCACCTTTCCTTCTTCTCCGGCGACGGCCGGCCGCCATGTATCAGAGAGCTCGATCGGCGCCGGAGAAGAAGGGTTTTTGTAACACGGAGGCCGGAGATCTATCGAGGCGGTGCTTCTCCTCCAAAATCCAAAACTATATATCTTATATTAATTAATTAATTAATTATAGCCTCACCAAATTAATTTCTCAA
CDS Sequence
  • >LOC_Os07g03250.1
    ATGGAGGAGAAAGCGGCGAGAGCGTATGACCTGGCGGCGCTCAAGTATTGGGGCCCTTCCACACACATCAACTTCCCGCTGGAGGACTACCAGGAGGAGCTGGAGGAGATGAAGAACATGACAAGGCAGGAGTATGTGGCTCACTTAAGGAGGAAAAGTAGCGGTTTCTCACGGGGAGCTTCCATGTACCGTGGTGTCACAAGGCATCATCAGCACGGACGGTGGCAGGCACGCATCGGCCGAGTCTCCGGCAACAAGGACCTCTACCTCGGCACTTTCAGCACGCAGGAGGAGGCGGCGGAGGCGTACGACGTGGCGGCGATCAAGTTCCGGGGGCTCAACGCCGTCACCAACTTCGACATCACCCGCTACGACGTCGACAAGATCATGGCCAGCAACACCCTCCTCCCCGCCGACCTCGCCCGCCGCAACGCCGCCACCACCACCTCCAAGGACGACCACTCCGCCGCCGGCGCCGGCGCCATTGTCTCGGTGCACTCCGCCGCCGACATCGCCGTCGCCGACACCCTCTGGAAGGCCACCACTGCCCCGAGGCAGCAGCAGCAGCACCACGACGACGTCGTGCTGTCCGGCGCCGACCAGGCCGCATTCTCCGTCCTCCACGACCTCGTCGCCGTCGACGCCGCGGCGGCGCACCAGCAGCAGCAGCAGCAGCAGCACATGTCCATGTCGGCGGCGTCGTCGCTGGTGACGAGCCTGAGCAACTCGCGCGAGGGGAGCCCCGACCGCGGCGGCGGCCTCTCCATGCTCTTCGCCAAGCCGTCGCCCGCCGTGGCGGCGTCGGCGCAGCAGCAGGCGTCGACCAAGCTCATGGCGGCGCCGCTGCCGCTCGGCTCCTGGGTCTCCTCGCCGCCGGCGTCTGCCAGGCCGCCCGCCGTCTCCATCGCCCACATGCCCCTCTTCGCCGCCTGGACCGACGCCTAA
Protein Sequence
  • >LOC_Os07g03250.1
    MEEKAARAYDLAALKYWGPSTHINFPLEDYQEELEEMKNMTRQEYVAHLRRKSSGFSRGASMYRGVTRHHQHGRWQARIGRVSGNKDLYLGTFSTQEEAAEAYDVAAIKFRGLNAVTNFDITRYDVDKIMASNTLLPADLARRNAATTTSKDDHSAAGAGAIVSVHSAADIAVADTLWKATTAPRQQQQHHDDVVLSGADQAAFSVLHDLVAVDAAAAHQQQQQQQHMSMSAASSLVTSLSNSREGSPDRGGGLSMLFAKPSPAVAASAQQQASTKLMAAPLPLGSWVSSPPASARPPAVSIAHMPLFAAWTDA*