Gene Details:

Functional Descriptions:

  • Our findings suggest that TGW6 may be useful for further improvements in yield characteristics in most cultivars.
  • Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield.
  • In sink organs, the Nipponbare TGW6 allele affects the timing of the transition from the syncytial to the cellular phase by controlling IAA supply and limiting cell number and grain length.
  • Here, we report the cloning and functional analysis of THOUSAND-GRAIN WEIGHT 6 (TGW6), a gene from the Indian landrace rice Kasalath.
  • TGW6 encodes a novel protein with indole-3-acetic acid (IAA)-glucose hydrolase activity.
  • Phylogenetic and expression analyses of grain weight genes TaTGW6 and OsTGW6 and investigation of substrate availability indicate TGW6 does not regulate auxin content of grains but may affect pollen development.
  • This evidence, combined with our observation that developing wheat grains have undetectable levels of ester IAA in comparison to free IAA and do not express an IAA-glucose synthase suggests that TaTGW6 and OsTGW6 do not regulate grain size via the hydrolysis of IAA-glucose.
  • However, a critical comparison of data on TaTGW6 and OsTGW6 with other reports on IAA synthesis in cereal grains requires that this hypothesis be reinvestigated.
  • The 1-bp deletion allele of TGW6 cloned from the Indian landrace rice cultivar Kasalath, which has lost function, enhances both grain size and yield.
  • TGW6 has been utilized as a target for breeding and genome editing to increase the yield of rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g41850.1
    ATGAGAAGCACGGCGAGGCAAGCGGCGACCGCGGCGGCGTTCGCGCTCATTGTCTTCCTCGTGCTGCTCTCGCCGTCCCCTACTGCCGCCGCCACAGCCACAACGAGAATGTTCAAGACCATTGACGCCCGGCGGAGCCAGCATCTGGACCTCGGCGGATCACTGGTCGGCCCGGAGAGCGTCGCGTTCGACGGCAAAGGCCGCGGCCCGTACAGCGGCGTCTCCGACGGCCGCATCATGAGGTGGAACGGCGAGGCCGCTGGCTGGAGCACCTACACGTACAGCCCCAGCTACACGAAAAACAAGTGCGCGGCATCGACTCTCCCCACGGTCCAGACCGAGAGCAAATGCGGCCGCCCGTTAGGCCTACGGTTTCACTACAAAACCGGCAACCTGTACATCGCCGACGCCTACATGGGATTGATGCGAGTTGGTCCAAAAGGCGGGGAGGCAACCGTGCTAGCCATGAAGGCTGATGGCGTGCCACTTCGCTTCACCAATGGGGTGGACATTGATCAGGTTACCGGAGATGTTTATTTCACCGACAGCAGCATGAACTACCAACGATCTCAGCACGAGCAAGTCACGGCGACCAAGGATTCGACCGGACGGCTCATGAAGTATGACCCACGAACTAACCAAGTCACCGTTCTTCAATCCAACATAACCTACCCGAACGGTGTCGCCATGAGCGCTGACCGAACACATCTGATCGTTGCATTGACCGGGCCATGTAAGTTGATGAGGCATTGGATCCGAGGCCCGAAGACTGGCAAATCTGAACCATTTGTTGACCTGCCAGGCTATCCTGATAATGTGAGGCCTGATGGAAAAGGTGGTTATTGGATAGCGCTTCATCGCGAGAAGTATGAGCTTCCCTTTGGTCCGGATAGTCACTTGGTTGCTATGAGGGTTAGTGCTGGTGGGAAGCTGGTTCAACAGATGAGAGGACCAAAGAGCTTGAGGCCAACCGAAGTGATGGAGAGGAAGGATGGCAAAATATACATGGGAAATGTTGAATTGCCGTATGTCGGAGTCGTCAAAAGCAGCTAG
CDS Sequence
  • >LOC_Os06g41850.1
    ATGAGAAGCACGGCGAGGCAAGCGGCGACCGCGGCGGCGTTCGCGCTCATTGTCTTCCTCGTGCTGCTCTCGCCGTCCCCTACTGCCGCCGCCACAGCCACAACGAGAATGTTCAAGACCATTGACGCCCGGCGGAGCCAGCATCTGGACCTCGGCGGATCACTGGTCGGCCCGGAGAGCGTCGCGTTCGACGGCAAAGGCCGCGGCCCGTACAGCGGCGTCTCCGACGGCCGCATCATGAGGTGGAACGGCGAGGCCGCTGGCTGGAGCACCTACACGTACAGCCCCAGCTACACGAAAAACAAGTGCGCGGCATCGACTCTCCCCACGGTCCAGACCGAGAGCAAATGCGGCCGCCCGTTAGGCCTACGGTTTCACTACAAAACCGGCAACCTGTACATCGCCGACGCCTACATGGGATTGATGCGAGTTGGTCCAAAAGGCGGGGAGGCAACCGTGCTAGCCATGAAGGCTGATGGCGTGCCACTTCGCTTCACCAATGGGGTGGACATTGATCAGGTTACCGGAGATGTTTATTTCACCGACAGCAGCATGAACTACCAACGATCTCAGCACGAGCAAGTCACGGCGACCAAGGATTCGACCGGACGGCTCATGAAGTATGACCCACGAACTAACCAAGTCACCGTTCTTCAATCCAACATAACCTACCCGAACGGTGTCGCCATGAGCGCTGACCGAACACATCTGATCGTTGCATTGACCGGGCCATGTAAGTTGATGAGGCATTGGATCCGAGGCCCGAAGACTGGCAAATCTGAACCATTTGTTGACCTGCCAGGCTATCCTGATAATGTGAGGCCTGATGGAAAAGGTGGTTATTGGATAGCGCTTCATCGCGAGAAGTATGAGCTTCCCTTTGGTCCGGATAGTCACTTGGTTGCTATGAGGGTTAGTGCTGGTGGGAAGCTGGTTCAACAGATGAGAGGACCAAAGAGCTTGAGGCCAACCGAAGTGATGGAGAGGAAGGATGGCAAAATATACATGGGAAATGTTGAATTGCCGTATGTCGGAGTCGTCAAAAGCAGCTAG
Protein Sequence
  • >LOC_Os06g41850.1
    MRSTARQAATAAAFALIVFLVLLSPSPTAAATATTRMFKTIDARRSQHLDLGGSLVGPESVAFDGKGRGPYSGVSDGRIMRWNGEAAGWSTYTYSPSYTKNKCAASTLPTVQTESKCGRPLGLRFHYKTGNLYIADAYMGLMRVGPKGGEATVLAMKADGVPLRFTNGVDIDQVTGDVYFTDSSMNYQRSQHEQVTATKDSTGRLMKYDPRTNQVTVLQSNITYPNGVAMSADRTHLIVALTGPCKLMRHWIRGPKTGKSEPFVDLPGYPDNVRPDGKGGYWIALHREKYELPFGPDSHLVAMRVSAGGKLVQQMRGPKSLRPTEVMERKDGKIYMGNVELPYVGVVKSS*