LIC;OsLIC1

Gene Details

MSU gene ID: LOC_Os06g49080
RAPdb gene ID: Os06g0704300
Gene Symbol: LIC OsLIC1 OsLIC OsFLA6
Genome: MSU7 , IRGSP-1.0
Species: Oryza sativa

Functional Descriptions

Suppression of endogenous OsLIC expression resulted in drastically increased leaf and tiller angles, shortened shoot height, and consequently reduced grain production in rice.
Here, we demonstrate that Oryza sativa LEAF and TILLER ANGLE INCREASED CONTROLLER (LIC) acts as an antagonistic transcription factor of BRASSINAZOLE-RESISTANT 1 (BZR1) to attenuate the BR signaling pathway.
OsLIC, a Novel CCCH-Type Zinc Finger Protein with Transcription Activation, Mediates Rice Architecture via Brassinosteroids Signaling.
Quantitative PCR revealed LIC transcription rapidly induced by BR treatment.
Image analysis and immunoblotting showed that upon BR treatment LIC proteins translocate from the cytoplasm to the nucleus in a phosphorylation-dependent fashion.
Molecular and physiology assays revealed that LIC plays a dominant role at high BR levels, whereas BZR1 is dominant at low levels.
Here we describe a novel CCCH-type zinc finger gene, OsLIC (Oraza sativaleaf and tiller angle increased controller), which is involved in the regulation of rice plant architecture.
OsLIC may be a transcription activator to control rice plant architecture.
OsLIC is predominantly expressed in rice collar and tiller bud.
The gain-of-function mutant LIC-1 and LIC-overexpressing lines showed erect leaves, similar to BZR1-depleted lines, which indicates the opposite roles of LIC and BZR1 in regulating leaf bending.
LIC directly regulated target genes such as INCREASED LEAF INCLINATION 1 (ILI1) to oppose the action of BZR1.
Thus, LIC regulates rice leaf bending as an antagonistic transcription factor of BZR1.
Dynamics of brassinosteroid response modulated by negative regulator LIC in rice.
The phenotypes of LIC-1 and LIC-overexpressing lines in erect leaves contribute to ideal plant architecture.
OsRELA Regulates Leaf Inclination by Repressing the Transcriptional Activity of OsLIC in Rice.
Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC.
OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak.
Rice OsLIC encoding a CCCH zinc finger transcription factor plays an important role in immunity.
Genetic assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc, knocking out OsWRKY30 in the OsLIC mutants attenuated the resistance against bacterial pathogens.
OsMAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity, therefore, overexpression of OsLIC partially suppressed OsMAPK6-mediated rice resistance.
Collectively, these results reveal that OsMAPK6-mediated phosphorylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription, suggesting that OsMAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens.

Functional Keywords

Literature and News

Gene Resources

Sequences

cDNA Sequence

>LOC_Os06g49080.1
ACTACTCCGGCCGGCGGCCGCGTCTCGAGTTCTCGGCGGAGGCGGCAAGCCGGCGAGGGCAACCCACCCACCGACGTCGTGCGGCCGCGCTTGCTGCGTCGCGTGCCCACGCCAATCCCGTGGCGGCGGAAGGAGGAGGAGCAAGCGCTTGGGAGGAGCTAAACCCTAGAGGCGGAGCTCTAGAATCGAATCCCCCCCCTTCCTTCCGCTTTGCGGCGATGAGTCGGCGGCAGGAGATTTGCCGCAACTTCCAGCGCGGAAGTTGCAAGTACGGAGCGCAGTGCAGGTATTTGCATGCCTCCCCTCACCAGCAACAGCAGCAGCAGCAGGCGAAGCCCAACCCGTTTGGATTTGGCACCGGAAGCAGGCAGCAGCAGCAGCCGTCATTTGGCTCACAGTTCCAGCAGCAGCAACAGCAGCAGCAGAAGCCCAATCCTTTTGGTTTTGGGGTACAAGGTGCCAATGCCCAGTCACGTAATGCTCCTGGTCCTGCGAAGCCTTTTCAAAATAAGTGGGTAAGGGACCCCTCGGCCCCGACGAAGCAAACAGAGGCGGTGCAGCCACCGCAGGCGCAGGCTGCCCACACCTCCTGTGAAGACCCTCAGTCATGCAGACAACAAATTTCTGAGGATTTTAAGAACGAGGCTCCTATTTGGAAGCTTACTTGTTATGCTCATCTCAGAAATGGCCCTTGCAATATTAAGGGAGACATTAGCTTCGAGGAACTAAGAGCTAAAGCCTATGAGGAAGGAAAGCAAGGGCATTCTCTGCAATCAATAGTTGAGGGAGAAAGAAATCTACAAAACGCAAAGTTAATGGAGTTTACTAATCTGCTCAATAGTGCACGTCCATCACAAACTCCAAGCTTTCCTACTATGAGTTCCTTCCCTGAAGTGAAAAACAACTCATCATTCGGGGCTTCTCAAACCAATGGACCACCTGTGTTCAGTAGTTTCAGTCAAATTGGAGCAGCAACTAATATTGGACCTGGGCCAGGAACCACCGCACCAGGAATGCCAGCCAGTAGTCCTTTTGGTCATCCAAGCTCTGCACCACTTGCTGCCCCTACTTTTGGCAGTTCTCAAATGAAGTTTGGAGTTTCGAGCGTATTTGGAAACCAGGGTTCAGGTCAACCATTTGGGAGCTTTCAAGCCCCACGTTTTCCCAGTTCGAAGTCTCCTGCTTCCTCTGTCCAGCACAGAGACATTGATAGGCAATCACAAGAGTTGCTTAATGGGATGGTAACTCCTCCTAGTGTGATGTTTGAGGAATCTGTTGGGAACAACAAAAATGAAAATCAGGATGATAGCATCTGGTTGAAGGAAAAATGGGCAATTGGGGAGATACCGCTGGATGAACCACCTCAGAGGCACGTTAGCCATGTGTTTTAAGAACTCAAAGGTGTTTTTTTTTGTGTTGTTGAGGCATGAGACCATGAACTACTCAGCTATGATCTGACTTCCTTGTAGTTGGGTTGCCACCATATGTCGTACTTTTCTTGCCCGAGCTTCATGACGATACAAAGGAATTATACAACAAACAAGAGAATTTGCAAGAGTGAACAGTGAAGGAAGGCTGATGAACCAATGCATTGTTTGGTACCACTTATTTATTCCTGGAACATGAATGTGGGCAACTAGAGCTAACAACCAGCTAACTGCAGTCTTGCCTGTCGAGTTAGTATGTTTTTCTCTCTTTTTTTTAATCTGTAATTGTTTATTCCAATAGTATGTTTTTCTTTTTTTTTAATCTGTAATTGTTTATTCCAAAACTTGTTAGGAAGTTATTGGAATAAGAAACAGTATTGCAATATTGAGTGATTGGCCGGGTAACA

CDS Sequence

>LOC_Os06g49080.1
ATGAGTCGGCGGCAGGAGATTTGCCGCAACTTCCAGCGCGGAAGTTGCAAGTACGGAGCGCAGTGCAGGTATTTGCATGCCTCCCCTCACCAGCAACAGCAGCAGCAGCAGGCGAAGCCCAACCCGTTTGGATTTGGCACCGGAAGCAGGCAGCAGCAGCAGCCGTCATTTGGCTCACAGTTCCAGCAGCAGCAACAGCAGCAGCAGAAGCCCAATCCTTTTGGTTTTGGGGTACAAGGTGCCAATGCCCAGTCACGTAATGCTCCTGGTCCTGCGAAGCCTTTTCAAAATAAGTGGGTAAGGGACCCCTCGGCCCCGACGAAGCAAACAGAGGCGGTGCAGCCACCGCAGGCGCAGGCTGCCCACACCTCCTGTGAAGACCCTCAGTCATGCAGACAACAAATTTCTGAGGATTTTAAGAACGAGGCTCCTATTTGGAAGCTTACTTGTTATGCTCATCTCAGAAATGGCCCTTGCAATATTAAGGGAGACATTAGCTTCGAGGAACTAAGAGCTAAAGCCTATGAGGAAGGAAAGCAAGGGCATTCTCTGCAATCAATAGTTGAGGGAGAAAGAAATCTACAAAACGCAAAGTTAATGGAGTTTACTAATCTGCTCAATAGTGCACGTCCATCACAAACTCCAAGCTTTCCTACTATGAGTTCCTTCCCTGAAGTGAAAAACAACTCATCATTCGGGGCTTCTCAAACCAATGGACCACCTGTGTTCAGTAGTTTCAGTCAAATTGGAGCAGCAACTAATATTGGACCTGGGCCAGGAACCACCGCACCAGGAATGCCAGCCAGTAGTCCTTTTGGTCATCCAAGCTCTGCACCACTTGCTGCCCCTACTTTTGGCAGTTCTCAAATGAAGTTTGGAGTTTCGAGCGTATTTGGAAACCAGGGTTCAGGTCAACCATTTGGGAGCTTTCAAGCCCCACGTTTTCCCAGTTCGAAGTCTCCTGCTTCCTCTGTCCAGCACAGAGACATTGATAGGCAATCACAAGAGTTGCTTAATGGGATGGTAACTCCTCCTAGTGTGATGTTTGAGGAATCTGTTGGGAACAACAAAAATGAAAATCAGGATGATAGCATCTGGTTGAAGGAAAAATGGGCAATTGGGGAGATACCGCTGGATGAACCACCTCAGAGGCACGTTAGCCATGTGTTTTAA

Protein Sequence

>LOC_Os06g49080.1
MSRRQEICRNFQRGSCKYGAQCRYLHASPHQQQQQQQAKPNPFGFGTGSRQQQQPSFGSQFQQQQQQQQKPNPFGFGVQGANAQSRNAPGPAKPFQNKWVRDPSAPTKQTEAVQPPQAQAAHTSCEDPQSCRQQISEDFKNEAPIWKLTCYAHLRNGPCNIKGDISFEELRAKAYEEGKQGHSLQSIVEGERNLQNAKLMEFTNLLNSARPSQTPSFPTMSSFPEVKNNSSFGASQTNGPPVFSSFSQIGAATNIGPGPGTTAPGMPASSPFGHPSSAPLAAPTFGSSQMKFGVSSVFGNQGSGQPFGSFQAPRFPSSKSPASSVQHRDIDRQSQELLNGMVTPPSVMFEESVGNNKNENQDDSIWLKEKWAIGEIPLDEPPQRHVSHVF*