Information report for OsbZIP50;OsbZIP74
Gene Details
|
|
Functional Descriptions
- Under normal conditions, OsbZIP74 mRNA encodes a basic leucine-zipper transcription factor with a putative transmembrane domain.
- This study demonstrates that the Oryza sativa (rice) OsbZIP50 transcription factor, an orthologue of Arabidopsis AtbZIP60, is regulated by IRE1-mediated splicing of its RNA.
- OsbZIP50 mRNA is spliced in a similar manner to HAC1 and XBP1 mRNAs; however, this splicing has very different effects on the translation products, a finding that shows the diversity of IRE1-related transcription factors in eukaryotes.
- The activated form of OsbZIP74 has transcriptional activation activity in both yeast cells and Arabidopsis leaf protoplasts.
- Ribonuclease mediates the unconventional splicing of mRNA encoding the transcription factor AtbZIP60 in Arabidopsis, or OsbZIP50 in rice, and thereby transduces signals from stressed ER.
- When treating with ER stress-inducing agents such as tunicamycin and DTT, the conserved double stem-loop structures of OsbZIP74 mRNA are spliced out.
- We also demonstrate that the unconventional splicing of OsbZIP74 mRNA is associated with heat stress and salicylic acid, which is an important plant hormone in systemic acquired resistance against pathogen or parasite.
- Ectopic expression of OsbZIP50 in Arabidopsis significantly increases plant zinc accumulation under control zinc supply, suggesting an altered Zn sensing in OsbZIP50.
Functional Keywords
- transcription-factor , leaf , stem , salicylic-acid , zinc
Literature and News
- Cis-element of the rice PDIL2-3 promoter is responsible for inducing the endoplasmic reticulum stress response . DOI: 10.1016/j.jbiosc.2013.10.023 ; PMID: 24315532
- Genome-wide analysis of basic leucine zipper transcription factor families in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa . DOI: 10.1007/s11741-009-0216-3
- Signal transduction by IRE1-mediated splicing of bZIP50 and other stress sensors in the endoplasmic reticulum stress response of rice . DOI: 10.1111/j.1365-313X.2011.04844.x ; PMID: 22050533
- Conservation of IRE1-regulated bZIP74 mRNA unconventional splicing in rice (Oryza sativa L.) involved in ER stress responses . DOI: 10.1093/mp/ssr115 ; PMID: 22199238
- Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice . DOI: 10.1104/pp.107.112821 ; PMID: 18065552
- Multiple roles of the ER stress sensor IRE1 demonstrated by gene targeting in rice . DOI: 10.1038/srep00944 ; PMID: 23230509
- Rice F-bZIP transcription factors regulate the zinc deficiency response . DOI: 10.1093/jxb/eraa115 ; PMID: 32133499
- Comparative transcriptome profile analysis of rice varieties with different tolerance to zinc deficiency . DOI: 10.1111/plb.13227 ; PMID: 33296551
Gene Resources
- NCBI ID: AK107021
Sequences
cDNA Sequence
- >LOC_Os06g41770.1
