Gene Details:

Functional Descriptions:

  • The sl1 mutant largely resembles the rice B-class gene mutant SPW1; both exhibit homeotic conversions of lodicules and stamens to palea/lemma-like organs and carpels.
  • Transgenic lines from the introduced gene expressing double-stranded RNA with the OsMADS16 cDNA fragment were male-sterile and displayed alternations of lodicules and stamens, occasionally depressed palea and overgrown glume.
  • Two SPW1 alleles, SPW1-1 and SPW1-2, show the same floral phenotype and did not affect vegetative development.
  • The homeotic mutation SPW1 transforms stamens and lodicules into carpels and palea-like organs, respectively.
  • Based on genetic and molecular results, we postulate a model of stamen and carpel specification in rice, with DL as a novel gene controlling carpel identity and acting mutually and antagonistically to the class B gene, SPW1.
  • Alteration of floral organ identity in rice through ectopic expression of OsMADS16.
  • Our results demonstrate that OsMADS16 is an AP3/DEF orthologue to specify the identities of lodicules and stamens in rice flower and also support that OsMADS4 is a PI orthologue.
  • We also show that SL1 specifies lodicule and stamen identities through positive transcriptional regulation of SPW1/OsMADS16 expression.
  • The flower of the double mutant, SPW1 dl-sup, produces incompletely differentiated organs indefinitely after palea-like organs are produced in the position where lodicules are formed in the wild-type flower.
  • These results indicate that expression of OsMADS16 in the innermost whorl induces stamen development.
  • Consistent with the mutant floral phenotype, DDF1 positively regulates B-class genes OsMADS4 and OsMADS16, and negatively regulates pistil specification gene DL.
  • We identified a cleistogamous mutant of rice harbouring a missense mutation (the 45th residue isoleucine to threonine; I45T) in the class-B MADS-box gene SUPERWOMAN1 (SPW1), which specifies the identities of lodicules (equivalent to petals) and stamens.
  • In the mutant, SPW1-cls, the stamens are normal, but the lodicules are transformed homeotically to lodicule-glume mosaic organs, thereby engendering cleistogamy.
  • We analyzed recessive mutants of two homeotic genes in rice, SUPERWOMAN1 (SPW1) and DROOPING LEAF (DL).
