Information report for OsHKT2;1;OsHKT1
Gene Details
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Functional Descriptions
- No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na(+) concentrations, in contrast to OsHKT2;1.
- The OsHKT2;1 (previously named OsHKT1) transporter from rice functions as a relatively Na+-selective transporter in heterologous expression systems, but the in vivo function of OsHKT2;1 remains unknown.
- These analyses demonstrate that Na+ can enhance growth of rice under K+ starvation conditions, and that OsHKT2;1 is the central transporter for nutritional Na+ uptake into K+-starved rice roots.
- Rice OsHKT2;1 transporter mediates large Na+ influx component into K+ -starved roots for growth.
- In addition, the presence of external K(+) and Ca(2+) down-regulated OsHKT2;1-mediated Na(+) influx in two plant systems, Bright-Yellow 2 cells and intact rice roots, and also in Xenopus oocytes.
- In roots, the expressions were much lower than in shoots, except for OsHKT4 and OsHKT1 in K+-starved plants.
- We propose that OsHKT transporters are involved in Na+ movements in rice, and that OsHKT1 specifically mediates Na+ uptake in rice roots when the plants are K+ deficient.
- Yeast expressions of OsHKT1 and OsHKT4 proved that they are Na+ transporters of high and low affinity, respectively, which are sensitive to K+ and Ba2+.
- Parallel experiments of K+ and Na+ uptake in yeast expressing the wheat or rice HKT1 transporters proved that they were very different; TaHKT1 transported K+ and Na+, and OsHKT1 only Na+.
- Upon NaCl stress, the OsHKT1 transcript was significantly down-regulated in salt-tolerant cv.
- Pokkali regulates the expression of OsHKT1, OsHKT2, and OsVHA differently from how the salt-sensitive cv.
- Expressions of OsHKT1, OsHKT2, and OsVHA are differentially regulated under NaCl stress in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars.
- OsHKT2;1 was mainly expressed in the cortex and endodermis of roots.
- (22)Na+ tracer influx experiments revealed that Na+ influx into OsHKT2;1-null roots was dramatically reduced compared with wild-type plants.
- Therefore, here we analyze two highly homologous rice (Oryza sativa) HKT transporters in plant cells, OsHKT2;1 and OsHKT2;2, that show differential K(+) permeabilities in heterologous systems.
- Differential sodium and potassium transport selectivities of the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells.
- We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins.
- The OsHKT1, OsHKT2, and OsVHA transporter genes might play important roles in maintaining cytosolic Na(+) homeostasis in rice (Oryza sativa L.
- Interestingly, three independent OsHKT2;1-null alleles exhibited significantly reduced growth compared with wild-type plants under low Na+ and K+ starvation conditions.
- Of these, the sodium transporter gene OsHKT2;1 emerged as a key factor that impacts on KUE based on (i) the correlation between shoot Na+ and KUE, and (ii) higher levels of HKT2;1 expression in high KUE lines.
- Expression levels of the Na+/K+ transporter OsHKT2;1 and vacuolar Na+/H+ exchanger OsNHX1, Na enrichment, maintaining the photosynthetic abilities and growth performances of indica rice seedlings under salt stress.
- The aim of this investigation was to determine the OsNHX1 (a vacuolar Na+/H+ exchanger) and OsHKT2;1 (Na+/K+ transporter) regulation by salt stress (200<U+00A0>mM NaCl) in two rice cultivars, i.
- Pokkali (15<U+00A0>min after salt stress) due to the higher expression of OsHKT2;1 gene (by 2.
- Correspondingly, null mutants of OsHKT2;1 displayed an increased tolerance to salt stress.
- Under salt stress treatment, the expression levels of OsbZIP05, OsHKT1;1 and OsDREB1B were significantly lower yet the level of OsHKT2;1 was significantly higher in oswrky28 mutants than those in wide type plants.