ATGACATGGCACATTCAAGCCCTCCCATTGGCCATGCCACGGCACCACCCCGCGTGGGCCCCACCACCTCCCTTCGCCGAAACTTTCCCCTCCTCCTTCCTCCCGAAGCAAAATCCGAGCATCTCCGACGAGTCCGCCATATTTAAACACACCCACGAGCGGCAACCCACCGCGAGAGACAAAAATATCTCCATCGCGATCTCCATGGATGTAGAGTTCTTCGCCGACCTCGACCTCGACGCGCTCCTCGCCTCCTTCTCCTCCTCCGCCGCCGCCGCCGGCTCCGGCGTCTCGGGCCTCTTCGCCCCGTCACCGCCGCACGATGCGGAGGCGGGGTCGCCGGAGTCGGTGAGCTCCCGGCGGCCCAGCCCTTCCCGGGAGGCGGCGCTGTCGGAGATCGAGAGGTTCCTGATGGAGGAGGGCCCCGCGGCGGAGGAGGGGGTCGGCGCGGAGGATTTCTTCGACGCGCTGCTCGTCGACGGCGGGGAGGAGGAGGAGGAAGAAGAGGGGAAGGGGAGTGAGGCGGGGGGAAGCACGGATGGGGATTCCGGGAAGGAGAATGAGGTGGCTACCCCGGACGCGGAGAAGGAGGATGTGGAGGCGGAGGTGGATGGCGATGATCCCATGAGCAAGAAGAAGAGGAGGCAGATGAGAAATAGGGATTCTGCCATGAAATCGAGGGAGAGGAAGAAGATGTATGTTAAGGACCTAGAGACTAAGAGCAAGTATCTAGAGGCCGAGTGTCGTCGCCTCAGCTACGCGCTTCAGTGCTGCGCAGCTGAGAACATGGCGCTGCGCCAGAGCTTGTTGAAGGATAGGCCTGTCGGTGCCGCCACAGCCATGCAGGAGTCTGCCGTACTCACGGAAACCCTGCCGCTGGTTTCCCTGCTTTGGCTCGTGAGCATCGTGTGCCTACTCCCGGTGCCCGGTCTACCCAACCGAAACCCGGTGGCTCGAAGCAGCGCCGGAAGAGATCTCGCGACGGTAACCGGAAAGAAGACAAGCAGTGAACAACAGCTAGAAGAAACATTACTACTCCATGGGAGGCGTTGCAAGGGCTCGAGGGCGAGGATCAAGCTAGATACCGGACCGTTCCGTTTAGCAGCAGCTGCTTGCTAGGCTGCCGCTTCCTTGCAAAGTTGTTTCCAGTCTGTATTGTAGCTAGCAAATCAAATTGGCGTGTCTATCGCCTGTCGTGCTGCCGCTTATCCATGGACTATCTTTTTCTTTTTTTGGTTCCTTCCCCCCTTGTTTTTATAATATCCTCCTATGCATAATGCATTATCCAGGTAGTTTTGAAATCTGGCTTTGTTGAGTTGTTTGCTGGCTTCTCATCTTGTCGGAAGATTTACAGTTGCTCCTACACTCCTACTTGCCAGGCTCTCTGGAAGTGTATGGCAGTAAAAAACAGATATATTGTTCCTCAGCAGTATTTGCCTTGATGTGAGTAAATGAAACAAATAAACAAACAAGCGC
CDS Sequence
- >LOC_Os06g41770.1
ATGACATGGCACATTCAAGCCCTCCCATTGGCCATGCCACGGCACCACCCCGCGTGGGCCCCACCACCTCCCTTCGCCGAAACTTTCCCCTCCTCCTTCCTCCCGAAGCAAAATCCGAGCATCTCCGACGAGTCCGCCATATTTAAACACACCCACGAGCGGCAACCCACCGCGAGAGACAAAAATATCTCCATCGCGATCTCCATGGATGTAGAGTTCTTCGCCGACCTCGACCTCGACGCGCTCCTCGCCTCCTTCTCCTCCTCCGCCGCCGCCGCCGGCTCCGGCGTCTCGGGCCTCTTCGCCCCGTCACCGCCGCACGATGCGGAGGCGGGGTCGCCGGAGTCGGTGAGCTCCCGGCGGCCCAGCCCTTCCCGGGAGGCGGCGCTGTCGGAGATCGAGAGGTTCCTGATGGAGGAGGGCCCCGCGGCGGAGGAGGGGGTCGGCGCGGAGGATTTCTTCGACGCGCTGCTCGTCGACGGCGGGGAGGAGGAGGAGGAAGAAGAGGGGAAGGGGAGTGAGGCGGGGGGAAGCACGGATGGGGATTCCGGGAAGGAGAATGAGGTGGCTACCCCGGACGCGGAGAAGGAGGATGTGGAGGCGGAGGTGGATGGCGATGATCCCATGAGCAAGAAGAAGAGGAGGCAGATGAGAAATAGGGATTCTGCCATGAAATCGAGGGAGAGGAAGAAGATGTATGTTAAGGACCTAGAGACTAAGAGCAAGTATCTAGAGGCCGAGTGTCGTCGCCTCAGCTACGCGCTTCAGTGCTGCGCAGCTGAGAACATGGCGCTGCGCCAGAGCTTGTTGAAGGATAGGCCTGTCGGTGCCGCCACAGCCATGCAGGAGTCTGCCGTACTCACGGAAACCCTGCCGCTGGTTTCCCTGCTTTGGCTCGTGAGCATCGTGTGCCTACTCCCGGTGCCCGGTCTACCCAACCGAAACCCGGTGGCTCGAAGCAGCGCCGGAAGAGATCTCGCGACGGTAACCGGAAAGAAGACAAGCAGTGAACAACAGCTAGAAGAAACATTACTACTCCATGGGAGGCGTTGCAAGGGCTCGAGGGCGAGGATCAAGCTAGATACCGGACCGTTCCGTTTAGCAGCAGCTGCTTGCTAG
Protein Sequence
- >LOC_Os06g41770.1
MTWHIQALPLAMPRHHPAWAPPPPFAETFPSSFLPKQNPSISDESAIFKHTHERQPTARDKNISIAISMDVEFFADLDLDALLASFSSSAAAAGSGVSGLFAPSPPHDAEAGSPESVSSRRPSPSREAALSEIERFLMEEGPAAEEGVGAEDFFDALLVDGGEEEEEEEGKGSEAGGSTDGDSGKENEVATPDAEKEDVEAEVDGDDPMSKKKRRQMRNRDSAMKSRERKKMYVKDLETKSKYLEAECRRLSYALQCCAAENMALRQSLLKDRPVGAATAMQESAVLTETLPLVSLLWLVSIVCLLPVPGLPNRNPVARSSAGRDLATVTGKKTSSEQQLEETLLLHGRRCKGSRARIKLDTGPFRLAAAAC*