Gene Details:

• MSU gene ID: LOC_Os06g51084
• RAPdb gene ID: Os06g0726400
• Gene Symbol: BEI SBE1
• Genome: MSU7 ,  IRGSP-1.0
• Species: Oryza sativa

Functional Descriptions:

• Rice starch branching enzyme 1 (SBE1) and granule-bound starch synthase (GBSS) catalyzed the biosynthesis of amylopectin and amylose in rice developing endosperm respectively, and the genes encoding these two enzymes, SBE1 and waxy, are mainly expressed in the endosperm.
• The cDNA of two SBE(starch-branching enzyme) genes SBE1 and Sbe3 encoding SBE I and SBE III (two major isoforms in rice) were cloned by an improved RT-PCR technique, from a template cDNA library derived from the total mRNAs extracted from the immature seeds of a japonica rice Wuyunjing 7.
• The cloned SBE1 and Sbe3 cDNAs make it possible to improve rice starch quality through genetic engineering.
• We determined the crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose at a resolution of 2.
• Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose.
• We overproduced rice branching enzyme I (BEI) in Escherichia coli cells, and the resulting enzyme (rBEI) was characterized with respect to biochemical and crystallographic properties.
• Biochemical and Crystallographic Characterization of the Starch Branching Enzyme I (BEI) fromOryza sativaL.
• The rice branching enzyme I (BEI) overproduced in Escherichia coli cells was investigated with respect to action on starches.
• The Action of Rice Branching Enzyme I (BEI) on Starches.
• Within the 5’-upstream region of rice SBE1 gene, we identified a 53 bp fragment C53 which could interact with the nuclear proteins extracted from rice endosperm.
• These results suggested that the expression of SBE1 and waxy gene in rice developing endosperm may be coordinately regulated by REB-like bZIP transcriptional factors.
• Furthermore, OsbZIP58 was shown to bind directly to the promoters of six starch-synthesizing genes, OsAGPL3, Wx, OsSSIIa, SBE1, OsBEIIb, and ISA2, and to regulate their expression.
• BEI treatment decreased the turbidity of starch suspensions with distinct pasting behaviors from a native starch.
• Mutations of either BEI or BEIIa did not alter the starch crystallite pattern (A-type).
• It is concluded that the relative importance in determining starch fine structures and functionality was in the order of BEIIb > BEI > BEIIa.

Function-related keywords:

• starch ,  seed ,  branching ,  endosperm

Literature:

• Crystal structure of the rice branching enzyme I (BEI) in complex with maltopentaose .  DOI:  10.1016/j.bbrc.2012.06.145 ;  PMID:  22771800
• Interaction of rice bZIP protein REB with the 5′-upstream region of both rice sbe1 gene and waxy gene .  DOI:  10.1360/02tb9074
• Biochemical and crystallographic characterization of the starch branching enzyme I (BEI) from Oryza sativa L .  DOI:  10.1271/bbb.80325 ;  PMID:  18997409
• The action of rice branching enzyme I (BEI) on starches .  DOI:  10.1271/bbb.90352 ;  PMID:  19897896
• cDNA Cloning and Sequence Analysis of Rice Sbe1 and Sbe3 Genes .
• OsbZIP58, a basic leucine zipper transcription factor, regulates starch biosynthesis in rice endosperm .  DOI:  10.1093/jxb/ert187 ;  PMID:  23846875
• Relative importance of branching enzyme isoforms in determining starch fine structure and physicochemical properties of indica rice .  DOI:  10.1007/s11103-021-01207-y ;  PMID:  34750721

Related News:

Gene Resources:

• NCBI ID: D11082
• UniProt accessions:

Sequences: