Gene Details:

Functional Descriptions:

  • Suppression of endogenous OsLIC expression resulted in drastically increased leaf and tiller angles, shortened shoot height, and consequently reduced grain production in rice.
  • Here, we demonstrate that Oryza sativa LEAF and TILLER ANGLE INCREASED CONTROLLER (LIC) acts as an antagonistic transcription factor of BRASSINAZOLE-RESISTANT 1 (BZR1) to attenuate the BR signaling pathway.
  • OsLIC, a Novel CCCH-Type Zinc Finger Protein with Transcription Activation, Mediates Rice Architecture via Brassinosteroids Signaling.
  • Quantitative PCR revealed LIC transcription rapidly induced by BR treatment.
  • Image analysis and immunoblotting showed that upon BR treatment LIC proteins translocate from the cytoplasm to the nucleus in a phosphorylation-dependent fashion.
  • Molecular and physiology assays revealed that LIC plays a dominant role at high BR levels, whereas BZR1 is dominant at low levels.
  • Here we describe a novel CCCH-type zinc finger gene, OsLIC (Oraza sativaleaf and tiller angle increased controller), which is involved in the regulation of rice plant architecture.
  • OsLIC may be a transcription activator to control rice plant architecture.
  • OsLIC is predominantly expressed in rice collar and tiller bud.
  • The gain-of-function mutant LIC-1 and LIC-overexpressing lines showed erect leaves, similar to BZR1-depleted lines, which indicates the opposite roles of LIC and BZR1 in regulating leaf bending.
  • LIC directly regulated target genes such as INCREASED LEAF INCLINATION 1 (ILI1) to oppose the action of BZR1.
  • Thus, LIC regulates rice leaf bending as an antagonistic transcription factor of BZR1.
  • Dynamics of brassinosteroid response modulated by negative regulator LIC in rice.
  • The phenotypes of LIC-1 and LIC-overexpressing lines in erect leaves contribute to ideal plant architecture.
  • OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice.
  • Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC.
  • OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak.
  • Rice OsLIC encoding a CCCH zinc finger transcription factor plays an important role in immunity.
  • Genetic assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc, knocking out OsWRKY30 in the OsLIC mutants attenuated the resistance against bacterial pathogens.
  • OsMAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity, therefore, overexpression of OsLIC partially suppressed OsMAPK6-mediated rice resistance.
  • Collectively, these results reveal that OsMAPK6-mediated phosphorylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription, suggesting that OsMAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g49080.1
