Information report for TGW6;OsTGW6
Gene Details
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Functional Descriptions
- Our findings suggest that TGW6 may be useful for further improvements in yield characteristics in most cultivars.
- Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield.
- In sink organs, the Nipponbare TGW6 allele affects the timing of the transition from the syncytial to the cellular phase by controlling IAA supply and limiting cell number and grain length.
- Here, we report the cloning and functional analysis of THOUSAND-GRAIN WEIGHT 6 (TGW6), a gene from the Indian landrace rice Kasalath.
- TGW6 encodes a novel protein with indole-3-acetic acid (IAA)-glucose hydrolase activity.
- Phylogenetic and expression analyses of grain weight genes TaTGW6 and OsTGW6 and investigation of substrate availability indicate TGW6 does not regulate auxin content of grains but may affect pollen development.
- This evidence, combined with our observation that developing wheat grains have undetectable levels of ester IAA in comparison to free IAA and do not express an IAA-glucose synthase suggests that TaTGW6 and OsTGW6 do not regulate grain size via the hydrolysis of IAA-glucose.
- However, a critical comparison of data on TaTGW6 and OsTGW6 with other reports on IAA synthesis in cereal grains requires that this hypothesis be reinvestigated.
- The 1-bp deletion allele of TGW6 cloned from the Indian landrace rice cultivar Kasalath, which has lost function, enhances both grain size and yield.
- TGW6 has been utilized as a target for breeding and genome editing to increase the yield of rice.
Functional Keywords
- yield , grain-length , grain , iaa , grain-weight , auxin , development , pollen , grain-size , pollen-development , IAA , breeding
Literature and News
- Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield . DOI: 10.1038/ng.2612 ; PMID: 23583977
- Bin-based genome-wide association studies reveal superior alleles for improvement of appearance quality using a 4-way MAGIC population in rice . DOI: 10.1016/j.jare.2020.08.001 ; PMID: 33364055
- Reinvestigation of THOUSAND-GRAIN WEIGHT 6 grain weight genes in wheat and rice indicates a role in pollen development rather than regulation of auxin content in grains . DOI: 10.1007/s00122-021-03804-3 ; PMID: 33687498
- Expression, purification and crystallization of TGW6, which limits grain weight in rice . DOI: 10.1016/j.pep.2021.105975 ; PMID: 34536500
Gene Resources
- UniProt: Q69U01
- EMBL: AB513135, AP014962
- EnsemblPlants: Os06t0623700-01
- Gramene: Os06t0623700-01
- Orthologous matrix: MSADRTH
- InterPro: IPR011042, IPR018119
- PANTHER: PTHR10426, PTHR10426:SF95
