Gene Details:

Functional Descriptions:

  • Based on map-based cloning and sequence analysis, we identified a 1,459-bp deletion in an adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene, OsABCG15, causing abnormal anthers and male sterility.
  • Consistently, histological analysis showed that OsABCG15 mutants developed obvious abnormality in postmeiotic tapetum degeneration, leading to rapid degredation of young microspores.
  • Expression analysis showed that OsABCG15 is expressed specifically in developmental anthers from stage 8 (meiosis II stage) to stage 10 (late microspore stage).
  • Two genes CYP704B2 and WDA1, involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine, were downregulated in OsABCG15 mutant, suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther locules.
  • The ATP-binding cassette transporter OsABCG15 is required for anther development and pollen fertility in rice.
  • The results suggest that OsABCG15 plays a critical role in exine formation and pollen development, similar to the homologous gene of AtABCG26 in Arabidopsis.
  • OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice.
  • An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development in rice.
  • This OsABCG15 mutant failed to produce any viable pollen and was completely male sterile.
  • Histological analysis indicated that OsABCG15 exhibited an undeveloped anther cuticle, enlarged middle layer, abnormal Ubisch body development, tapetum degeneration with a falling apart style, and collapsed pollen grains without detectable exine.
  • Our results suggested that OsABCG15 played an essential role in the formation of the rice anther cuticle and pollen exine.
  • OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g40550.1
    CTCTGAACCTGTCACTCTTTTTTTCTGTGTCTCTTGGAGGAGATGATGGAGATCAGCAGCAATGAGGAGATGATGGAGATGGCCATTGTTGAGCAGCTGCCTCCTTCCTCTCATCATCTCAATGGTGGCAGTGTTGAGGTTGACATGGAGGAGGATCATGTGTGGCCAACCAAAGATGGCCCTCTCCCTATATTCCTTAAGTTTGAGAATGTGGAGTACAAGGTGAAGCTGACTCCAAAGAACCCACTAACAGCTGCAAGGGTGGCATTTGCATCTCACAAGAGCACTGAGGATCAGGGCAGCTGCAAGCACATCCTCAAGGGGATAGGAGGCAGTGTTGACCCTGGTGAGATCCTGGCACTGATGGGCCCATCTGGCAGTGGCAAGACCACGCTGCTCAAGATACTTGGAGGCAGGCTCAGTGGTGGTGTCAAGGGCCAGATCACCTACAATGACACCCCCTACAGTCCCTGCCTCAAAAGAAGGATTGGATTTGTGACACAGGACGACGTCCTTTTCCCTCAGTTGACAGTGGAGGAGACCCTTGTGTTCGCTGCCTTCTTGAGACTCCCCGCTCGCATGTCAAAGCAGCAGAAGCGCGACAGAGTTGACGCCATCATAACTGAGCTAAATCTAGAGAGGTGCCGGCACACCAAGATCGGCGGCGCGTTCGTGCGCGGCGTGTCGGGCGGCGAGAGGAAGCGGACGAGCATCGGGTACGAGATCCTCGTCGACCCGTCGCTGCTGCTCCTCGACGAGCCCACCTCGGGCCTCGACTCCACGTCGGCGGCGAAGCTCCTCGTCGTGCTCCGCCGCCTCGCCAGGTCGGCGGCCCGCCGGACGGTGATCACCACCATCCACCAGCCCTCCAGCCGGATGTTCCACATGTTCGACAAGCTGCTGCTCGTCGCCGAGGGCCACGCCATCTACCACGGCGGCGCCCGCGGCTGCATGCGCCACTTCGCCGCCCTCGGCTTCTCCCCCGGCATCGCCATGAACCCCGCCGAGTTCCTCCTCGACCTCGCCACCGGCAACCTCGACGGCATCTCCTCCCCCGCCTCCCTCCTCCTCCCCTCCGCCGCCGCCGCCTCGCCGGACTCCCCCGAGTTCAGGTCCCACGTCATCAAGTACTTGCAGGCGAGGCACAGGGCGGCCGGCGAGGAGGAGGCGGCGGCCGCGGCGGCGAGGGAGGGCGGCGGCGGAGGTGGCGCGGGGAGGGATGAGGCGGCGAAGCAGCTGAGAATGGCGGTGAGGATGAGGAAGGATAGGCGGGGCGGCATCGGCTGGCTGGAGCAGTTCACCGTGCTGTCGCGGCGCACGTTCCGGGAGCGCGCCGCCGACTACCTCGACAAGATGCGCCTCGCCCAGTCCGTCGGCGTCGCCCTCCTCCTCGGCCTCCTCTGGTGGAAGTCCCAGACCTCCAACGAGGCCCAGCTCCGTGACCAAGTGGGGTTGATATTCTACATCTGCATATTCTGGACGTCGTCGTCGCTGTTCGGGTCGGTGTACGTGTTCCCGTTCGAGAAGCTGTACCTGGTGAAGGAGAGGAAGGCGGACATGTACAGGCTGAGCGCCTACTACGCGAGCAGCACGGTGTGCGACGCGGTGCCGCACGTCGTGTACCCGGTGCTCTTCACGGCCATCCTCTACTTCATGGCCGACCTGCGCCGCACCGTGCCCTGCTTCTGCCTCACCCTCCTCGCCACCCTCCTCATCGTCCTCACCAGCCAGGGCACCGGCGAGCTGCTCGGCGCCGCCATCCTCAGCGTCAAGCGCGCCGGCGTCATGGCCTCGCTCGTCCTCATGCTCTTCCTCCTCACCGGCGGCTACTACGTCCAGCATATACCGAAGTTCATAAGGTGGCTCAAGTACGTGTCGTTCATGCACTACGGGTTCAACCTGCTGCTGAAGGCGCAGTACCACGGCCACCTGACCTACAACTGCGGCAGCCGCGGCGGCTGCCAGCGGCTGCAGTCCTCGCCGTCGTTCGGCACCGTCGACCTCGACGGCGGCATGCGCGAGGTCTGGATCCTCCTCGCCATGGCCGTCGCCTACCGCCTCCTCGCCTACCTCTGCCTCCGCAAGCGGATCAGCCTCATGCCCTTGTAGTCCATCGATCTGTCAGATCAGGATCAGTTGGTTCGGGGAAGAAGACGACTGATGTGGTAGTGAATTGCAATTGCGATTGATGATGGTGAATGGGCCATTGTTGGTTTGGGTGCTGTGAATTGTTTCAAACTTGAGTTCTTTTCTTTATTTGAGAAGTATTTGAGGCTTGTCTGCTTAGCAAGTTGGGAAATAATCAACCCCATCATTTTAGCATTCAGCTTGTAAGCATAAGCGAAGATTTATTTAAAACTTATTTTTAAAGTTAATTTTAGGTATCTTTTCGTAGTTTA
CDS Sequence
  • >LOC_Os06g40550.1
    ATGATGGAGATCAGCAGCAATGAGGAGATGATGGAGATGGCCATTGTTGAGCAGCTGCCTCCTTCCTCTCATCATCTCAATGGTGGCAGTGTTGAGGTTGACATGGAGGAGGATCATGTGTGGCCAACCAAAGATGGCCCTCTCCCTATATTCCTTAAGTTTGAGAATGTGGAGTACAAGGTGAAGCTGACTCCAAAGAACCCACTAACAGCTGCAAGGGTGGCATTTGCATCTCACAAGAGCACTGAGGATCAGGGCAGCTGCAAGCACATCCTCAAGGGGATAGGAGGCAGTGTTGACCCTGGTGAGATCCTGGCACTGATGGGCCCATCTGGCAGTGGCAAGACCACGCTGCTCAAGATACTTGGAGGCAGGCTCAGTGGTGGTGTCAAGGGCCAGATCACCTACAATGACACCCCCTACAGTCCCTGCCTCAAAAGAAGGATTGGATTTGTGACACAGGACGACGTCCTTTTCCCTCAGTTGACAGTGGAGGAGACCCTTGTGTTCGCTGCCTTCTTGAGACTCCCCGCTCGCATGTCAAAGCAGCAGAAGCGCGACAGAGTTGACGCCATCATAACTGAGCTAAATCTAGAGAGGTGCCGGCACACCAAGATCGGCGGCGCGTTCGTGCGCGGCGTGTCGGGCGGCGAGAGGAAGCGGACGAGCATCGGGTACGAGATCCTCGTCGACCCGTCGCTGCTGCTCCTCGACGAGCCCACCTCGGGCCTCGACTCCACGTCGGCGGCGAAGCTCCTCGTCGTGCTCCGCCGCCTCGCCAGGTCGGCGGCCCGCCGGACGGTGATCACCACCATCCACCAGCCCTCCAGCCGGATGTTCCACATGTTCGACAAGCTGCTGCTCGTCGCCGAGGGCCACGCCATCTACCACGGCGGCGCCCGCGGCTGCATGCGCCACTTCGCCGCCCTCGGCTTCTCCCCCGGCATCGCCATGAACCCCGCCGAGTTCCTCCTCGACCTCGCCACCGGCAACCTCGACGGCATCTCCTCCCCCGCCTCCCTCCTCCTCCCCTCCGCCGCCGCCGCCTCGCCGGACTCCCCCGAGTTCAGGTCCCACGTCATCAAGTACTTGCAGGCGAGGCACAGGGCGGCCGGCGAGGAGGAGGCGGCGGCCGCGGCGGCGAGGGAGGGCGGCGGCGGAGGTGGCGCGGGGAGGGATGAGGCGGCGAAGCAGCTGAGAATGGCGGTGAGGATGAGGAAGGATAGGCGGGGCGGCATCGGCTGGCTGGAGCAGTTCACCGTGCTGTCGCGGCGCACGTTCCGGGAGCGCGCCGCCGACTACCTCGACAAGATGCGCCTCGCCCAGTCCGTCGGCGTCGCCCTCCTCCTCGGCCTCCTCTGGTGGAAGTCCCAGACCTCCAACGAGGCCCAGCTCCGTGACCAAGTGGGGTTGATATTCTACATCTGCATATTCTGGACGTCGTCGTCGCTGTTCGGGTCGGTGTACGTGTTCCCGTTCGAGAAGCTGTACCTGGTGAAGGAGAGGAAGGCGGACATGTACAGGCTGAGCGCCTACTACGCGAGCAGCACGGTGTGCGACGCGGTGCCGCACGTCGTGTACCCGGTGCTCTTCACGGCCATCCTCTACTTCATGGCCGACCTGCGCCGCACCGTGCCCTGCTTCTGCCTCACCCTCCTCGCCACCCTCCTCATCGTCCTCACCAGCCAGGGCACCGGCGAGCTGCTCGGCGCCGCCATCCTCAGCGTCAAGCGCGCCGGCGTCATGGCCTCGCTCGTCCTCATGCTCTTCCTCCTCACCGGCGGCTACTACGTCCAGCATATACCGAAGTTCATAAGGTGGCTCAAGTACGTGTCGTTCATGCACTACGGGTTCAACCTGCTGCTGAAGGCGCAGTACCACGGCCACCTGACCTACAACTGCGGCAGCCGCGGCGGCTGCCAGCGGCTGCAGTCCTCGCCGTCGTTCGGCACCGTCGACCTCGACGGCGGCATGCGCGAGGTCTGGATCCTCCTCGCCATGGCCGTCGCCTACCGCCTCCTCGCCTACCTCTGCCTCCGCAAGCGGATCAGCCTCATGCCCTTGTAG
Protein Sequence
  • >LOC_Os06g40550.1
    MMEISSNEEMMEMAIVEQLPPSSHHLNGGSVEVDMEEDHVWPTKDGPLPIFLKFENVEYKVKLTPKNPLTAARVAFASHKSTEDQGSCKHILKGIGGSVDPGEILALMGPSGSGKTTLLKILGGRLSGGVKGQITYNDTPYSPCLKRRIGFVTQDDVLFPQLTVEETLVFAAFLRLPARMSKQQKRDRVDAIITELNLERCRHTKIGGAFVRGVSGGERKRTSIGYEILVDPSLLLLDEPTSGLDSTSAAKLLVVLRRLARSAARRTVITTIHQPSSRMFHMFDKLLLVAEGHAIYHGGARGCMRHFAALGFSPGIAMNPAEFLLDLATGNLDGISSPASLLLPSAAAASPDSPEFRSHVIKYLQARHRAAGEEEAAAAAAREGGGGGGAGRDEAAKQLRMAVRMRKDRRGGIGWLEQFTVLSRRTFRERAADYLDKMRLAQSVGVALLLGLLWWKSQTSNEAQLRDQVGLIFYICIFWTSSSLFGSVYVFPFEKLYLVKERKADMYRLSAYYASSTVCDAVPHVVYPVLFTAILYFMADLRRTVPCFCLTLLATLLIVLTSQGTGELLGAAILSVKRAGVMASLVLMLFLLTGGYYVQHIPKFIRWLKYVSFMHYGFNLLLKAQYHGHLTYNCGSRGGCQRLQSSPSFGTVDLDGGMREVWILLAMAVAYRLLAYLCLRKRISLMPL*