Gene Details:

Functional Descriptions:

  • Disruption of OsARID3 leads to defective SAM, early seedling lethality, and impaired capacity of in vitro shoot regeneration.
  • We conclude that OsARID3 is an AT-rich specific DNA-binding protein and that it plays a major role in SAM development in rice.
  • We show that the expression levels of several KNOXI genes and the biosynthetic genes for auxin and cytokinin are significantly altered in the OsARID3 mutant calli.
  • Moreover, we determine that auxin concentrations are increased whereas cytokinin levels are decreased in OsARID3 calli.
  • Furthermore, our chromatin immunoprecipitation results demonstrate that OsARID3 binds directly to the KNOXI gene OSH71, the auxin biosynthetic genes OsYUC1 and OsYUC6, and the cytokinin biosynthetic genes OsIPT2 and OsIPT7.
  • Disruption of OsARID3 leads to a defective SAM, early seedling lethality, and impaired capacity of in<U+00A0>vitro shoot regeneration.
  • Moreover, we determine that auxin concentrations are increased, whereas cytokinin levels are decreased, in OsARID3 calli.
  • Furthermore, chromatin immunoprecipitation results demonstrate that OsARID3 binds directly to the KNOXI gene OSH71, the auxin biosynthetic genes OsYUC1 and OsYUC6, and the cytokinin biosynthetic genes OsIPT2 and OsIPT7.

Literature:

Gene Resources:

  • NCBI ID:
  • UniProt accessions:

Sequences:

