Gene Details:

Functional Descriptions:

  • An activation-tagged line, OsAt10-D1, shows a 60% reduction in matrix polysaccharide-bound FA and an approximately 300% increase in p-CA in young leaf tissue but no discernible phenotypic alterations in vegetative development, lignin content, or lignin composition.
  • Biomass from OsAt10-D1 exhibits a 20% to 40% increase in saccharification yield depending on the assay.
  • Thus, OsAt10 is an attractive target for improving grass cell wall quality for fuel and animal feed.
  • Overexpression of a BAHD acyltransferase, OsAt10, alters rice cell wall hydroxycinnamic acid content and saccharification.
  • Here we show that overexpression of the rice OsAt10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0 plants under greenhouse conditions.
  • Overexpression of OsAt10 (a Mitchell clade BAHD acyl transferase) in rice, has previously been shown to increase p-CA content in AX in leaves and stems, leading to increased cell wall digestibility, potentially associated with a concomitant decrease in FA content.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >LOC_Os06g39390.1
    GCACACAGTTAGCTCTAGCTTTGCTTGATTTGCAGCAGCAGCAGCAGCAGCAGCAGCTAGCTTGCATCCATGGGCGTCTTCGCCGTCACCAAGGTGTCCGAGGGCCCCGTCCGGCCGTCCGCAGCGACGCCGTCGGAGACGCTGCCGCTCGCCTGGGTCGACCGCTACCCGACGCACCGCGGCCTCGTCGAGTCCGTGCACATCTACCTCCGCCGCGACGACGCCGCCGTCGAGGCGCCGTGCGCCGACGGCGGCGTCATCGTCGAGGGAAAGAAGAAGAATAATAAGCCGGCGGCGGCGGTGGTGCGCGGCGCGCTGGCGGACGCGCTGGTGCACTACTACCCGTTCGCGGGGCGGATCGTGGAGGACGAGCGGTCGCCGGGGCGGCCTGCCGTGCTGTGCTCCGGCGAGGGCGTCTACTTCGTGGAGGCCGCCGCCAACTGCACCCTCGCCGACGTCAACCACCTGGAGCGGCCGCTGCTGCTGTCCAAGGAGGACCTCGTGCCGTGCCCGACGCCGGAGCAGTGGCCCGTCGAGCCGCACAACAGCCTCGCCATGATCCAGGTGACGACGTTCACCTGCGGCGGCTTCGTGATCGGGCTGCGCACCAACCACGCGGTGGCGGACGGCACCGGCGCCGCCCAGTTCATGAACGCCGTCGGCGACCTCGCCCGCGGCCTCCCGGAGCCGCGGGTGAAGCCGATCTGGGCGCGCGACCGCTTCCCGGACCCGGACATCAAGCCCGGCCCGCTGCCGGAGCTCCCCGTGCTGCCGCTCCAGTACATCGCCTTCGACTTCCCCGCCGCCTACCTCGGCAAGCTCAAGGCGCAGTACGCCGCCACCGCCGGCGCCAGCAAGATCTGCTCCGCCTTCGACATCGTCATCGCCAAGCTCTGGCAGTGCCGGACGCGCGCCATCGCCGCCGACCCCGCCGCGGCCGTCAAGCTCTGCTTCTTCGCCAGCGCCCGCCAGGTGCTCGGCCTGGAGACCGGCTACTGGGGCAACGCCATCTTCCCGGTGAAGGTGTCCGCGGCGGCGGGGGAGGTGGCGGCGTCGTCGGTGATCGAGCTCGTCGGCGTGGTCCGGGAGGCGAAGCGGCGGATGGCCGGCGAGTGCCTGCGCTGGGCGGAGGGGCGCACCGGCGGCGCCGACCCGTTCCAGATGACGTTCGACTACGAGTCCGTGTACGTGTCGGACTGGAGCAAGCTCGGGTTCAACGACGTCGACTACGGGTACGGCGCGCCGTCGGCGGCGGGGCCGCTGGTGAACTGCGACCTCATCTCGTCGGTGATCGTCATGCGGGCGCCGGCGCCGCTCGCCGGCACGCGGCTGCTGGCGAGCTGCGTCACCAAGGAGCACGCCGACGACTTCGCCGCCAGGATGAGGGAGGATCTCGTCTAATATACCATGGCCGCCCCCAATAACATTATTAGTCACGTACAATATTGTCACTGAATATTAATTTGTTCGTGTATATCTATTGTGGTATGTATTTTTTTTTTCATAGGAAAAAAGTAGTAGTACGACAATAGGTAGCTGGGAGCTACCTAATATCGACCTCTGGTTTGAGAGTAAGTGAGCGAGCGAGAGATGTAAACCACCTCAGTTTTTACCGTGCTTTGTGACATGCGTGGTACAGTACTGGTACTATTAATCATTGGCCGTGAAAAATTATCTACCCATATGCCACTGTATTTATGGTTGTTTGTGGAGGATTACGTATACTAA
CDS Sequence
  • >LOC_Os06g39390.1
    ATGGGCGTCTTCGCCGTCACCAAGGTGTCCGAGGGCCCCGTCCGGCCGTCCGCAGCGACGCCGTCGGAGACGCTGCCGCTCGCCTGGGTCGACCGCTACCCGACGCACCGCGGCCTCGTCGAGTCCGTGCACATCTACCTCCGCCGCGACGACGCCGCCGTCGAGGCGCCGTGCGCCGACGGCGGCGTCATCGTCGAGGGAAAGAAGAAGAATAATAAGCCGGCGGCGGCGGTGGTGCGCGGCGCGCTGGCGGACGCGCTGGTGCACTACTACCCGTTCGCGGGGCGGATCGTGGAGGACGAGCGGTCGCCGGGGCGGCCTGCCGTGCTGTGCTCCGGCGAGGGCGTCTACTTCGTGGAGGCCGCCGCCAACTGCACCCTCGCCGACGTCAACCACCTGGAGCGGCCGCTGCTGCTGTCCAAGGAGGACCTCGTGCCGTGCCCGACGCCGGAGCAGTGGCCCGTCGAGCCGCACAACAGCCTCGCCATGATCCAGGTGACGACGTTCACCTGCGGCGGCTTCGTGATCGGGCTGCGCACCAACCACGCGGTGGCGGACGGCACCGGCGCCGCCCAGTTCATGAACGCCGTCGGCGACCTCGCCCGCGGCCTCCCGGAGCCGCGGGTGAAGCCGATCTGGGCGCGCGACCGCTTCCCGGACCCGGACATCAAGCCCGGCCCGCTGCCGGAGCTCCCCGTGCTGCCGCTCCAGTACATCGCCTTCGACTTCCCCGCCGCCTACCTCGGCAAGCTCAAGGCGCAGTACGCCGCCACCGCCGGCGCCAGCAAGATCTGCTCCGCCTTCGACATCGTCATCGCCAAGCTCTGGCAGTGCCGGACGCGCGCCATCGCCGCCGACCCCGCCGCGGCCGTCAAGCTCTGCTTCTTCGCCAGCGCCCGCCAGGTGCTCGGCCTGGAGACCGGCTACTGGGGCAACGCCATCTTCCCGGTGAAGGTGTCCGCGGCGGCGGGGGAGGTGGCGGCGTCGTCGGTGATCGAGCTCGTCGGCGTGGTCCGGGAGGCGAAGCGGCGGATGGCCGGCGAGTGCCTGCGCTGGGCGGAGGGGCGCACCGGCGGCGCCGACCCGTTCCAGATGACGTTCGACTACGAGTCCGTGTACGTGTCGGACTGGAGCAAGCTCGGGTTCAACGACGTCGACTACGGGTACGGCGCGCCGTCGGCGGCGGGGCCGCTGGTGAACTGCGACCTCATCTCGTCGGTGATCGTCATGCGGGCGCCGGCGCCGCTCGCCGGCACGCGGCTGCTGGCGAGCTGCGTCACCAAGGAGCACGCCGACGACTTCGCCGCCAGGATGAGGGAGGATCTCGTCTAA
Protein Sequence
  • >LOC_Os06g39390.1
    MGVFAVTKVSEGPVRPSAATPSETLPLAWVDRYPTHRGLVESVHIYLRRDDAAVEAPCADGGVIVEGKKKNNKPAAAVVRGALADALVHYYPFAGRIVEDERSPGRPAVLCSGEGVYFVEAAANCTLADVNHLERPLLLSKEDLVPCPTPEQWPVEPHNSLAMIQVTTFTCGGFVIGLRTNHAVADGTGAAQFMNAVGDLARGLPEPRVKPIWARDRFPDPDIKPGPLPELPVLPLQYIAFDFPAAYLGKLKAQYAATAGASKICSAFDIVIAKLWQCRTRAIAADPAAAVKLCFFASARQVLGLETGYWGNAIFPVKVSAAAGEVAASSVIELVGVVREAKRRMAGECLRWAEGRTGGADPFQMTFDYESVYVSDWSKLGFNDVDYGYGAPSAAGPLVNCDLISSVIVMRAPAPLAGTRLLASCVTKEHADDFAARMREDLV*