Gene Details:

Functional Descriptions:

  • Expression analyses of the chloroplast-deficient rice mutant, virescent-1, showed a discrepancy between OsRpoTp protein accumulation and the level of transcripts of NEP-transcribed genes.
  • Accumulation of transcripts of nuclear-encoded photosynthetic genes, such as cab and rbcS, was strongly suppressed in the mutant at later stages of chloroplast differentiation, whereas transcripts of genes for the plastid transcription apparatus, such as OsRpoTp and OsSIG2A, accumulated to abnormally high levels at these stages.
  • We demonstrated by reverse transcription-PCR experiments and immunoblot analysis that OsRpoTp expression occurred at an early stage of leaf development, prior to the transcript accumulation of the genes that were transcribed by the nuclear-encoded plastid RNA polymerase (NEP).

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g44230.1
    ACCTTCCTCTCCCCAAAGTCCAAGGTTCTACCGGGGTTTCAGATTTCTCTCTCTTCCTCTACCACCTCCGCCGCTGCTCGTCGTCACCGACCTCCAAATCCGAACCAAAATTCTCTCGCCATTGCGGGGATTGGAGCTCCAAGGGATAAGCTCTGCCTGCCCTAGGCGCCGCATGCCTCTCCTCCTCTTCCCCATCTCCCCTCCCTGCGTGCCCCCGCCGCGGCCCCGCCTACGCCGCCTCTCCCCGCCGCCGCCCATGGCGGCCGTCGCGCCGCCGTCGCTGAGCACGCCCGTGACCATACTCCCCTCCGTGTCTGTCGCCTTGCCCCCGCTTCCACCCCCTGCTACTGATGACTTCCACTGGCTCGACCTCTTCGCGTTCCTCAACTCCCCCGCGGATTCCTACCAGATTCCTGTGGAGGAGCAGGAGGTGGAGGTGGAGGTGGAGGTGGAGGTGGGGGTGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGCGGGCGAGGAAGGCGGAGCACCGGCGGCTGCGGCAGCGGCAGGTGAAGGCGGAGACGGAGGCGTGGGCGCGGGCCGCCGACGAGTACCGGGAGCTCGAGCGGGAGATGCTCGACCGGAGGCTCGCGCCCGCGCTGCCCTACGTCAAGTCGCTCTTCCTCGGCTGGTTCGAGCCGCTGCGCGACGCCATCGCCCGGGACCAGGAGGTGCAGCGGCGGAAGCGGGTCAAGCACGTCTACGCCAAGTACCTGCTCCTGCTGCCCGCGGACAAGGTGGCCGTCATCGTCATGCACAAGATGATGGGCCTCCTCATGTCGAGCAAGGATGGCGTCGCCAGCGTCCGTGTTGTCCAGGCCGCGCACTGCATCGGCGAGGCTGTTGAACGTGAGTTCAAAGTCCAGACATTCTTTCAAAAGACAAGGAAGAAAAGTGCAGGTGAAAATGATTTGGCATTAGAGAAGGAGCAGGCCAAATGCCGAAAACGTGTTAAGAGTTTGGTTAGGAGGAGAAAGTTGACTGAAGCACAGAAGATTGTGCAACAGGAGATTGAATTGGAAGAATGGGGCACAGAATCCCAAGTGAAGTTAGGGACCCGCTTGATTGAGTTGTTACTGGATTCAGCTTTTGTACAGTCACCAGCTGACCAAACACCAGAGAGTTCTCCAGATATTCGACCAGCATTTAAACACGTACTTCGACAACCTATAGTAGAAAATGGGAGACTGAAGAAAAAGCACTGGGTGATAGAGTGTGACCCTCTTGTGCATGAGGGGTTTGAAAGCACTGCTAGGCACGTGGAGATACCCTATCTTCCTATGCTTGTGACTCCTAAAAAATGGAAGGGCTATGATACAGGTGGGTACCTTTTTCTTCCCTCATATATTATGCGGACACATGGCGTGAAAGATCAAAAGGAAGCCATTAAGAGTGTTCCGAGAAAACAGCTACGGAAAGTATTTGAGGCATTAGATACCCTTGGGAGTACTAAATGGAGGGTGAATAGAAGAGTACATAATGCTGTTGAAACTATTTGGAGTCGAGGTGGGGGCATTGCAGGACTGGTTGATAAGGAAAATATTCCTCTACCTGAACGACCAGAAACGGAGGATCCAGATGAAATACAGAAATGGAAGTGGAGCTTAAAGAAGGCAAAGAAAGCTAATCGTGAACTGCATGCTGAGCGATGCGATACAGAGCTTAAACTATCTGTTGCTCGGAAAATGAGAGAGGAGGATGGATTTTATTATCCCCATAATCTTGATTTTCGTGGCCGTGCATACCCTATGCATGCACATCTAAGCCACCTAGGTTCAGATCTATGCAGAGGTGTTCTAGAGTATGCTGAAGGGCGGCCACTAGGAAAGTCTGGCTTACGCTGGTTAAAGATTCATTTGGCTAACAAATATGGTGGAGGAATTGAAAAGCTTTCTCATGAGGACAAGGTAGCATTTGTGGAGAACCAGTTGCCTGATATATTTGATTCAGCAACTAATCCTGTTGACGGGAATTGCTGGTGGATGAATGCTGAGGATCCTTTTCAATGTTTAGCCGCATGCATGGATCTTTCTGATGCCTTAAAAAGTTCATCACCTCAGTGTGCTGTTTCTCACCTACCTATTCATCAGGATGGTTCATGCAATGGTTTACAACACTATGCTGCTCTTGGAAGAGACTATATGGGTGCAGCTGCTGTCAATTTAGTTCCTGGAGACAAACCTGCAGATATCTACTCAGAGATTGCTGCTAGGGTGCTGGATGTTGTTCGGGAAGACTCGATGGAGGATCCTGCAACTAATCCAACTGCCTCATTAGCAAGGGTTTTAGTCGATCAGGTGGATAGGAAGCTTGTTAAGCAGACAGTGATGACATCAGTTTACGGTGTCACATATATTGGTGCTCGTCAACAGATAACAAAAAGACTACAAGAGAAAGGGCTCATTACAGATGACAAATTGTTGTATGAAGTGTCCTGCTATGCTACCAGGGTAACTTTGGATGCATTAGGACAAATGTTCCAGTCTGCCCGTGGTATAATGGCATGGCTTGGTGATTGTGCAAAGATGATTGCTTCAGAAAACCACCCTGTCAAATGGACGAGCCCCGTTGGTCTTCCGGTTGTTCAGCCCTACAAGAAATATAAGAACTACATGATTCGAACTTCACTGCAATGTCTGGCTTTACGACGGGAGGGAGATGCAATTGCACTCCAGCGGCAAAAGGCAGCTTTTCCCCCAAATTTTGTGCACTCTCTTGATAGTTCACACATGATGATGACTGCTATTGCATGCAAAAAGGCTGGTCTTCATTTTGCAGGGGTGCATGACTCGTTTTGGGTGCATGCATGTGATGTTGATAAAATGAATCAGATACTGAGAGAACAATTTGTGGAGCTTTACAGCATGCCAATTCTTGAAAATTTGCTCAAGGAGTTCCAGACATCATTCCCAACACTAGAATTTCCTCCCTGTCCTTCACAAGGGGACTTCGATGTGAGAGAAGTTCTTGCTTCAACCTACTTCTTCAACTAAGCTTAATGGAAAAGTTTTGCACATTAGCACATACGCAGCAAGTCAGTAAGTTCGGCAGTAGCTGAATAGCCAGCCATATTGCTGATACCATGGTGATTTGGTGCCAAGTACGAAATGTGGCATAAGCATCAGAGAAGAGCAACACACCGTTGCAACTGCTGGGAGCCTGGAACAGTATATGGGTTTTTGTCATTGGCAAATCAGTTCCCCAGATTGCCGATTTTGGAGGAAGCTAAAAAGTTCCAAGAAACCTGAGCTGAAACAAAACCCAGTACTTCCTGCTGATGTTTTTAGATTCATGCAGGATCTACTAGGATTTCAGTTCAGGGAATTCACCATGCTACACCCAGCCAATGAAGGCGATTTTGCGACATAACAGGTTATCAACTACAGAATACTGATCGATACTAGTCCTGCTGACCACCCTGACCAATTTGATCATAGGCGACAGTTGAACCATAATTTCACTGTCTATACATGTAACATTATCTTTTGGCCTCTTAGGGCTATAATTATAGCCGATTGCTGTATCGGTGCGGTGACCTGGTAATAATGTGTAGAATAAAGTGCCAAAGAGAAATGTATACTAGTGTACATGATTGCTGGATGCTGTTGATAGCAAGCAACCTGAAAGAGAGAAATGTACATGAAAACGGCTCGATCTTGTCGATTCGTTCAAATATCTGCTTCCTTAGGTTCATTGCCAAAATAA
CDS Sequence
  • >LOC_Os06g44230.1
    ATGCCTCTCCTCCTCTTCCCCATCTCCCCTCCCTGCGTGCCCCCGCCGCGGCCCCGCCTACGCCGCCTCTCCCCGCCGCCGCCCATGGCGGCCGTCGCGCCGCCGTCGCTGAGCACGCCCGTGACCATACTCCCCTCCGTGTCTGTCGCCTTGCCCCCGCTTCCACCCCCTGCTACTGATGACTTCCACTGGCTCGACCTCTTCGCGTTCCTCAACTCCCCCGCGGATTCCTACCAGATTCCTGTGGAGGAGCAGGAGGTGGAGGTGGAGGTGGAGGTGGAGGTGGGGGTGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGCGGGCGAGGAAGGCGGAGCACCGGCGGCTGCGGCAGCGGCAGGTGAAGGCGGAGACGGAGGCGTGGGCGCGGGCCGCCGACGAGTACCGGGAGCTCGAGCGGGAGATGCTCGACCGGAGGCTCGCGCCCGCGCTGCCCTACGTCAAGTCGCTCTTCCTCGGCTGGTTCGAGCCGCTGCGCGACGCCATCGCCCGGGACCAGGAGGTGCAGCGGCGGAAGCGGGTCAAGCACGTCTACGCCAAGTACCTGCTCCTGCTGCCCGCGGACAAGGTGGCCGTCATCGTCATGCACAAGATGATGGGCCTCCTCATGTCGAGCAAGGATGGCGTCGCCAGCGTCCGTGTTGTCCAGGCCGCGCACTGCATCGGCGAGGCTGTTGAACGTGAGTTCAAAGTCCAGACATTCTTTCAAAAGACAAGGAAGAAAAGTGCAGGTGAAAATGATTTGGCATTAGAGAAGGAGCAGGCCAAATGCCGAAAACGTGTTAAGAGTTTGGTTAGGAGGAGAAAGTTGACTGAAGCACAGAAGATTGTGCAACAGGAGATTGAATTGGAAGAATGGGGCACAGAATCCCAAGTGAAGTTAGGGACCCGCTTGATTGAGTTGTTACTGGATTCAGCTTTTGTACAGTCACCAGCTGACCAAACACCAGAGAGTTCTCCAGATATTCGACCAGCATTTAAACACGTACTTCGACAACCTATAGTAGAAAATGGGAGACTGAAGAAAAAGCACTGGGTGATAGAGTGTGACCCTCTTGTGCATGAGGGGTTTGAAAGCACTGCTAGGCACGTGGAGATACCCTATCTTCCTATGCTTGTGACTCCTAAAAAATGGAAGGGCTATGATACAGGTGGGTACCTTTTTCTTCCCTCATATATTATGCGGACACATGGCGTGAAAGATCAAAAGGAAGCCATTAAGAGTGTTCCGAGAAAACAGCTACGGAAAGTATTTGAGGCATTAGATACCCTTGGGAGTACTAAATGGAGGGTGAATAGAAGAGTACATAATGCTGTTGAAACTATTTGGAGTCGAGGTGGGGGCATTGCAGGACTGGTTGATAAGGAAAATATTCCTCTACCTGAACGACCAGAAACGGAGGATCCAGATGAAATACAGAAATGGAAGTGGAGCTTAAAGAAGGCAAAGAAAGCTAATCGTGAACTGCATGCTGAGCGATGCGATACAGAGCTTAAACTATCTGTTGCTCGGAAAATGAGAGAGGAGGATGGATTTTATTATCCCCATAATCTTGATTTTCGTGGCCGTGCATACCCTATGCATGCACATCTAAGCCACCTAGGTTCAGATCTATGCAGAGGTGTTCTAGAGTATGCTGAAGGGCGGCCACTAGGAAAGTCTGGCTTACGCTGGTTAAAGATTCATTTGGCTAACAAATATGGTGGAGGAATTGAAAAGCTTTCTCATGAGGACAAGGTAGCATTTGTGGAGAACCAGTTGCCTGATATATTTGATTCAGCAACTAATCCTGTTGACGGGAATTGCTGGTGGATGAATGCTGAGGATCCTTTTCAATGTTTAGCCGCATGCATGGATCTTTCTGATGCCTTAAAAAGTTCATCACCTCAGTGTGCTGTTTCTCACCTACCTATTCATCAGGATGGTTCATGCAATGGTTTACAACACTATGCTGCTCTTGGAAGAGACTATATGGGTGCAGCTGCTGTCAATTTAGTTCCTGGAGACAAACCTGCAGATATCTACTCAGAGATTGCTGCTAGGGTGCTGGATGTTGTTCGGGAAGACTCGATGGAGGATCCTGCAACTAATCCAACTGCCTCATTAGCAAGGGTTTTAGTCGATCAGGTGGATAGGAAGCTTGTTAAGCAGACAGTGATGACATCAGTTTACGGTGTCACATATATTGGTGCTCGTCAACAGATAACAAAAAGACTACAAGAGAAAGGGCTCATTACAGATGACAAATTGTTGTATGAAGTGTCCTGCTATGCTACCAGGGTAACTTTGGATGCATTAGGACAAATGTTCCAGTCTGCCCGTGGTATAATGGCATGGCTTGGTGATTGTGCAAAGATGATTGCTTCAGAAAACCACCCTGTCAAATGGACGAGCCCCGTTGGTCTTCCGGTTGTTCAGCCCTACAAGAAATATAAGAACTACATGATTCGAACTTCACTGCAATGTCTGGCTTTACGACGGGAGGGAGATGCAATTGCACTCCAGCGGCAAAAGGCAGCTTTTCCCCCAAATTTTGTGCACTCTCTTGATAGTTCACACATGATGATGACTGCTATTGCATGCAAAAAGGCTGGTCTTCATTTTGCAGGGGTGCATGACTCGTTTTGGGTGCATGCATGTGATGTTGATAAAATGAATCAGATACTGAGAGAACAATTTGTGGAGCTTTACAGCATGCCAATTCTTGAAAATTTGCTCAAGGAGTTCCAGACATCATTCCCAACACTAGAATTTCCTCCCTGTCCTTCACAAGGGGACTTCGATGTGAGAGAAGTTCTTGCTTCAACCTACTTCTTCAACTAA
Protein Sequence
  • >LOC_Os06g44230.1
    MPLLLFPISPPCVPPPRPRLRRLSPPPPMAAVAPPSLSTPVTILPSVSVALPPLPPPATDDFHWLDLFAFLNSPADSYQIPVEEQEVEVEVEVEVGVERERERERERERERERARKAEHRRLRQRQVKAETEAWARAADEYRELEREMLDRRLAPALPYVKSLFLGWFEPLRDAIARDQEVQRRKRVKHVYAKYLLLLPADKVAVIVMHKMMGLLMSSKDGVASVRVVQAAHCIGEAVEREFKVQTFFQKTRKKSAGENDLALEKEQAKCRKRVKSLVRRRKLTEAQKIVQQEIELEEWGTESQVKLGTRLIELLLDSAFVQSPADQTPESSPDIRPAFKHVLRQPIVENGRLKKKHWVIECDPLVHEGFESTARHVEIPYLPMLVTPKKWKGYDTGGYLFLPSYIMRTHGVKDQKEAIKSVPRKQLRKVFEALDTLGSTKWRVNRRVHNAVETIWSRGGGIAGLVDKENIPLPERPETEDPDEIQKWKWSLKKAKKANRELHAERCDTELKLSVARKMREEDGFYYPHNLDFRGRAYPMHAHLSHLGSDLCRGVLEYAEGRPLGKSGLRWLKIHLANKYGGGIEKLSHEDKVAFVENQLPDIFDSATNPVDGNCWWMNAEDPFQCLAACMDLSDALKSSSPQCAVSHLPIHQDGSCNGLQHYAALGRDYMGAAAVNLVPGDKPADIYSEIAARVLDVVREDSMEDPATNPTASLARVLVDQVDRKLVKQTVMTSVYGVTYIGARQQITKRLQEKGLITDDKLLYEVSCYATRVTLDALGQMFQSARGIMAWLGDCAKMIASENHPVKWTSPVGLPVVQPYKKYKNYMIRTSLQCLALRREGDAIALQRQKAAFPPNFVHSLDSSHMMMTAIACKKAGLHFAGVHDSFWVHACDVDKMNQILREQFVELYSMPILENLLKEFQTSFPTLEFPPCPSQGDFDVREVLASTYFFN*