Gene Details:
- MSU gene ID: LOC_Os06g48530
- RAPdb gene ID: Os06g0698859
- Gene Symbol: Du13 TL1
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Map-based cloning showed that Du13 encodes a C(2) H(2) zinc-finger protein.
- Du13 encodes a C(2) H(2) zinc-finger protein that regulates Wx(b) pre-mRNA splicing and microRNA biogenesis in rice endosperm.
- Moreover, Du13 also regulates alternative splicing of other protein-coding transcripts and affects the biogenesis of a subset of microRNAs.
- Du13 coordinates with the nuclear cap-binding complex to regulate the splicing of Wx(b) transcripts in rice endosperm.
- Loss of TL1 function reduced apparent amylose content (AAC), total starch, gel consistency, and gelatinisation temperature, whereas increased viscosity, total lipid, and ratio of amylopectin A chains with degree of polymerization (DP) 6-12 to B(1) chains with DP 13-24, resulting in an enhanced grain ECQ.
- Here, we report the cloning and characterization of a novel C(2)H(2) zinc finger protein TL1 (Translucent endosperm 1) that positively regulates amylose synthesis in rice grains.
Function-related keywords:
- map-based-cloning , R-protein , endosperm , grain , zinc , amylose-content
Literature:
- Du13 encodes a C2 H2 zinc-finger protein that regulates Wxb pre-mRNA splicing and microRNA biogenesis in rice endosperm . DOI: 10.1111/pbi.13821 ; PMID: 35560858
- Mutation of TL1, encoding a novel C2H2 zinc finger protein, improves grains eating and cooking quality in rice . DOI: 10.1007/s00122-022-04198-6 ; PMID: 35994056
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os06g48530.1
GTGCGCCGCAGCCCGCAGCCTAGCCTACCCTCTCTCTCTCCCCCCTCCTCAAGCTGTGCGCGATTCGCTTCCTCACTCCCAAACCCTAACCCCACCGCGACGCTCCCCCCCATGGCTGACGTCATCGACCCCGCCTCCACCGAGGCCCCCCGCGCGCGCCGCCCGCCGCCGCCTCCGCCCGACAGCCCGGAGGGCCGCTCGCCGCCGCTCCCGCCCCCGCCCCCCGGTGGCCCGCCGCAGCCGGCGGCCACCCGCAAGCGGAGCCGCTCGCCACCGCCGCCTCCCCCGCCGCCCTCCCTCCCGCCGCCCCCGCCGCTCGGCTCGTCGCGCCCCGAGCGCTACCGCGACAACCACCACCGGGGAGGAGGCGGTGGCCGGGGTGGGGGTAGTTCCAGCCCCCCGCCGTATCGGAGTGGCCGCCGCCACTCCCCGTCGAGGAGATCCCCTTCGCCGCCGTTCAAGAGGTCGCGGCGGGACGACGGGTACGACCGCCGTGGCGGCCGTGGGAGCCCGCCGCCGCGGTACGGGTACGGCGACAGGAGGTATGGATATGACCACGAGCGTGGTGGAGGCAGAGGTGGGTATGATGATGACCGATACCATGGCAGGTATCAAAATCGCGCAGCAGATTGGGCCGATTCAGGGTTTGGGGCATCCAATGATGGTCCTGGAATTACCCAAAGGGAAGGACTGATGACTTACAAACAGTTCATCCAAGTTCTTGAGGATGATATTTCACCTGCTGAAGCTGAGAAACGGTATCAAGAATACAGGACAGAGTACATCACTACTCAAAAACGTGCTTATTTTGACCTTAACAAGAATGATGATCGGTTGAAAGACAAGTACCATCCGACCAACTTGTCATCTGTTATTGACAGGAGGAATGATAGTTGTAAGGCAACAGCAAAGGATTTCTTTCATGATTTGCAAAATGGAACTCTGGACCTTGGCCCTGGAATAACTGCGGCTGCAGCAAGTGGCAGTGATGGAAATTCTGATGATGATGGAGACAGTGACAAGAGAAGAAAGCATGGCAGGGGTTCCTCAAAAGAAACAGACCCTCTTTCTGGTGCTCCCGTGGCTCATCCAGTTAGCTCTGAATCTCGACGGGTTCAAGTTGACATTGAACAAGCTCTAGCCCTTGTGCGTAAGCTTGACACTGAGAAGGGTATTGTGGGGAATATCCTATCAAGTGGCGATCATGACAAATCAGATGTAGACAAGTCTCATATTGGATCTATGGGGCCTATAATTATAATCCGAGGCTTAACCACTGTCAAAGGCCTTGAAGGTGTTGAGCTCCTAGATACTCTTCTTACCTATTTATGGCGTATTCATGGTGTTGATTACTATGGCATGTCTGAGACAAATGAAGCAAAAGGCAGTCGCCATGTCAGAGCAGACAATAAGACGTCTAATACAACCAATATTAATGCCGCTGACTGGGAAAAGAAGGTGGATACTTTCTGGCAAGAAAGGCTGAGAGGTCAGGACCCCATGGTAATATTAGCAGCCAAGGACAAAATCGATGCAGCAGCTGTGGAAGTTCTGGAACCTTATGTCAGGAAGATAAGGGATGAAAAATATGGTTGGAAATATGGCTGTGGAGCTAAGGGTTGTACGAAACTTTTCCATGCTCCTGAGTTCGTTCACAAGCATTTGAGGCTGAAGCATCCAGAGCTTGTGTTAGAGTTGACTTCCAAAGTCCGAGAGGATCTCTATTTCCAAAATTACATGAATGATCCTAATGCACCTGGTGGAACTCCAGTTATGCAACAGTCTGCACCAGACAAATCAAGACAGAGACCTGGTATGGATAATCGTCTGAGATATGACCGTGCCAATCGTAGAGAATATGATAGGGCAGAGAGAGATGGAAGCAGATATGGTAGAGGTGATCGTTCTCCAAGTCTTGATGGCGCTGATGATCAGATGTTTGATGCTTTCCGTGGGCGAGGTCCAAATGCTCCTTTTGTTCCTGAACTTCCCGCTCCGCCAATTTTGATGCCTATTCCTGGTGCTGGTCCTTTGGGTCCATTTGTTCCTGCACCTCCAGAAATAGCCATGCATATGCTGAGAGAGCAAGGGCCGCCACCTCCATTTGAACCAAACGGACCTCCTCATGCCAACCCAGGAGTGCTTGGACCAATGATGGGTGGTCCTGCGCCAATTATAACCATGCCTCCATCTTTTCGTCAAGATCCTCGCCGTTTGCGAAGTTACAATGACCTTGATGCTCCGGACGAGGAAGTTACCGTTCTTGACTACAGAAGTTTGTAGAGCTTGCCCTGGTGTAATTGTAATTTGCCAATCACAACTCTAGCATCTCCGGTCTAGTCTAGGTTGGTGATGTATTCTTTTTCAGACATAGGGGATGTCATGAACAATAGAGCATTTTTTGAGGTGTAATGCGTCAGAAACTACTGTTGTAATTTCAAATGGCAACATCTGTTATTGAACTGTGCACCACGTGCACTTGTAGTCCCAAGAAGTGTTGAACGCAGTTTGATAAAATGTAATTTTTGAGACTTTATATGACAACGTTTTAGCTGACAACATTTTACTTCTCCATTGGAAGTAATTAATTTATTCCTTCAGTTGGAACTTGGGAGAGG
CDS Sequence
- >LOC_Os06g48530.1
ATGGCTGACGTCATCGACCCCGCCTCCACCGAGGCCCCCCGCGCGCGCCGCCCGCCGCCGCCTCCGCCCGACAGCCCGGAGGGCCGCTCGCCGCCGCTCCCGCCCCCGCCCCCCGGTGGCCCGCCGCAGCCGGCGGCCACCCGCAAGCGGAGCCGCTCGCCACCGCCGCCTCCCCCGCCGCCCTCCCTCCCGCCGCCCCCGCCGCTCGGCTCGTCGCGCCCCGAGCGCTACCGCGACAACCACCACCGGGGAGGAGGCGGTGGCCGGGGTGGGGGTAGTTCCAGCCCCCCGCCGTATCGGAGTGGCCGCCGCCACTCCCCGTCGAGGAGATCCCCTTCGCCGCCGTTCAAGAGGTCGCGGCGGGACGACGGGTACGACCGCCGTGGCGGCCGTGGGAGCCCGCCGCCGCGGTACGGGTACGGCGACAGGAGGTATGGATATGACCACGAGCGTGGTGGAGGCAGAGGTGGGTATGATGATGACCGATACCATGGCAGGTATCAAAATCGCGCAGCAGATTGGGCCGATTCAGGGTTTGGGGCATCCAATGATGGTCCTGGAATTACCCAAAGGGAAGGACTGATGACTTACAAACAGTTCATCCAAGTTCTTGAGGATGATATTTCACCTGCTGAAGCTGAGAAACGGTATCAAGAATACAGGACAGAGTACATCACTACTCAAAAACGTGCTTATTTTGACCTTAACAAGAATGATGATCGGTTGAAAGACAAGTACCATCCGACCAACTTGTCATCTGTTATTGACAGGAGGAATGATAGTTGTAAGGCAACAGCAAAGGATTTCTTTCATGATTTGCAAAATGGAACTCTGGACCTTGGCCCTGGAATAACTGCGGCTGCAGCAAGTGGCAGTGATGGAAATTCTGATGATGATGGAGACAGTGACAAGAGAAGAAAGCATGGCAGGGGTTCCTCAAAAGAAACAGACCCTCTTTCTGGTGCTCCCGTGGCTCATCCAGTTAGCTCTGAATCTCGACGGGTTCAAGTTGACATTGAACAAGCTCTAGCCCTTGTGCGTAAGCTTGACACTGAGAAGGGTATTGTGGGGAATATCCTATCAAGTGGCGATCATGACAAATCAGATGTAGACAAGTCTCATATTGGATCTATGGGGCCTATAATTATAATCCGAGGCTTAACCACTGTCAAAGGCCTTGAAGGTGTTGAGCTCCTAGATACTCTTCTTACCTATTTATGGCGTATTCATGGTGTTGATTACTATGGCATGTCTGAGACAAATGAAGCAAAAGGCAGTCGCCATGTCAGAGCAGACAATAAGACGTCTAATACAACCAATATTAATGCCGCTGACTGGGAAAAGAAGGTGGATACTTTCTGGCAAGAAAGGCTGAGAGGTCAGGACCCCATGGTAATATTAGCAGCCAAGGACAAAATCGATGCAGCAGCTGTGGAAGTTCTGGAACCTTATGTCAGGAAGATAAGGGATGAAAAATATGGTTGGAAATATGGCTGTGGAGCTAAGGGTTGTACGAAACTTTTCCATGCTCCTGAGTTCGTTCACAAGCATTTGAGGCTGAAGCATCCAGAGCTTGTGTTAGAGTTGACTTCCAAAGTCCGAGAGGATCTCTATTTCCAAAATTACATGAATGATCCTAATGCACCTGGTGGAACTCCAGTTATGCAACAGTCTGCACCAGACAAATCAAGACAGAGACCTGGTATGGATAATCGTCTGAGATATGACCGTGCCAATCGTAGAGAATATGATAGGGCAGAGAGAGATGGAAGCAGATATGGTAGAGGTGATCGTTCTCCAAGTCTTGATGGCGCTGATGATCAGATGTTTGATGCTTTCCGTGGGCGAGGTCCAAATGCTCCTTTTGTTCCTGAACTTCCCGCTCCGCCAATTTTGATGCCTATTCCTGGTGCTGGTCCTTTGGGTCCATTTGTTCCTGCACCTCCAGAAATAGCCATGCATATGCTGAGAGAGCAAGGGCCGCCACCTCCATTTGAACCAAACGGACCTCCTCATGCCAACCCAGGAGTGCTTGGACCAATGATGGGTGGTCCTGCGCCAATTATAACCATGCCTCCATCTTTTCGTCAAGATCCTCGCCGTTTGCGAAGTTACAATGACCTTGATGCTCCGGACGAGGAAGTTACCGTTCTTGACTACAGAAGTTTGTAG
Protein Sequence
- >LOC_Os06g48530.1
MADVIDPASTEAPRARRPPPPPPDSPEGRSPPLPPPPPGGPPQPAATRKRSRSPPPPPPPPSLPPPPPLGSSRPERYRDNHHRGGGGGRGGGSSSPPPYRSGRRHSPSRRSPSPPFKRSRRDDGYDRRGGRGSPPPRYGYGDRRYGYDHERGGGRGGYDDDRYHGRYQNRAADWADSGFGASNDGPGITQREGLMTYKQFIQVLEDDISPAEAEKRYQEYRTEYITTQKRAYFDLNKNDDRLKDKYHPTNLSSVIDRRNDSCKATAKDFFHDLQNGTLDLGPGITAAAASGSDGNSDDDGDSDKRRKHGRGSSKETDPLSGAPVAHPVSSESRRVQVDIEQALALVRKLDTEKGIVGNILSSGDHDKSDVDKSHIGSMGPIIIIRGLTTVKGLEGVELLDTLLTYLWRIHGVDYYGMSETNEAKGSRHVRADNKTSNTTNINAADWEKKVDTFWQERLRGQDPMVILAAKDKIDAAAVEVLEPYVRKIRDEKYGWKYGCGAKGCTKLFHAPEFVHKHLRLKHPELVLELTSKVREDLYFQNYMNDPNAPGGTPVMQQSAPDKSRQRPGMDNRLRYDRANRREYDRAERDGSRYGRGDRSPSLDGADDQMFDAFRGRGPNAPFVPELPAPPILMPIPGAGPLGPFVPAPPEIAMHMLREQGPPPPFEPNGPPHANPGVLGPMMGGPAPIITMPPSFRQDPRRLRSYNDLDAPDEEVTVLDYRSL*