Functional Keywords
- root , transporter , salt , potassium , homeostasis , growth , shoot , salt-tolerance , seedlings , salt-stress , stress , tolerance
Literature and News
- Rice OsHKT2;1 transporter mediates large Na+ influx component into K+-starved roots for growth . DOI: 10.1038/sj.emboj.7601732 ; PMID: 17541409
- Expressions of OsHKT1, OsHKT2, and OsVHA are differentially regulated under NaCl stress in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars . DOI: 10.1093/jxb/erl199 ; PMID: 17088362
- Sodium transport and HKT transporters: the rice model . DOI: 10.1046/j.1365-313x.2003.01764.x ; PMID: 12795699
- HKT2;2/1, a K⁺-permeable transporter identified in a salt-tolerant rice cultivar through surveys of natural genetic polymorphism . DOI: 10.1111/j.1365-313X.2012.05031.x ; PMID: 22530609
- Differential sodium and potassium transport selectivities of the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells . DOI: 10.1104/pp.109.145722 ; PMID: 19889878
- OsHKT2;2/1-mediated Na(+) influx over K(+) uptake in roots potentially increases toxic Na(+) accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress . DOI: 10.1007/s10265-015-0764-1 ; PMID: 26578190
- A role for the OsHKT 2;1 sodium transporter in potassium use efficiency in rice . DOI: 10.1093/jxb/erz113 ; PMID: 30854552
- Expression levels of the Na+/K+ transporter OsHKT2;1 and vacuolar Na+/H+ exchanger OsNHX1, Na enrichment, maintaining the photosynthetic abilities and growth performances of indica rice seedlings under salt stress . DOI: 10.1007/s12298-020-00769-3 ; PMID: 32205927
- Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis . DOI: 10.15252/embj.2020105086 ; PMID: 33347628
- CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice . DOI: 10.1016/j.jbiotec.2021.02.008 ; PMID: 33610657
- OsWRKY28 positively regulates salinity tolerance by directly activating OsDREB1B expression in rice . DOI: 10.1007/s00299-022-02950-2 ; PMID: 36350394
- Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances . DOI: 10.3389/fpls.2023.1095929 ; PMID: 37008489
Gene Resources
- UniProt: Q0D9S3
- EMBL: AB061311, AJ491852, AP003726
- AlphaFoldDB: Q0D9S3
- EnsemblPlants: Os06t0701700-01
- Gramene: Os06t0701700-01
- KEGG: dosa:Os06g0701700
Sequences
cDNA Sequence
- >LOC_Os06g48810.1
AAAGGACACTCACTTTGCTTTCTCATACTCGTTGGCTCGTTGCTCCTTTGCATCAAATGACGAGCATTTACCATGATTTCATTCATAACAAGCTGCAGAGCTTCGGAAGGATCGGTAGATATTTTGTTAATTTTGTTGTTCTAGCCCATAGATTCATTGCCTTGCATATTCACCCATTCTGGATTCAGTTGTCCTACTTCCTTCTCATTAGCATCCTAGGTTCAGTTCTATTGATGTTCCTGAAGCCAAGCAACCCAGAATTCAGACCAGGTTACATTGACATGCTGTTCTTGTCAACCTCTGCTCTGACACTTTCCAGCCTCATCACCATCGAAATGGAGGTTCTCTCAAGCTCACAAATTGTGGTTATTACACTGCTGATGCTTCTCGGGGGTGAAGTCTTTGTTTCTTTCCTAGGCCTCATGCTTAGACTGAACCATAAGCACAACCCAGAGTTTTCAGGGGACAAGGTCAGTTCAGTTCCTATCGAGCTTGATACAATCAACTCGGCAAGCACTGTGATAAGTTGTGAAGAGCTGCAGCTGGAAGCAGCAATTCCTGAAGTTCCATCTTCCACCATTAAGGATTTGAAGAGGAGCAAGCGCCTGAGGTGGTTCTTAGGATTTGTAGTCTTCAGCTATTTTGTTGTGATCCATGTCGCTGGCTTTCTGCTGGTTCTCTGGTACATAAGTCGTGTCTCATCTGCAAAAGCTCCACTGAAGAAGAAAGGGATCAACATTGCACTCTTCTCATTCTCGGTCACAGTCTCCTCGTTTGCGAATGTGGGGCTCGTGCCGACGAATGAGAACATGGCAATCTTCTCCAAGAACCCGGGCCTCCTCCTCCTGTTCATCGGCCAGATTCTTGCAGGCAATACACTTTACCCTCTCTTCCTAAGGCTATTGATATGGTTCCTGGGGAAGGTGACCAAGTTGAGAGAACTGAAGCTCATGATCAAGAACCCTGAGGAGCTACAGTATGATTATTTGCTTCCTAAGTTGCCAACGGCGTTTCTGGCATCAACTGTCATTGGCCTTATGGCTTCCTTGGTCACATTGTTCGGTGCTGTCGACTGGAATTCTTCAGTCTTTGATGGACTCAGCTCTTACCAGAAGATTATCAATGCATTGTTCATGGCAGTGAACGCAAGGCACTCGGGGGAGAACTCCATCGACTGCTCACTCATCGCCCCTGCTGTTCTAGTACTGTTCATCATCTTAATGTACTTACCACCATCGACAACATTTGCACTGTCCAATGGAGATGAAAAAACTGCAAATAAGAAAGCGAAAAGAAAATTAGGTTTAGTGGTGCAGAACTTGGCATTTTCACAGCTTGCCTGCATCTCAGTCTTTGTGATAGTTGCATTCATCACTGAGAGGAGTCGTCTCAGAAATGATCCACTCAACTTCTCTGCTCTGAACATGATATTTGAAATCATCAGTGCATATGGGAATGTAGGGCTATCCACTGGTTACAGCTGTTCGAGGTTGCAGAAACTGCACCCGGGGAGCATCTGCCAGGACAAGCCGTACAGCTTGTCCGGATGGTGGAGTGATGAAGGAAAGTTGCTGCTAGTCTTTGTGATGCTCTATGGAAGGCTCAAGGCCTTCACAAAAGGTACAGGTGAATATTGGAGGCTATGGTAAAAGCTGAAGCCTGAACAAAAGCACTTGCAAAGGCAAGTGCATCAAGTGCAGTATATGTAACTATTTAGAGTTCTGCCTGAAAGTTCAGGTTTCTAATTGGGCCATAGTGTATAAAAATACACCCAATATTATTCCTCTTAATACAGTGGTGCGCAATCCTTTAGCGTATTCCCGAAAAAAAGAACACCCAATATGTTGCAATTGCAATATAGAACTGTTGTTCTCCAACCAA
CDS Sequence
- >LOC_Os06g48810.1
