Gene Details:
- MSU gene ID: LOC_Os06g41730
- RAPdb gene ID: Os06g0622300
- Gene Symbol: OsARID3
- Genome: MSU7 , IRGSP-1.0
- Species: Oryza sativa
Functional Descriptions:
- Disruption of OsARID3 leads to defective SAM, early seedling lethality, and impaired capacity of in vitro shoot regeneration.
- We conclude that OsARID3 is an AT-rich specific DNA-binding protein and that it plays a major role in SAM development in rice.
- We show that the expression levels of several KNOXI genes and the biosynthetic genes for auxin and cytokinin are significantly altered in the OsARID3 mutant calli.
- Moreover, we determine that auxin concentrations are increased whereas cytokinin levels are decreased in OsARID3 calli.
- Furthermore, our chromatin immunoprecipitation results demonstrate that OsARID3 binds directly to the KNOXI gene OSH71, the auxin biosynthetic genes OsYUC1 and OsYUC6, and the cytokinin biosynthetic genes OsIPT2 and OsIPT7.
- Disruption of OsARID3 leads to a defective SAM, early seedling lethality, and impaired capacity of in<U+00A0>vitro shoot regeneration.
- Moreover, we determine that auxin concentrations are increased, whereas cytokinin levels are decreased, in OsARID3 calli.
- Furthermore, chromatin immunoprecipitation results demonstrate that OsARID3 binds directly to the KNOXI gene OSH71, the auxin biosynthetic genes OsYUC1 and OsYUC6, and the cytokinin biosynthetic genes OsIPT2 and OsIPT7.
Function-related keywords:
- shoot , seedling , development , auxin , cytokinin
Literature:
- OsARID3, an AT-rich Interaction Domain-containing protein, is required for shoot meristem development in rice . DOI: 10.1111/tpj.12927 ; PMID: 26121094
- OsARID3, an AT-rich Interaction Domain-containing protein, is required for shoot meristem development in rice . DOI: 10.1111/tpj.12927 ; PMID: 26121094
Related News:
Gene Resources:
Sequences:
cDNA Sequence
- >LOC_Os06g41730.1
TCAGTTCAAAGCGTTCCCCCTGTTCATTTCGACCTCTCTCTTCCTCTCCCGTCGTCGTCTCCCCCGTCTCCGGCTAGGGTTTCGGCCGCTCGCCGCCCTCGCCTCCGCCTCCGCCTCCGCCGCCGCCGCTCGCCAGGGGGAGCCCTTCTCACCTCACCGCTCCCGTCTCTCTCTCCCCGTCGACCCCGACGGGCTACGGCTCTACACCCTCCCCGCCTCGGCTCGTGTCTGTTCGCCCGCGGGGACGCTGTTTCCGGCGGGCGGAGGCGCGGCCGCGGCGTCGCGGCTCCTCCTCGGAGGCACGGTGCGGGCGACGGCCCTCCTCCTCTCCTTCCCCGACCCTCTCTCTCTATCTCACTCTCTCTGCCCTAGCCTTCTACGCTGTGTGGAGGCGTCGTCGCGCGCGCGCGCCCGGACGGCGAGCATCTGGATTTGAGCCTAATTGAAGCTGAACCCCCATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAAATGGTGCTCTACAAGATGAAGCGCAACATGCAAGGTTGCTTTTGTAGGTGGGGGTTTATGTGATTGACTTGGGCCTGTTCATCCTGATCTTCGTTGCAAACTAGTTACTTTCTGCTTCATTACTCCTGCCATGTTGTCTATTCTGTATTGATTAATTCTAGTTAACTAGATGGTCTAGTTATGTATCTTGTAGTTCGTTAATTGTGATATGTAGCTTAAGTTCAGCTCTATTAGTTTAGAATGTTAATAGCCTGATGCTAAACATGTTCATGGTTTCTTTCTTCCTTTTTTTTTTCTCAACTAAAGAAAAGGTGATAATTTGTAGCATGTAGCTTGTGAGGTTATTCCTAATACTCAGTTCTCATACTACTCAGAACATGAAGACAAGATAGATTCTTGGATGTAACTGTTGCCTTGTGCATTGCTGTTGTATCTGTATAACTGAATTGACAACTGTGCCTGACCATCCCTTGAAACTATTACTGATGGTGTATCAATTGTTTTTAGTGTTATCTTG - >LOC_Os06g41730.2
TCAGTTCAAAGCGTTCCCCCTGTTCATTTCGACCTCTCTCTTCCTCTCCCGTCGTCGTCTCCCCCGTCTCCGGCTAGGGTTTCGGCCGCTCGCCGCCCTCGCCTCCGCCTCCGCCTCCGCCGCCGCCGCTCGCCAGGGGGAGCCCTTCTCACCTCACCGCTCCCGTCTCTCTCTCCCCGTCGACCCCGACGGGCTACGGCTCTACACCCTCCCCGCCTCGGCTCGTGTCTGTTCGCCCGCGGGGACGCTGTTTCCGGCGGGCGGAGGCGCGGCCGCGGCGTCGCGGCTCCTCCTCGGAGGCACGGTGCGGGCGACGGCCCTCCTCCTCTCCTTCCCCGACCCTCTCTCTCTATCTCACTCTCTCTGCCCTAGCCTTCTACGCTGTGTGGAGGCGTCGTCGCGCGCGCGCGCCCGGACGGCGAGCATCTGGATTTGAGCCTAATTGAAGCTGAACCCCCATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAAATGGTGCTCTACAAGATGAAGCGCAACATGCAAGGTTGCTTTTGTAGGTGGGGGTTTATGTGATTGACTTGGGCCTGTTCATCCTGATCTTCGTTGCAAACTAGTTACTTTCTGCTTCATTACTCCTGCCATGTTGTCTATTCTGTATTGATTAATTCTAGTTAACTAGATGGTCTAGTTATGTATCTTGTAGTTCGTTAATTGTGATATGTAGCTTAAGTTCAGCTCTATTAGTTTAGAATGTTAATAGCCTGATGCTAAACATGTTCATGGTTTCTTTCTTCCTTTTTTTTTTCTCAACTAAAGAAAAGGTGATAATTTGTAGCATGTAGCTTGTGAGGTTATTCCTAATACTCAGTTCTCATACTACTCAGAACATGAAGACAAGATAGATTCTTGGATGTAACTGTTGCCTTGTGCATTGCTGTTGTATCTGTATAACTGAATTGACAACTGTGCCTGACCATCCCTTGAAACTATTACTGATGGTGTATCAATTGTTTTTAGTGTTATCTTG
CDS Sequence
- >LOC_Os06g41730.1
ATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAA - >LOC_Os06g41730.2
ATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAA
Protein Sequence
- >LOC_Os06g41730.1
MAQFRSAPVESEAEPGADGPVDEANAVEEEKSGKGEDGIEEAADTVDSVKDSAKPEGMDAKEEAVEQEGGAAADKAKDIPVTSNGKTDAGGNEVAEMKVEKLENGDGHLKADGDSNGDNKGADGEKQLMLASAGEDVEDPVLSKLASNSFMFDYSCGGDDSGTEEEQAAFMKELERFYREKLMEFKPPKFYGEGLNCLKLWRQVTGLGGYDQVTSCKLWRQVGESFKPPKTCTTVSWTFRNFYEKALLEYEKHKIETGEFQVAASNLTERITSESQVGIHASGSGRARRESATRAMQGWHSQRLLGNGEIADPIIKDKGTVSVLKKDKTPKSSGSAKRKRTPTLEDDRIVPYKPDKLQNDSMVLDLGPPADWVKINVRRTKDCYEVYALVPGLLREEVHVQSDPAGRLIVTGEPEQLDNPWGVTPFKKVISLPSRIDPHQTSAVVTLHGQLFVRAPFEQSK* - >LOC_Os06g41730.2
MAQFRSAPVESEAEPGADGPVDEANAVEEEKSGKGEDGIEEAADTVDSVKDSAKPEGMDAKEEAVEQEGGAAADKAKDIPVTSNGKTDAGGNEVAEMKVEKLENGDGHLKADGDSNGDNKGADGEKQLMLASAGEDVEDPVLSKLASNSFMFDYSCGGDDSGTEEEQAAFMKELERFYREKLMEFKPPKFYGEGLNCLKLWRQVTGLGGYDQVTSCKLWRQVGESFKPPKTCTTVSWTFRNFYEKALLEYEKHKIETGEFQVAASNLTERITSESQVGIHASGSGRARRESATRAMQGWHSQRLLGNGEIADPIIKDKGTVSVLKKDKTPKSSGSAKRKRTPTLEDDRIVPYKPDKLQNDSMVLDLGPPADWVKINVRRTKDCYEVYALVPGLLREEVHVQSDPAGRLIVTGEPEQLDNPWGVTPFKKVISLPSRIDPHQTSAVVTLHGQLFVRAPFEQSK*