- SUPFAM: SSF63829
- Gene3D: 2.120.10.30
- SWISS-MODEL: Q69U01
- eggNOG: KOG1520
Sequences
cDNA Sequence
- >LOC_Os06g41850.1
ATGAGAAGCACGGCGAGGCAAGCGGCGACCGCGGCGGCGTTCGCGCTCATTGTCTTCCTCGTGCTGCTCTCGCCGTCCCCTACTGCCGCCGCCACAGCCACAACGAGAATGTTCAAGACCATTGACGCCCGGCGGAGCCAGCATCTGGACCTCGGCGGATCACTGGTCGGCCCGGAGAGCGTCGCGTTCGACGGCAAAGGCCGCGGCCCGTACAGCGGCGTCTCCGACGGCCGCATCATGAGGTGGAACGGCGAGGCCGCTGGCTGGAGCACCTACACGTACAGCCCCAGCTACACGAAAAACAAGTGCGCGGCATCGACTCTCCCCACGGTCCAGACCGAGAGCAAATGCGGCCGCCCGTTAGGCCTACGGTTTCACTACAAAACCGGCAACCTGTACATCGCCGACGCCTACATGGGATTGATGCGAGTTGGTCCAAAAGGCGGGGAGGCAACCGTGCTAGCCATGAAGGCTGATGGCGTGCCACTTCGCTTCACCAATGGGGTGGACATTGATCAGGTTACCGGAGATGTTTATTTCACCGACAGCAGCATGAACTACCAACGATCTCAGCACGAGCAAGTCACGGCGACCAAGGATTCGACCGGACGGCTCATGAAGTATGACCCACGAACTAACCAAGTCACCGTTCTTCAATCCAACATAACCTACCCGAACGGTGTCGCCATGAGCGCTGACCGAACACATCTGATCGTTGCATTGACCGGGCCATGTAAGTTGATGAGGCATTGGATCCGAGGCCCGAAGACTGGCAAATCTGAACCATTTGTTGACCTGCCAGGCTATCCTGATAATGTGAGGCCTGATGGAAAAGGTGGTTATTGGATAGCGCTTCATCGCGAGAAGTATGAGCTTCCCTTTGGTCCGGATAGTCACTTGGTTGCTATGAGGGTTAGTGCTGGTGGGAAGCTGGTTCAACAGATGAGAGGACCAAAGAGCTTGAGGCCAACCGAAGTGATGGAGAGGAAGGATGGCAAAATATACATGGGAAATGTTGAATTGCCGTATGTCGGAGTCGTCAAAAGCAGCTAG
CDS Sequence
- >LOC_Os06g41850.1
ATGAGAAGCACGGCGAGGCAAGCGGCGACCGCGGCGGCGTTCGCGCTCATTGTCTTCCTCGTGCTGCTCTCGCCGTCCCCTACTGCCGCCGCCACAGCCACAACGAGAATGTTCAAGACCATTGACGCCCGGCGGAGCCAGCATCTGGACCTCGGCGGATCACTGGTCGGCCCGGAGAGCGTCGCGTTCGACGGCAAAGGCCGCGGCCCGTACAGCGGCGTCTCCGACGGCCGCATCATGAGGTGGAACGGCGAGGCCGCTGGCTGGAGCACCTACACGTACAGCCCCAGCTACACGAAAAACAAGTGCGCGGCATCGACTCTCCCCACGGTCCAGACCGAGAGCAAATGCGGCCGCCCGTTAGGCCTACGGTTTCACTACAAAACCGGCAACCTGTACATCGCCGACGCCTACATGGGATTGATGCGAGTTGGTCCAAAAGGCGGGGAGGCAACCGTGCTAGCCATGAAGGCTGATGGCGTGCCACTTCGCTTCACCAATGGGGTGGACATTGATCAGGTTACCGGAGATGTTTATTTCACCGACAGCAGCATGAACTACCAACGATCTCAGCACGAGCAAGTCACGGCGACCAAGGATTCGACCGGACGGCTCATGAAGTATGACCCACGAACTAACCAAGTCACCGTTCTTCAATCCAACATAACCTACCCGAACGGTGTCGCCATGAGCGCTGACCGAACACATCTGATCGTTGCATTGACCGGGCCATGTAAGTTGATGAGGCATTGGATCCGAGGCCCGAAGACTGGCAAATCTGAACCATTTGTTGACCTGCCAGGCTATCCTGATAATGTGAGGCCTGATGGAAAAGGTGGTTATTGGATAGCGCTTCATCGCGAGAAGTATGAGCTTCCCTTTGGTCCGGATAGTCACTTGGTTGCTATGAGGGTTAGTGCTGGTGGGAAGCTGGTTCAACAGATGAGAGGACCAAAGAGCTTGAGGCCAACCGAAGTGATGGAGAGGAAGGATGGCAAAATATACATGGGAAATGTTGAATTGCCGTATGTCGGAGTCGTCAAAAGCAGCTAG
Protein Sequence
- >LOC_Os06g41850.1
MRSTARQAATAAAFALIVFLVLLSPSPTAAATATTRMFKTIDARRSQHLDLGGSLVGPESVAFDGKGRGPYSGVSDGRIMRWNGEAAGWSTYTYSPSYTKNKCAASTLPTVQTESKCGRPLGLRFHYKTGNLYIADAYMGLMRVGPKGGEATVLAMKADGVPLRFTNGVDIDQVTGDVYFTDSSMNYQRSQHEQVTATKDSTGRLMKYDPRTNQVTVLQSNITYPNGVAMSADRTHLIVALTGPCKLMRHWIRGPKTGKSEPFVDLPGYPDNVRPDGKGGYWIALHREKYELPFGPDSHLVAMRVSAGGKLVQQMRGPKSLRPTEVMERKDGKIYMGNVELPYVGVVKSS*