ATGACGAGCATTTACCATGATTTCATTCATAACAAGCTGCAGAGCTTCGGAAGGATCGGTAGATATTTTGTTAATTTTGTTGTTCTAGCCCATAGATTCATTGCCTTGCATATTCACCCATTCTGGATTCAGTTGTCCTACTTCCTTCTCATTAGCATCCTAGGTTCAGTTCTATTGATGTTCCTGAAGCCAAGCAACCCAGAATTCAGACCAGGTTACATTGACATGCTGTTCTTGTCAACCTCTGCTCTGACACTTTCCAGCCTCATCACCATCGAAATGGAGGTTCTCTCAAGCTCACAAATTGTGGTTATTACACTGCTGATGCTTCTCGGGGGTGAAGTCTTTGTTTCTTTCCTAGGCCTCATGCTTAGACTGAACCATAAGCACAACCCAGAGTTTTCAGGGGACAAGGTCAGTTCAGTTCCTATCGAGCTTGATACAATCAACTCGGCAAGCACTGTGATAAGTTGTGAAGAGCTGCAGCTGGAAGCAGCAATTCCTGAAGTTCCATCTTCCACCATTAAGGATTTGAAGAGGAGCAAGCGCCTGAGGTGGTTCTTAGGATTTGTAGTCTTCAGCTATTTTGTTGTGATCCATGTCGCTGGCTTTCTGCTGGTTCTCTGGTACATAAGTCGTGTCTCATCTGCAAAAGCTCCACTGAAGAAGAAAGGGATCAACATTGCACTCTTCTCATTCTCGGTCACAGTCTCCTCGTTTGCGAATGTGGGGCTCGTGCCGACGAATGAGAACATGGCAATCTTCTCCAAGAACCCGGGCCTCCTCCTCCTGTTCATCGGCCAGATTCTTGCAGGCAATACACTTTACCCTCTCTTCCTAAGGCTATTGATATGGTTCCTGGGGAAGGTGACCAAGTTGAGAGAACTGAAGCTCATGATCAAGAACCCTGAGGAGCTACAGTATGATTATTTGCTTCCTAAGTTGCCAACGGCGTTTCTGGCATCAACTGTCATTGGCCTTATGGCTTCCTTGGTCACATTGTTCGGTGCTGTCGACTGGAATTCTTCAGTCTTTGATGGACTCAGCTCTTACCAGAAGATTATCAATGCATTGTTCATGGCAGTGAACGCAAGGCACTCGGGGGAGAACTCCATCGACTGCTCACTCATCGCCCCTGCTGTTCTAGTACTGTTCATCATCTTAATGTACTTACCACCATCGACAACATTTGCACTGTCCAATGGAGATGAAAAAACTGCAAATAAGAAAGCGAAAAGAAAATTAGGTTTAGTGGTGCAGAACTTGGCATTTTCACAGCTTGCCTGCATCTCAGTCTTTGTGATAGTTGCATTCATCACTGAGAGGAGTCGTCTCAGAAATGATCCACTCAACTTCTCTGCTCTGAACATGATATTTGAAATCATCAGTGCATATGGGAATGTAGGGCTATCCACTGGTTACAGCTGTTCGAGGTTGCAGAAACTGCACCCGGGGAGCATCTGCCAGGACAAGCCGTACAGCTTGTCCGGATGGTGGAGTGATGAAGGAAAGTTGCTGCTAGTCTTTGTGATGCTCTATGGAAGGCTCAAGGCCTTCACAAAAGGTACAGGTGAATATTGGAGGCTATGGTAA
Protein Sequence
- >LOC_Os06g48810.1
MTSIYHDFIHNKLQSFGRIGRYFVNFVVLAHRFIALHIHPFWIQLSYFLLISILGSVLLMFLKPSNPEFRPGYIDMLFLSTSALTLSSLITIEMEVLSSSQIVVITLLMLLGGEVFVSFLGLMLRLNHKHNPEFSGDKVSSVPIELDTINSASTVISCEELQLEAAIPEVPSSTIKDLKRSKRLRWFLGFVVFSYFVVIHVAGFLLVLWYISRVSSAKAPLKKKGINIALFSFSVTVSSFANVGLVPTNENMAIFSKNPGLLLLFIGQILAGNTLYPLFLRLLIWFLGKVTKLRELKLMIKNPEELQYDYLLPKLPTAFLASTVIGLMASLVTLFGAVDWNSSVFDGLSSYQKIINALFMAVNARHSGENSIDCSLIAPAVLVLFIILMYLPPSTTFALSNGDEKTANKKAKRKLGLVVQNLAFSQLACISVFVIVAFITERSRLRNDPLNFSALNMIFEIISAYGNVGLSTGYSCSRLQKLHPGSICQDKPYSLSGWWSDEGKLLLVFVMLYGRLKAFTKGTGEYWRLW*