cDNA Sequence
  • >LOC_Os06g41730.1
    TCAGTTCAAAGCGTTCCCCCTGTTCATTTCGACCTCTCTCTTCCTCTCCCGTCGTCGTCTCCCCCGTCTCCGGCTAGGGTTTCGGCCGCTCGCCGCCCTCGCCTCCGCCTCCGCCTCCGCCGCCGCCGCTCGCCAGGGGGAGCCCTTCTCACCTCACCGCTCCCGTCTCTCTCTCCCCGTCGACCCCGACGGGCTACGGCTCTACACCCTCCCCGCCTCGGCTCGTGTCTGTTCGCCCGCGGGGACGCTGTTTCCGGCGGGCGGAGGCGCGGCCGCGGCGTCGCGGCTCCTCCTCGGAGGCACGGTGCGGGCGACGGCCCTCCTCCTCTCCTTCCCCGACCCTCTCTCTCTATCTCACTCTCTCTGCCCTAGCCTTCTACGCTGTGTGGAGGCGTCGTCGCGCGCGCGCGCCCGGACGGCGAGCATCTGGATTTGAGCCTAATTGAAGCTGAACCCCCATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAAATGGTGCTCTACAAGATGAAGCGCAACATGCAAGGTTGCTTTTGTAGGTGGGGGTTTATGTGATTGACTTGGGCCTGTTCATCCTGATCTTCGTTGCAAACTAGTTACTTTCTGCTTCATTACTCCTGCCATGTTGTCTATTCTGTATTGATTAATTCTAGTTAACTAGATGGTCTAGTTATGTATCTTGTAGTTCGTTAATTGTGATATGTAGCTTAAGTTCAGCTCTATTAGTTTAGAATGTTAATAGCCTGATGCTAAACATGTTCATGGTTTCTTTCTTCCTTTTTTTTTTCTCAACTAAAGAAAAGGTGATAATTTGTAGCATGTAGCTTGTGAGGTTATTCCTAATACTCAGTTCTCATACTACTCAGAACATGAAGACAAGATAGATTCTTGGATGTAACTGTTGCCTTGTGCATTGCTGTTGTATCTGTATAACTGAATTGACAACTGTGCCTGACCATCCCTTGAAACTATTACTGATGGTGTATCAATTGTTTTTAGTGTTATCTTG
  • >LOC_Os06g41730.2
    TCAGTTCAAAGCGTTCCCCCTGTTCATTTCGACCTCTCTCTTCCTCTCCCGTCGTCGTCTCCCCCGTCTCCGGCTAGGGTTTCGGCCGCTCGCCGCCCTCGCCTCCGCCTCCGCCTCCGCCGCCGCCGCTCGCCAGGGGGAGCCCTTCTCACCTCACCGCTCCCGTCTCTCTCTCCCCGTCGACCCCGACGGGCTACGGCTCTACACCCTCCCCGCCTCGGCTCGTGTCTGTTCGCCCGCGGGGACGCTGTTTCCGGCGGGCGGAGGCGCGGCCGCGGCGTCGCGGCTCCTCCTCGGAGGCACGGTGCGGGCGACGGCCCTCCTCCTCTCCTTCCCCGACCCTCTCTCTCTATCTCACTCTCTCTGCCCTAGCCTTCTACGCTGTGTGGAGGCGTCGTCGCGCGCGCGCGCCCGGACGGCGAGCATCTGGATTTGAGCCTAATTGAAGCTGAACCCCCATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAAATGGTGCTCTACAAGATGAAGCGCAACATGCAAGGTTGCTTTTGTAGGTGGGGGTTTATGTGATTGACTTGGGCCTGTTCATCCTGATCTTCGTTGCAAACTAGTTACTTTCTGCTTCATTACTCCTGCCATGTTGTCTATTCTGTATTGATTAATTCTAGTTAACTAGATGGTCTAGTTATGTATCTTGTAGTTCGTTAATTGTGATATGTAGCTTAAGTTCAGCTCTATTAGTTTAGAATGTTAATAGCCTGATGCTAAACATGTTCATGGTTTCTTTCTTCCTTTTTTTTTTCTCAACTAAAGAAAAGGTGATAATTTGTAGCATGTAGCTTGTGAGGTTATTCCTAATACTCAGTTCTCATACTACTCAGAACATGAAGACAAGATAGATTCTTGGATGTAACTGTTGCCTTGTGCATTGCTGTTGTATCTGTATAACTGAATTGACAACTGTGCCTGACCATCCCTTGAAACTATTACTGATGGTGTATCAATTGTTTTTAGTGTTATCTTG
CDS Sequence
  • >LOC_Os06g41730.1
    ATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAA
  • >LOC_Os06g41730.2
    ATGGCCCAGTTTAGGTCTGCGCCTGTGGAAAGCGAGGCGGAGCCAGGCGCCGATGGCCCTGTGGATGAAGCCAATGCTGTGGAAGAGGAGAAATCTGGTAAGGGTGAGGATGGCATTGAGGAGGCTGCCGATACCGTTGATTCCGTGAAGGATTCAGCAAAGCCTGAAGGCATGGATGCAAAGGAGGAGGCGGTGGAGCAGGAGGGTGGGGCCGCAGCGGATAAGGCAAAGGATATTCCTGTGACTAGTAACGGTAAGACGGATGCTGGCGGAAATGAGGTGGCTGAAATGAAGGTTGAAAAATTGGAGAATGGTGATGGGCATTTGAAGGCGGACGGCGATAGCAATGGTGATAACAAGGGTGCTGATGGTGAGAAACAGCTTATGTTAGCGTCAGCTGGGGAGGACGTGGAGGACCCGGTGCTGTCCAAGCTTGCAAGCAACTCCTTCATGTTTGATTACAGCTGTGGTGGTGATGACTCGGGGACTGAGGAGGAGCAGGCTGCTTTCATGAAGGAACTTGAGCGGTTTTACAGGGAGAAGCTTATGGAATTCAAGCCCCCGAAGTTCTATGGTGAAGGGTTGAATTGCCTCAAGTTGTGGAGACAAGTGACTGGGTTGGGTGGCTATGATCAGGTGACATCGTGCAAACTGTGGCGGCAAGTGGGAGAGTCTTTCAAACCTCCGAAGACATGCACAACTGTTTCATGGACCTTCCGTAACTTCTATGAGAAGGCACTCCTTGAATATGAGAAACACAAAATCGAAACGGGCGAGTTTCAAGTTGCTGCATCTAATTTAACAGAGAGGATTACTTCTGAAAGTCAGGTTGGCATCCATGCTTCTGGTTCTGGAAGAGCTAGAAGAGAATCTGCAACCCGAGCTATGCAAGGCTGGCACTCTCAGCGCTTGCTTGGCAATGGTGAAATTGCTGATCCAATAATTAAAGATAAAGGGACGGTGTCTGTCTTAAAAAAGGATAAAACTCCTAAAAGCAGCGGTTCTGCCAAGAGGAAAAGGACACCGACCTTGGAAGATGACAGGATTGTACCATATAAACCTGACAAGCTACAAAATGACTCAATGGTTCTTGACCTTGGCCCTCCAGCTGATTGGGTAAAGATAAACGTTCGGAGAACCAAAGATTGCTATGAAGTCTATGCATTGGTTCCTGGCCTTCTGCGTGAGGAGGTACATGTTCAGTCAGATCCTGCTGGCCGTTTGATAGTTACTGGAGAGCCTGAGCAACTAGACAATCCTTGGGGTGTTACTCCATTCAAGAAGGTTATTAGTTTGCCCTCCCGCATTGATCCTCATCAAACTTCGGCAGTTGTCACTCTCCATGGACAGCTATTTGTCCGTGCACCATTCGAGCAGTCAAAGTAA
Protein Sequence
  • >LOC_Os06g41730.1
    MAQFRSAPVESEAEPGADGPVDEANAVEEEKSGKGEDGIEEAADTVDSVKDSAKPEGMDAKEEAVEQEGGAAADKAKDIPVTSNGKTDAGGNEVAEMKVEKLENGDGHLKADGDSNGDNKGADGEKQLMLASAGEDVEDPVLSKLASNSFMFDYSCGGDDSGTEEEQAAFMKELERFYREKLMEFKPPKFYGEGLNCLKLWRQVTGLGGYDQVTSCKLWRQVGESFKPPKTCTTVSWTFRNFYEKALLEYEKHKIETGEFQVAASNLTERITSESQVGIHASGSGRARRESATRAMQGWHSQRLLGNGEIADPIIKDKGTVSVLKKDKTPKSSGSAKRKRTPTLEDDRIVPYKPDKLQNDSMVLDLGPPADWVKINVRRTKDCYEVYALVPGLLREEVHVQSDPAGRLIVTGEPEQLDNPWGVTPFKKVISLPSRIDPHQTSAVVTLHGQLFVRAPFEQSK*
  • >LOC_Os06g41730.2
    MAQFRSAPVESEAEPGADGPVDEANAVEEEKSGKGEDGIEEAADTVDSVKDSAKPEGMDAKEEAVEQEGGAAADKAKDIPVTSNGKTDAGGNEVAEMKVEKLENGDGHLKADGDSNGDNKGADGEKQLMLASAGEDVEDPVLSKLASNSFMFDYSCGGDDSGTEEEQAAFMKELERFYREKLMEFKPPKFYGEGLNCLKLWRQVTGLGGYDQVTSCKLWRQVGESFKPPKTCTTVSWTFRNFYEKALLEYEKHKIETGEFQVAASNLTERITSESQVGIHASGSGRARRESATRAMQGWHSQRLLGNGEIADPIIKDKGTVSVLKKDKTPKSSGSAKRKRTPTLEDDRIVPYKPDKLQNDSMVLDLGPPADWVKINVRRTKDCYEVYALVPGLLREEVHVQSDPAGRLIVTGEPEQLDNPWGVTPFKKVISLPSRIDPHQTSAVVTLHGQLFVRAPFEQSK*