    ACTACTCCGGCCGGCGGCCGCGTCTCGAGTTCTCGGCGGAGGCGGCAAGCCGGCGAGGGCAACCCACCCACCGACGTCGTGCGGCCGCGCTTGCTGCGTCGCGTGCCCACGCCAATCCCGTGGCGGCGGAAGGAGGAGGAGCAAGCGCTTGGGAGGAGCTAAACCCTAGAGGCGGAGCTCTAGAATCGAATCCCCCCCCTTCCTTCCGCTTTGCGGCGATGAGTCGGCGGCAGGAGATTTGCCGCAACTTCCAGCGCGGAAGTTGCAAGTACGGAGCGCAGTGCAGGTATTTGCATGCCTCCCCTCACCAGCAACAGCAGCAGCAGCAGGCGAAGCCCAACCCGTTTGGATTTGGCACCGGAAGCAGGCAGCAGCAGCAGCCGTCATTTGGCTCACAGTTCCAGCAGCAGCAACAGCAGCAGCAGAAGCCCAATCCTTTTGGTTTTGGGGTACAAGGTGCCAATGCCCAGTCACGTAATGCTCCTGGTCCTGCGAAGCCTTTTCAAAATAAGTGGGTAAGGGACCCCTCGGCCCCGACGAAGCAAACAGAGGCGGTGCAGCCACCGCAGGCGCAGGCTGCCCACACCTCCTGTGAAGACCCTCAGTCATGCAGACAACAAATTTCTGAGGATTTTAAGAACGAGGCTCCTATTTGGAAGCTTACTTGTTATGCTCATCTCAGAAATGGCCCTTGCAATATTAAGGGAGACATTAGCTTCGAGGAACTAAGAGCTAAAGCCTATGAGGAAGGAAAGCAAGGGCATTCTCTGCAATCAATAGTTGAGGGAGAAAGAAATCTACAAAACGCAAAGTTAATGGAGTTTACTAATCTGCTCAATAGTGCACGTCCATCACAAACTCCAAGCTTTCCTACTATGAGTTCCTTCCCTGAAGTGAAAAACAACTCATCATTCGGGGCTTCTCAAACCAATGGACCACCTGTGTTCAGTAGTTTCAGTCAAATTGGAGCAGCAACTAATATTGGACCTGGGCCAGGAACCACCGCACCAGGAATGCCAGCCAGTAGTCCTTTTGGTCATCCAAGCTCTGCACCACTTGCTGCCCCTACTTTTGGCAGTTCTCAAATGAAGTTTGGAGTTTCGAGCGTATTTGGAAACCAGGGTTCAGGTCAACCATTTGGGAGCTTTCAAGCCCCACGTTTTCCCAGTTCGAAGTCTCCTGCTTCCTCTGTCCAGCACAGAGACATTGATAGGCAATCACAAGAGTTGCTTAATGGGATGGTAACTCCTCCTAGTGTGATGTTTGAGGAATCTGTTGGGAACAACAAAAATGAAAATCAGGATGATAGCATCTGGTTGAAGGAAAAATGGGCAATTGGGGAGATACCGCTGGATGAACCACCTCAGAGGCACGTTAGCCATGTGTTTTAAGAACTCAAAGGTGTTTTTTTTTGTGTTGTTGAGGCATGAGACCATGAACTACTCAGCTATGATCTGACTTCCTTGTAGTTGGGTTGCCACCATATGTCGTACTTTTCTTGCCCGAGCTTCATGACGATACAAAGGAATTATACAACAAACAAGAGAATTTGCAAGAGTGAACAGTGAAGGAAGGCTGATGAACCAATGCATTGTTTGGTACCACTTATTTATTCCTGGAACATGAATGTGGGCAACTAGAGCTAACAACCAGCTAACTGCAGTCTTGCCTGTCGAGTTAGTATGTTTTTCTCTCTTTTTTTTAATCTGTAATTGTTTATTCCAATAGTATGTTTTTCTTTTTTTTTAATCTGTAATTGTTTATTCCAAAACTTGTTAGGAAGTTATTGGAATAAGAAACAGTATTGCAATATTGAGTGATTGGCCGGGTAACA
CDS Sequence
  • >LOC_Os06g49080.1
    ATGAGTCGGCGGCAGGAGATTTGCCGCAACTTCCAGCGCGGAAGTTGCAAGTACGGAGCGCAGTGCAGGTATTTGCATGCCTCCCCTCACCAGCAACAGCAGCAGCAGCAGGCGAAGCCCAACCCGTTTGGATTTGGCACCGGAAGCAGGCAGCAGCAGCAGCCGTCATTTGGCTCACAGTTCCAGCAGCAGCAACAGCAGCAGCAGAAGCCCAATCCTTTTGGTTTTGGGGTACAAGGTGCCAATGCCCAGTCACGTAATGCTCCTGGTCCTGCGAAGCCTTTTCAAAATAAGTGGGTAAGGGACCCCTCGGCCCCGACGAAGCAAACAGAGGCGGTGCAGCCACCGCAGGCGCAGGCTGCCCACACCTCCTGTGAAGACCCTCAGTCATGCAGACAACAAATTTCTGAGGATTTTAAGAACGAGGCTCCTATTTGGAAGCTTACTTGTTATGCTCATCTCAGAAATGGCCCTTGCAATATTAAGGGAGACATTAGCTTCGAGGAACTAAGAGCTAAAGCCTATGAGGAAGGAAAGCAAGGGCATTCTCTGCAATCAATAGTTGAGGGAGAAAGAAATCTACAAAACGCAAAGTTAATGGAGTTTACTAATCTGCTCAATAGTGCACGTCCATCACAAACTCCAAGCTTTCCTACTATGAGTTCCTTCCCTGAAGTGAAAAACAACTCATCATTCGGGGCTTCTCAAACCAATGGACCACCTGTGTTCAGTAGTTTCAGTCAAATTGGAGCAGCAACTAATATTGGACCTGGGCCAGGAACCACCGCACCAGGAATGCCAGCCAGTAGTCCTTTTGGTCATCCAAGCTCTGCACCACTTGCTGCCCCTACTTTTGGCAGTTCTCAAATGAAGTTTGGAGTTTCGAGCGTATTTGGAAACCAGGGTTCAGGTCAACCATTTGGGAGCTTTCAAGCCCCACGTTTTCCCAGTTCGAAGTCTCCTGCTTCCTCTGTCCAGCACAGAGACATTGATAGGCAATCACAAGAGTTGCTTAATGGGATGGTAACTCCTCCTAGTGTGATGTTTGAGGAATCTGTTGGGAACAACAAAAATGAAAATCAGGATGATAGCATCTGGTTGAAGGAAAAATGGGCAATTGGGGAGATACCGCTGGATGAACCACCTCAGAGGCACGTTAGCCATGTGTTTTAA
Protein Sequence
  • >LOC_Os06g49080.1
    MSRRQEICRNFQRGSCKYGAQCRYLHASPHQQQQQQQAKPNPFGFGTGSRQQQQPSFGSQFQQQQQQQQKPNPFGFGVQGANAQSRNAPGPAKPFQNKWVRDPSAPTKQTEAVQPPQAQAAHTSCEDPQSCRQQISEDFKNEAPIWKLTCYAHLRNGPCNIKGDISFEELRAKAYEEGKQGHSLQSIVEGERNLQNAKLMEFTNLLNSARPSQTPSFPTMSSFPEVKNNSSFGASQTNGPPVFSSFSQIGAATNIGPGPGTTAPGMPASSPFGHPSSAPLAAPTFGSSQMKFGVSSVFGNQGSGQPFGSFQAPRFPSSKSPASSVQHRDIDRQSQELLNGMVTPPSVMFEESVGNNKNENQDDSIWLKEKWAIGEIPLDEPPQRHVSHVF*