  • To investigate if OsMADS16 functions as an AP3/DEF orthologue to regulate the development of lodicules and stamens in rice, we isolated its genomic sequences and characterized its functions in planta by RNA interference.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g49840.2
    AATACTGCAGGATCCGTCTCCGCGAACCGAGAGACGTTACGGTATACGGAGTGGGCACGATTGCTTTCACGAGTTGGGAATCCGTTGCTGTCGTACGTCTCGTCTATGGCGTGCTTAGCTAGCTAGCTAGCTAGCTAGCTGCATTGTCCGGCGAGAGAGATAGCTGCTGCAGGGGGCGGCCATGGGGAGGGGCAAGATCGAGATCAAGCGGATCGAGAACGCGACCAACAGGCAGGTGACCTACTCGAAGCGCCGCACGGGGATCATGAAGAAGGCCAGGGAGCTCACCGTGCTCTGCGACGCCCAGGTCGCCATCATCATGTTCTCCTCCACCGGCAAGTACCACGAGTTCTGCAGCCCTTCCACCGACATCAAGGGGATCTTTGACCGCTACCAGCAAGCCATCGGCACCAGCCTTTGGATCGAGCAGTATGAGAATATGCAGCGCACGCTGAGCCATCTCAAGGACATCAACCGCAACCTGCGCACCGAGATCAGGCAAAGGATGGGAGAAGATCTGGACGGGCTGGAGTTCGACGAGCTGCGCGGTCTTGAGCAAAATGTCGATGCCGCCCTCAAGGAGGTTCGCCACAGGAAGTATCATGTGATCACCACACAGACTGAAACCTACAAGAAAAAGGTGAAGCACTCCTACGAGGCGTACGAGACTCTGCAGCAGGAGCTGGGGTTGCGCGAGGAGCCGGCGTTCGGGTTCGTGGACAACACCGGCGGCGGGTGGGACGGCGGCGCCGGCGCCGGCGCGGCGGCGGACATGTTCGCCTTCCGCGTGGTGCCCAGCCAGCCCAACCTGCACGGCATGGCCTACGGCGGCAACCACGACCTGCGCCTCGGTTGATATATCATCGCCGCCGCCGCCGCCGCCGCCGGCGGCCTGCTCGTCGCCGGCCGGCCGGCCGCCGGACACCGTATCATCGCTCGATCTAGATAACAAGCCGACGAATAAATTGCAGTGCTCGTGTTACTTACCTGCATGCGCATGCATTATTATCGATCAACGCAAGAACTTCATTTCATTTACCAAACCACTTGATGTGTGCTATGATCCAGCGGTTAAACCGTGTGTTGTCTCTTGACATTTTGTACCAGTTACTGATGATGTTGTATTATGTATGCGTGGTGTGCAACTGTGCTTGGATAATTTCACACTTATTTGCCACGCTTGCTTTATTAGCTTTGACTATCAAGCTACTGATCAGAGAGTTACCTAGCTCAAAGGATTAATTATTATGTATACAGCTCAAATACATTAATGGGCTACTGGCTGCTGCTGTCCGAGTAATTATTTACACCTGTGG
  • >LOC_Os06g49840.1
    AATACTGCAGGATCCGTCTCCGCGAACCGAGAGACGTTACGGTATACGGAGTGGGCACGATTGCTTTCACGAGTTGGGAATCCGTTGCTGTCGTACGTCTCGTCTATGGCGTGCTTAGCTAGCTAGCTAGCTAGCTAGCTGCATTGTCCGGCGAGAGAGATAGCTGCTGCAGGGGGCGGCCATGGGGAGGGGCAAGATCGAGATCAAGCGGATCGAGAACGCGACCAACAGGCAGGTGACCTACTCGAAGCGCCGCACGGGGATCATGAAGAAGGCCAGGGAGCTCACCGTGCTCTGCGACGCCCAGGTCGCCATCATCATGTTCTCCTCCACCGGCAAGTACCACGAGTTCTGCAGCCCTTCCACCGACATCAAGGGGATCTTTGACCGCTACCAGCAAGCCATCGGCACCAGCCTTTGGATCGAGCAGTATGAGAATATGCAGCGCACGCTGAGCCATCTCAAGGACATCAACCGCAACCTGCGCACCGAGATCAGGCAAAGGATGGGAGAAGATCTGGACGGGCTGGAGTTCGACGAGCTGCGCGGTCTTGAGCAAAATGTCGATGCCGCCCTCAAGGAGGTTCGCCACAGGAAGTATCATGTGATCACCACACAGACTGAAACCTACAAGAAAAAGGTGAAGCACTCCTACGAGGCGTACGAGACTCTGCAGCAGGAGCTGGGGTTGCGCGAGGAGCCGGCGTTCGGGTTCGTGGACAACACCGGCGGCGGGTGGGACGGCGGCGCCGGCGCCGGCGCGGCGGCGGACATGTTCGCCTTCCGCGTGGTGCCCAGCCAGCCCAACCTGCACGGCATGGCCTACGGCGGCAACCACGACCTGCGCCTCGGTTGATATATCATCGCCGCCGCCGCCGCCGCCGCCGGCGGCCTGCTCGTCGCCGGCCGGCCGGCCGCCGGACACCGTATCATCGCTCGATCTAGATAACAAGCCGACGAATAAATTGCAGTGCTCGTGTTACTTACCTGCATGCGCATGCATTATTATCGATCAACGCAAGAACTTCATTTCATTTACCAAACCACTTGATGTGTGCTATGATCCAGCGGTTAAACCGTGTGTTGTCTCTTGACATTTTGTACCAGTTACTGATGATGTTGTATTATGTATGCGTGGTGTGCAACTGTGCTTGGATAATTTCACACTTATTTGCCACGCTTGCTTTATTAGCTTTGACTATCAAGCTACTGATCAGAGAGTTACCTAGCTCAAAGGATTAATTATTATGTATACAGCTCAAATACATTAATGGGCTACTGGCTGCTGCTGTCCGAGTAATTATTTACACCTGTGG
CDS Sequence
  • >LOC_Os06g49840.2
    ATGGGGAGGGGCAAGATCGAGATCAAGCGGATCGAGAACGCGACCAACAGGCAGGTGACCTACTCGAAGCGCCGCACGGGGATCATGAAGAAGGCCAGGGAGCTCACCGTGCTCTGCGACGCCCAGGTCGCCATCATCATGTTCTCCTCCACCGGCAAGTACCACGAGTTCTGCAGCCCTTCCACCGACATCAAGGGGATCTTTGACCGCTACCAGCAAGCCATCGGCACCAGCCTTTGGATCGAGCAGTATGAGAATATGCAGCGCACGCTGAGCCATCTCAAGGACATCAACCGCAACCTGCGCACCGAGATCAGGCAAAGGATGGGAGAAGATCTGGACGGGCTGGAGTTCGACGAGCTGCGCGGTCTTGAGCAAAATGTCGATGCCGCCCTCAAGGAGGTTCGCCACAGGAAGTATCATGTGATCACCACACAGACTGAAACCTACAAGAAAAAGGTGAAGCACTCCTACGAGGCGTACGAGACTCTGCAGCAGGAGCTGGGGTTGCGCGAGGAGCCGGCGTTCGGGTTCGTGGACAACACCGGCGGCGGGTGGGACGGCGGCGCCGGCGCCGGCGCGGCGGCGGACATGTTCGCCTTCCGCGTGGTGCCCAGCCAGCCCAACCTGCACGGCATGGCCTACGGCGGCAACCACGACCTGCGCCTCGGTTGA
  • >LOC_Os06g49840.1
    ATGGGGAGGGGCAAGATCGAGATCAAGCGGATCGAGAACGCGACCAACAGGCAGGTGACCTACTCGAAGCGCCGCACGGGGATCATGAAGAAGGCCAGGGAGCTCACCGTGCTCTGCGACGCCCAGGTCGCCATCATCATGTTCTCCTCCACCGGCAAGTACCACGAGTTCTGCAGCCCTTCCACCGACATCAAGGGGATCTTTGACCGCTACCAGCAAGCCATCGGCACCAGCCTTTGGATCGAGCAGTATGAGAATATGCAGCGCACGCTGAGCCATCTCAAGGACATCAACCGCAACCTGCGCACCGAGATCAGGCAAAGGATGGGAGAAGATCTGGACGGGCTGGAGTTCGACGAGCTGCGCGGTCTTGAGCAAAATGTCGATGCCGCCCTCAAGGAGGTTCGCCACAGGAAGTATCATGTGATCACCACACAGACTGAAACCTACAAGAAAAAGGTGAAGCACTCCTACGAGGCGTACGAGACTCTGCAGCAGGAGCTGGGGTTGCGCGAGGAGCCGGCGTTCGGGTTCGTGGACAACACCGGCGGCGGGTGGGACGGCGGCGCCGGCGCCGGCGCGGCGGCGGACATGTTCGCCTTCCGCGTGGTGCCCAGCCAGCCCAACCTGCACGGCATGGCCTACGGCGGCAACCACGACCTGCGCCTCGGTTGA
Protein Sequence
  • >LOC_Os06g49840.2
    MGRGKIEIKRIENATNRQVTYSKRRTGIMKKARELTVLCDAQVAIIMFSSTGKYHEFCSPSTDIKGIFDRYQQAIGTSLWIEQYENMQRTLSHLKDINRNLRTEIRQRMGEDLDGLEFDELRGLEQNVDAALKEVRHRKYHVITTQTETYKKKVKHSYEAYETLQQELGLREEPAFGFVDNTGGGWDGGAGAGAAADMFAFRVVPSQPNLHGMAYGGNHDLRLG*
  • >LOC_Os06g49840.1
    MGRGKIEIKRIENATNRQVTYSKRRTGIMKKARELTVLCDAQVAIIMFSSTGKYHEFCSPSTDIKGIFDRYQQAIGTSLWIEQYENMQRTLSHLKDINRNLRTEIRQRMGEDLDGLEFDELRGLEQNVDAALKEVRHRKYHVITTQTETYKKKVKHSYEAYETLQQELGLREEPAFGFVDNTGGGWDGGAGAGAAADMFAFRVVPSQPNLHGMAYGGNHDLRLG*