Gene Details:

• MSU gene ID: LOC_Os06g51260
• RAPdb gene ID: Os06g0728700
• Gene Symbol: OsLHY
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• OsCCA1 also regulates IPA1 expression to mediate panicle and grain development.
• Downregulating and overexpressing OsCCA1 increases and reduces tiller numbers, respectively, whereas manipulating PSEUDORESPONSE REGULATOR1 (OsPPR1) expression results in the opposite effects.
• Genetic analyses using double mutants and overexpression in the mutants show that OsTB1, D14, and IPA1 act downstream of OsCCA1 Sugars repress OsCCA1 expression in roots and tiller buds to promote tiller-bud outgrowth.
• Additionally, the dual function of OsLHY entirely relied on Hd1, as the double mutant OsLHY hd1 showed the same heading date with hd1 under about 11.
• Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice.
• By MutMap technique, we identified the candidate gene OsLHY from a later flowering mutant lem1 and further confirmed it through genetic complementation, RNA interference knockdown, and CRISPR/Cas9-knockout.
• Here we showed that CRISPR/Cas9-engineered mutations in OsLHY caused late flowering in rice only under natural long-day (nLD) and short-day (nSD) conditions, but not artificial SD (10 h light/14 h dark) conditions.
• Furthermore, the expression of the flowering activators Ehd1, Hd3a and RFT1 was down-regulated and flowering repressors Hd1 and Ghd7 was up-regulated in the OsLHY mutant under LD conditions.
• These results suggested that the OsLHY can promote rice flowering mainly through regulating Hd1 and Ehd1.
• SDG724 encodes a histone H3K36 methyltransferase, and OsLHY is a vital circadian rhythm transcription factor.
• Previously, we reported that SDG724 and OsLHY are two rice heading date regulators in rice.

Function-related keywords:

• development ,  panicle ,  grain ,  tiller ,  tiller-number ,  heading-date ,  flowering ,  transcription-factor

Literature:

• The evolutionarily conserved OsPRR quintet: rice pseudo-response regulators implicated in circadian rhythm .  DOI:  10.1093/pcp/pcg135 ;  PMID:  14634161
• The role of casein kinase II in flowering time regulation has diversified during evolution .  DOI:  10.1104/pp.109.148908 ;  PMID:  20007447
• Nitrogen Mediates Flowering Time and Nitrogen Use Efficiency via Floral Regulators in Rice .  DOI:  10.1016/j.cub.2020.10.095 ;  PMID:  33278354
• Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis .  DOI:  10.15252/embj.2020105086 ;  PMID:  33347628
• Transcriptional and post-transcriptional regulation of heading date in rice .  DOI:  10.1111/nph.17158 ;  PMID:  33341945
• The transcriptional repressor OsPRR73 links circadian clock and photoperiod pathway to control heading date in rice .  DOI:  10.1111/pce.13987 ;  PMID:  33377200
• Dual function of clock component OsLHY sets critical day length for photoperiodic flowering in rice .  DOI:  10.1111/pbi.13580 ;  PMID:  33740293
• OsLHY is involved in regulating flowering through the Hd1- and Ehd1- mediated pathways in rice (Oryza sativa L.) .  DOI:  10.1016/j.plantsci.2021.111145 ;  PMID:  35067308
• Crosstalk between the Circadian Clock and Histone Methylation .  DOI:  10.3390/ijms23126465 ;  PMID:  35742907
• Bifunctional regulators of photoperiodic flowering in short day plant rice .  DOI:  10.3389/fpls.2022.1044790 ;  PMID:  36340409

Related News:

Gene Resources:

• NCBI ID:
• UniProt accessions:

Sequences: